|Budget Amount *help
¥79,430,000 (Direct Cost: ¥61,100,000、Indirect Cost: ¥18,330,000)
Fiscal Year 2013: ¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2012: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2011: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2010: ¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000)
Fiscal Year 2009: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
The aim of this study is elucidating the molecular mechanism of sperm-egg fusion. In the first half of the period, the analysis of functional domains in GCS1 protein, which is a gamete fusion factor conserved in plants, animals and protists, was performed. As a result, it was found that the functional GCS1 domain resides only in the N-terminus, and that the C-terminus following the transmembrane domain is dispensable for GCS1 function. Besides, in the last half, the functional analyses of GEX2, which was newly identified as a plant fertilization factor, was performed. As a result, it was found that Arabidopsis GEX2 mutant sperm cells could not be attached with female gametes, suggesting that GEX2 functions in gamete attachment for successful fertilization.