| Budget Amount *help |
¥60,190,000 (Direct Cost : ¥46,300,000、Indirect Cost : ¥13,890,000)
Fiscal Year 2014 : ¥12,610,000 (Direct Cost : ¥9,700,000、Indirect Cost : ¥2,910,000)
Fiscal Year 2013 : ¥13,130,000 (Direct Cost : ¥10,100,000、Indirect Cost : ¥3,030,000)
Fiscal Year 2012 : ¥13,780,000 (Direct Cost : ¥10,600,000、Indirect Cost : ¥3,180,000)
Fiscal Year 2011 : ¥13,650,000 (Direct Cost : ¥10,500,000、Indirect Cost : ¥3,150,000)
Fiscal Year 2010 : ¥7,020,000 (Direct Cost : ¥5,400,000、Indirect Cost : ¥1,620,000)
|
|---|
| Outline of Final Research Achievements |
An industrial microorganism Streptomyces avermitilis, which is a producer of anthelmintic macrocyclic lactones, avermectins, has been constructed as a versatile model host for heterologous expression of genes encoding secondary metabolite biosynthesis. Twenty of the entire biosynthetic gene clusters for secondary metabolites were successively cloned and introduced into a versatile model host S. avermitilis SUKA17 or 22. Almost all S. avermitilis transformants carrying the entire gene cluster produced metabolites as a result of the expression of biosynthetic gene clusters introduced. A few transformants were unable to produce metabolites but their production was restored by the expression of biosynthetic genes using an alternative promoter or the expression of a regulatory gene in the gene cluster that controls the expression of biosynthetic genes in the cluster using an alternative promoter.
|
