| Research Collaborator |
Daimon Takaaki 東京大学, 大学院農学生命科学研究科, 助教
Fujii Tsuguru 日本学術振興会, 特別研究員PD
Kiuchi Takashi 東京大学, 大学院農学生命科学研究科, 助教
Wang Huabing 日本学術振興会, 外国人特別研究員
Katsuma Susumu 東京大学, 大学院農学生命科学研究科, 准教授
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| Budget Amount *help |
¥109,980,000 (Direct Cost: ¥84,600,000、Indirect Cost: ¥25,380,000)
Fiscal Year 2014: ¥21,320,000 (Direct Cost: ¥16,400,000、Indirect Cost: ¥4,920,000)
Fiscal Year 2013: ¥20,280,000 (Direct Cost: ¥15,600,000、Indirect Cost: ¥4,680,000)
Fiscal Year 2012: ¥23,400,000 (Direct Cost: ¥18,000,000、Indirect Cost: ¥5,400,000)
Fiscal Year 2011: ¥21,060,000 (Direct Cost: ¥16,200,000、Indirect Cost: ¥4,860,000)
Fiscal Year 2010: ¥23,920,000 (Direct Cost: ¥18,400,000、Indirect Cost: ¥5,520,000)
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| Outline of Final Research Achievements |
The larval food habits of bombycoid silkmoths have been presumably evolved from Ficus feeding to Morus (mulberry) one. The latex of mulberry contains sugar-mimic alkaloidal toxins, and the mulberry feeders (Bombyx etc.) must overcome the toxicity. The polyphagous mutants, Bt and spli were analyzed to understand the genetic basis of mulberry feeding in Bombyx. We found that a transcription factor gene (Bmacj6) was defective in the mutants. The expression of the genes encoding olfactory receptors and so on was over- or mis-expressed in sensory organs of the mutants. On the other hand, we found that the major sucrases were enzymatically more tolerant to the mulberry alkaloids in Bombyx than in non-mulberry bombycoids. The results of RNA-seq, genome sequencing, and genome editing suggested that the sucrases and other enzymes influenced by the sugar-mimic alkaloids have evolved quantitatively and qualitatively so that the mulberry-feeding species have overcome the toxicity.
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