|Budget Amount *help
¥111,540,000 (Direct Cost: ¥85,800,000、Indirect Cost: ¥25,740,000)
Fiscal Year 2012: ¥21,320,000 (Direct Cost: ¥16,400,000、Indirect Cost: ¥4,920,000)
Fiscal Year 2011: ¥21,320,000 (Direct Cost: ¥16,400,000、Indirect Cost: ¥4,920,000)
Fiscal Year 2010: ¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000)
Fiscal Year 2009: ¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000)
Fiscal Year 2008: ¥24,700,000 (Direct Cost: ¥19,000,000、Indirect Cost: ¥5,700,000)
We established some new protein adductions of proteins by lipid-derived electrophilic aldehydes. To verify the presence of the protein-bound ligands in vivo, we raised a monoclonal antibody against the ligands, characterized the epitopes, and immunohistochemically demonstrated the presence of the ligands in vivo. In addition, we developed high performance liquid chromatography with on-line electrospray ionization tandem mass spectrometry (LC/ESI/MS/MS)-based quantification methods and showed evidence that the ligands are indeed accumulated in vivo. We also established the danger signal function of these ligands. Furthermore, we characterized the multi-specificity mechanism of innate antibodies that recognize the modified proteins as antigenic ligands and established that they contribute to the removal of apoptotic cells and biological defense.