|Budget Amount *help
¥207,220,000 (Direct Cost: ¥159,400,000、Indirect Cost: ¥47,820,000)
Fiscal Year 2013: ¥38,350,000 (Direct Cost: ¥29,500,000、Indirect Cost: ¥8,850,000)
Fiscal Year 2012: ¥38,350,000 (Direct Cost: ¥29,500,000、Indirect Cost: ¥8,850,000)
Fiscal Year 2011: ¥40,300,000 (Direct Cost: ¥31,000,000、Indirect Cost: ¥9,300,000)
Fiscal Year 2010: ¥40,300,000 (Direct Cost: ¥31,000,000、Indirect Cost: ¥9,300,000)
Fiscal Year 2009: ¥49,920,000 (Direct Cost: ¥38,400,000、Indirect Cost: ¥11,520,000)
Our research has been focusing on the relationships between the chromatin structure and the protein factors involved in DNA replication, repair, and transcription. Many proteins in these pathways contains intrinsically disordered regions (IDR), which should have important functions to process the DNA metabolisms precisely. In this research project, we have characterized Hef, which is involved in the stalled replication fork repair, Ume6, which is a transcription regulation factor, TRF1, which is a telomere binding factor, Nucleophsomin/B23, which is the viral chromatin regulator, and NAP1, which is the histone chaperone. All these proteins contain IDR. We analyzed each IDR for their binding to the various partner molecules and the general conclusion is that IDR have important functions for regulation of the interaction with their partner factors by occasional and sequential folding coupled with binding.