| Budget Amount *help |
¥150,020,000 (Direct Cost: ¥115,400,000、Indirect Cost: ¥34,620,000)
Fiscal Year 2014: ¥26,130,000 (Direct Cost: ¥20,100,000、Indirect Cost: ¥6,030,000)
Fiscal Year 2013: ¥27,560,000 (Direct Cost: ¥21,200,000、Indirect Cost: ¥6,360,000)
Fiscal Year 2012: ¥31,980,000 (Direct Cost: ¥24,600,000、Indirect Cost: ¥7,380,000)
Fiscal Year 2011: ¥31,720,000 (Direct Cost: ¥24,400,000、Indirect Cost: ¥7,320,000)
Fiscal Year 2010: ¥32,630,000 (Direct Cost: ¥25,100,000、Indirect Cost: ¥7,530,000)
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| Outline of Final Research Achievements |
Hippocampus is involved in the encoding an episodic experience into a physical trace in brain. However, how an episode is represented at cellular resolution, how long it lasts, and whether the same representation is reinstated upon recall are still not fully elucidated due to the lack of method to longitudinally observe representation. We performed two-photon microscopic Ca2+-imaging of ~1000 CA1 pyramidal neurons from mouse dorsal hippocampus during a familiar episodic event under virtual reality over two weeks. The hippocampal representation consists of a group of neurons firing sequentially during the event; ~2% of the cells fire at specific time point consistently while ~20% join the representation on each day and turn over in a few days. A change in the episode sequence largely eliminates both components, even the animal is behaviorally recalling the episode indicating the retrieval of a familiar episodic sequence can take place without a reinstatement of the same representation.
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