| Budget Amount *help |
¥244,790,000 (Direct Cost: ¥188,300,000、Indirect Cost: ¥56,490,000)
Fiscal Year 2013: ¥39,260,000 (Direct Cost: ¥30,200,000、Indirect Cost: ¥9,060,000)
Fiscal Year 2012: ¥39,260,000 (Direct Cost: ¥30,200,000、Indirect Cost: ¥9,060,000)
Fiscal Year 2011: ¥39,260,000 (Direct Cost: ¥30,200,000、Indirect Cost: ¥9,060,000)
Fiscal Year 2010: ¥39,260,000 (Direct Cost: ¥30,200,000、Indirect Cost: ¥9,060,000)
Fiscal Year 2009: ¥87,750,000 (Direct Cost: ¥67,500,000、Indirect Cost: ¥20,250,000)
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| Research Abstract |
In order to understand functionally important intrinsically disordered proteins (IDPs) from their structure and to clarify their target recognition mechanisms, characterization of dynamic structures of IDPs was carried out using NMR and mass spectrometry (MS). The project achievements are (1) Analysis of gas-phase behaviors of the histone multimers and homologous recombination related factor Swi5-Sfr1 complexes, (2) NMR structural analysis of iPS cell-inducing transcription factors Sox2 and Oct3/4, (3) Structure determination of the complex of splicing factor PQBP1and spliceosomal protein U5-15kD and PQBP1-U5-15kD-U5-52K, (4) Identification of two possible inhibitors of NRSF-Sin3 interaction based on the NMR complex structure, (5) Development of a fast and accurate fitting program of NMR relaxation dispersion data, and (6) NMR analysis of the complex of chromodomain of HP1 and an H3K9me peptide.
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