|Budget Amount *help
¥68,770,000 (Direct Cost: ¥52,900,000、Indirect Cost: ¥15,870,000)
Fiscal Year 2013: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000)
Fiscal Year 2012: ¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000)
Fiscal Year 2011: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
Fiscal Year 2010: ¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000)
Fiscal Year 2009: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
In this study, we focused on stress responses and metabolic alterations in the Drosophila apaf-1 (dpf-1) mutant, in which caspase activation and subsequent apoptosis are severely impaired. The dpf-1 mutant was found to be sensitive to injury- and starvation-stress. We show that dpf-1 mutant has defects in homeostatic gut cell renewal and that inhibiting caspase activity in fly enterocytes results in the production of systemic lethal factors after wounding. We then conducted metabolomic analyses of the hemolymph in dpf-1 mutants to investigate the physiological consequences of caspase inhibition. Several metabolites were found at higher levels in dpf-1 mutants than in wild type, which for at least one of the metabolites, was likely due to alteration of expression levels of putative metabolic enzymes in the fat body. Together, these results suggest caspase activity is required in the gut to regulate the systemic defense response in order to overcome stress in vivo.