|Budget Amount *help
¥94,640,000 (Direct Cost: ¥72,800,000、Indirect Cost: ¥21,840,000)
Fiscal Year 2014: ¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2013: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2012: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2011: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2010: ¥24,960,000 (Direct Cost: ¥19,200,000、Indirect Cost: ¥5,760,000)
|Outline of Final Research Achievements
Cystathionine γ-synthase (CGS) catalyzes the first committed step of methionine biosynthesis in plants. Expression of CGS1 gene that encodes CGS is feedback-regulated, in response to S-adenosyl-L-methonine (SAM), by translation arrest at Ser-94 codon followed by mRNA degradation. Studies using in vitro translation system revealed that nascent peptide of CGS1 takes compact conformation upon translation arrest, and conformation of 28S rRNA and/or interaction between the nascent peptide and rRNA changes. Upon translation arrest, ribosomes are stacked behind the initially arrested ribosome at Ser-94. We revealed that the stacked ribosomes are arranged at nine codon intervals.
During general mRNA degradation, poly(A) shortening is the first and usually the rate-limiting step. In SAM-induced CGS1 mRNA degradation, however, the poly(A) shortening was not observed.