| Budget Amount *help |
¥34,970,000 (Direct Cost: ¥26,900,000、Indirect Cost: ¥8,070,000)
Fiscal Year 2014: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2013: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2012: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2011: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2010: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
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| Outline of Final Research Achievements |
In order to systematize between relationships between peptide sequences and enzyme functions including reaction mechanism, we constructed enzyme database (KNApSAcK Motorcycle DB, http://kanaya.naist.jp/motorcycle/top2.html) involving secondary metabolites in the scientific literature. The DB comprises 2,421 secondary metabolic reactions. Enzyme reactions can be retrieved using keywords of the enzymes, species, genes, metabolites and peptide sequences obtained from a BLASTP search. In the metabolite search using its keywords, we obtain information on enzyme name, reaction equation, compound class and subclass of metabolic reactions and reaction mechanisms. In the BLASTP search, we can comprehensively predict reaction equations for a targeted peptide sequence using information on the class and subclass of metabolic pathways. Thus, the Motorcycle DB makes it possible to predict enzyme reactions based on the class and subclass of metabolic reactions evidenced by experiments.
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