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Analysis of high CO2 response on the metabolic network consisting of C and N metabolic pathways with wild-type and genetically modified Arabidopsis plants

Planned Research

Project AreaComprehensive studies of plant responses to high CO2 world by an innovative consortium of ecologists and molecular biologists
Project/Area Number 21114004
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionThe University of Tokyo

Principal Investigator

YANAGISAWA Shuichi  東京大学, 生物生産工学研究センター, 准教授 (20222359)

Project Period (FY) 2009-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥111,930,000 (Direct Cost: ¥86,100,000、Indirect Cost: ¥25,830,000)
Fiscal Year 2013: ¥20,280,000 (Direct Cost: ¥15,600,000、Indirect Cost: ¥4,680,000)
Fiscal Year 2012: ¥20,280,000 (Direct Cost: ¥15,600,000、Indirect Cost: ¥4,680,000)
Fiscal Year 2011: ¥20,280,000 (Direct Cost: ¥15,600,000、Indirect Cost: ¥4,680,000)
Fiscal Year 2010: ¥20,280,000 (Direct Cost: ¥15,600,000、Indirect Cost: ¥4,680,000)
Fiscal Year 2009: ¥30,810,000 (Direct Cost: ¥23,700,000、Indirect Cost: ¥7,110,000)
Keywords高二酸化炭素 / 窒素同化 / メタボローム解析 / 植物 / 高CO2応答 / 代謝物 / 窒素 / 遺伝子発現 / 高CO_2応答 / 窒素応答 / C/Nバランス / C / Nバランス
Outline of Final Research Achievements

To investigate modulations in plant metabolism by the combined action of elevated atmospheric CO2 concentration and other nutritional factors, we performed targeted metabolomic analysis using Arabidopsis plants grown under 24 different conditions where the CO2 concentration, amounts and species of nitrogen source, and light intensity were modified. Our results indicate that both the biosynthesis of diverse metabolites and growth are promoted in proportion to the CO2 concentration at a wide range of CO2 level, although ammonium is a prominent factor determining metabolic status, while the CO2 concentration is not. Ammonium-induced metabolic alterations were differently modified by high concentrations of CO2, implying that increases in CO2 concentration may differently influence plant metabolism depending on the nitrogen nutrient conditions. We also performed targeted metabolomic analysis with Arabidopsis plants whose ability for nitrogen assimilation was genetically modified.

Report

(6 results)
  • 2014 Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (55 results)

All 2015 2014 2013 2012 2011 2010 2009 Other

All Journal Article (18 results) (of which Peer Reviewed: 18 results,  Open Access: 6 results,  Acknowledgement Compliant: 6 results) Presentation (34 results) (of which Invited: 5 results) Book (1 results) Remarks (2 results)

  • [Journal Article] Characterization of metabolic states of Arabidopsis thaliana under diverse carbon and nitrogen nutrient conditions via targeted metabolomic analysis2014

    • Author(s)
      Sato, S. and Yanagisawa, S.
    • Journal Title

      Plant Cell Physiology

      Volume: 55 Issue: 2 Pages: 306-319

    • DOI

      10.1093/pcp/pct192

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] High CO2 triggers preferential root growth of Arabidopsis thaliana via two distinct systems at low pH and low N stresses2014

    • Author(s)
      Hachiya, T., Sugiura, D., Kojima, M., Sato, S., Yanagisawa, S., Sakakibara, H., Terashima, I. and Noguchi, K.
    • Journal Title

      Plant Cell Physiology

      Volume: 55 Issue: 2 Pages: 269-280

    • DOI

      10.1093/pcp/pcu001

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Effects of elevated growth CO2 on the levels of primary metabolites and transcripts of respiratory enzymes and their diurnal patterns in Arabidopsis thaliana shoots: possible relations to the respiratory rates2014

    • Author(s)
      Watanabe, C.K., Sato, S., Yanagisawa, S., Uesono, Y., Terashima, I. and Noguchi, K.
    • Journal Title

      Plant Cell Physiology

      Volume: 55 Issue: 2 Pages: 341-357

    • DOI

      10.1093/pcp/pct185

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Nitrite transport activity of a novel HPP family protein conserved in cyanobacteria and chloroplasts2014

    • Author(s)
      Maeda, S., Konishi, M., Yanagisawa, S. and Omata, T.
    • Journal Title

      Plant Cell Physiology

      Volume: 55 Issue: 7 Pages: 1311-1324

    • DOI

      10.1093/pcp/pcu075

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Transcription factors involved in controlling the expression of nitrate reductase genes in higher plants2014

    • Author(s)
      Yanagisawa, S.
    • Journal Title

      Plant Science

      Volume: 229 Pages: 167-171

    • DOI

      10.1016/j.plantsci.2014.09.006

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Plant responses to CO2: Background and perspectives.2014

    • Author(s)
      Terashima I, Yanagisawa S, Sakakibara H
    • Journal Title

      Plant and Cell Physiology

      Volume: 54 Issue: 2 Pages: 237-240

    • DOI

      10.1093/pcp/pcu022

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Characterization of a nitrate-inducible transcriptional repressor NIGT1 provides new insights into DNA recognition by the GARP family proteins2013

    • Author(s)
      Yanagisawa, S.
    • Journal Title

      Plant Signaling & Behavior

      Volume: 8 Issue: 6 Pages: e24447-e24447

    • DOI

      10.4161/psb.24447

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quantification of zinc transport via the phloem to the grain in rice plants (Oryza sativa L.) at early grain-filling by a combination of mathematical modeling and 65Zn tracingy2013

    • Author(s)
      Nishiyama, R., Tanoi, K., Yanagisawa, S., Yoneyama, T.
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: 59 Issue: 5 Pages: 750-755

    • DOI

      10.1080/00380768.2013.819774

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The evolutionary events necessary for the emergence of symbiotic nitrogen fixation in legumes may involve a loss of nitrate responsiveness of the NIN transcription factor2013

    • Author(s)
      Suzuki, W., Konishi, M., Yanagisawa, S.
    • Journal Title

      Plant Signaling & Behavior

      Volume: 8 Issue: 10 Pages: e25975-e25975

    • DOI

      10.4161/psb.25975

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An NLP-binding site in the 3' flanking region of the nitrate reductase gene confers nitrate-inducible expression in Arabidopsis2013

    • Author(s)
      Konishi, M., Yanagisawa, S.
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: 59 Issue: 4 Pages: 612-620

    • DOI

      10.1080/00380768.2013.809602

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A nitrate-inducible GARP family gene encodes an auto-repressible transcriptional repressor in rice2013

    • Author(s)
      Sawaki, N., Tsujimoto, R., Shigyo, M., Konishi, M., Toki, S., Fujiwara, T. and Yanagisawa, S.
    • Journal Title

      Plant & Cell Physiology

      Volume: 54 Issue: 4 Pages: 506-517

    • DOI

      10.1093/pcp/pct007

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Arabidopsis NIN-like transcription factors play a central role in nitrate signalling2013

    • Author(s)
      Konishi, M. and Yanagisawa, S.
    • Journal Title

      Nature Communications

      Volume: 4 Issue: 1 Pages: 1617-1617

    • DOI

      10.1038/ncomms2621

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Dof Transcription Factor, SCAP1, Is Essential for the Development of Functional Stomata in Arabidopsis2013

    • Author(s)
      Negi, J., Moriwaki, K., Konishi, M., Yokoyama, R., Nakano, T., Kusumi, K., Hashimoto-Sugimoto, M., Schroeder, J.I., Nishitani, K., Yanagisawa, S. and Iba, K.
    • Journal Title

      Current Biology

      Volume: 23 Issue: 6 Pages: 479-484

    • DOI

      10.1016/j.cub.2013.02.001

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Proteomic characterization of the greening process in rice seedlings using the MS spectral intensity-based label free method2012

    • Author(s)
      Hamamoto, K., Aki, T., Shigyo, M., Sato, S., Ishida, T., Yano, K., Yoneyama, T., Yanagisawa, S.
    • Journal Title

      Journal of Proteome Research

      Volume: 11 Pages: 331-347

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Roles of the transcriptional regulation mediated by the nitrate-responsive cis-element in higher plants2012

    • Author(s)
      Mineko Konishi, Shuichi Yanagisawa
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 411 Pages: 708-713

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Regulatory Region Controlling the Nitrate-responsive Expression of a Nitrate Reductase Gene, NIA1, in Arabidopsis2011

    • Author(s)
      Mineko Konishi, Shuichi Yanagisawa
    • Journal Title

      Plant and Cell Physiology

      Volume: 52 Pages: 824-836

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of the nitrate-responsive cis-element in the Arabidopsis NIR1 promoter defines the presence of multiple cis-elements for nitrogen response2010

    • Author(s)
      Konishi, M., Yanagisawa, S.
    • Journal Title

      Plant J. (In press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of PpDof1 transcription factor in the nutrient-dependent growth control in Physcomitrella patens

    • Author(s)
      Sugiyama, T., Tabei, N., Ishida, T., Shigyo, M., Yoneyama, T., Yanagisawa, S.
    • Journal Title

      Journal of Experimental Botany

      Volume: (In press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 糖誘導性核小体局在タンパク質NuGAP1/APUM24のリボソーム生合成への関与の可能性2015

    • Author(s)
      前川修吾、石田哲也、柳澤修一
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2013 Annual Research Report
  • [Presentation] 胚発生と維管束パターン形成におけるシロイヌナズナDof5.8転写因子の役割2015

    • Author(s)
      小西美稲子、柳澤修一
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2013 Annual Research Report
  • [Presentation] 糖と窒素栄養応答に関与する核局在BTBタンパク質の機能解析2015

    • Author(s)
      佐々木勇樹, 安田盛貴, 柳澤修一,佐藤長緒, 山口淳二
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2013 Annual Research Report
  • [Presentation] 植物の窒素応答2015

    • Author(s)
      柳澤修一
    • Organizer
      第3回ブラキボディウムワークショップ
    • Place of Presentation
      川崎
    • Year and Date
      2015-03-05
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 胚発生に関与する糖誘導性核小体局在タンパク質APUM24/NuGAP1の機能解析2014

    • Author(s)
      前川修吾、石田哲也、柳澤修一
    • Organizer
      第32回日本植物細胞分子生物学会(盛岡)大会・シンポジウム
    • Place of Presentation
      盛岡
    • Year and Date
      2014-08-21 – 2014-08-22
    • Related Report
      2013 Annual Research Report
  • [Presentation] C/N栄養応答に関与する新規14-3-3タンパク質結合因子の機能解析2014

    • Author(s)
      佐々木勇樹、安田盛貴、柳澤修一、佐藤長緒、山口淳二
    • Organizer
      第32回日本植物細胞分子生物学会(盛岡)大会・シンポジウム
    • Place of Presentation
      盛岡
    • Year and Date
      2014-08-21 – 2014-08-22
    • Related Report
      2013 Annual Research Report
  • [Presentation] Sugar-inducible pumilio protein APUM24 is involved in both embryogenesis and leaf development in Arabidopsis2014

    • Author(s)
      前川修吾、石田哲也、柳澤修一
    • Organizer
      第16回日本RNA学会年会
    • Place of Presentation
      名古屋
    • Year and Date
      2014-07-23 – 2014-07-25
    • Related Report
      2013 Annual Research Report
  • [Presentation] 植物の硝酸応答機構研究の新展開2014

    • Author(s)
      柳澤修一,小西美稲子,鈴木渉
    • Organizer
      第55回日本植物生理学会年会シンポジウム「植物の三大栄養素(N-P-K)の感知と利用の新理解」
    • Place of Presentation
      富山
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] C/N栄養応答に関与する新規ユビキチンリガーゼBTタンパク質の機能解析2014

    • Author(s)
      佐々木勇樹, 安田盛貴, 柳澤修一,佐藤長緒, 山口淳二
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山
    • Related Report
      2012 Annual Research Report
  • [Presentation] 硝酸誘導型転写因子NLPによるBTタンパク質遺伝子の発現誘導2014

    • Author(s)
      吉岡希、小西美稲子、石田哲也、柳澤修一
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山
    • Related Report
      2012 Annual Research Report
  • [Presentation] 石田哲也、柳澤修一2014

    • Author(s)
      グルコース応答性遺伝子AtNuGAP1由来の核タンパク質の胚発生への関与
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山
    • Related Report
      2012 Annual Research Report
  • [Presentation] 高等植物の硝酸応答を担うNLP転写因子の機能ドメインの解析2014

    • Author(s)
      小西美稲子、鈴木渉、柳澤修一
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山
    • Related Report
      2012 Annual Research Report
  • [Presentation] Members of the nin-like protein family are transcription factors governing nitrate-inducible gene expression2013

    • Author(s)
      Yanagisawa, S.
    • Organizer
      Nitrogen 2013 (2nd International Symposium on the Nitrogen Nutrition of Plants
    • Place of Presentation
      Puerto Varas, Chile
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] The central role of NLP transcription factors in nitrate-inducible gene expression2013

    • Author(s)
      Mineko Konishi, Shuichi Yanagisawa
    • Organizer
      The 24th International Conference on Arabidopsis Research (ICAR)
    • Place of Presentation
      Sydney, Australia
    • Related Report
      2012 Annual Research Report
  • [Presentation] つくばFACEサイトのイネの葉の呼吸系の高CO2応答2013

    • Author(s)
      野口航,渡辺千尋,酒井英光,長谷川利拡,柳澤修一,寺島一郎
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      札幌
    • Related Report
      2012 Annual Research Report
  • [Presentation] Transcriptional regulation of gibberellin biosynthesis by ethylene signaling in the growth response of submerged deepwater rice (tkuroha@agr.nagoya-u.ac.jp)2013

    • Author(s)
      Kuroha, T., Keisuke Nagai, K., Ayano, M., Yanagisawa, S., Ashikari, M.
    • Organizer
      The 21st International Conference on Plant Growth Substances IPGSA2013
    • Place of Presentation
      Shanghai, China
    • Related Report
      2012 Annual Research Report
  • [Presentation] NIN-like proteinによる硝酸誘導性遺伝子発現の制御2013

    • Author(s)
      小西美稲子、鈴木渉、柳澤修一
    • Organizer
      日本土壌肥料学会2013 年度名古屋大会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Annual Research Report
  • [Presentation] シロイヌナズナの窒素応答2012

    • Author(s)
      柳澤修一
    • Organizer
      日本学術振興会産学協力第160委員会第2回研究会「植物栄養代謝バランスの多方面からの理解」
    • Place of Presentation
      福岡
    • Year and Date
      2012-03-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] シロイヌナズナの環境栄養応答に関する主要代謝経路のメタボローム測定データを用いた比較ネットワーク解析2012

    • Author(s)
      佐藤滋、柳澤修一
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都
    • Related Report
      2011 Annual Research Report
  • [Presentation] Molecular Mechanism Underlying Nitrate-responsive Gene Expression in Arabidopsis2012

    • Author(s)
      Yanagisawa, S. and Konishi, K.
    • Organizer
      22nd IUBMB - 37th FEBS Congress
    • Place of Presentation
      スペイン、セビリア
    • Related Report
      2011 Annual Research Report
  • [Presentation] 高等植物の硝酸応答機構2011

    • Author(s)
      小西美稲子、柳澤修一
    • Organizer
      日本植物学会第75回大会シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2011-09-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] 植物バイオマスと窒素同化2011

    • Author(s)
      柳澤修一
    • Organizer
      日本植物細分子生物学会2011年度大会・シンポジウム「低炭素社会に向けた植物バイオマス研究の新展開」
    • Place of Presentation
      福岡(九州大学)
    • Related Report
      2010 Annual Research Report
  • [Presentation] シロイヌナズナ高CO_2応答のメタボローム解析2011

    • Author(s)
      柳澤修一
    • Organizer
      第84回日本生化学会大会シンポジウム「高CO_2な近未来環境で植物はどうなるか?-その理解のための生化学的アプローチ-」
    • Place of Presentation
      京都
    • Related Report
      2010 Annual Research Report
  • [Presentation] 高等植物における窒素応答型遺伝子発現の分子機構2010

    • Author(s)
      柳澤修一
    • Organizer
      作物学会 ミニシンポジウム
    • Place of Presentation
      宇都宮
    • Year and Date
      2010-03-31
    • Related Report
      2009 Annual Research Report
  • [Presentation] シロイヌナズナ硝酸還元酵素遺伝子NIA1の発現制御機構の解析2010

    • Author(s)
      小西美稲子、柳澤修一
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      熊本
    • Year and Date
      2010-03-21
    • Related Report
      2009 Annual Research Report
  • [Presentation] 植物の翻訳開始因子eIF6の機能解析2010

    • Author(s)
      加藤裕樹、小西美稲子、米山忠克、柳澤修一
    • Organizer
      日本植物生理学会シンポジウム
    • Place of Presentation
      熊本
    • Year and Date
      2010-03-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] ナノスケールでの植物プロテオミクス:新規シグナル応答因子の同定に向けた3つの応用例2010

    • Author(s)
      秋利彦、柳澤修一
    • Organizer
      日本植物生理学会シンポジウム
    • Place of Presentation
      熊本
    • Year and Date
      2010-03-18
    • Related Report
      2009 Annual Research Report
  • [Presentation] CE-MSによる代謝プロファイリング:陰イオン性代謝物質測定のための2つの分離モード2010

    • Author(s)
      佐藤滋、柳澤修一
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      熊本
    • Year and Date
      2010-03-18
    • Related Report
      2009 Annual Research Report
  • [Presentation] 進化的に保存された糖誘導型核タンパク質の同定と解析2010

    • Author(s)
      石田哲也、秋利彦、柳澤修一
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      熊本
    • Year and Date
      2010-03-18
    • Related Report
      2009 Annual Research Report
  • [Presentation] An attempt to improve nitrogen utilization efficiency of crops : genetic modification with the Dof1 transcription factor2010

    • Author(s)
      柳澤修一
    • Organizer
      Plant and Animal Genome XVIII Conference
    • Place of Presentation
      San Diego, California, USA
    • Year and Date
      2010-01-10
    • Related Report
      2009 Annual Research Report
  • [Presentation] Involvement of Dof transcription factors from the moss, Physcomitrella patens, in growth regulation2009

    • Author(s)
      Takumi Sugiyama, Nobumitsu Tabei, Mikao Shigyo, Tadakatsu Yoneyama, Shuichi Yanagisawa
    • Organizer
      日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-11
    • Related Report
      2009 Annual Research Report
  • [Presentation] 植物転写因子を用いた植物機能の強化

    • Author(s)
      柳澤修一
    • Organizer
      生物生産工学研究センターシンポジウム "植物機能のバイオテ クノロジー"
    • Place of Presentation
      東京
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] イネ緑化過程の比較プロテオーム解析

    • Author(s)
      柳澤修一
    • Organizer
      日本プロテオーム学会2012年大会、日本ヒトプトテオーム機構 第10回大会シンポジウム "農学プロテオーム研究の最前線"
    • Place of Presentation
      東京
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] NBP転写因子による硝酸応答の制御

    • Author(s)
      小西美稲子、柳澤修一
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山
    • Related Report
      2011 Annual Research Report
  • [Book] Transcription factors : improving abiotic stress tolerance in plants, in Improving Stress Resistance to Abiotic Stress(Narendra Tuteja, ed.)2012

    • Author(s)
      Ishida, T., Osakabe, Y., Yanagisawa, S.
    • Total Pages
      30
    • Publisher
      Wiley-Blackwell, Wiley-VCH Verlag GmbH & Co.(Germany)
    • Related Report
      2010 Annual Research Report
  • [Remarks] 窒素栄養の利用を制御する植物の仕組み

    • URL

      http://www.u-tokyo.ac.jp/ja/utokyo-research/tag/nin-like-transcription-factor/

    • Related Report
      2012 Annual Research Report
  • [Remarks] NIN様転写因子が植物の硝酸応答を司る

    • URL

      http://www.a.u-tokyo.ac.jp/topics/2013/20130321-1.html

    • Related Report
      2012 Annual Research Report

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Published: 2009-04-01   Modified: 2019-07-29  

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