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Logistics for the regulation of higher neuronal function

Planned Research

Project AreaIntracellular logistics: interdisciplinary approaches to pathophysiology of membrane traffic
Project/Area Number 20113004
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionThe University of Tokushima

Principal Investigator

SASAKI Takuya  徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (40241278)

Project Period (FY) 2008 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥174,590,000 (Direct Cost: ¥134,300,000、Indirect Cost: ¥40,290,000)
Fiscal Year 2012: ¥31,980,000 (Direct Cost: ¥24,600,000、Indirect Cost: ¥7,380,000)
Fiscal Year 2011: ¥32,500,000 (Direct Cost: ¥25,000,000、Indirect Cost: ¥7,500,000)
Fiscal Year 2010: ¥31,980,000 (Direct Cost: ¥24,600,000、Indirect Cost: ¥7,380,000)
Fiscal Year 2009: ¥32,500,000 (Direct Cost: ¥25,000,000、Indirect Cost: ¥7,500,000)
Fiscal Year 2008: ¥45,630,000 (Direct Cost: ¥35,100,000、Indirect Cost: ¥10,530,000)
Keywords高次神経機能 / シナプス小胞 / シナプス形成 / 神経突起形成 / Rab13 / JRAB / アクチン細胞骨格 / ノックアウトマウス / 細胞内物流システム / Rabconnectin-3 / Actinin-4 / Rabファミリー低分子量G蛋白質 / 接着分子 / PC12細胞 / 分子内結合 / Rab3A
Research Abstract

Rab family small G proteins serve as molecular switches in the regulation of vesicle transport. In this study, we focus on the role and mode of action of Rab3A, Rab13, and their related proteins in higher neuronal functions. As to Rab3A, we found using the knockout mice for Rabconnectin, the Rab3A-related protein, is involved in neuronal function, probably through formation of synaptic organization. We also found that the Rab13-JRAB system regulates the reorganization of actin cytoskeleton during neuronal extension. Our results suggest that this system may simultaneously coordinate vesicle transport and actin cytoskeletal reorganization in neurons.

Report

(7 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report   Self-evaluation Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (52 results)

All 2012 2011 2010 2009 2008

All Journal Article (34 results) (of which Peer Reviewed: 26 results) Presentation (15 results) Book (3 results)

  • [Journal Article] Rab13 small G protein and Junctional Rab13-binding protein (JRAB) orchestrate actin cytoskeletal organization during epithelial Junctional development2012

    • Author(s)
      Sakane, A., Abdallah, A.A.M., Nakano, K., Honda, K., Ikeda, W., Nishikawa, Y., Matsumoto, M., Matsushita, N., Kitamura. T., and Sasaki, T
    • Journal Title

      J. Biol. Chem

      Volume: 287(51) Issue: 51 Pages: 42455-424680

    • DOI

      10.1074/jbc.m112.383653

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression of RASSF6 in kidney and the implication of RASSF6 and the Hippo pathway in the sorbitol-induced apoptosis in renal proximal tubular epithelial cells2012

    • Author(s)
      Withanage, K., Nakagawa, K., Ikeda, M., Kurihara, H., Kudo, T., Yang, Z., Sakane, A., Sasaki, T. and Hata, Y
    • Journal Title

      J. Biochem

      Volume: 152(1) Issue: 1 Pages: 111-119

    • DOI

      10.1093/jb/mvs056

    • NAID

      10031166119

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rab13 small G protein and Junctional Rab13-binding protein (JRAB) orchestrate actin cytoskeletal organization during epithelial junctional developmment.2012

    • Author(s)
      Sakane, A. et al.
    • Journal Title

      J.Biol.Chem.

      Volume: 287 Pages: 42455-42468

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of RASSF6 in kidney and the implication of RASSF6 and Hippo pathway in the sorbitol-induced apoptosis in renal proximal tubular epithelial cells.2012

    • Author(s)
      Withanage, K. et al.
    • Journal Title

      J.Biochem.

      Volume: 152 Pages: 111-119

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of RASSF6 in kidney and the implication of RASSF6 and the Hippo pathway in the sorbitol-induced apoptosis in renal proximal tubular epithelial cells2012

    • Author(s)
      Withanage K., et al
    • Journal Title

      J.Biocbem.

      Volume: (in press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rab13 regulates neurite outgrowth in PC12 cells through its effector protein, JRAB/MICAL-L22010

    • Author(s)
      Sakane, A., Honda, K. and Sasaki, T
    • Journal Title

      Mol. Cell. Biol

      Volume: 30(4) Issue: 4 Pages: 1077-1087

    • DOI

      10.1128/mcb.01067-09

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rubicon and PLEKHM1 negatively regulate the endocytic/autophagic pathway via a novel Rab7-binding domain2010

    • Author(s)
      Tabata, K., Matsunaga, K., Sakane, A., Sasaki, T., Noda, T. and Yoshimori, T
    • Journal Title

      Mol. Biol. Cell

      Volume: 21(23) Issue: 23 Pages: 4162-4173

    • DOI

      10.1091/mbc.e10-06-0495

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rabl3 regulates neurite outgrowth in PC12 cells through its effector protein2010

    • Author(s)
      Sakane A., Honda K., Sasaki, T.
    • Journal Title

      JRAB/MICAL-L2.Mol.Cell.Biol. 30

      Pages: 1077-1087

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Rubicon and PLEKHMI negatively regulate the endocytic/autophagic pathway via a novel Rab7-binding domain.2010

    • Author(s)
      Tabata, K.Sasaki, T., et al.(6人中4番目)
    • Journal Title

      Mol.Biol.Cell 21

      Pages: 4162-4173

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Rab13 regulates neurite outgrowth in PC12 cells through its effector protein, JRAB/MICAL-L2.2010

    • Author(s)
      Sakane A., et al.
    • Journal Title

      Mol.Cell.Biol.

      Volume: 30 Pages: 1077-1087

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rubicon and PLEKHM1 negatively regulate the endocytic/autophagic pathway via a novel Rab7-binding domain.2010

    • Author(s)
      Tabata, K., et al.
    • Journal Title

      Mol.Biol.Cell

      Volume: 21 Pages: 4162-4173

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rab13 regulates neurite outgrowth in PC12 cells throug its effector protein, JRAB/MICAL-L2.2010

    • Author(s)
      Sakane A., et al.
    • Journal Title

      Mol.Cell.Biol. 30

      Pages: 1077-1087

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rab family small G proteins in regulation of epithelial apical junctions2009

    • Author(s)
      Nishimura, N. and Sasaki, T
    • Journal Title

      Front. Biosci

      Volume: 14 Pages: 2115-2129

    • Related Report
      2012 Final Research Report
  • [Journal Article] APP anterograde transport requires Rab3A GTPase activity for assembly of the transport vesicle2009

    • Author(s)
      Szodorai, A., Kuan, Y-H., Hunzelmann, S., Engel, U., Sakane, A., Sasaki, T., Takai Y., Kirsch, J., Muller, U., Beyreuther, K., Brady, S., Morfini, G. and Kins, S
    • Journal Title

      J. Neurosci

      Volume: 29 (46) Issue: 46 Pages: 14534-14544

    • DOI

      10.1523/jneurosci.1546-09.2009

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] APP anterograde transport requires Rab3A GTPase activity for assembly of the transport vesicle.2009

    • Author(s)
      Szodorai, A.Sasaki, T., et al.(13人中6番目)
    • Journal Title

      J.Neurosci. 29

      Pages: 14534-14544

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Rab family small G proteins in regulation of epithelial apical junctions.2009

    • Author(s)
      Nishimura, N., Sasaki, T.
    • Journal Title

      Front.Biosci. 14

      Pages: 2115-2129

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] APP anterograde transport requires Rab3A GTPase activity forassembly of the transport vesicle.2009

    • Author(s)
      Szodorai, A., et al.
    • Journal Title

      J.Neurosci. 29

      Pages: 14534-14544

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RabファミリーSmallGタンパク質による小胞輪送制御と高次生命機能2009

    • Author(s)
      坂根亜由子, 他
    • Journal Title

      細胞工学 28巻

      Pages: 1247-1251

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Rab family small G proteins in regulation of epithelial apical junctions2009

    • Author(s)
      Nishimura, N.
    • Journal Title

      Front Biosci. 14

      Pages: 2115-2129

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Doc2α and Munc13-4 regulate Ca^<2+>-dependent secretory lysosome exocytosis in mast cells2008

    • Author(s)
      Higashio, H., Nishimura, N., Ishizaki, H., Miyoshi, J., Orita, S., Sakane, A. and Sasaki, T
    • Journal Title

      J. Immunol

      Volume: 180 (7) Pages: 4774-4784

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of actinin-4 in the recruitment of JRAB/MICAL-L2 to cell-cell junctions and the formation of functional tight junctions2008

    • Author(s)
      Nakatsuji, H., Nishimura, N., Yamamura, R., Kanayama, H. and Sasaki, T
    • Journal Title

      Mol. Cell. Biol

      Volume: 28 (10) Issue: 10 Pages: 3324-3335

    • DOI

      10.1128/mcb.00144-08

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Interaction of Rab3B with microtubule-binding protein Gas8 in NIH 3T3 cells2008

    • Author(s)
      Nishimura, N., Araki, K., Shinahara, W., Nakano, Y., Nishimura, K., Higashio, H. and Sasaki, T
    • Journal Title

      Arch. Biochem. Biophys

      Volume: 474(1) Issue: 1 Pages: 136-142

    • DOI

      10.1016/j.abb.2008.03.032

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cell-surface biotinylation to study endocytosis and recycling of occludin2008

    • Author(s)
      Nishimura, N. and Sasaki, T
    • Journal Title

      Methods Mol. Biol

      Volume: 440 Pages: 89-96

    • DOI

      10.1007/978-1-59745-178-9_7

    • ISBN
      9781588298652, 9781597451789
    • Related Report
      2012 Final Research Report
  • [Journal Article] Analysis on the emerging role of Rab3 GTPase-activating protein in Warburg Micro and Martsolf syndrome2008

    • Author(s)
      Sakane, A., Miyoshi, J., Takai, Y. and Sasaki, T
    • Journal Title

      Methods Enzymol

      Volume: 438 Pages: 131-139

    • DOI

      10.1016/s0076-6879(07)38009-9

    • Related Report
      2012 Final Research Report
  • [Journal Article] Identification and characterization of JRAB/MICAL-L2, a junctional Rab 13-binding protein2008

    • Author(s)
      Nishimura, N. and Sasaki, T
    • Journal Title

      Methods Enzymol

      Volume: 438 Pages: 141-153

    • DOI

      10.1016/s0076-6879(07)38010-5

    • Related Report
      2012 Final Research Report
  • [Journal Article] The interaction of JRAB/MICAL-L2 with Rab8 and Rab13 coordinates the assembly of tight junctions and adherens junctions2008

    • Author(s)
      Yamamura, R.
    • Journal Title

      Mol. Biol. Cell 19

      Pages: 971-983

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of Rab13 and JRAB/MICAL-L2 in epithelial cell scattering2008

    • Author(s)
      Kanda, I.
    • Journal Title

      Oncogene 27

      Pages: 1687-1695

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of Actinin-4 in the recruitment of JRAB/MICAL-L2 to cell-cell junctions and the formation of functional tight junctions2008

    • Author(s)
      Nakatsuji, H.
    • Journal Title

      Mol. Cell. Biol 28

      Pages: 3324-3335

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Doc 2α and Munc 13-4 regulate Ca^<2+>-dependent secretory lysosome exocytosis in mast cells2008

    • Author(s)
      Higashio, H.
    • Journal Title

      J.Immunol. 180

      Pages: 4774-4784

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Interaction of Rab 3B with microtubule-binding protein Gas8 in NIH 3T3 cells2008

    • Author(s)
      Nishimura, N.
    • Journal Title

      Arch. Biochem. Biophys. 474

      Pages: 136-142

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of epithelial cell adhesion and repulsion : role of endocytic recycling2008

    • Author(s)
      Nishimura, N.
    • Journal Title

      J. Med. Invest. 55

      Pages: 9-16

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cell-surface biotinylation to study endocytosis and recycling of occludin2008

    • Author(s)
      Nishimura, N.
    • Journal Title

      Methods Mol. Biol. 440

      Pages: 89-96

    • Related Report
      2008 Annual Research Report
  • [Journal Article] Analysis on the emerging role of Rab3 GTPase-activating protein in Warburg Micro and Martsolf syndrome2008

    • Author(s)
      Sakane, A.
    • Journal Title

      Methods Enzymol. 438

      Pages: 131-139

    • Related Report
      2008 Annual Research Report
  • [Journal Article] Identification and characterization of JRAB/MICAL-L2, a junctional Rab 13-binding protein2008

    • Author(s)
      Nishimura, N.
    • Journal Title

      Methods Enzymol. 438

      Pages: 141-153

    • Related Report
      2008 Annual Research Report
  • [Presentation] 動く細胞2012

    • Author(s)
      佐々木卓也, 坂根亜由子
    • Organizer
      ViEW2012 ビジョン技術の実利用ワークショップ
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2012-12-06
    • Related Report
      2012 Final Research Report
  • [Presentation] 細胞接着・運動において Rab13-JRAB系が制御する小胞輸送とアクチン細胞骨格再編成2011

    • Author(s)
      佐々木卓也, 坂根亜由子
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場(名古屋市)
    • Year and Date
      2011-10-03
    • Related Report
      2012 Final Research Report
  • [Presentation] 細胞接着・運動においてRab13-JRAB系が制御する小胞輸送とアクチン細胞骨格再編成2011

    • Author(s)
      坂根亜由子
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場(愛知県)
    • Year and Date
      2011-10-03
    • Related Report
      2011 Annual Research Report
  • [Presentation] A role of JRAB in epithelial junctional development: The cross-talk between vesicular trafficking and actin cytoskeleton in the submembrane plaque, 細胞間接着形成過程においてJRAB が制御する細胞内小胞輸送とアクチン細胞骨格系 の細胞膜直下でのクロストーク機構2011

    • Author(s)
      Ayuko Sakane and Takuya Sasaki
    • Organizer
      第63回日本細胞生物学会大会ミニシンポジウム
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2011-06-28
    • Related Report
      2012 Final Research Report
  • [Presentation] A role of JRAB in epithelial junctional development : The cross-talk between vesicular trafficking and actin cytoskeleton in the submembrane plaque2011

    • Author(s)
      Ayuko Sakane
    • Organizer
      第63回日本細胞生物学会大会ミニシンポジウム
    • Place of Presentation
      北海道大学(北海道)(招待講演)
    • Year and Date
      2011-06-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] Exocytotic Rab small G proteins regulate neuronal development and plasticity.2010

    • Author(s)
      Takuya Sasaki, Ayuko Sakane
    • Organizer
      BMB2008シンポジウム(第31回日本分 子生物学会年会・第81回日本生化学会大 会合同大会)
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-11
    • Related Report
      2010 Self-evaluation Report
  • [Presentation] Role of Rab small G proteins in cellular morphogenesis.2010

    • Author(s)
      Takuya Sasaki, et al.
    • Organizer
      Sapporo International Cancer Symposium 2010
    • Place of Presentation
      札幌市
    • Year and Date
      2010-06-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] Functions of Rab family small G proteins in neurite outgrowth. 神経突起形成における Rabファミリー低分子量G蛋白質の機能2010

    • Author(s)
      Takuya Sasaki and Ayuko Sakane
    • Organizer
      第62回日本細胞生物学会大会シンポジウム
    • Place of Presentation
      大阪国際会議場(大阪市)
    • Year and Date
      2010-05-20
    • Related Report
      2012 Final Research Report
  • [Presentation] Functions of Rab family small G proteins in neurite outgrowth.2010

    • Author(s)
      Takuya Sasaki, Ayuko Sakane
    • Organizer
      第62回日本細胞生物学会大会シンポジウム
    • Place of Presentation
      大阪
    • Year and Date
      2010-05-20
    • Related Report
      2010 Self-evaluation Report
  • [Presentation] Functions of Rab family small G proteins in neurite outgrowth.2010

    • Author(s)
      Takuya Sasaki, et al.
    • Organizer
      第62回日本細胞生物学会大会シンポジウム
    • Place of Presentation
      大阪市
    • Year and Date
      2010-05-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] The Rubicon family negatively regulates the endocytic pathway through the interactions with Rab7, Rubiconファミリーは Rab7との結合を介してエンドサイトーシス経路を負に制御する2010

    • Author(s)
      Keisuke Tabata, Kohichi Matsunaga, Ayuko Sakane, Takuya Sasaki, Takeshi Noda and Tamotsu Yoshimori
    • Organizer
      第62回日本細胞生物学会大会
    • Place of Presentation
      大阪国際会議場(大阪市)
    • Year and Date
      2010-05-19
    • Related Report
      2012 Final Research Report
  • [Presentation] The Rubicon family negatively regulates the endocytic pathway through the interactions with Rab7.2010

    • Author(s)
      Tabata K. Sasaki T., et al.(6人中4番目)
    • Organizer
      第62回日本細胞生物学会大会
    • Place of Presentation
      大阪
    • Year and Date
      2010-05-19
    • Related Report
      2010 Self-evaluation Report
  • [Presentation] The Rubicon family negatively regulates the endocytic pathway through the interactions with Rab7.2010

    • Author(s)
      Keisuke Tabata, et al.
    • Organizer
      第62回日本細胞生物学会大会
    • Place of Presentation
      大阪市
    • Year and Date
      2010-05-19
    • Related Report
      2010 Annual Research Report
  • [Presentation] Role of Rab small G proteins in cellular morphogenesis.2010

    • Author(s)
      Takuya Sasaki, Ayuko Sakane
    • Organizer
      Sapporo International Cancer Symposium
    • Place of Presentation
      Sapporo
    • Related Report
      2010 Self-evaluation Report
  • [Presentation] 高次細胞機能発現におけるエキソサイトーシス系Rabファミリー低分子量G蛋白質の作用機構2008

    • Author(s)
      佐々木卓也
    • Organizer
      第60回日本細胞生物学会大会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2008-06-30
    • Related Report
      2008 Annual Research Report
  • [Book] 生体の科学(Rab3Aと神経可塑性) 61巻5号2010

    • Author(s)
      坂根亜由子, 佐々木卓也
    • Related Report
      2010 Self-evaluation Report
  • [Book] Rab3Aと神経可塑性2010

    • Author(s)
      坂根亜由子, 他
    • Publisher
      生体の科学
    • Related Report
      2010 Annual Research Report
  • [Book] 細胞工学(RabファミリーSmall Gタンパク質による小胞輸送制御と高次生命機能) 28巻12号2009

    • Author(s)
      坂根亜由子, 佐々木卓也
    • Related Report
      2010 Self-evaluation Report

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Published: 2008-04-01   Modified: 2018-03-28  

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