| Budget Amount *help |
¥153,660,000 (Direct Cost: ¥118,200,000、Indirect Cost: ¥35,460,000)
Fiscal Year 2013: ¥31,070,000 (Direct Cost: ¥23,900,000、Indirect Cost: ¥7,170,000)
Fiscal Year 2012: ¥31,070,000 (Direct Cost: ¥23,900,000、Indirect Cost: ¥7,170,000)
Fiscal Year 2011: ¥31,070,000 (Direct Cost: ¥23,900,000、Indirect Cost: ¥7,170,000)
Fiscal Year 2010: ¥31,070,000 (Direct Cost: ¥23,900,000、Indirect Cost: ¥7,170,000)
Fiscal Year 2009: ¥29,380,000 (Direct Cost: ¥22,600,000、Indirect Cost: ¥6,780,000)
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| Research Abstract |
At the core of the small RNA effector complex lie Argonaute family proteins, which can be divided into ubiquitous AGO subfamily and gonad-specific PIWI subfamily. We have made a series of important discoveries about the effector complex assembly and target silencing functions of AGO-clade small RNAs, including our finding that the N domain of AGO plays a critical role in separating the two small RNA strands within AGO to mature the effector complex. We have also successfully reconstituted the core part of the piRNA biogenesis pathway in vitro, paving a new path to biochemical understanding of PIWI-clade small RNAs.
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