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Visualizing biological functions at many different scales

Planned Research

Project AreaMutli-dimensional fluorescence live imaging of cellular function and molecular activity
Project/Area Number 22113003
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

MIYAWAKI Atsushi  独立行政法人理化学研究所, 脳科学総合研究センター, チームリーダー (80251445)

Co-Investigator(Kenkyū-buntansha) HAMA Hiroshi  独立行政法人理化学研究所, 脳科学総合研究センター, 専門職研究員 (30261796)
SASAKI Kazuki  独立行政法人理化学研究所, 脳科学総合研究センター, 研究員 (10415169)
SHIMOZONO Satoshi  独立行政法人理化学研究所, 脳科学総合研究センター, 研究員 (40391982)
NIINO Yusuke  独立行政法人理化学研究所, 脳科学総合研究センター, 研究員 (10584584)
Co-Investigator(Renkei-kenkyūsha) SAKAUE Asako  独立行政法人理化学研究所, 脳科学総合研究センター, 研究員 (90462689)
KAWANO Hiroyuki  独立行政法人理化学研究所, 脳科学総合研究センター, 研究員 (10332256)
FUKANO Takashi  独立行政法人理化学研究所, 脳科学総合研究センター, 研究員 (80373364)
ANDO Ryoko  独立行政法人理化学研究所, 脳科学総合研究センター, 研究員 (10706170)
Project Period (FY) 2010-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥156,000,000 (Direct Cost: ¥120,000,000、Indirect Cost: ¥36,000,000)
Fiscal Year 2014: ¥29,120,000 (Direct Cost: ¥22,400,000、Indirect Cost: ¥6,720,000)
Fiscal Year 2013: ¥30,550,000 (Direct Cost: ¥23,500,000、Indirect Cost: ¥7,050,000)
Fiscal Year 2012: ¥31,980,000 (Direct Cost: ¥24,600,000、Indirect Cost: ¥7,380,000)
Fiscal Year 2011: ¥31,720,000 (Direct Cost: ¥24,400,000、Indirect Cost: ¥7,320,000)
Fiscal Year 2010: ¥32,630,000 (Direct Cost: ¥25,100,000、Indirect Cost: ¥7,530,000)
Keywordsイメージング / 蛍光たんぱく質 / 細胞周期 / 酸化ストレス / 透明化試薬 / レチノイン酸 / FRET / 細胞内結晶 / 蛍光蛋白質 / 酸化プローブ
Outline of Final Research Achievements

Using population and time-lapse imaging analyses of cancer cells expressing new versions of the fluorescent cell-cycle indicator, Fucci, we found great diversity in the cell-cycle alterations induced by anticancer drugs. Drug-induced cell cycle modulation varied not only between different cell types or following treatment with different drugs, but also between cells treated with different concentrations of the same drug. Using newly developed probes for retinoic acid (RA), GEPRA, we performed live imaging of zebrafish embryos at the gastrula and somitogenesis stages, which revealed a linear concentration gradient of endogenous RA in a two-tailed source-sink arrangement across the embryo. We developed a simple yet efficient method, Scale, that increases the transparency of biological tissue while preserving the signals of fluorescent labels. We established procedures for large-scale high-resolution 3D reconstruction of labeled structures within fixed specimens of millimeter size.

Report

(5 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (30 results)

All 2015 2014 2013 2012 2011 2010 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (18 results) (of which Invited: 15 results) Remarks (1 results)

  • [Journal Article] A Diffraction-Quality Protein Crystal Processed as an Autophagic Cargo2015

    • Author(s)
      Tsutsui H, Jinno Y, Shoda K,Tomita A, Matsuda M,Yamashita E, Katayama H ,Nakagawa A,Miyawaki A.
    • Journal Title

      Mol. Cell

      Volume: 58 Issue: 1 Pages: 186-193

    • DOI

      10.1016/j.molcel.2015.02.007

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance.2015

    • Author(s)
      Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, *Zeng H
    • Journal Title

      Neuron

      Volume: 85 Issue: 5 Pages: 942-958

    • DOI

      10.1016/j.neuron.2015.02.022

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Molecular spies for bioimaging - Fluorescent protein-based probes.2015

    • Author(s)
      Miyawaki A, Niino Y.
    • Journal Title

      Mol. Cell

      Volume: in press

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Visualizing spatiotemporal dynamics of multicellular cell-cycle progressions with fucci technology.2014

    • Author(s)
      Sakaue-Sawano A, Miyawaki A.
    • Journal Title

      Cold Spring Harb Protoc.

      Volume: 5 Issue: 5 Pages: 525-531

    • DOI

      10.1101/pdb.prot080408

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Bilirubin-Inducible Fluorescent Protein from Eel Muscle.2013

    • Author(s)
      Kumagai A, Ando R, Miyatake H, Greimel P, Kobayashi T, Hirabayashi Y, Shimogori T, Miyawaki A.
    • Journal Title

      Cell

      Volume: 153 Issue: 7 Pages: 1602-1611

    • DOI

      10.1016/j.cell.2013.05.038

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Contrasting Quiescent G0 Phase with Mitotic Cell Cycling in the Mouse Immune System.2013

    • Author(s)
      Tomura M, Sakaue-Sawano A, Mori Y, Takase-Utsugi M, Hata A, Ohtawa K, Kanagawa O, Miyawaki A.
    • Journal Title

      Plos One

      Volume: 8 Issue: 9 Pages: e73801-e73801

    • DOI

      10.1371/journal.pone.0073801

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Visualizing developmentally programmed endoreplication in mammals using ubiquitin oscillators2013

    • Author(s)
      Sakaue-Sawano A, Hoshida T, Yo M, Takahashi R, Ohtawa K, Arai T, Takahashi E, Noda S, Miyoshi H, Miyawaki A.
    • Journal Title

      Development

      Volume: 140 Issue: 22 Pages: 4624-4632

    • DOI

      10.1242/dev.099226

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Visualization of an endogenous retinoic acid gradient across embryonic development2013

    • Author(s)
      Shimozono S. Iimura T. Kitaguchi T. Higashijima S. Miyawaki A
    • Journal Title

      Nature

      Volume: 496(7445) Issue: 7445 Pages: 363-6

    • DOI

      10.1038/nature12037

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Visualization of cell cycle in mouse embryos with Fucci2 reporter directed by Rosa 26 promoter.2013

    • Author(s)
      Abe T
    • Journal Title

      Development

      Volume: 140 Issue: 1 Pages: 237-246

    • DOI

      10.1242/dev.084111

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Red fluorescent proteins: chromophore formation and cellular applications.2012

    • Author(s)
      Miyawaki A, Shcherbakova DM, Verkhusha VV.
    • Journal Title

      Curr. Opin. Struct. Biol.

      Volume: 22 Issue: 5 Pages: 679-688

    • DOI

      10.1016/j.sbi.2012.09.002

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication2011

    • Author(s)
      Sakaue-Sawano, et al.
    • Journal Title

      BMC Cell Biology

      Volume: 12:2 Pages: 1-12

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Cruising inside cells2015

    • Author(s)
      Atsushi Miyawaki
    • Organizer
      「細胞機能と分子活性の多次元蛍光生体イメージング」国際シンポジウム
    • Place of Presentation
      京都
    • Year and Date
      2015-01-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] Cruising inside cells2014

    • Author(s)
      宮脇敦史
    • Organizer
      日本動物遺伝育種学会第15回年次大会
    • Place of Presentation
      埼玉
    • Year and Date
      2014-11-01
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Cruising inside cells2014

    • Author(s)
      宮脇敦史
    • Organizer
      第35回日本肥満学会「肥満症学の知の創造と実践」
    • Place of Presentation
      宮崎
    • Year and Date
      2014-10-25
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] A Bilirubin-Inducible Fluorescent Protein from Eel Muscle2014

    • Author(s)
      Atsushi Miyawaki, Akiko Kumagai, Ryoko Ando
    • Organizer
      Janelia Conference on Fluorescent Proteins and Biological Sensors IV
    • Place of Presentation
      Janelia Farm, USA
    • Year and Date
      2014-09-28 – 2014-10-01
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Cruising inside cells2014

    • Author(s)
      宮脇敦史
    • Organizer
      第57回日本腎臓学会学術総会
    • Place of Presentation
      東京
    • Year and Date
      2014-07-05
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Cruising inside Xs2014

    • Author(s)
      宮脇敦史
    • Organizer
      日本実験動物科学・技術さっぽろ
    • Place of Presentation
      札幌
    • Year and Date
      2014-05-16
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] The interplay between light and life2013

    • Author(s)
      宮脇敦史
    • Organizer
      第1回最先端創薬科学シンポジウム 『蛍光と創薬』
    • Place of Presentation
      長崎大学
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 新規蛍光プローブとバイオサイエンスの新展開2013

    • Author(s)
      宮脇敦史
    • Organizer
      JASIS 2013英国王立化学会(RSC)東京国際コンファレンス
    • Place of Presentation
      幕張
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Cruising inside X2013

    • Author(s)
      宮脇敦史
    • Organizer
      26th International Conference on Photochemistry
    • Place of Presentation
      Leuven, Belgium
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Cruising inside cells2013

    • Author(s)
      宮脇敦史
    • Organizer
      第60回日本実験動物学会総会
    • Place of Presentation
      つくば
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Cruising Inside X2013

    • Author(s)
      Atsushi Miyawaki
    • Organizer
      The 10th NIBB-EMBL Symposium 2013 on Quantitative Bioimaging
    • Place of Presentation
      Okazaki, Japan
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Visualizing spatiotemporal dynamics of multicellular cell-cycle progression2013

    • Author(s)
      Atsushi Miyawaki
    • Organizer
      AACR-SNMMI Joint Conference on State-of-the-Art Molecular Imaging in Cancer Biology and Therapy
    • Place of Presentation
      San Diego, USA
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] New fluorescent probes and new perspectives in bioscience2012

    • Author(s)
      宮脇敦史
    • Organizer
      第35回日本分子生物学会年会13
    • Place of Presentation
      福岡
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 蛍光タンパク質の最前線2012

    • Author(s)
      宮脇敦史
    • Organizer
      生物発光化学発光研究会 第29回 学術講演会
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] New fluorescent probes and new perspectives in bioscience2012

    • Author(s)
      Atsushi Miyawaki
    • Organizer
      Neuroinformatics 2012
    • Place of Presentation
      Munich, Germany
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] New fluorescent probes and new perspectives in bioscience2012

    • Author(s)
      Atsushi Miyawaki
    • Organizer
      The 14th International Congress on Histochemistry and Cytochemistry
    • Place of Presentation
      Kyoto、Japan
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] ScaLE: A Chemical Approach For High-Resolution Fluorescence Imaging And 3d Reconstruction Of Transparent Mouse Brain2012

    • Author(s)
      Hiroshi Hama
    • Organizer
      The FENS Forum of Neuroscience 2012
    • Place of Presentation
      Barcelona, Spain
    • Related Report
      2012 Annual Research Report
  • [Presentation] 3D quantifying the association of proliferative neural stem cell nuclei with blood vessels in the SGZ2010

    • Author(s)
      宮脇敦史
    • Organizer
      Janelia Farm conference "Light-based approaches to neural circuit reconstruction"
    • Place of Presentation
      Janelia Farm, USA
    • Year and Date
      2010-10-25
    • Related Report
      2010 Annual Research Report
  • [Remarks] 研究室紹介

    • URL

      http://www.brain.riken.jp/jp/faculty/details/30

    • Related Report
      2012 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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