|Budget Amount *help
¥346,320,000 (Direct Cost: ¥266,400,000、Indirect Cost: ¥79,920,000)
Fiscal Year 2013: ¥40,560,000 (Direct Cost: ¥31,200,000、Indirect Cost: ¥9,360,000)
Fiscal Year 2012: ¥40,560,000 (Direct Cost: ¥31,200,000、Indirect Cost: ¥9,360,000)
Fiscal Year 2011: ¥30,420,000 (Direct Cost: ¥23,400,000、Indirect Cost: ¥7,020,000)
Fiscal Year 2010: ¥30,420,000 (Direct Cost: ¥23,400,000、Indirect Cost: ¥7,020,000)
Fiscal Year 2009: ¥204,360,000 (Direct Cost: ¥157,200,000、Indirect Cost: ¥47,160,000)
It has been considered that the transient molecular complexes play an important role in many biological processes inside the living cells. In this project, we aimed to establish the NMR methodologies, which can observe the NMR signals from such short lived states, and reconstitute such state by mimicking physiological environments. We applied these methods to the actual biologically important systems, including chemokine-chemokine receptors, the cell adhesion molecule CD44, and the photosynthesis complex, and successfully obtained the structural information of the transiently formed complex of those molecules, which are related to their function.