|Budget Amount *help
¥85,280,000 (Direct Cost: ¥65,600,000、Indirect Cost: ¥19,680,000)
Fiscal Year 2012: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
Fiscal Year 2011: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
Fiscal Year 2010: ¥15,600,000 (Direct Cost: ¥12,000,000、Indirect Cost: ¥3,600,000)
Fiscal Year 2009: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2008: ¥31,070,000 (Direct Cost: ¥23,900,000、Indirect Cost: ¥7,170,000)
We succeeded in the reconstitution of GSC/niche system in Drosophila. We established stable cell lines originated from female and male niche cells (cap cells and escort cells in female, and hub and cyst cells in male). Using these cell lines, we identified highly expressed genes in each cell line and found that there are many unique genes specifically expressed in each niche cell line. Furthermore, we found that Drosophila insulin-like genes are also expressed in the niche cells. We constructed co-culture system of GSCs and niche cells and analyzed roles of growth factors. Furthermore, we succeeded in artificial induction of the differentiation and dedifferentiation of GSCs by coculturing with niche cells.