|Budget Amount *help
¥97,240,000 (Direct Cost : ¥74,800,000、Indirect Cost : ¥22,440,000)
Fiscal Year 2014 : ¥18,070,000 (Direct Cost : ¥13,900,000、Indirect Cost : ¥4,170,000)
Fiscal Year 2013 : ¥18,980,000 (Direct Cost : ¥14,600,000、Indirect Cost : ¥4,380,000)
Fiscal Year 2012 : ¥20,020,000 (Direct Cost : ¥15,400,000、Indirect Cost : ¥4,620,000)
Fiscal Year 2011 : ¥19,760,000 (Direct Cost : ¥15,200,000、Indirect Cost : ¥4,560,000)
Fiscal Year 2010 : ¥20,410,000 (Direct Cost : ¥15,700,000、Indirect Cost : ¥4,710,000)
|Outline of Final Research Achievements
Protein complexes are essential for various kinds of physiological functions in the human body. By developing single particle analysis (SPA) using Transmission electron microscopy (TEM), the three-dimensional (3D) structure of signal processing protein complexes. Further, these structures including SecDF are interpreted by docking partial structure determined by X-ray crystallography.
The new atmospheric scanning electron microscope (ASEM) observes samples through electron-transparent film. Therefore, samples can be set in solution under an open atmosphere. Using this system, immuno-ASEM was successfully developed, and we have observed intracellular super-molecular-complex formations, including accumulation of STIM-1 in response to Ca2+ store depletion. Moreover, the observation method of small protein crystals are developed, and contributed to X-ray crystallography. Further, by multi-windowning ASEM, the observation efficiency was improved.