|Budget Amount *help
¥130,130,000 (Direct Cost: ¥100,100,000、Indirect Cost: ¥30,030,000)
Fiscal Year 2012: ¥21,840,000 (Direct Cost: ¥16,800,000、Indirect Cost: ¥5,040,000)
Fiscal Year 2011: ¥21,840,000 (Direct Cost: ¥16,800,000、Indirect Cost: ¥5,040,000)
Fiscal Year 2010: ¥44,330,000 (Direct Cost: ¥34,100,000、Indirect Cost: ¥10,230,000)
Fiscal Year 2009: ¥22,750,000 (Direct Cost: ¥17,500,000、Indirect Cost: ¥5,250,000)
Fiscal Year 2008: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Heterochromatin Protein 1 (HP1) is thought to play a role in compacted heterochromatin formation, through binding to K9 trimethylated histone H3 and its interacting proteins. By proteomic analysis, we identified 82 HP1 binding proteins (HPBPs) in human cells. In addition to known HPBPs, several uncharacterized and/or unexpected proteins were identified [Nature Cell Biol. 12: 719, 2010]. Interestingly, an identified HP1 binding protein was enriched in inactive X chromosomes (Xi), in association with SMCHD1, and thus we named it HBiX1 (HP1 binding protein enriched in Xi). Cytological and epigenomic analyses revealed that HBiX1 and SMCHD1 mediate the compaction of Xi to form heterochromatin structure, by linking the H3K9me3 domains and the XIST/H3K27me3 domains [Nature Struct. & Mol. Biol. 20: 566, 2013]. These studies uncovered that new molecular insights of heterochromatin, especially, how epigenetic marks are translated into higher order chromatin structure to repress transcription.