|Budget Amount *help
¥126,230,000 (Direct Cost: ¥97,100,000、Indirect Cost: ¥29,130,000)
Fiscal Year 2012: ¥21,060,000 (Direct Cost: ¥16,200,000、Indirect Cost: ¥4,860,000)
Fiscal Year 2011: ¥21,060,000 (Direct Cost: ¥16,200,000、Indirect Cost: ¥4,860,000)
Fiscal Year 2010: ¥21,060,000 (Direct Cost: ¥16,200,000、Indirect Cost: ¥4,860,000)
Fiscal Year 2009: ¥29,380,000 (Direct Cost: ¥22,600,000、Indirect Cost: ¥6,780,000)
Fiscal Year 2008: ¥33,670,000 (Direct Cost: ¥25,900,000、Indirect Cost: ¥7,770,000)
We explored the principle that governs the packaging of DNA in the cell nucleus. The biggest finding was as follows: in yeast, the paths of nucleosomal arrays and the chromatin architectures are determined principally by the physical properties of genomic DNA and the size of the nucleus. The second biggest finding was that DNA sequence-based selective association occurs between nucleosomes with identical DNA sequences. Thus, physical properties of DNA, DNA sequence-based selective association between nucleosomes and the nucleus size are critical determinants in the genome compaction in the nucleus. Furthermore, we also found that most of the methylation introduced into DNA induces changes in conformation or physical-properties of the methylated sites, indicating some role of DNA methylation in the genome compaction in the nucleus.