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Versatile host for the heterologous expression of secondary metabolism.

Planned Research

Project AreaBiosynthetic machinery: Deciphering and regulating the system for bioactive metabolite diversification
Project/Area Number 22108006
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Science and Engineering
Research InstitutionKitasato University

Principal Investigator

IKEDA Haruo  北里大学, 感染制御科学府, 教授 (90159632)

Co-Investigator(Kenkyū-buntansha) KOMATSU Mamoru  北里大学, 大学院感染制御科学府, 講師 (40414057)
Project Period (FY) 2010-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥60,190,000 (Direct Cost: ¥46,300,000、Indirect Cost: ¥13,890,000)
Fiscal Year 2014: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
Fiscal Year 2013: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000)
Fiscal Year 2012: ¥13,780,000 (Direct Cost: ¥10,600,000、Indirect Cost: ¥3,180,000)
Fiscal Year 2011: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2010: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Keywords二次代謝産物 / 生合成マシナリー / 異種発現 / 放線菌 / テルペン化合物 / 休眠遺伝子 / 国際情報交換 / 米国 / 生合成 / 2次代謝産物 / 最適化宿主 / 物質生産 / ゲノム / 抗生物質
Outline of Final Research Achievements

An industrial microorganism Streptomyces avermitilis, which is a producer of anthelmintic macrocyclic lactones, avermectins, has been constructed as a versatile model host for heterologous expression of genes encoding secondary metabolite biosynthesis. Twenty of the entire biosynthetic gene clusters for secondary metabolites were successively cloned and introduced into a versatile model host S. avermitilis SUKA17 or 22. Almost all S. avermitilis transformants carrying the entire gene cluster produced metabolites as a result of the expression of biosynthetic gene clusters introduced. A few transformants were unable to produce metabolites but their production was restored by the expression of biosynthetic genes using an alternative promoter or the expression of a regulatory gene in the gene cluster that controls the expression of biosynthetic genes in the cluster using an alternative promoter.

Report

(6 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (64 results)

All 2015 2014 2013 2012 2011 2010 Other

All Journal Article (36 results) (of which Peer Reviewed: 34 results,  Open Access: 7 results,  Acknowledgement Compliant: 6 results) Presentation (18 results) (of which Invited: 4 results) Book (4 results) Remarks (5 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Structural basis for the 4'-hydroxylation of diclofenac by a microbial cytochrome P450 monooxygenase.2015

    • Author(s)
      L.-H. Xu, H. Ikeda, L. Liu, T. Arakawa, T. Wakagi, H. Shoun, S. Fushinobu
    • Journal Title

      Appl. Microbiol. Biotechnol,

      Volume: 99 Issue: 7 Pages: 3081-3091

    • DOI

      10.1007/s00253-014-6148-y

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Identification of an Enzyme-Catalyzed [4+2]-Cycloaddition Required for Macrocyclization of Spirotetronate-Containing Polyketides2015

    • Author(s)
      T. Hashimoto, J. Hashimoto, K. Teruya, T. Hirano, K. Shin-ya, H. Ikeda, H-.W. Liu, M. Nishiyama, T. Kuzuyama
    • Journal Title

      J. Am. Chem. Soc.

      Volume: 137 Pages: 572-575

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Terpene synthases are widely distributed in bacteria2015

    • Author(s)
      Y. Yamada, T. Kuzuyama, M. Komatsu, K. Shin-ya, S. Omura, D.E. Cane, H. Ikeda
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 112 Issue: 3 Pages: 857-862

    • DOI

      10.1073/pnas.1422108112

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Novel Terpenes Generated by Heterologous Expression of Bacterial Terpene Synthase Genes in an Engineered Streptomyces Host2015

    • Author(s)
      Yuuki Yamada, Shiho Arima, Tohru Nagamitsu, Kohei Johmoto, Hidehiro Uekusa, Tadashi Eguchi, Kazuo Shin-ya, David E. Cane, Haruo Ikeda
    • Journal Title

      J. Antibiot.

      Volume: 68 Issue: 6 Pages: 385-394

    • DOI

      10.1038/ja.2014.171

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Total synthesis of (+)-clavulatriene A2015

    • Author(s)
      F. Ito, Y. Ohbatake, S. Aoyama, T. Ikeda, S. Arima, Y. Yamada, H. Ikeda, T. Nagamitsu
    • Journal Title

      Synthesis

      Volume: 47 Issue: 09 Pages: 1348-1355

    • DOI

      10.1055/s-0034-1379898

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genome Mining Reveals a Minimum Gene Set for the Biosynthesis of 32-Membered Macrocyclic Thiopeptides Lactazoles2014

    • Author(s)
      S. Hayashi, T. Ozaki, S. Asamizu, H. Ikeda, S. Omura, N. Oku, Y. Igarashi, H. Tomoda, H. Onaka
    • Journal Title

      Chem. Biol.

      Volume: 21 Issue: 5 Pages: 679-688

    • DOI

      10.1016/j.chembiol.2014.03.008

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Discovery of gene cluster for mycosporine-like amino acid biosynthesis from Actinomycetales microorganisms and production of a novel mycosporine-like amino acid by heterologous expression2014

    • Author(s)
      K. T. Miyamoto, M. Komatsu, H. Ikeda
    • Journal Title

      Appl. Environ. Microbiol.

      Volume: 80 Issue: 16 Pages: 5028-5036

    • DOI

      10.1128/aem.00727-14

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Genome mining of the Streptomyces avermitilis genome and development of genome-minimized hosts for heterologous expression of biosynthetic gene clusters2014

    • Author(s)
      Ikeda, h. Shin-ya, K. Omura, S
    • Journal Title

      Journal of Industrial Microbiology Biotechnology

      Volume: 41 Issue: 2 Pages: 233-250

    • DOI

      10.1007/s10295-013-1327-x

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Engineered Streptomyces Avermitilis Host for Heterologous Expression of Biosynthetic Gene Cluster for Secondary Metabolites2013

    • Author(s)
      M. Komatsu, K. Komatsu, H. Koiwai, Y. Yarnada, I. Kozone, M. Izumikawa, J. Hashimoto, M. Takagi, S. Omura, K. Shin-ya, D. E. Cane, and H. Ikeda
    • Journal Title

      ACS Synth. Biol.

      Volume: 2 Issue: 7 Pages: 384-396

    • DOI

      10.1021/sb3001003

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 有用物質生産における次世代基盤技術の開発2013

    • Author(s)
      池田 治生
    • Journal Title

      バイオサイエンスとインダストリー

      Volume: 71 Pages: 492-498

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Product-Mediated Regulation of Pentalenolactone Biosynthesis in Streptomyces by the MarR/SlyA Family Activators PenR and PntR.2013

    • Author(s)
      Zhu, D.
    • Journal Title

      J. Bacteriol.

      Volume: 195 Issue: 6 Pages: 1255-1266

    • DOI

      10.1128/jb.02079-12

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Complete genome sequence of the motile actinomycete Actinoplanes missouriensis 431(T) (= NBRC 102363(T))2012

    • Author(s)
      Hideki Yamamura
    • Journal Title

      Standards in Genomic Sciences

      Volume: 2 Issue: 2 Pages: 294-303

    • DOI

      10.4056/sigs.3196539

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pleiotropic control of secondary metabolism and morphological development by KsbC, a butyrolactone autoregulator receptor homologue in Kitasatospora setae.2012

    • Author(s)
      Aiyada Aroonsri
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 78 Issue: 22 Pages: 8015-8124

    • DOI

      10.1128/aem.02355-12

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Kitasetaline, a novel β-carboline alkaloid from Kitasatospora setae NBRC 14216T2012

    • Author(s)
      Aiyada Aroonsri
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 114 Issue: 1 Pages: 56-58

    • DOI

      10.1016/j.jbiosc.2012.02.027

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular cloning of the gene cluster for lariatin biosynthesis of Rhodococcus jostii K01-B01712012

    • Author(s)
      Inokoshi J, Matsuhama M, Miyake M, Ikeda H, Tomoda H.
    • Journal Title

      Appl Microbiol Biotechnol

      Volume: (In press) Issue: 2 Pages: 451-460

    • DOI

      10.1007/s00253-012-3973-8

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The nonantibiotic small molecule cyslabdan enhances the potency of β-lactams against MRSA by inhibiting pentaglycine interpeptide bridge synthesis.2012

    • Author(s)
      Koyama N, Tokura Y, Münch D, Sahl HG, Schneider T, Shibagaki Y, Ikeda H, Tomoda H.
    • Journal Title

      PLoS One.

      Volume: 7 Issue: 11 Pages: e48981-e48981

    • DOI

      10.1371/journal.pone.0048981

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Designing of Streptomyces host for the secondary metabolite production2012

    • Author(s)
      Haruo Ikeda
    • Journal Title

      IFO, Research Communications

      Volume: 26 Pages: 159-173

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Total synthesis and absolute configuration of avenolide, extracellular factor in Streptomyces avermitilis2011

    • Author(s)
      Nagamitsu, T., Uchida, M., Takamatsu, S., Arima, S.Miyamoto, K., Kitani, S.Nihira, T., Ikeda, H.
    • Journal Title

      J Antibiotics Nature

      Volume: 64 Issue: 12 Pages: 781-787

    • DOI

      10.1038/ja.2011.90

    • NAID

      10030640894

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Avenolide, a Streptomyces hormone controlling antibiotic production in Streptomyces avermitilis2011

    • Author(s)
      Kitani, S, Miyamoto, K T, Takamatsu, S, Herawati, E, Iguchi, H, Nishimoto, K, Uchida, M, Nagamitsu, T, Omura, S, Ikeda, H, Nihira, T.
    • Journal Title

      Proc Natl Acad Sci

      Volume: 108 Issue: 39 Pages: 16410-16415

    • DOI

      10.1073/pnas.1113908108

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cloning and characterization of Pfl_1841, a 2-methylenebomane synthase in Pseudomonas fluorescens PfO-12011

    • Author(s)
      Chu, W.K.W., et al.
    • Journal Title

      Tetrahedron

      Volume: 67 Issue: 35 Pages: 6627-6632

    • DOI

      10.1016/j.tet.2011.05.084

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reveromycin A biosynthesis uses RevG and RevJ for stereospecific spiroacetal formation2011

    • Author(s)
      Takahashi S
    • Journal Title

      Nat.Chem.Biol.

      Volume: 7 Issue: 7 Pages: 461-468

    • DOI

      10.1038/nchembio.583

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The autoregulator-receptor homologue AvaR3 plays a regulatory role in antibiotic production, mycelial aggregation and colony development of Streptomyces avermitilis2011

    • Author(s)
      Miyamoto, K., Kitani, S., Komatsu, M., Ikeda, H., Nihira, T.
    • Journal Title

      Microbiology

      Volume: 157 Issue: 8 Pages: 2266-2275

    • DOI

      10.1099/mic.0.048371-0

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genome mining in Streptomyces. Elucidation of the role of Baeyer-Villiger monooxygenases and non-heme iron-dependent dehydrogenase/oxygenases in the final steps of the biosynthesis of pentalenolactone and neopentalenolactone2011

    • Author(s)
      Seo, M-J., et al.
    • Journal Title

      Biochemistry

      Volume: 50 Issue: 10 Pages: 1739-1754

    • DOI

      10.1021/bi1019786

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genome mining in Streptomyces. Discovery of an unprecedented P450-catalyzed oxidative rearrangement that is the final step in the biosynthesis of pentalenolactone2011

    • Author(s)
      Zhu, D.Seo, M-J.Ikeda, H.Cane, D.E.
    • Journal Title

      J.Am.Chem.Soc.

      Volume: 133 Issue: 7 Pages: 2128-2131

    • DOI

      10.1021/ja111279h

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of a silent sesquiterpenoid biosynthetic pathway in Streptomyces avermitiliscontrolling epi-isozizaene and albaflavenone biosynthesis and isolation of a new oxidized epi-isozizaene metabolite2011

    • Author(s)
      Takamatsu, S., et al.
    • Journal Title

      Microb.Biotechnol.

      Volume: 4 Issue: 2 Pages: 184-191

    • DOI

      10.1111/j.1751-7915.2010.00209.x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biosynthesis of 2-methylisobomeol in Cyanobacteria2011

    • Author(s)
      Giglio, S., et al.
    • Journal Title

      Environ.Sci.Technol.

      Volume: 45 Issue: 3 Pages: 992-998

    • DOI

      10.1021/es102992p

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 物質生産のための放線菌ゲノムのデザイン2011

    • Author(s)
      池田治生
    • Journal Title

      バイオサイエンスとインダストリー

      Volume: 69 Pages: 274-278

    • NAID

      10029236977

    • Related Report
      2011 Annual Research Report
  • [Journal Article] 物質生産のためのStreptomyces avermitilisゲノムの改変2011

    • Author(s)
      池田治生
    • Journal Title

      化学と生物

      Volume: 49 Pages: 738-740

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Genome mining in Streptomyces. Discovery of an unprecedented P450-catalyzed oxidative rearrangement that is the final step in the biosynthesis of pentalenolactone.2011

    • Author(s)
      Zhu, D., et al.
    • Journal Title

      J.Am.Chem.Soc.

      Volume: (in press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of a silent sesquiterpenoid biosynthetic pathway in Streptomyces avermitilis controlling epi-isozizaene and albaflavenone biosynthesis and isolation of a new oxidized epi-isozizaene metabolite.2011

    • Author(s)
      Takamatsu, S., et al.
    • Journal Title

      Microl.Biotechnol.

      Volume: 4 Pages: 184-191

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biosynthesis of 2-methylisoborneol in Cyanobacteria.2011

    • Author(s)
      Giglio, S., et al.
    • Journal Title

      Environ.Sci.Technol.

      Volume: 45 Pages: 992-998

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pentalenic acid is a shunt metabolite in the biosynthesis of the pentale nolactone family of metabolites : Hydroxylation of 1-deoxypentalenic acid mediated by CYP105D7 (SAV_7469) of Streptomyces avermitilis.2011

    • Author(s)
      Takamatsu, S., et al.
    • Journal Title

      J.Antibiot.

      Volume: 64 Pages: 65-71

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genome sequence of Kitasatospora setae NBRC 14216^T : An evolutionary snapshot of the family Streptomycetaceae.2010

    • Author(s)
      Ichikawa, N.., et al.
    • Journal Title

      DNA Res.

      Volume: 17 Pages: 393-406

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regio- and stereospecificity of filipin hydroxylation sites revealed by crystal structure of cytochrome P450 105P1 and 105D6 from Streptomyces avermitilis.2010

    • Author(s)
      Lin, X., et al.
    • Journal Title

      J.Biol.Chem.

      Volume: 285 Pages: 16844-16853

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genome mining in Streptomyces avermitilis : Cloning and characterization of SAV_76, the synthase for a new sesquiterpene. avermitilol.2010

    • Author(s)
      Chou, W.K.W., et al.
    • Journal Title

      J.Am.Chem.Soc.

      Volume: 132 Pages: 8850-8851

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genome-minimized Streptomyces host for the heterologous expression of secondary metabolism.2010

    • Author(s)
      Komatsu, M., et al.
    • Journal Title

      Proc.Natl.Acad.Sci.USA

      Volume: 107 Pages: 2646-2651

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Novel terpenes generated by heterologous expression of genes encoding bacterial terpene synthases2015

    • Author(s)
      H. Ikeda
    • Organizer
      Natural Product, Discovery & Development in the post genomic era
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2015-01-11 – 2015-01-14
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Synthetic Biology of Secondary Metabolite Production in Streptomyces2014

    • Author(s)
      Ikeda, H.
    • Organizer
      The 7th Japan-Korea Chemical Biology Symposium
    • Place of Presentation
      Jeju, Korea
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Second version of versatile hosts for heterologous expression of biosynthetic gene(s) for secondary metabolites2013

    • Author(s)
      Ikeda, H.
    • Organizer
      1st European Conference on Natural Products: Research and Applications
    • Place of Presentation
      Frankfurt, Germany
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Genome mining: an approach to finding bioactive Secondary Metabolites in Streptomyces sp. strain K1552013

    • Author(s)
      Silvia, G-T. Ikeda, H. Sanchez, S.
    • Organizer
      12th International Symposium on Genetics of Industrial Microorganisms
    • Place of Presentation
      Cancun, Mexico
    • Related Report
      2013 Annual Research Report
  • [Presentation] Engineered Streptomyces host for heterologous expression of biosyntheic gene cluster for secondary metabolites2013

    • Author(s)
      Ikeda, H. Komatsu, M. Komatsu, K. Koiwai, H. Yamada, Y. Kozone, I. Izumikawa, M. Hashimoto, J. Takagi, M. Omura, S. Shin-ya, K. Cane, DE
    • Organizer
      12th International Symposium on Genetics of Industrial Microorganisms
    • Place of Presentation
      Cancun, Mexico
    • Related Report
      2013 Annual Research Report
  • [Presentation] Heterologous expression for biosynthetic gene cluster in a versatile Streptomyces host2012

    • Author(s)
      Haruo Ikeda
    • Organizer
      International Conference of Natural Products Biosynthesis (ICNPB) 8th US-Japan Seminar on the Biosynthesis og Natural Products
    • Place of Presentation
      Awaji, Japan
    • Year and Date
      2012-06-17
    • Related Report
      2012 Annual Research Report
  • [Presentation] Discovery of new branch of pentalenolactone family tree and two different region-specific Baeyer-Villiger monooxygenases2012

    • Author(s)
      Ikeda, H, Cane, D.E.
    • Organizer
      The 6^<th> Korea-Japan Chemical Biology Symposium
    • Place of Presentation
      Sapporo, Japan(招待講演)
    • Year and Date
      2012-01-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] Avernolide, a new signaling molecule for antibiotic production in Streptomyces2012

    • Author(s)
      Miyamoto, K., et al
    • Organizer
      The 6^<th> Korea-Japan Chemical Biology Symposium
    • Place of Presentation
      Sapporo, Japan(招待講演)
    • Year and Date
      2012-01-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] 物質生産における合成生物学2012

    • Author(s)
      池田 治生
    • Organizer
      岡山県農林水産総合センター生物科学研究所 第12回RIBSシンポジウム
    • Place of Presentation
      岡山市
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Engineered Streptomyces host for heterologous expression of secondary metabolism2011

    • Author(s)
      Ikeda, H.
    • Organizer
      ESF-EMBO Symposium on "Synthetic Biology of Antibiotic Production"
    • Place of Presentation
      Sant Feliu de Guixols, Spain(招待講演)
    • Year and Date
      2011-10-06
    • Related Report
      2011 Annual Research Report
  • [Presentation] ゲノム情報からの細菌テルペン合成酵素の包括的解析2011

    • Author(s)
      池田治生
    • Organizer
      日本生物工学会大会シンポジウム
    • Place of Presentation
      東京農工大小金井キャンパス・東京(招待講演)
    • Year and Date
      2011-09-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] Synthetic Biology of the Secondary Metabolism in the Industrial Microorganism2011

    • Author(s)
      Ikeda, H.
    • Organizer
      International Union of Microbiological Societies 2011
    • Place of Presentation
      Sapporo, Japan(招待講演)
    • Year and Date
      2011-09-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] Discovery of new branch of pentalenolactone family tree and two different region-specific Baeyer-Villiger monooxygenases2011

    • Author(s)
      Ikeda, H., Cane, D.E.
    • Organizer
      International Union of Microbiological Societies 2011
    • Place of Presentation
      Sapporo, Japan(招待講演)
    • Year and Date
      2011-09-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] The genome-minimized Streptomyces hosts for the secondary metabolism2011

    • Author(s)
      Ikeda, H.
    • Organizer
      BIT'S 4th Annual World Congress of Industrial Biotechnology
    • Place of Presentation
      Dalian, China(招待講演)
    • Year and Date
      2011-04-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] Exploring the bacterial terpenome2010

    • Author(s)
      Ikeda, H.
    • Organizer
      RIKEN Chemical Biology International Symposium
    • Place of Presentation
      Wako, Japan(Invited Symposium)
    • Year and Date
      2010-10-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] Discovery of new sesquiterpenoid compounds, "Avermitilol" and "Avermitilone" by genome mining of Streptomyces avermitilis2010

    • Author(s)
      Ikeda, H.
    • Organizer
      2010 KSBB Fall Meeting & International Symposium.
    • Place of Presentation
      Incheon, Korea(Invited Symposium)
    • Year and Date
      2010-10-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] Characterization of relA mutant of industrial microorganism Streptomyces avermitilis2010

    • Author(s)
      Komatsu, M.Tanaka, Y.Ochi, K.Ikeda, H.
    • Organizer
      11^<th> International Symposium on the Genetics of Industrial Microorganisms
    • Place of Presentation
      Melbourne, Australia
    • Year and Date
      2010-06-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] Optimized Streptomyces host for the heterologous expression of secondary metabolism.2010

    • Author(s)
      Ikeda, H.Komatsu, M.Uchiyama, T.
    • Organizer
      11^<th> International Symposium on the Genetics of Industrial Microorganisms
    • Place of Presentation
      Melbourne, Australia(Invited Symposium)
    • Year and Date
      2010-06-29
    • Related Report
      2010 Annual Research Report
  • [Book] 化学と生物2014

    • Author(s)
      異種発現による天然化合物創製
    • Total Pages
      6
    • Publisher
      日本農芸化学会
    • Related Report
      2014 Annual Research Report
  • [Book] Microbial Production, From genome design to cell engineering, "Genome design of actinomycetes for secondary metabolism"2014

    • Author(s)
      Miyamoto, KT, IKeda, H.
    • Total Pages
      10
    • Publisher
      Springer
    • Related Report
      2013 Annual Research Report
  • [Book] Methods in Enzymology Vol. 515 (Natural Product Biosynthesis by Microorganisms and Plants, Part A, D.A. Hopwood ed.) Chapter seven pp.123-1662012

    • Author(s)
      Yuuki Yamada
    • Total Pages
      411
    • Publisher
      Elsevier Inc. Academic Press
    • Related Report
      2012 Annual Research Report
  • [Book] 微生物を活用した新世代の有用物質生産技術 pp.42-482012

    • Author(s)
      池田 治生
    • Total Pages
      223
    • Publisher
      シーエムシー出版
    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://avermitilis.ls.kitasato-u.ac.jp

    • Related Report
      2014 Annual Research Report
  • [Remarks] Genome Project of Streptomyces avermitilis

    • URL

      http://avermitilis.ls.kitasato-u.ac.jp

    • Related Report
      2013 Annual Research Report
  • [Remarks]

    • URL

      http://avermitilis.ls.kitasato-u.ac.jp

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://avermitilis.ls.kitasato-u.ac.jp

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://avermitilis.1s.kitasato-u.ac.jp/

    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] 1.微生物を用いたマイコスポリン様アミノ酸を生産する方法2014

    • Inventor(s)
      池田治生、曽田匡洋、松本淳、山本省吾
    • Industrial Property Rights Holder
      池田治生、曽田匡洋、松本淳、山本省吾
    • Industrial Property Rights Type
      特許
    • Filing Date
      2014-05-13
    • Related Report
      2014 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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