|Budget Amount *help
¥50,050,000 (Direct Cost: ¥38,500,000、Indirect Cost: ¥11,550,000)
Fiscal Year 2014: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Fiscal Year 2013: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Fiscal Year 2012: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2011: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2010: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
|Outline of Final Research Achievements
Dysfunction in intracellular signal transduction is a cause of many diseases. Majority of computational signal transduction research has been focused on pathways. Since a cell is 3D entity, we tested the effect of spatial parameters on the signal transduction.
First we found that oscillation of transcription factor NF-kB was regulated by diffusion coefficient, nuclear transport, and nuclear/cytoplasmic volume ratio, and in addition, while diffusion coefficient and export for mRNA from the nucleus regulated persistency of NF-kB oscillation, IkB import to the nucleus exclusively regulated oscillation frequency. Second, we found that the fusion of stress granules (SGs) was important for their dynamics, and simulation predicted gamma distribution of SG size, which was confirmed by experiments.