|Budget Amount *help
¥109,070,000 (Direct Cost: ¥83,900,000、Indirect Cost: ¥25,170,000)
Fiscal Year 2013: ¥21,970,000 (Direct Cost: ¥16,900,000、Indirect Cost: ¥5,070,000)
Fiscal Year 2012: ¥21,970,000 (Direct Cost: ¥16,900,000、Indirect Cost: ¥5,070,000)
Fiscal Year 2011: ¥21,320,000 (Direct Cost: ¥16,400,000、Indirect Cost: ¥4,920,000)
Fiscal Year 2010: ¥21,320,000 (Direct Cost: ¥16,400,000、Indirect Cost: ¥4,920,000)
Fiscal Year 2009: ¥22,490,000 (Direct Cost: ¥17,300,000、Indirect Cost: ¥5,190,000)
Self-incompatibility (SI) in the Brassicaceae is controlled by the S-haplotype-specific interaction between pollen ligand SP11 and its stigmatic receptor SRK. SP11/SCR binding to cognate SRK induces autophosphorylation of SRK, which triggers a signalling cascade leading to the rejection of self-pollen. Here we introduced calcium sensor protein Yellow Cameleon 3.60 gene into self-incompatible Arabidopsis thaliana transgenic plants expressing both SP11 and SRK. Self-pollination induced an increase of [Ca2+]cyt in papilla cells. The [Ca2+]cyt increase was also induced in papilla cell-protoplasts by directly applying the cognate SP11/SCR. Furthermore, we found that wild type-pollen hydration could also be arrested by the [Ca2+]cyt increase resulting by microinjection of concentrated CaCl2 solution. These results suggest that the SI-induced [Ca2+]cyt increase is a downstream event of SI signal transduction pathway that triggers rejection of self-pollen.