|Budget Amount *help
¥148,070,000 (Direct Cost: ¥113,900,000、Indirect Cost: ¥34,170,000)
Fiscal Year 2014: ¥27,170,000 (Direct Cost: ¥20,900,000、Indirect Cost: ¥6,270,000)
Fiscal Year 2013: ¥28,600,000 (Direct Cost: ¥22,000,000、Indirect Cost: ¥6,600,000)
Fiscal Year 2012: ¥40,040,000 (Direct Cost: ¥30,800,000、Indirect Cost: ¥9,240,000)
Fiscal Year 2011: ¥42,120,000 (Direct Cost: ¥32,400,000、Indirect Cost: ¥9,720,000)
Fiscal Year 2010: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
|Outline of Final Research Achievements
Asymmetric cell division is a fundamental mechanism to produce cellular diversity. In C. elegans, asymmetric divisions are regulated by Wnt signaling, resulting in asymmetric nuclear localization of β-catenin after the divisions. We showed that this localization is regulated by spindle that is formed asymmetrically through cortical APC protein. The results show the presence of new logic that regulates protein nuclear localization by modulating microtubules in cytoplasm.
During development of C. elegans, most cells are polarized in the same orientation with asymmetric β-catenin localization after their divisions. We showed that, in epithelial stem cells, four Wnt genes redundantly controls such coordinated polarity. Polarity orientations are abnormal in the absence of Wnts. But Wnts can properly function even when they are ectopically expressed. Therefore, coordinated cell polarity is controlled through unknown logic.