|Budget Amount *help
¥174,590,000 (Direct Cost: ¥134,300,000、Indirect Cost: ¥40,290,000)
Fiscal Year 2012: ¥31,980,000 (Direct Cost: ¥24,600,000、Indirect Cost: ¥7,380,000)
Fiscal Year 2011: ¥32,500,000 (Direct Cost: ¥25,000,000、Indirect Cost: ¥7,500,000)
Fiscal Year 2010: ¥31,980,000 (Direct Cost: ¥24,600,000、Indirect Cost: ¥7,380,000)
Fiscal Year 2009: ¥32,500,000 (Direct Cost: ¥25,000,000、Indirect Cost: ¥7,500,000)
Fiscal Year 2008: ¥45,630,000 (Direct Cost: ¥35,100,000、Indirect Cost: ¥10,530,000)
We aimed to identify molecular mechanism and functional significance of granule docking to the plasma membrane in regulated exocytosis. For this purpose, we first analyzed the function of the Rab27 effector granuphilin and its partner, syntaxin-la, using gene-knockout mice. We found that only granuphilin, but not syntaxin-la, is essential for the docking process. We also established the microscopic system to simultaneously observe behavior of granules and associated molecules in living cells to explore the relationship between granule docking and fusion. Furthermore, we discovered the roles of other Rab27 effectors in regulated exocytosis: exiphilin7 functions in the exocytosis of undocked granules, whereas exophilin8 is involved in the recruitment of granules into the cortical actin network.