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Authentication mechanism of mammalian sperm in the female reproductive tract

Planned Research

Project AreaElucidation of Common Mechanisms for Allogeneic Authentication in Animals and Plants
Project/Area Number 21112005
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionUniversity of Tsukuba

Principal Investigator

BABA TADASHI  筑波大学, 生命環境系, 教授 (40165056)

Project Period (FY) 2009-07-23 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥114,790,000 (Direct Cost: ¥88,300,000、Indirect Cost: ¥26,490,000)
Fiscal Year 2013: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2012: ¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000)
Fiscal Year 2011: ¥21,450,000 (Direct Cost: ¥16,500,000、Indirect Cost: ¥4,950,000)
Fiscal Year 2010: ¥22,490,000 (Direct Cost: ¥17,300,000、Indirect Cost: ¥5,190,000)
Fiscal Year 2009: ¥30,160,000 (Direct Cost: ¥23,200,000、Indirect Cost: ¥6,960,000)
Keywordsマウス / 精子 / 卵子 / 卵管 / 卵丘細胞塊
Research Abstract

This study describes the authentication mechanism of fertilizing sperm during migration through the female reproductive tract. We have successfully identified a sperm ADAM3-interacting membrane protein localized on epithelial cells in the uterus and oviduct. Functional analysis of the membrane protein suggests a possible involvement in release of sperm from the epithelial cells. We also found that double-knockout mice lacking two sperm serine proteases, ACR and PRSS21, are subfertile, although the mutant epididymal sperm fail to undergo the acrosome reaction on the zona surface and to traverse the zona pellucida in vitro. Moreover, We have identified a novel proteasome subunit specifically present in sperm. The function of the mouse sperm proteasome may be distinguished from sea urchin and starfish proteasomes by functional diversification.

Report

(6 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (54 results)

All 2013 2012 2011 2010 2009 Other

All Journal Article (17 results) (of which Peer Reviewed: 17 results) Presentation (31 results) (of which Invited: 1 results) Remarks (6 results)

  • [Journal Article] Two functional forms of ACRBP/sp32 are produced by pre-mRNA alternative splicing in the mouse2013

    • Author(s)
      Kanemori, Y., Ryu, J.-H., Sudo, M., Niida-Araida, Y., Kodaira, K., Takenaka, M., Kohno, N., Sugiura, S., Kashiwabara, S., and Baba, T.
    • Journal Title

      Biol. Reprod

      Volume: 88 Issue: 4 Pages: 1-8

    • DOI

      10.1095/biolreprod.112.107425

    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional characterization of double-knockout mouse sperm lacking SPAM1 and ACR or SPAM1 and PRSS21 in fertilization2012

    • Author(s)
      Zhou, C., Kang, W., and Baba, T.
    • Journal Title

      J. Reprod. Dev

      Volume: 58 Pages: 330-337

    • NAID

      10030821330

    • URL

      https://www.jstage.jst.go.jp/article/jrd/58/3/58_2011-006/_article

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Phosphatidylinositol 4-phosphate 5-kinase is indispensable for mouse spermatogenesis2012

    • Author(s)
      Hasegawa, H., Noguchi, J., Yamashita, M., Okada, R., Sugimoto, R., Furuya, M., Unoki, T., Funakoshi, Y., Baba, T., and Kanaho, Y.
    • Journal Title

      Biol. Reprod

      Volume: 86 Issue: 5 Pages: 1-12

    • DOI

      10.1095/biolreprod.110.089896

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional characterization of double-knockout mouse sperm lacking SPAM1 and ACR or SPAM1 and PRSS21 in fertilization2012

    • Author(s)
      Zhou, C., Kang, W., and Baba, T.
    • Journal Title

      J. Reprod. Dev.

      Volume: 58 Pages: 330-337

    • NAID

      10030821330

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional characterization of double-knockout mouse sperm lacking SPAM1 and ACR or SPAM1 and PRSS21 in fertilization2012

    • Author(s)
      Chong Zhou
    • Journal Title

      Journal of Reproduction and Development

      Volume: 58 Pages: 330-337

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Two functional forms of ACRBP/sp32 are produced by pre-mRNA alternative splicing in the mouse2012

    • Author(s)
      Yoshinori Kanemori
    • Journal Title

      Biology of Reproduction

      Volume: 印刷中

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Possible involvement of mitogen- and stress-activated protein kinase 1, MSK1, in metaphase-ll arrest through phosphorylation of EMI2 in mouse oocytes2011

    • Author(s)
      Miyagaki, Y., Kanemori, Y., and Baba, T.
    • Journal Title

      Dev. Biol

      Volume: 359 Issue: 1 Pages: 73-81

    • DOI

      10.1016/j.ydbio.2011.08.021

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression analysis of MIF4GD in the rat testis2011

    • Author(s)
      Okada, K., Kimura, M., Moriyama, Y., Nakai, M., Kikuchi, K., Kaneko, H., Kunieda, T., Baba, T., and Noguchi, J.
    • Journal Title

      J. Reprod. Dev

      Volume: 57 Pages: 256-261

    • NAID

      10028275441

    • URL

      https://www.jstage.jst.go.jp/article/jrd/57/2/57_10-138H/_article

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional analysis of MIF4GD in the rat testis2011

    • Author(s)
      Kenzo Okada
    • Journal Title

      Journal of Reproduction and Development

      Volume: 57 Pages: 256-261

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Protein disulfide isomerase-P5, down-regulated in the final stage of boar epididymal sperm maturation, catalyzes disulfide formation to inhibit protein function in oxidative refolding of reduced denatured lysozyme.2010

    • Author(s)
      Akama K, Horikoshi T, Sugiyama A, Nakahata S, Akitsu A, Niwa N, Intoh A, Kakui Y, Sugaya M, Takei K, Imaizumi N, Sato T, Matsumoto R, Iwahashi H, Kashiwabara SI, Baba T, Nakamura M, Toda T.
    • Journal Title

      Biochim Biophys Acta.

      Volume: 1804(6) Issue: 6 Pages: 1272-1284

    • DOI

      10.1016/j.bbapap.2010.02.004

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mice lacking two sperm serine proteases, ACR and PRSS21, are subfertile, but the mutant sperm are infertile in vitro2010

    • Author(s)
      Kawano, N., Kang, Wj., Yamashita, M., Koga, Y., Yamazaki, T., Hata, T., Miyado, K., and Baba, T.
    • Journal Title

      Biol. Reprod

      Volume: 83 Issue: 3 Pages: 359-369

    • DOI

      10.1095/biolreprod.109.083089

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mice lacking two sperm serine proteases, ACR and PRSS21, are subfertile, but the mutant sperm are infertile in vitro.2010

    • Author(s)
      Kawano N., Kang, W., Yamashita, M., Koga, Y., Yamazaki, T., Hata, T., Miyado, M., Baba, T.
    • Journal Title

      Biology of Reproduction

      Volume: 83 Pages: 359-369

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Protein disulfide isomerase-P5, down-regulated in the final stage of boar epididymal sperm maturation, catalyzes disulfide formation to inhibit protein function in oxidative refolding of reduced denatured lysozyme.2010

    • Author(s)
      Akama, K., Horikoshi, T., Sugiyama, A., Nakahata, S.Akitsu, A., Niwa, N., Intoh, A., Kakui, Y., Sugaya, M.Takei, K., Imaizumi, N., Sato, T., Matsumoto, R., Iwahashi, H., Kashiwabara, S., Baba, T., Nakamura, M., Toda, T.
    • Journal Title

      Biochim.Biophys.Acta

      Volume: 1804 Pages: 1274-1284

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hyaluronan-degrading activity of mouse sperm hyaluronidase is not required for fertilization?2009

    • Author(s)
      Kang,WJ., Zhou, C., Koga, Y., and Baba, T.
    • Journal Title

      J. Reprod. Dev

      Volume: 56 Pages: 140-144

    • NAID

      10026323923

    • URL

      https://www.jstage.jst.go.jp/article/jrd/56/1/56_09-152N/_article

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional roles of sperm hyaluronidase, HYAL5 and SPAM1, in mouse fertilization2009

    • Author(s)
      Kimura, M., Kim, E., Kang,WJ., Yamashita, M., Saigo, M., Yamazaki, T., Nakanishi, T., Kashiwabara, S., and Baba, T.
    • Journal Title

      Biol. Reprod

      Volume: 81 Issue: 5 Pages: 939-947

    • DOI

      10.1095/biolreprod.109.078816

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional roles of mouse sperm hyaluronidases, HYAL5 and SPAM1, in fertilization.2009

    • Author(s)
      Kimura, M., Kim, E., Kang, W., Yamashita, M., Saigo, M., Yamazaki, T., Nakanishi, T., Kashiwabara, S., Baba, T.
    • Journal Title

      Biology of Reproduction

      Volume: 81 Pages: 939-947

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hyaluronan-degrading activity of mouse sperm hyaluronidase is not required for fertilization?2009

    • Author(s)
      Kang, W., Zhou, C., Koga, Y., Baba, T.
    • Journal Title

      Journal of Reproduction and Development

      Volume: 56 Pages: 140-144

    • NAID

      10026323923

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 精子アクロソームタンパク質ACRBP とACRのダブル欠損マウスの解析2013

    • Author(s)
      高嶋明日登,兼森芳紀,首藤舞,康宇鎮,馬場忠
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2013-12-04
    • Related Report
      2013 Final Research Report
  • [Presentation] Two functional forms of ACRBP/sp32 are produced by pre-mRNA alternative splicing in mouse spermatogenesis2013

    • Author(s)
      Sudo, M., Kanemori, Y., Ryu, JH., Niida-Araida, Y., Kodaira, K., Takenaka, M., Kohno, N., Sugiura, S., Kashiwabara, S., and Baba, T.
    • Organizer
      Gordon Research Conference Program on Fertilization & Activation of Development
    • Place of Presentation
      Holderness School Holderness, NH, USA
    • Year and Date
      2013-07-15
    • Related Report
      2013 Final Research Report
  • [Presentation] Proacrosin-binding protein ACRBP/sp32 regulates acrosome formation during mouse spermiogenesis2013

    • Author(s)
      Kanemori, Y., Sudo, M., Koga, Y., Okabe, M., and Baba, T.
    • Organizer
      Gordon Research Conference Program on Fertilization &Activation of Development
    • Place of Presentation
      Holderness School Holderness, NH, USA
    • Year and Date
      2013-07-15
    • Related Report
      2013 Final Research Report
  • [Presentation] Functional role of mouse ACRBP/sp32 in spermatogenesis and fertilization2012

    • Author(s)
      柳 辰協、首藤 舞、兼森 芳紀、柏原 真一、馬場 忠
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2012-12-14
    • Related Report
      2013 Final Research Report
  • [Presentation] Sperm penetration through the cumulus matrix2012

    • Author(s)
      Baba, T
    • Organizer
      International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants
    • Place of Presentation
      名古屋ガーデンパレス
    • Year and Date
      2012-11-13
    • Related Report
      2013 Final Research Report
  • [Presentation] Localization of a PSMA8-containing proteasome in mouse sperm2011

    • Author(s)
      Koyama, M., Koga, Y., Hyodo, H., Yamashita, M., Ogichi, K., Kanemori, Y., Sawada H., and Baba, T.
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2011-12-13
    • Related Report
      2013 Final Research Report
  • [Presentation] 精子の卵丘細胞層通過でのヒアルロニダーゼとセリンプロテアーゼによる協働的な作用2011

    • Author(s)
      周崇、康宇鎭、馬場忠
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2011-12-13
    • Related Report
      2013 Final Research Report
  • [Presentation] Characterization of a novel PSMA8-containing proteasome present in mouse sperm2011

    • Author(s)
      Koga, Y., Koyama, M., Hyodo, H., Yamashita, M., Sawada, H., Baba, T.
    • Organizer
      World Congress on Reproductive Biology
    • Place of Presentation
      ケアンズコンベンションセンター
    • Year and Date
      2011-10-10
    • Related Report
      2013 Final Research Report
  • [Presentation] Acrosome reaction of mouse epididymal sperm facilitates the entry into the cumulus mass2011

    • Author(s)
      Kang,Wj., Zhou, C., Yamashita, M., and Baba, T.
    • Organizer
      動植物アロ認証 第3回領域会議
    • Place of Presentation
      関西セミナーハウス、京都市
    • Year and Date
      2011-06-30
    • Related Report
      2013 Final Research Report
  • [Presentation] Fuctional Analysis of PABPC2 in Mouse Testis2011

    • Author(s)
      Miyagaki, Y., Kimura, M., Ishida, K., Kashiwabara SI., and Baba, T.
    • Organizer
      2011 XXIst North American TestisWorkshop
    • Place of Presentation
      Montrral QC, Canada
    • Year and Date
      2011-03-31
    • Related Report
      2013 Final Research Report
  • [Presentation] 受精とプロテオリシス2010

    • Author(s)
      馬場 忠
    • Organizer
      第33回日本分子生物学会年会ワークショップ「動植物に共通するアロ認証中核原理を探る」
    • Place of Presentation
      神戸ポートピアホテル
    • Year and Date
      2010-12-07
    • Related Report
      2013 Final Research Report 2010 Annual Research Report
  • [Presentation] The trypsin-like serine protease activity of ACR and PRSS21 is important but not essential for fertilization in vivo2010

    • Author(s)
      Kang, Wj., Kawano, N., Yamashita, M., Koga, Y., Yamazaki, T., Hata, T., Miyado, k., and Baba, T.
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2010-12-07
    • Related Report
      2013 Final Research Report
  • [Presentation] The trypsin-like serine protease activity of ACR and PRSS21 is important but not essential for fertilization in vivo2010

    • Author(s)
      Kang,Wj., Kawano, N., Yamashita, M., Koga, Y., Yamazaki, T., Hata, T., Miyado, K., and Baba, T.
    • Organizer
      Illinois Symposium on Reproductive Sciences in Health and Disease
    • Place of Presentation
      University of Illinois, Chicago, USA
    • Year and Date
      2010-10-11
    • Related Report
      2013 Final Research Report
  • [Presentation] Possible role of mitogen- and stress-activated protein kinase-1 (MSK1) in meiotic arrest of mouse oocytes2010

    • Author(s)
      Miyagaki, Y., Kanemori, Y., and Baba, T.
    • Organizer
      Gordon Research Conference
    • Place of Presentation
      New Hampshire, USA
    • Year and Date
      2010-08-16
    • Related Report
      2013 Final Research Report
  • [Presentation] Effects of placental scars on implantation in multiparous mice2010

    • Author(s)
      Yamashita, M., and Baba, T.
    • Organizer
      Gordon Research Conference Program on Reproductive Tracts Biology
    • Place of Presentation
      New Hampshire, USA
    • Year and Date
      2010-08-16
    • Related Report
      2013 Final Research Report
  • [Presentation] Functional analysis of sperm proteasome in mouse fertilization2009

    • Author(s)
      Hyodo, H., Yamashita, M., Izumi, Y., Sawada, H., and Baba, T.
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-10
    • Related Report
      2013 Final Research Report
  • [Presentation] Hyaluronan-degrading activity of mouse sperm hyaluronidase is not required for fertilization?2009

    • Author(s)
      Kang,WJ., Zhou, C., Koga, Y., and Baba, T.
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-10
    • Related Report
      2013 Final Research Report
  • [Presentation] Recognition system of mammalian sperm in the female reproductive tract2009

    • Author(s)
      Baba, T.
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-09
    • Related Report
      2013 Final Research Report
  • [Presentation] Target proteins of SBTI in sperm acrosome reaction2009

    • Author(s)
      Izumi, Y., Yamashita, M., Hyodo, H., Kang, WJ., and Baba, T.
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-09
    • Related Report
      2013 Final Research Report
  • [Presentation] Role of hyaluronidase in sperm penetration through cumulus matrix2009

    • Author(s)
      Baba, T.
    • Organizer
      20^<th> International Symposium on Glycoconjugates
    • Place of Presentation
      San Juan, Puerto Rico (USA)
    • Year and Date
      2009-11-30
    • Related Report
      2013 Final Research Report
  • [Presentation] Functional roles of sperm hyaluronidases, HYAL5 and SPAM1, in Mouse fertilization.2009

    • Author(s)
      Tadashi Baba
    • Organizer
      20th International Symposium on Glycobiology
    • Place of Presentation
      Puerto Rico
    • Related Report
      2009 Annual Research Report
  • [Presentation] Two functional forms of ACRBP/sp32 are produced by pre-mRNA alternative splicing in mouse spermatogenesis

    • Author(s)
      Sudo, M., Kanemori, Y., Ryu, JH., Niida-Araida, Y., Kodaira, K., Takenaka, M., Kohno, N., Sugiura, S., Kashiwabara, SI., and Baba, T.
    • Organizer
      Gordon Research Conference Program on Fertilization & Activation of Development.
    • Place of Presentation
      Holderness School Holderness, NH, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] Proacrosin-binding protein ACRBP/sp32 regulates acrosome formation during mouse spermiogenesis

    • Author(s)
      Kanemori, Y., Sudo, M., Koga, Y., Okabe, M., and Baba, T.
    • Organizer
      Gordon Research Conference Program on Fertilization & Activation of Development.
    • Place of Presentation
      Holderness School Holderness, NH, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] 精子アクロソームタンパク質ACRBP とACR のダブル欠損マウスの解析

    • Author(s)
      高嶋 明日登,兼森 芳紀,首藤 舞,康 宇鎮,馬場 忠
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] Sperm penetration through the cumulus matrix

    • Author(s)
      馬場 忠
    • Organizer
      International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants
    • Place of Presentation
      名古屋ガーデンパレス
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Functional role of mouse ACRBP/sp32 in spermatogenesis and fertilization

    • Author(s)
      柳 辰協、首藤 舞、兼森 芳紀、柏原 真一、馬場 忠
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Annual Research Report
  • [Presentation] 精子の卵丘細胞層通過でのヒアルロニダーゼとセリンプロテアーゼによる協働的な作用

    • Author(s)
      周 崇
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2011 Annual Research Report
  • [Presentation] Localization of a PSMA8-containing proteasome in mouse sperm

    • Author(s)
      Mana Koyama
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2011 Annual Research Report
  • [Presentation] Proacrosin-binding protein sp32/ACRBP is involved in acrosome biogenesis of mouse sperm

    • Author(s)
      Jin Hyeob Ryu
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2011 Annual Research Report
  • [Presentation] Characterization of a novel PSMA8-containing proteasome present in mouse sperm

    • Author(s)
      Mana Koyama
    • Organizer
      World Congress on Reproductive Biology
    • Place of Presentation
      Cairns Convention Center, Australia
    • Related Report
      2011 Annual Research Report
  • [Presentation] Identification and Characterization of SDK2S, a truncated form of cell adhesion protein SDK2 in mouse oviduct

    • Author(s)
      Jae Hwa Lee
    • Organizer
      11th World Congress on Controversies in Obstetrics, Gynecology & Infertility
    • Place of Presentation
      Le Meridien Montparnasse Hotel, France
    • Related Report
      2011 Annual Research Report
  • [Remarks] 馬場 忠研究室

    • URL

      http://www.acroman.org/

    • Related Report
      2013 Final Research Report
  • [Remarks] 馬場 忠研究室ホームページ

    • URL

      http://www.acroman.org/

    • Related Report
      2013 Annual Research Report
  • [Remarks] 馬場 忠研究室ホームページ

    • URL

      http://www.acroman.org/

    • Related Report
      2012 Annual Research Report
  • [Remarks] 研究室webページ

    • URL

      http://www.acroman.org/

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.acroman.org/

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.acroman.org/

    • Related Report
      2009 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2019-07-29  

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