Studies on the roles of small RNA in mice

• Project Area: Functional machinery for non-coding RNAs
• Project/Area Number: 21115006
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Osaka University (2013); The University of Tokyo (2009-2012)
• Principal Investigator: NAKAZAWA Takanobu 大阪大学, 薬学研究科(研究院), 特任准教授 (00447335)
• Co-Investigator(Kenkyū-buntansha): MIYAGAWA Satomi 大阪大学, 大学院医学系研究科, 特任講師 (90291153)
• Project Period (FY): 2009-07-23 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥120,250,000 (Direct Cost: ¥92,500,000、Indirect Cost: ¥27,750,000); Fiscal Year 2013: ¥25,350,000 (Direct Cost: ¥19,500,000、Indirect Cost: ¥5,850,000); Fiscal Year 2012: ¥25,220,000 (Direct Cost: ¥19,400,000、Indirect Cost: ¥5,820,000); Fiscal Year 2011: ¥22,230,000 (Direct Cost: ¥17,100,000、Indirect Cost: ¥5,130,000); Fiscal Year 2010: ¥21,840,000 (Direct Cost: ¥16,800,000、Indirect Cost: ¥5,040,000); Fiscal Year 2009: ¥25,610,000 (Direct Cost: ¥19,700,000、Indirect Cost: ¥5,910,000)
• Keywords: 小分子RNA / miRNA / piRNA / non-coding RNA / 中枢神経系 / 精巣 / 神経細胞 / 精子形成 / 脳・神経 / マウス / 神経細胞形態制御

Research Abstract

In this study, we showed the importance of small RNAs in brain and testis as described below. 1) We found that several miRNAs in the striatum are involved in cocaine addiction. 2) Using unbiased screening methods, we found that several miRNAs, such as miR-132, regulates neural morphogenesis. 3) To analyze the molecular function of MILI in piRNA biogenesis, we utilized germline stem (GS) cells, which are derived from testicular stem cells and possess a spermatogonial phenotype. We found that glycerol-3-phosphate acyltransferase 2 (GPAT2), a mitochondrial outer membrane protein for lysophosphatidic acid, bounds to MILI in GS cells. 4) We developed a novel artificial piRNA production system to reveal that the concomitant expression of sense and antisense RNA transcripts is necessary and sufficient for piRNA production and subsequent piRNA-dependent gene silencing.

Research Products

• [Journal Article] Retrograde Semaphorin Signaling Regulates Synapse Elimination in the Developing Mouse Brain (2014)
  • Author(s): Uesaka N, Uchigashima M, Mikuni T,Nakazawa T, Nakao H, Hirai H, Aiba A,Watanabe M, Kano M.
  • Journal Title: Science Volume: 344(PMID:24831527) Pages: 1020-1023
• [Journal Article] LMTK3 Deficiency Causes Pronounced Locomotor Hyperactivity and Impairs Endocytic Trafficking (2014)
  • Author(s): Inoue T, Hoshina N, Nakazawa T, Kiyama Y, Kobayashi S, Abe T, Yamamoto T, Manabe T, Yamamoto T.
  • Journal Title: J Neurosci Volume: 34(17) Issue: 17 Pages: 5927-5937
  • DOI: 10.1523/jneurosci.1621-13.2014
  • Peer Reviewed / Open Access
• [Journal Article] Analysis of DNA methylation in Mouse Testis (2014)
  • Author(s): Kuramochi-Miyagawa S, Kojima-Kita K, Shiromoto Y, Itou D, Koshima H, Nakano T
  • Journal Title: Methods in Mol. Biol Volume: 1093 Pages: 97-109
  • DOI: 10.1007/978-1-62703-694-8_8
  • ISBN: 9781627036931, 9781627036948
  • Peer Reviewed / Open Access
• [Journal Article] Increased Behavioral and Neuronal Responses to a Hallucinogenic Drug in PACAP Heterozygous Mutant Mice. (2014)
  • Author(s): Keisuke Hazama
  • Journal Title: PLoS ONE Volume: 20 Issue: 2 Pages: 89153-89153
  • DOI: 10.1371/journal.pone.0089153
  • Peer Reviewed / Open Access
• [Journal Article] Central CRTH2, a second prostaglandin D2 receptor, mediates emotional impairment in the lipopolysaccharide and tumor-induced sickness behavior model. (2014)
  • Author(s): Haba R, Shintani N, Onaka Y, Kanoh T, Wang H, Takenaga R, Hayata A, Hirai H, Nagata KY, Nakamura M, Kasai A, Haghi moto R, Nagayasu K, Nakazawa T, Hashimoto H, Baba A.
  • Journal Title: J Neurosci. Volume: 34 Issue: 7 Pages: 2514-2523
  • DOI: 10.1523/jneurosci.1407-13.2014
  • Peer Reviewed / Open Access
• [Journal Article] Protocadherin 17 regulates presynaptic assembly in topographic corticobasal ganglia circuits (2013)
  • Author(s): Hoshina N, Tanimura A, Yamasaki M, Inoue T, Fukabori R, Kuroda T, Yokoyama K, Tezuka T, Sagara H, Hirano S, Kiyonari H, Takada M, Kobayashi K, Watanabe M, Kano M, Nakazawa T, Yamamoto T
  • Journal Title: Neuron Volume: 78 Issue: 5 Pages: 839
  • DOI: 10.1016/j.neuron.2013.03.031
  • URL: https://localhost/en/publications/9a3f8593-f1a7-4ad9-9e1b-b33363b03e50
  • Peer Reviewed
• [Journal Article] The nuage mediates retrotransposon silencing in mouse primordial ovarian follicles (2013)
  • Author(s): Lim AK, Lorthongpanich C, Chew TG, Tan CW, Shue YT, Balu S, Gounko N, Kuramochi-Miyagawa S, Matzuk MM, Chuma S, Messerschmidt DM, Solter D, Knowles BB
  • Journal Title: Development Volume: 140(18) Issue: 18 Pages: 3819-3825
  • DOI: 10.1242/dev.099184
  • Peer Reviewed
• [Journal Article] Targeted gene silencing in mouse germ cells by insertion of a homologous DNA into a piRNA generating locus (2013)
  • Author(s): Yamamoto Y,Watanabe T,Hoki Y, Shirane K, Li Y, Ichiiyanagi K, Kuramochi-Miyagawa S, Toyoda A, Fujiyama A, Oginuma M, Suzuki H, Sado T, Nakano T, Sasaki H
  • Journal Title: Genome Res Volume: 23 Issue: 2 Pages: 292-299
  • DOI: 10.1101/gr.137224.112
  • Peer Reviewed
• [Journal Article] GPAT2, a Mitochondrial Outer Membrane Protein in piRNA Biogenesis in Germline Stem Cells (2013)
  • Author(s): Shiromoto Y, Kuramochi-Miyagawa S, Daiba A, Chuma S, Katanaya A, Nishimura K, Ohtaka M, Nakanishi M, Nakamura T, Yoshinaga K, Asada N, Nakamura S, Yasunaga T, Kojima-Kita K, Itou D, Kimura T, Nakano T
  • Journal Title: RNA Volume: 19 Issue: 6 Pages: 803-810
  • DOI: 10.1261/rna.038521.113
  • Peer Reviewed
• [Journal Article] Role of protocadherin 17 in presynaptic assembly of the specific cortico-basal ganglia circuit neurons. (2013)
  • Author(s): Hoshina N
  • Journal Title: Neuron Volume: in press
  • Peer Reviewed
• [Journal Article] GPAT2, a Mitochondrial Outer Membrane Protein, in piRNA Biogenesis in Germline Stem Cell. (2013)
  • Author(s): Shiromoto Y
  • Journal Title: RNA Volume: in press
  • Peer Reviewed
• [Journal Article] Metaplasticity gated through differential regulation of GluN2A versus GluN2B receptors by Src family kinases (2012)
  • Author(s): Yang K, Trepanier C, Sidhu B, Xie YF, Li H, Lei G, Salter MW, Orser BA, Nakazawa T, Yamamoto T, Jackson MF, Macdonald JF
  • Journal Title: EMBO J Volume: 31(4) Issue: 4 Pages: 805-816
  • DOI: 10.1038/emboj.2011.453
  • Peer Reviewed
• [Journal Article] The p250GAP gene is associated with risk for schizophrenia and schizotypal personality traits (2012)
  • Author(s): Hashimoto R
  • Journal Title: PLoS ONE Volume: 7 Issue: 4 Pages: e35696-e35696
  • DOI: 10.1371/journal.pone.0035696
  • URL: https://pure.teikyo.jp/en/publications/4db5e609-1ad4-45ab-8119-8a26d97d88c8
  • Peer Reviewed
• [Journal Article] NMDAR2B tyrosine phosphorylation is involved in thermal nociception (2012)
  • Author(s): Delawary M, Tezuka T, Yamamoto T. (9名)
  • Journal Title: Neurosci Lett Volume: Vol.516 Issue: 2 Pages: 270-273
  • DOI: 10.1016/j.neulet.2012.04.007
  • Peer Reviewed
• [Journal Article] Involvement of Tyr1472 phosphorylation of NMDA receptor NR2B subunit in postherpetic neuralgia in model mice (2012)
  • Author(s): Unezaki S, Sasaki A, Yamamoto T. (12名)
  • Journal Title: Mol Pain Volume: Vol.8 Pages: 8-8
  • DOI: 10.1186/1744-8069-8-59
  • Peer Reviewed
• [Journal Article] p250GAP is a novel player in the Cdh1-APC/Smurf1 pathway of axon growth regulation. (2012)
  • Author(s): Kannan M
  • Journal Title: PLoS One Volume: 7(11) Issue: 11 Pages: e50735-e50735
  • DOI: 10.1371/journal.pone.0050735
  • Peer Reviewed
• [Journal Article] Identification of MIWI-associated Poly(A) RNAs by immunoprecipitation with an anti-MIWI monoclonal antibody (2012)
  • Author(s): Nishibu, T., Hayashida, Y., Tani, S., Kurono, S., Kojima-Kita, K., Ukekawa, R., Kurokawa, T., Kuramochi-Miyagawa, S., Nakano, T., Inoue, K., and Honda, S.
  • Journal Title: BioScience Trends Volume: 6 Issue: 5 Pages: 248-261
  • DOI: 10.5582/bst.2012.v6.5.248
  • NAID: 130005054634
  • ISSN: 1881-7815, 1881-7823
  • Peer Reviewed
• [Journal Article] NYAP : a phosphoprotein family that links PI3K to WAVE1 signalling in neurons (2011)
  • Author(s): K.Yokoyama
  • Journal Title: EMBO J Volume: 30 Issue: 23 Pages: 4739-4754
  • DOI: 10.1038/emboj.2011.348
  • Peer Reviewed
• [Journal Article] Locus- and domain-dependent control of DNA methylation at mouse B1 retrotransposons during male germ cell development (2011)
  • Author(s): Ichiyanagi, T., Fukuda, K., Kitayama, J., Yamamoto, Y., Kuramochi-Miyagawa, S., Nakano, T., Yabuta, Y., Seki, Y., Saitou, M., & Sasaki, H
  • Journal Title: Genome Res Volume: 21 Issue: 12 Pages: 2058-2066
  • DOI: 10.1101/gr.123679.111
  • Peer Reviewed
• [Journal Article] Tudor domain containing 7 (Tdrd7) is essential for dynamic ribonucleoprotein (RNP) remodeling of chromatoid bodies during spermatogenesis (2011)
  • Author(s): Tanaka T, et al
  • Journal Title: Proc Natl Acad Sci USA Volume: 108 Issue: 26 Pages: 10579-10584
  • DOI: 10.1073/pnas.1015447108
  • Peer Reviewed
• [Journal Article] Role for piRNAs and non-coding RNA in de novo DNA methylation of the imprinted mouse Rasgrf1 locus (2011)
  • Author(s): Watanabe, T., Tomizawa, S., Mitsuya, K., Totoki, Y., Yamamoto, Y., Kuramochi-Miyagawa, S., Iida, N., Hoki, Y., Murphy, P.J., Toyoda, A., Gotoh, K., Hiura, H., Arima, T., Fujiyama, A., Sado, T., Shibata, T., Nakano, T., Lin, H., Ichiyanagi, K., Soloway, P.D., Sasaki, H
  • Journal Title: Science Volume: 332 Issue: 6031 Pages: 848-52
  • DOI: 10.1126/science.1203919
  • Peer Reviewed
• [Journal Article] MitoPLD Is a Mitochondrial Protein Essential for Nuage Formation and piRNA Biogenesis in the Mouse Germline (2011)
  • Author(s): Watanabe T, et al
  • Journal Title: Dev Cell Volume: 20 Issue: 3 Pages: 364-375
  • DOI: 10.1016/j.devcel.2011.01.005
  • Peer Reviewed
• [Journal Article] Distinct expression patterns of the subunits of the CCR4-NOT deadenylase complex during neural development (2011)
  • Author(s): Chen C, et al
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 411 Issue: 2 Pages: 360-364
  • DOI: 10.1016/j.bbrc.2011.06.148
  • Peer Reviewed
• [Journal Article] Involvement of spinal phosphorylation cascade of Tyr1472-NR2B, Thr286-CaMKII, and Ser831-GluR1 in neuropathic pain (2011)
  • Author(s): Katano T, et al
  • Journal Title: Neuropharmacology Volume: 60 Pages: 609-616
  • Peer Reviewed
• [Journal Article] MVH in piRNA processing and gene silencing of retrotransposons (2010)
  • Author(s): Kuramochi-Miyagawa, S., Watanabe, T., Gotoh, K., Takamatsu, K., Chuma, S., Kojima-Kita, K., Shiromoto, Y., Asada, N., Kimura, T., Nakatsuji, N., Noce, T., Sasaki, H. & Nakano, T
  • Journal Title: Genes Dev Volume: 24 Issue: 9 Pages: 887-892
  • DOI: 10.1101/gad.1902110
  • Peer Reviewed
• [Journal Article] Receptor tyrosine phosphatase sigma (RPTPsigma) regulates, p250GAP, a novel substrate that attenuates Rac signaling (2010)
  • Author(s): Chagnon MJ, et al
  • Journal Title: Cellular Signaling Volume: 22 Pages: 1626-1633
  • Peer Reviewed
• [Journal Article] Impairment of CaMKII activation and neuropathic pain in mice lacking NR2B phosphorylation at Tyr-1472 (2010)
  • Author(s): Matsumura S, et al
  • Journal Title: European Journal of Neuroscience Volume: 32 Pages: 798-810
  • Peer Reviewed
• [Journal Article] NMDAR2B tyrosine phosphorylation regulates anxiety-like behavior and CRF expression in the amygdala (2010)
  • Author(s): Delawary M, et al
  • Journal Title: Molecular Brain Volume: 3 Pages: 37-37
  • Peer Reviewed
• [Journal Article] MVH in piRNA Processing and Gene Silencing of Retrotransposons (2010)
  • Author(s): Kuramochi-Miyagawa S., et.al.
  • Journal Title: Genes and Development Volume: 23 Pages: 887-892
  • Peer Reviewed
• [Journal Article] Involvement of NMDAR2A tyrosine phosphorylation in depression-related behaviour (2009)
  • Author(s): Taniguchi S, Nakazawa T, Tanimura A, Kiyama Y, Tezuka T, Watabe AM, Katayama N, Yokoyama K, Inoue T, Izumi-Nakaseko H, Kakuta S, Sudo K, Iwakura Y, Umemori H, Inoue T, Murphy NP, Hashimoto K, Kano M, Manabe T, Yamamoto T
  • Journal Title: EMBO J Volume: 28 Issue: 23 Pages: 3717
  • DOI: 10.1038/emboj.2009.300
  • URL: https://pure.teikyo.jp/en/publications/273bda01-2279-440a-9fca-7681eb6af05c
  • Peer Reviewed
• [Journal Article] Involvement of NMDAR2A tyrosine phosphorylation in Depression-related behavior (2009)
  • Author(s): Taniguchi, Nakazawa, Tanimura, et al.
  • Journal Title: The EMBO Journal 28 Pages: 3717-3729
  • Peer Reviewed
• [Journal Article] Associations between PIWI proteins and TDRD1/MTR-1 are critical for integrated subcellular localization in murine male germ cells (2009)
  • Author(s): Kojima K, et al.
  • Journal Title: Genes to Cells 14 Pages: 1155-1165
  • Peer Reviewed
• [Journal Article] The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencine in the mouse male germline (2009)
  • Author(s): Siomi, Kuramochi-Miyagawa
  • Journal Title: Current Opinion in Cell Biology 21 Pages: 426-434
• [Journal Article] The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mouse male germline (2009)
  • Author(s): Shoji, et al.
  • Journal Title: Developmental Cell 17 Pages: 775-787
  • Peer Reviewed
• [Journal Article] A broadly conserved pathway generates 3'UTR-directed primary piRNAs (2009)
  • Author(s): Robine, et al.
  • Journal Title: Current Biology 19 Pages: 2066-2076
  • Peer Reviewed
• [Journal Article] Gtsf1/Cue110, a gene encoding a protein with two copies of a CHHC Zn-finger motif, is involved in spermatogenesis and retrotransposon suppression in murine testes (2009)
  • Author(s): Yoshimura, et al.
  • Journal Title: Developmental Biology 335 Pages: 216-227
  • Peer Reviewed
• [Presentation] 雄性生殖細胞におけるpiRNA依存的サイレンシングの人為的誘導 (2014)
  • Author(s): 伊藤大介、宮川（倉持）さとみ、仲野徹
  • Organizer: 新学術領域『非コードRNA作用マシナリー』領域班会議
  • Place of Presentation: グランドエクシブ那須白河（福島県西白河郡）
• [Presentation] 神経細胞の形態形成と統合失調症 (2013)
  • Author(s): 中澤敬信
  • Organizer: Neuro2013
  • Place of Presentation: 国立京都国際会館(京都府京都市)
• [Presentation] MVH ヘリカーゼ活性はLINE-1 レトロトランスポゾンの抑制に必須である (2013)
  • Author(s): 伊藤大介、宮川さとみ、仲野徹
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場(兵庫県神戸市)
• [Presentation] An autism-linked mutation in neuroligin-3 affects development of climbing fiber to Purkinje cell synapse in the cerebellum. (2013)
  • Author(s): Lai ESK, Nakayama H, Nakazawa T, Tabuchi K, Hashimoto K, Kano M
  • Organizer: 第36回日本神経科学学会大会（Neuro2013）
  • Place of Presentation: 国立京都国際会館（京都府京都市）
• [Presentation] 神経細胞の形態形成と統合失調症 (2013)
  • Author(s): 中澤敬信
  • Organizer: 第36回日本神経科学学会大会（Neuro2013）
  • Place of Presentation: 国立京都国際会館（京都府京都市）
  • Invited
• [Presentation] 精神疾患マウスモデルを用いた発症脆弱性因子および創薬標的分子の探索 (2013)
  • Author(s): 橋本均、新谷紀人、早田敦子、笠井淳司、永安一樹、中澤敬信
  • Organizer: 第23回日本臨床精神神経薬理学会・第43回日本神経精神薬理学会 合同年会
  • Place of Presentation: 沖縄コンベンションセンター(沖縄県宜野湾市）
• [Presentation] 人為的piRNA産生を介したDNAメチル化の操作 (2013)
  • Author(s): 伊藤大介、宮川（倉持）さとみ、城本悠助、仲野徹
  • Organizer: 第7回日本エピジェネティクス研究会年会
  • Place of Presentation: 奈良県新公会堂（奈良県奈良市）
• [Presentation] ミトコンドリア外膜タンパクGPAT2のpiRNA産生における機能 (2013)
  • Author(s): 城本悠助、宮川（倉持）さとみ、仲野徹
  • Organizer: 第36回日本分子生物学会
  • Place of Presentation: 神戸国際会議場（兵庫県神戸市）
• [Presentation] MVHヘリカーゼ活性はLINE-1レトロトランスポゾンの抑制に必須である (2013)
  • Author(s): 伊藤大介、宮川（倉持）さとみ、仲野徹
  • Organizer: 第36回日本分子生物学会
  • Place of Presentation: 神戸国際会議場（兵庫県神戸市）
• [Presentation] 雄性生殖細胞におけるpiRNA依存的遺伝子サイレンシングの人為的誘導 (2013)
  • Author(s): 新屋幸穂、伊藤大介、宮川（倉持）さとみ、仲野徹
  • Organizer: 第36回日本分子生物学会
  • Place of Presentation: 神戸国際会議場（兵庫県神戸市）
• [Presentation] プロテオミクス解析による神経障害性疼痛機能性分子の探索 (2012)
  • Author(s): 片野泰代
  • Organizer: 第85回 日本生化学会・大会
  • Place of Presentation: 福岡市 福岡国際会議場
  • Year and Date: 2012-12-15
• [Presentation] マウス精巣における人為的piRNA産生を介したDNAメチル化制御 (2012)
  • Author(s): 伊藤大介
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡市 福岡国際会議場・マリンメッセ福岡
  • Year and Date: 2012-12-14
• [Presentation] Search of neuropathic pain related proteins in neuropathic pain model mice by iTRAQ proteome analysis (2012)
  • Author(s): Katano T
  • Organizer: 42th ANNUAL MEETING Society for Neuroscience
  • Place of Presentation: New Orleans USA
  • Year and Date: 2012-11-16
• [Presentation] The p250GAP gene is associated with risk for schizophrenia and schizotypal personality trait (2012)
  • Author(s): 大井一高
  • Organizer: 第22回日本臨床精神神経薬理学会・第42回日本神経精神薬理学会合同年会
  • Place of Presentation: 宇都宮市 栃木県総合文化センター
  • Year and Date: 2012-10-19
• [Presentation] Association between the p250GAP/TCGAP family of RhoGAP genes and schizophrenia (2012)
  • Author(s): 中澤敬信
  • Organizer: 第35回日本神経科学会大会
  • Place of Presentation: 名古屋国際会議場(愛知県名古屋市)
  • Year and Date: 2012-09-19
• [Presentation] Association between the p250GAP/TCGAP family of RhoGAP genes and schizophrenia (2012)
  • Author(s): 中澤敬信
  • Organizer: 第35回日本神経科学会大会
  • Place of Presentation: 名古屋市 名古屋国際会議場
  • Year and Date: 2012-09-19
  • Invited
• [Presentation] De novo DNA methylation by artificially produced piRNA in murine testes (2012)
  • Author(s): Itou D
  • Organizer: Epigenetics and Chromatin in Cold Spring Habor Laboratory meeting
  • Place of Presentation: New York USA
  • Year and Date: 2012-09-12
• [Presentation] PAT2, a mitochondrial acyltransferase, in piRNA biogenesis in germline stem cells (2012)
  • Author(s): Shiromoto Y, Kuramochi-Miyagawa S, Daiba A, Chuma S, Nishimura K, Nakano T
  • Organizer: REGULATORY & NON-CODING RNAs in Cold Spring Habor Laboratory meeting
  • Place of Presentation: Cold Spring Habor Laboratory, New York, USA
  • Year and Date: 2012-08-30
• [Presentation] GPAT2, a mitochondrial acyltransferase, in piRNA biogenesis in germline stem cells. (2012)
  • Author(s): Shiromoto Y
  • Organizer: REGULATORY NON-CODING RNAs in Cold Spring Habor Laboratory meeting
  • Place of Presentation: New York USA
  • Year and Date: 2012-08-30
• [Presentation] 精子幹細胞を用いたpiRNA産生機構の解析 (2012)
  • Author(s): 城本悠助
  • Organizer: 特定領域生殖サイクル若手勉強会 2012
  • Place of Presentation: 仙台市 秋保リゾート ホテルクレセント
  • Year and Date: 2012-07-25
• [Presentation] p250GAP/ARHGAP32 regulates spine morphogenesis and is associated with risk for schizophrenia (2012)
  • Author(s): Nakazawa T
  • Organizer: 8th Forum of European Neuroscience (FENS)
  • Place of Presentation: Barcelona Spain
  • Year and Date: 2012-07-14
• [Presentation] The p250GAP gene is associated with risk for schizophrenia and schizotypal personality trait (2012)
  • Author(s): Hashimoto R
  • Organizer: 28th The International College of Neuro-Psychopharmacology (CINP) Congress
  • Place of Presentation: Stockholm Sweden
  • Year and Date: 2012-06-03
• [Presentation] 精子幹細胞を用いたpiRNA産生機構の解析 (2012)
  • Author(s): 城本悠助
  • Organizer: NGS現場の会 第二回研究会
  • Place of Presentation: 大阪府吹田市 ホテル阪急エキスポパーク
  • Year and Date: 2012-05-24
• [Presentation] マウス精巣におけるpiRNAを介した配列特異的なDNAメチル化誘導システム (2012)
  • Author(s): 伊藤大介
  • Organizer: 第6回 日本エピジェネティクス研究会年会
  • Place of Presentation: 東京都 一ツ橋学術総合センター
  • Year and Date: 2012-05-15
• [Presentation] DNA methylation of retrotransposon genes is important for meiotic synapsis in spermatogenesis (2011)
  • Author(s): Satomi Kuramochi-Miyagawa
  • Organizer: 第34回日本分子生物学会・年会
  • Place of Presentation: パシフィコ横浜(神奈川県横浜市)
  • Year and Date: 2011-12-16
• [Presentation] A role of protocadherin 17 in presynaptic assembly of cortico-basal ganglia circuit neurons (2011)
  • Author(s): Hoshina N, et al
  • Organizer: 第33回日本分子生物学会・年会
  • Place of Presentation: パシフィコ横浜(神奈川県横浜市)
  • Year and Date: 2011-12-13
• [Presentation] 精子形成における小分子RNA産生のしくみと役割 (2011)
  • Author(s): Satomi Kuramochi-Miyagawa
  • Organizer: 第3回「RNAと生体機能」さきがけ研究報告会
  • Place of Presentation: 東京大学弥生講堂(東京都文京区)
  • Year and Date: 2011-12-12
• [Presentation] The activation of NR2B-NMDAR by phosphorylation of Tyr1472-NR2B triggers phosphorylation cascade in neuropathic pain (2011)
  • Author(s): Katano T, et al
  • Organizer: 41th ANNUAL MEETING Society for Neuroscience
  • Place of Presentation: Walter E Washington Convention Genter(アメリカ合衆国)
  • Year and Date: 2011-11-16
• [Presentation] Production and function of piRNA in spermatogenesis (2011)
  • Author(s): Kuramochi-Miyagawa S
  • Organizer: アジアRNA 会議
  • Place of Presentation: 淡水福容ホテル 台湾
  • Year and Date: 2011-11-11
• [Presentation] Production and function of piRNA in spermatogenesis (2011)
  • Author(s): Satomi Kuramochi-Miyagawa
  • Organizer: アジアRNA会議
  • Place of Presentation: 淡水福容ホテル(台湾)
  • Year and Date: 2011-11-11
• [Presentation] プロテオミクス解析による神経障害性疼痛機能性分子の探索 (2011)
  • Author(s): Katano T, et al
  • Organizer: 第84回日本生化学会・大会
  • Place of Presentation: 国立京都国際会館(京都府京都市)
  • Year and Date: 2011-09-23
• [Presentation] Essential role of tyrosine phosphorylation of the NMDA receptor in mouse behavior (2011)
  • Author(s): Nakazawa T.
  • Organizer: ISN-ESN2011
  • Place of Presentation: Greece Megaron Athens International Conference Center, Greece
  • Year and Date: 2011-08-30
• [Presentation] Dopamine and tyrosine phosphorylation of NR2B (Y1472) in the hippocampus is fundamental for ERK2 activation and novel learning (2011)
  • Author(s): David O, et al
  • Organizer: ISN-ESN 2011
  • Place of Presentation: Greece Megaron Athens International Conference Center(ギリシャ)
  • Year and Date: 2011-08-30
• [Presentation] Essential role of tyrosine phosphorylation of the NMDA receptor in mouse behavior (2011)
  • Author(s): Nakazawa T
  • Organizer: ISN-ESN 2011
  • Place of Presentation: Greece Megaron Athens International Conference Center(ギリシャ)(招待講演)
  • Year and Date: 2011-08-30
• [Presentation] 精子形成におけるMVHの機能解析 (2010)
  • Author(s): 伊藤大介、宮川さとみ、仲野徹
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学年会合同学会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-09
• [Presentation] Characterization of transmembrane kinase BREK/LMTK2 in endocytic membrane trafficking (2010)
  • Author(s): Inoue T, et al
  • Organizer: 第33回日本分子生物学会・年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] 生殖幹細胞を用いたマウスPIWIファミリーの機能解析 (2010)
  • Author(s): 城本悠助、宮川さとみ、西村健、仲野徹
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] 神経因性疼痛における脊髄後角でのリン酸化シグナルカスケード (2010)
  • Author(s): 片野泰代, 他
  • Organizer: 第33回日本分子生物学会・年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-07
• [Presentation] The role of δ2-protocadherin family in synapse formation in the basal ganglia (2010)
  • Author(s): Hoshina N, et al
  • Organizer: 第33回日本分子生物学会・年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-07
• [Presentation] MVH in piRNA spermatogenesis (2010)
  • Author(s): 伊藤大介、宮川さとみ、仲野徹
  • Organizer: International Symposium on Epigenome Network. Development and Reprogramming of Germ Cells
  • Place of Presentation: 九大 医学部
  • Year and Date: 2010-11-23
• [Presentation] Analysis of MILI function by GS cells (2010)
  • Author(s): 城本悠助、宮川さとみ、西村健、仲野徹
  • Organizer: International Symposium on Epigenome Network, Development and Reprogramming of Germ Cells
  • Place of Presentation: 九大 医学部
  • Year and Date: 2010-11-22
• [Presentation] Attenuation of neuropathic pain by impaired Ca2+ signaling in mutant mice lacking the Tyr-1472 phosphorylation site of the NR2B (2010)
  • Author(s): Matsumura S, et al
  • Organizer: 40th ANNUAL MEETING Society for Neuroscience
  • Place of Presentation: San Diego/USA
  • Year and Date: 2010-11-15
• [Presentation] piRNA : its production and possible functions (2010)
  • Author(s): Kuramochi-Miyagawa S
  • Organizer: 2010 SDB and JSDB Joint Meeting
  • Place of Presentation: Albuquerque, USA
  • Year and Date: 2010-08-05
• [Presentation] piRNA : its production and possible functions (2010)
  • Author(s): Kuramochi-Miyagawa S
  • Organizer: 2010 SDB and JSDB Joint Meeting
  • Place of Presentation: Albuquerque/USA
  • Year and Date: 2010-08-05
• [Presentation] piRNA生合成におけるMVHの役割 (2010)
  • Author(s): 宮川-倉持さとみ, 他
  • Organizer: 第12回日本RNA学会年会
  • Place of Presentation: 東京 一橋記念講堂
  • Year and Date: 2010-07-27
• [Presentation] Involvement of GluN2A tyrosine phosphorylation in depression-related behavior (2010)
  • Author(s): Nakazawa T, et al
  • Organizer: 7^<th> Forum of European Neuroscience (FENS)
  • Place of Presentation: Amsterdam/the Netherlands
  • Year and Date: 2010-07-05
• [Presentation] 小脳プルキンエ細胞GluR2細胞内プールの樹状突起スパインからシャフトへの活動依存的移動の蛍光蛋白質2重発現法による解析 (2010)
  • Author(s): 山口和彦, 他
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡市民文化ホール
  • Year and Date: 2010-05-19
• [Presentation] Molecular Function of MVH on piRNA Production in Fetal Male Germ Cells (2010)
  • Author(s): Kuramochi-Miyagawa S
  • Organizer: The 19th CDB Meeting 'RNA Sciences in Cell and Developmental Biology'
  • Place of Presentation: 神戸理研(CDB)
  • Year and Date: 2010-05-12
• [Presentation] Involvement of NMDAR2A tyrosine phosphorylation in depression-related behavior (2009)
  • Author(s): 中澤敬信
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県横浜市)
  • Year and Date: 2009-12-12
• [Presentation] Involvement of NMDAR2A tyrosine phosphorylation in depression-related behavior (2009)
  • Author(s): 中澤敬信
  • Organizer: 第32回日本分子生物学会・年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Presentation] small RNAs and gene silencing in spermatogenesis (2009)
  • Author(s): 宮川さとみ
  • Organizer: 第32回日本分子生物学会・年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Presentation] The novel NYAP phosphoprotein family couples activation of Phosphoinositide 3-kinase(PI3K)p85 with the cytoskeletal WAVE signaling pathway in the brain (2009)
  • Author(s): 横山一剛
  • Organizer: 第32回日本分子生物学会・年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-11
• [Presentation] small RNA とエピジェネティック制御 (2009)
  • Author(s): 宮川さとみ
  • Organizer: 日本生化学会
  • Place of Presentation: 神戸国際会議場(兵庫県神戸市)
  • Year and Date: 2009-10-22
• [Presentation] small RNAとエピジェネティック制御 (2009)
  • Author(s): 宮川さとみ
  • Organizer: 日本生化学会(シンポジウム)
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22
• [Remarks]
  • URL: http://molpharm.umin.jp/
• [Remarks]
  • URL: http://www.fbs.osaka-u.ac.jp/labs/nakano/
• [Remarks] research map
  • URL: http://researchmap.jp/nakazawa1972/
• [Remarks] 大阪大学大学院医学系研究科病理学幹細胞病理学ラボＨＰ
  • URL: http://www.fbs.osaka-u.ac.jp/labs/nakano/
• [Remarks]
  • URL: http://researchmap.jp/nakazawa1972/