Role of stress-induced epigenetic change in genome adaptation

• Project Area: Systematic study of chromosome adaptation
• Project/Area Number: 22125005
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: ISHII Shunsuke 独立行政法人理化学研究所, 石井分子遺伝学研究室, 上席研究員 (00124785)
• Project Period (FY): 2010-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥129,870,000 (Direct Cost: ¥99,900,000、Indirect Cost: ¥29,970,000); Fiscal Year 2014: ¥24,180,000 (Direct Cost: ¥18,600,000、Indirect Cost: ¥5,580,000); Fiscal Year 2013: ¥25,480,000 (Direct Cost: ¥19,600,000、Indirect Cost: ¥5,880,000); Fiscal Year 2012: ¥26,650,000 (Direct Cost: ¥20,500,000、Indirect Cost: ¥6,150,000); Fiscal Year 2011: ¥26,390,000 (Direct Cost: ¥20,300,000、Indirect Cost: ¥6,090,000); Fiscal Year 2010: ¥27,170,000 (Direct Cost: ¥20,900,000、Indirect Cost: ¥6,270,000)
• Keywords: エピゲノム / 遺伝 / ストレス / 環境要因 / エオイジェネティクス / テロメア / 寿命 / ATF7 / 疾患 / エピジェネティクス / ATF-2 / エピジェネティック変化 / ヘテロクロマチン / ヒストン / H3-K9メチル化 / ゲノム / 適応 / ヒストン修飾 / ATF-7 / エピジェネテクス

Outline of Final Research Achievements

It was shown that transcription factor ATF-2 mediates the heat shock stress-induced epigenetic change which is transmitted to next generation. The ATF-2 family transcription factors are also involved in the innate immune memory and the psychological stress-induced telmere shortening. Furthermore, they mediates the epigenome change induced by nutritional condition and psychological stress which are transmitted to next generation. Thus, ATF-2 family transcription afctors contribute to the epigenome change induced by various environmental factors and its inheritance.

Research Products

• [Journal Article] Disruption of Th2a and Th2b genes causes defects in spermatogenesis. (2015)
  • Author(s): Shinagawa, H. et al.
  • Journal Title: Development Volume: 142 Pages: 1287-1292
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] T396I mutation of mouse Sufu reduces the stability and activity of Gli3 repressor. (2015)
  • Author(s): Makino, S. et al.
  • Journal Title: PLoS One Volume: 10
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Histone variants enriched in oocytes enhance reprogramming to induced pluripotent stem cells (2014)
  • Author(s): Shinagawa T, Takagi T, Tsukamoto D, Tomaru C, Huynh LM, Sivaraman P, Kumarevel T, Inoue K, Nakato R, Katou Y, Sado T, Takahashi S, Ogura A, Shirahige K, Ishii S
  • Journal Title: Cell Stem Cell Volume: 14(2) Issue: 2 Pages: 217-227
  • DOI: 10.1016/j.stem.2013.12.015
  • Peer Reviewed
• [Journal Article] Ubiquitination-deubiquitination by the TRIM27-USP7 complex regulates tumor necrosis factor alpha-induced apoptosis. (2013)
  • Author(s): 1. Zaman MM, Nomura T, Takagi T, Okamura T, Jin W, Shinagawa T, Tanaka Y, Ishii S
  • Journal Title: Mol Cell Biol Volume: 33 Issue: 24 Pages: 4971-4984
  • DOI: 10.1128/mcb.00465-13
  • Peer Reviewed
• [Journal Article] Trim27-deficient mice are susceptible to streptozotocin-induced diabetes. (2013)
  • Author(s): Zaman MM, Ishii S 他
  • Journal Title: FEBS Open Bio. Volume: 4 Issue: 1 Pages: 60-64
  • DOI: 10.1016/j.fob.2013.12.002
  • Peer Reviewed
• [Journal Article] Su(fu) switches Rdx functions to fine-tune hedgehog signaling in the Drosophila wing disk (2013)
  • Author(s): Seong KH & Ishii S
  • Journal Title: Genes Cells Volume: 18 Issue: 1 Pages: 66-78
  • DOI: 10.1111/gtc.12018
  • Peer Reviewed
• [Journal Article] Deficiency of schnurri-2, an MHC enhancer binding protein, induces mild chronic inflammation in the brain and confers molecular, neuronal, and behavioral phenotypes related to schizophrenia. (2013)
  • Author(s): Takao K, Kobayashi K, Hagihara H, Ohira K, Shoji H, Hattori S, Koshimizu H, Umemori J, Toyama K, Nakamura HK, Kuroiwa M, Maeda J, Atsuzawa K, Esaki K, Yamaguchi S, Furuya S, Takagi T, Walton NM, Hayashi N, Suzuki H, Higuchi M, Usuda N, Suhara T, Nishi A, Matsumoto M, Ishii S, Miyakawa T.
  • Journal Title: Neuropsychopharmacology Volume: 38(8) Issue: 8 Pages: 1409-25
  • DOI: 10.1038/npp.2013.38
  • Peer Reviewed / Open Access
• [Journal Article] Inheritance and memory of stress-induced epigenome change : roles played by the ATF-2 family of transcription factors (2012)
  • Author(s): Seong KH, Maekawa T & Ishii S
  • Journal Title: Genes Cells Volume: 17 Issue: 4 Pages: 249-263
  • DOI: 10.1111/j.1365-2443.2012.01587.x
  • Peer Reviewed
• [Journal Article] Fbxw5 suppresses nuclear c-Myb activity via DDB1-Cul4-Rbx1 ligase-mediated sumoylation (2012)
  • Author(s): Kanei-Ishii C, Nomura T, Egoh A & Ishii S
  • Journal Title: Biochem Biophys Res Commun Volume: 426 Issue: 1 Pages: 59-64
  • DOI: 10.1016/j.bbrc.2012.08.032
  • Peer Reviewed
• [Journal Article] Inheritance and memory of stress-induced epigenome change : roles played by the ATF-2 family of transcription factors (2012)
  • Author(s): Seong KH, Maekawa T, Ishii S
  • Journal Title: Genes to Cells Volume: 17(In press)
  • Peer Reviewed
• [Journal Article] Inheritance of stress-induced, ATF-2-dependent epigenetic change (2011)
  • Author(s): Seong KH, Li D, Shimizu H, Nakamura R, Ishii S
  • Journal Title: Cell Volume: 145 Issue: 7 Pages: 1049-1061
  • DOI: 10.1016/j.cell.2011.05.029
  • Peer Reviewed
• [Journal Article] Dampening of death pathways by schnurri-2 is essential for T-cell development (2011)
  • Author(s): Staton TL, Lazarevic V, Jones DC, Lanser AJ, Takagi T, Ishii S, Glimcher LH
  • Journal Title: Nature Volume: 472 Issue: 7341 Pages: 105-109
  • DOI: 10.1038/nature09848
  • Peer Reviewed
• [Journal Article] Schnurri-2 deficiency counteracts against bone loss induced by ovariectomy. (2011)
  • Author(s): Nagao M., et al.
  • Journal Title: J Cell Physiol Volume: 226 Pages: 573-578
  • Peer Reviewed
• [Journal Article] Conditional knockdown of target gene expression by tetracycline regulated transcription of double strand RNA. (2011)
  • Author(s): Hou, X., et al.
  • Journal Title: Dev Growth Differ. Volume: 53 Pages: 69-75
  • Peer Reviewed
• [Journal Article] Dampening of death pathways by Schnurri-2 is essential for T cell development. (2011)
  • Author(s): Staton, T.L., et al.
  • Journal Title: Nature Volume: (In press)
  • Peer Reviewed
• [Journal Article] Social isolation stress induces ATF-7 phosphorylation and impairs silencing of the 5-HT 5B receptor gene. (2010)
  • Author(s): Maekawa, T., et al.
  • Journal Title: EMBO J. Volume: 29 Pages: 196-208
  • Peer Reviewed
• [Journal Article] The role of ATF-2 family transcription factors in adipocyte differentiation : anti-obesity effects of p38 inhibitors. (2010)
  • Author(s): Maekawa, T., et al.
  • Journal Title: Mol.Cell.Biol. Volume: 30 Pages: 613-625
  • Peer Reviewed
• [Journal Article] Uncoupling of growth plate maturation and bone formation in mice lacking both Schnurri-2 and Schnurri-3. (2010)
  • Author(s): Jones, D.C., et al.
  • Journal Title: Proc.Natl.Acad.Sci.USA. Volume: 107 Pages: 8254-8258
  • Peer Reviewed
• [Journal Article] Inhibition of the nuclear import of cubitus interruptus by roadkill in the presence of strong hedgehog signal. (2010)
  • Author(s): Seong, K.H., et al.
  • Journal Title: PLOS One Volume: 5
  • Peer Reviewed
• [Presentation] 転写因子ATF7を介したストレスによるテロメアの長さの制御 (2014)
  • Author(s): 前川利男、Liu Binbin、吉田圭介、仲村賢一、田久保海誉、石井俊輔
  • Organizer: 第３７回に本分子生物学会年会
  • Place of Presentation: 横浜、パシフィコ横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] 父親への拘束ストレスによるエピゲノム変化と遺伝の解析 (2014)
  • Author(s): 成耆鉉、石井俊輔
  • Organizer: 第３７回に本分子生物学会年会
  • Place of Presentation: 横浜、パシフィコ横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] 精子形成におけるヒストンバリアントTH2AとTH2Bの役割 (2014)
  • Author(s): 品川敏恵、Huynh Linh My、高木豪、塚本大輔、都丸千夏、野口純子、石井俊輔
  • Organizer: 第３７回に本分子生物学会年会
  • Place of Presentation: 横浜、パシフィコ横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Inheritance of stress-induced chromatin alterations. (2013)
  • Author(s): Ishii S
  • Organizer: Gordon Conference on Epigenetics
  • Place of Presentation: Bryant University, Smithfield, RI, USA
  • Invited
• [Presentation] ATF-7 controls epigenetic memory of pathogen infection in macrophages. (2013)
  • Author(s): Yoshida K, Ishii S 他
  • Organizer: Gordon Conference on Epigenetics
  • Place of Presentation: Bryant University, Smithfield, RI, USA
• [Presentation] Inheritance of stress-induced epigenome changes. (2013)
  • Author(s): Ishii S
  • Organizer: Small RNAs to Stem Cells & Epigenetic Reprograming Asia-2013 Meeting
  • Place of Presentation: Univ. of Tokyo, Tokyo, Japan
  • Invited
• [Presentation] 拘束ストレスにより誘導されるエピゲノム変化の遺伝 (2013)
  • Author(s): 成耆鉉、石井俊輔
  • Organizer: 第３６回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場、神戸
• [Presentation] 転写因子ATF-7を介したストレスによるテロメアの長さの制御 (2013)
  • Author(s): 前川利男、石井俊輔 他
  • Organizer: 第３６回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場、神戸
• [Presentation] 卵子に大量に存在するヒストンバリアントによる 核のリプログラミング (2013)
  • Author(s): 品川敏恵、石井俊輔 他
  • Organizer: 第３６回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場、神戸
• [Presentation] Ubiquitination-deubiquitination by the TRIM27-USP7 complex regulates TNF-α-induced apoptosis (2013)
  • Author(s): Zaman MM, Ishii S 他
  • Organizer: 第３６回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場、神戸
• [Presentation] Role of ATF-2 family transcription factors in inheritance of epigenetic change induced by stress (2012)
  • Author(s): 石井俊輔
  • Organizer: 第45回日本発生生物学会・第64回日本細胞生物学会合同大会
  • Place of Presentation: 神戸
  • Invited
• [Presentation] ショウジョウバエにおけるストレス依存的エピジェネティック制御とその遺伝の解析 (2012)
  • Author(s): 成耆鉉
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] マクロファージの炎症時におけるATF-7の機能解析 (2012)
  • Author(s): 吉田圭介
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] 転写因子ATF-7を介したストレスによるテロメアの長さの制御 (2012)
  • Author(s): 前川利男
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] Deficiency of Schnurri-2, an MHC enhancer binding protein, induces mild chronic inflammation in the brain and confers molecular, neuronal, and behavioral phenotypes related to schizophrenia (2012)
  • Author(s): 宮川剛
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] ストレスによるエピゲノム変化の遺伝 (2012)
  • Author(s): 石井俊輔
  • Organizer: 第85回日本生化学会年会
  • Place of Presentation: 福岡
  • Invited
• [Presentation] ストレスはATF-7を介してヘテロクロマチン形成とテロメアの長さを制御する (2011)
  • Author(s): 前川利男, 吉田圭介, Binbin Liu, 仲村賢一, 田久保海誉, 石井俊輔
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-14
• [Presentation] ATF-7は炎症の抑制因子として機能する (2011)
  • Author(s): 吉田圭介, 前川利男, Claire Renard-Guillet, 白髭克彦, 石井俊輔
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-14
• [Presentation] Inheritance of stress-induced epigenome change :research using fly and mouse (2011)
  • Author(s): 石井俊輔
  • Organizer: 第34回日本分子生物学会年会ワークショップ
  • Place of Presentation: 横浜(招待講演)
  • Year and Date: 2011-12-13
• [Presentation] 環境・代謝ストレスによるエピジェネティック変化と疾患 (2011)
  • Author(s): 石井俊輔
  • Organizer: 第2回Molecular Cardiovascular Conference II
  • Place of Presentation: 小樽(招待講演)
  • Year and Date: 2011-09-02
• [Presentation] ATF-2ファミリー転写因子とエピジェネティック制御 (2010)
  • Author(s): 石井俊輔
  • Organizer: 阪大蛋白研セミナー
  • Place of Presentation: 大阪
  • Year and Date: 2010-11-20
• [Presentation] Inheritance of stress-induced epigenetic change (2010)
  • Author(s): 石井俊輔
  • Organizer: 第15回東大・分生研シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2010-11-02
• [Remarks]
  • URL: http://www.riken.go.jp/r-world/info/release/press/2011/110624/detail.html
• [Patent(Industrial Property Rights)] Methods for generating nuclear reprogrammed cell, and use thereof. (2012)
  • Inventor(s): 品川敏恵、石井俊輔
  • Industrial Property Rights Holder: 品川敏恵、石井俊輔
  • Industrial Property Rights Type: 特許
  • Filing Date: 2012-08-29
  • Overseas