|Budget Amount *help
¥96,460,000 (Direct Cost : ¥74,200,000、Indirect Cost : ¥22,260,000)
Fiscal Year 2013 : ¥17,810,000 (Direct Cost : ¥13,700,000、Indirect Cost : ¥4,110,000)
Fiscal Year 2012 : ¥17,810,000 (Direct Cost : ¥13,700,000、Indirect Cost : ¥4,110,000)
Fiscal Year 2011 : ¥18,980,000 (Direct Cost : ¥14,600,000、Indirect Cost : ¥4,380,000)
Fiscal Year 2010 : ¥17,940,000 (Direct Cost : ¥13,800,000、Indirect Cost : ¥4,140,000)
Fiscal Year 2009 : ¥23,920,000 (Direct Cost : ¥18,400,000、Indirect Cost : ¥5,520,000)
In this mission, we attempted to figure out the regulatory mechanisms of Xist RNA machinery, which has been known for many years to play a pivotal role in X chromosome inactivation in female mammals. Especially, we focused on the potential role of the proteins, which has been shown to localize to the inactive X, and their interacting partners in Xist RNA-mediated chromatin silencing. The findings indicates the importance of further studies of the potential role of factors involved in replication programming in the Xist RNA machinery. In addition, we have paved the way to our further understanding of the Xist RNA machinery by deriving various cell lines from the mice we established by knocking out a protein involved in the regulation of Xist RNA deposition to the inactive X.