| Budget Amount *help |
¥98,670,000 (Direct Cost: ¥75,900,000、Indirect Cost: ¥22,770,000)
Fiscal Year 2014: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2013: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2012: ¥20,410,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥4,710,000)
Fiscal Year 2011: ¥20,150,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥4,650,000)
Fiscal Year 2010: ¥21,060,000 (Direct Cost: ¥16,200,000、Indirect Cost: ¥4,860,000)
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| Outline of Final Research Achievements |
We have investigated molecular mechanisms underlying insect leg regeneration by means of regeneration-dependent RNA interference. Our results suggested that leg regeneration occurs as follows:
(1) Activation of macrophage-like cells by amputation at the amputated site, (2) Cytokines produced by the macrophage-like cells induce formation of a blastema consisting of undifferentiated cells, (3) In the blastema, EGF (epidermal growth factor) is induced by cross-talk of Wnt/BMP signaling pathways. Furthermore, genes involved in epigenetic regulations such as Enhancer of Zest and Utx are activated, (4) EGF induces expressions of dachshund/Distal-less and regulates expression of Dachsous/Fat signaling systems, which then determine re-patterning of the leg and its size. We speculated that the leg size regulation may be related to the number of Dachsous/Fat molecules in each cell membrane with a proximodistal gradient. This was simulated with our modified steepness model.
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