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Transcription-coupled repair and protein remodeling

Planned Research

Project AreaCoupling of replication, repair and transcription, and their common mechanism of chromatin remodeling
Project/Area Number 22131009
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionOsaka University

Principal Investigator

TANAKA Kiyoji  大阪大学, 生命機能研究科, 特任教授 (80144450)

Project Period (FY) 2010-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥129,870,000 (Direct Cost: ¥99,900,000、Indirect Cost: ¥29,970,000)
Fiscal Year 2014: ¥24,180,000 (Direct Cost: ¥18,600,000、Indirect Cost: ¥5,580,000)
Fiscal Year 2013: ¥25,480,000 (Direct Cost: ¥19,600,000、Indirect Cost: ¥5,880,000)
Fiscal Year 2012: ¥26,650,000 (Direct Cost: ¥20,500,000、Indirect Cost: ¥6,150,000)
Fiscal Year 2011: ¥26,390,000 (Direct Cost: ¥20,300,000、Indirect Cost: ¥6,090,000)
Fiscal Year 2010: ¥27,170,000 (Direct Cost: ¥20,900,000、Indirect Cost: ¥6,270,000)
Keywordsヌクレオチド除去修復 / 転写と共役した修復 / 色素性乾皮症 / コケイン症候群 / 紫外線高感受性症候群 / ユビキチン化 / SUMO化 / 初期応答遺伝子 / 転写 / FOS遺伝子発現 / RNAポリメラーゼII / UVSSA / USP7 / XPG / FOS遺伝子 / c-fos / XPF-ERCC1 / CSB / ヌクレオソーム / ユビキチンリガーゼ / MMXD複合体 / TFIIH
Outline of Final Research Achievements

We examined molecular mechanism of transcription-coupled nucleotide excision repair (TC-NER) that quickly removes transcription-blocking DNA damage on the transcribed strands, and leads to resumption of transcription after DNA damage. We found that (1) Ubiquitylation of the largest subunit of RNA polymerase II by the CSA complex is indispensable for TC-NER. (2) Novel TC-NER factor, UVSSA-USP7 complex, is required for protection of CSB from proteasome-mediated degradation after UV-irradiation, leading to resumption of transcription. (3) We revealed novel functions of NER factors, XPG and XPD. XPG is required for transcription elongation of primary response gene FOS and house keeping gene EEF1A1, and XPD is included in the novel protein complex, named as MMXD, which is involved in chromosome segregation.

Report

(6 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (46 results)

All 2015 2014 2013 2012 2011 2010 Other

All Journal Article (16 results) (of which Peer Reviewed: 15 results,  Acknowledgement Compliant: 2 results,  Open Access: 1 results) Presentation (26 results) (of which Invited: 6 results) Book (1 results) Remarks (3 results)

  • [Journal Article] Constructive rescue of TFIIH instability by an alternative isoform of XPD derived from a mutated XPD allele in mild but not severe XP-D/CS.2015

    • Author(s)
      Katsuyoshi Horibata, Sayaka Kono, Chie Ishigami, Xue Zhang, Madoka Aizawa, Yuko Kako, Takuma Ishii, Rika Kosaki, Masafumi Saijo and Kiyoji Tanaka
    • Journal Title

      J. Human Genetics

      Volume: 印刷中 Issue: 5 Pages: 259-265

    • DOI

      10.1038/jhg.2015.18

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Our research over the past 40 years on the mechanisms underlying and disorders associated with nucleotide excision repair2014

    • Author(s)
      Kiyoji Tanaka
    • Journal Title

      Photomedicine and Photobiology

      Volume: 36 Pages: 11-18

    • Related Report
      2014 Annual Research Report
  • [Journal Article] Nucleotide excision repair-dependent DNA double-strand break formation and ATM signaling activation in mammalian quiescent cells2014

    • Author(s)
      Mitsuo Wakasugi, Takuma Sasaki, Megumi Matsumoto, Miyuki Nagaoka, Keiko Inoue, Manabu Inobe, Katsuyoshi Horibata, Kiyoji Tanaka, and Tsukasa Matsunaga
    • Journal Title

      J. Biological Chemistry

      Volume: 289 Issue: 41 Pages: 28730-28737

    • DOI

      10.1074/jbc.m114.589747

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] IOP1 protein is an external component of the human cytosolic iron-sulfur cluster assembly (CIA) machinery and functions in the MMS19 protein-dependent CIA pathway2013

    • Author(s)
      Mineaki Seki, Yukiko Takeda, Kazuhiro Iwai and Kiyoji Tanaka
    • Journal Title

      Journal of Biological Chemistry

      Volume: 288 Issue: 23 Pages: 16680-16689

    • DOI

      10.1074/jbc.m112.416602

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Mutations in UVSSA cause UV-sensitive syndrome and destabilize ERCC6 in transcription-coupled DNA repair2012

    • Author(s)
      X. Zhang
    • Journal Title

      Nat.Genet

      Volume: 44 Issue: 5 Pages: 593-597

    • DOI

      10.1038/ng.2228

    • Related Report
      2012 Annual Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The conserved Cockayne syndrome B-piggyBac fusion protein (CSB-PGBD3) affects DNA repair and induces both interferon-like and innate antiviral responses in CSB-null cells2012

    • Author(s)
      Arnold D.Bailey
    • Journal Title

      DNA Repair

      Volume: (In press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Xeroderma pigmentosum group F protein binds to Eg5 and is required for proper mitosis : Implications for XP-F and XFE2012

    • Author(s)
      Li Jing Tan
    • Journal Title

      Genes to Cells

      Volume: 17 Issue: 3 Pages: 173-185

    • DOI

      10.1111/j.1365-2443.2012.01582.x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] BRCA1 contributes to transcription-coupled repair of DNA damage through polyubiquitination and degradation of Cockayne syndrome B protein.2011

    • Author(s)
      Wei L, Lan L, Yasui A, Tanaka K, Saijo M, Matsuzawa A, Kashiwagi R, Maseki E, Hu Y, Parvin J D, Ishioka C and Chiba N
    • Journal Title

      Cancer Science

      Volume: 102(10) Issue: 10 Pages: 1840-7

    • DOI

      10.1111/j.1349-7006.2011.02037.x

    • NAID

      10030243954

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nucleotide excision repairby mutant xeroderma pigmentosum groupA (XPA) proteins with deficiency in interaction with RPA.2011

    • Author(s)
      Masafumi Saijo
    • Journal Title

      Journal of Biological Chemistry

      Volume: 286 Pages: 5476-5483

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mutant Cockayne syndrome group B protein inhibits repair of DNA topoisomerase I-DNA covalent complex.2011

    • Author(s)
      Katsuyoshi Horibata
    • Journal Title

      Genes to Cells

      Volume: 16 Pages: 101-114

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Japanese trichothiodystrophy patient with XPD mutations.2011

    • Author(s)
      Touhei Usuda
    • Journal Title

      Journal of Human Genetics

      Volume: 56 Pages: 77-79

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spermatogenesisis not impaired in a nucleotide excision repair-deficient Min mouse model with or without neonatal mutagen.2011

    • Author(s)
      Ashild, Andreassen
    • Journal Title

      International Journal of Andrology

      Volume: (In press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] MMXD, a TFIIH-independent XPD-MMS19 protein complex involved in chromosome segregation.2010

    • Author(s)
      Shinsuke Ito
    • Journal Title

      Molecular Cell

      Volume: 39 Pages: 632-640

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The DDB2 complex-mediated ubiquitylation around DNA damage is oppositely regulated by XPC and Ku, and contributes to the recruitment of XPA.2010

    • Author(s)
      Arato Takedachi
    • Journal Title

      Molecular and Cellular Biology

      Volume: 30 Pages: 2708-2723

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Influences of p53 deficiency on the apoptotic response, DNA damage removal and mutagenesis in UVB-exposed mouse skin.2010

    • Author(s)
      Hironobu Ikehata
    • Journal Title

      Mutagenesis

      Volume: 25 Pages: 397-405

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] No inhibitory effects of (-)-epigallocatechin gallate and lycopene on spontaneous hepatocarcinogenesis in C3H/HeN mice.2010

    • Author(s)
      Yoshihisa Takahashi
    • Journal Title

      Fukushima J.Medical Science

      Volume: 56 Pages: 17-27

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] XPD-TFIIH複合体による転写伸長反応の制御機構の解析2014

    • Author(s)
      成田 央、成田 圭子、竹立 新人、田中 亀代次
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Regulation of transcription elongation by XPG-TFIIH complex is implicated in Cockayne syndrome2014

    • Author(s)
      Kiyoji Tanaka
    • Organizer
      5th US-Japan DNA Repair Meeting
    • Place of Presentation
      Grand XIV Naruto(Naruto city, Tokushima prefecture)
    • Year and Date
      2014-10-28 – 2014-10-31
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] ヌクレオチド除去修復研究の40年:過去から未来へ2014

    • Author(s)
      田中 亀代次
    • Organizer
      第36回光医学・光生物学会
    • Place of Presentation
      大阪大学銀杏会館(吹田市)
    • Year and Date
      2014-07-25 – 2014-07-26
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] XPG, a nucleotide excision repair factor is required for transactivation and transcription elongation: Implication for the pathogenesis of XP-G/CS2014

    • Author(s)
      Kiyoji Tanaka
    • Organizer
      International Symposium on “XP & Related Diseases:Disorders of DNA Damage Response - Bench to Bedside
    • Place of Presentation
      神戸国際会議場(神戸市)
    • Year and Date
      2014-03-05 – 2014-03-07
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Functional analysis of CSB in transcription-coupled repair2014

    • Author(s)
      Yooksil Sin, Masafumi Saijo, Kiyoji Tanaka
    • Organizer
      International symposium on“XP & Related Diseases - DNA Damage Response Disorders - Bench to Bedside”
    • Place of Presentation
      神戸国際会議場(神戸市)
    • Year and Date
      2014-03-05 – 2014-03-07
    • Related Report
      2013 Annual Research Report
  • [Presentation] 鉄代謝の破綻により引き起こされる紫外線DNA損傷の修復、及び、複製異常2013

    • Author(s)
      関 峰秋、田中 亀代次
    • Organizer
      日本放射線影響学会第56回大会
    • Place of Presentation
      ホテルクラウンパレス青森(青森市)
    • Year and Date
      2013-10-18 – 2013-10-20
    • Related Report
      2013 Annual Research Report
  • [Presentation] Non-canonical function of nucleotide excision repair factor: Implication For XP/CS2013

    • Author(s)
      Kiyoji Tanaka
    • Organizer
      Gordon Research Conference” Mammalian DNA Repair”
    • Place of Presentation
      Ventura, CA, USA
    • Year and Date
      2013-02-10
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 転写と共役したヌクレオチド除去修復機構におけるCSBの機能解析2012

    • Author(s)
      申 育實
    • Organizer
      第35回日本分子生物学会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-11
    • Related Report
      2012 Annual Research Report
  • [Presentation] MMS19 is a cytosolic Fe-S assembly factor required for genomic integrity2012

    • Author(s)
      Mineaki Seki
    • Organizer
      8th 3R Symposium
    • Place of Presentation
      Awajishima, Hyogo, Japan
    • Year and Date
      2012-11-25
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Mechanism and disorders of transcription-coupled repair2012

    • Author(s)
      Kiyoji Tanaka
    • Organizer
      3rd Erling Seeberg Symposium
    • Place of Presentation
      Trondheim, Norway
    • Year and Date
      2012-06-19
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Molecular mechanism and inherited disorders of nucleotide excision repair2011

    • Author(s)
      田中亀代次
    • Organizer
      第34回日本分子生物学会
    • Place of Presentation
      横浜(招待講演)
    • Year and Date
      2011-12-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] Molecular cloning of the gene for UV-sensitive syndrome with Deficiency in transcription-coupled DNA repair2011

    • Author(s)
      Xue Zhang
    • Organizer
      第34回日本分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2011-12-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] UVSSA and USP7 cooperate to stabilize CSB in transcription-coupled DNA repair2011

    • Author(s)
      西條将文
    • Organizer
      第34回日本分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2011-12-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] Histological analysis of impaired spermatogenesis in xeroderma Pigmentosum group A (Xpa)-deficient mice2011

    • Author(s)
      中根裕信
    • Organizer
      第34回日本分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2011-12-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] The roles of human MMS19 in cross-link repair and FeS assembly2011

    • Author(s)
      関峰秋
    • Organizer
      第40回日本環境変異原学会
    • Place of Presentation
      東京
    • Year and Date
      2011-11-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] Molecular Mechanism and Human Inherited Diseases of Nucleotide Excision Repair2011

    • Author(s)
      Kiyoji Tanaka
    • Organizer
      Conference "Response to DNA Damage : from Molecular Mechanism to Human Disease"
    • Place of Presentation
      Egmond aan Zee, The Netherlands(招待講演)
    • Year and Date
      2011-04-03
    • Related Report
      2011 Annual Research Report
  • [Presentation] MMXD:染色体分配に関与するTFIIH非依存性XPDタンパク質複合体2010

    • Author(s)
      田中亀代次
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸(招待講演)
    • Year and Date
      2010-12-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] DDA1によるDDB2ユビキチンリガーゼ複合体形成の制御2010

    • Author(s)
      末友寿実
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] The DDB2 complex-mediated ubiquitylation around DNA damage is oppositely regulated by XPC and Ku, and contributes to the recruitment of XPA2010

    • Author(s)
      Arato Takedachi
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] Molecular cloning of the UVSSA gene for UV-sensitive syndrome with deficiency in transcription-coupled repair2010

    • Author(s)
      Xue Zhang
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] Analysis of the interaction between XPF and bipolar motor prote in Eg52010

    • Author(s)
      Li Jing Tan
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] Molecular characterization of apatient with UV-sensitivity and de novo unbalanced translocation between chromosome 6p and 10q2010

    • Author(s)
      Dawn Koh
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] DNA修復異常症におけるDNA損傷応答の違いによる脳神経病変への関与-ATLD、NBS、XPAの神経病変2010

    • Author(s)
      中根裕信
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸(招待講演)
    • Year and Date
      2010-12-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] Regulation of the formation of DDB2-E3 complex by DDA12010

    • Author(s)
      Hisami Suetomo
    • Organizer
      The 7th International 3R Symposium
    • Place of Presentation
      富山
    • Year and Date
      2010-10-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] The DDB2 complex-mediated ubiquitylation around DNA damage is oppositely regulated by XPC and Ku, and contributes to the recruitment of XPA2010

    • Author(s)
      Arato Takedachi
    • Organizer
      The 7th International 3R Symposium
    • Place of Presentation
      富山
    • Year and Date
      2010-10-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] XPD forms a TFIIH-independent protein complex involved in chromosome segregation2010

    • Author(s)
      Kiyoji Tanaka
    • Organizer
      Workshop on XERODERMA PIGMENTOSUM AND OTHER DISEASES OF HUMAN PREMATURE AGING AND DNA REPAIR : MOLECULES TO PATIENTS
    • Place of Presentation
      Virginia, USA(招待講演)
    • Year and Date
      2010-09-21
    • Related Report
      2010 Annual Research Report
  • [Book] プログレッシブ 生命科学2014

    • Author(s)
      田中 亀代次、西條将文
    • Total Pages
      312
    • Publisher
      南山堂
    • Related Report
      2014 Annual Research Report
  • [Remarks] 大阪大学大学院生命機能研究科 ヒト細胞生物学グループ

    • URL

      http://www.fbs.osaka-u.ac.jp/labo/03a.html

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.fbs.osaka-u.ac.jp/labo/03a.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.fbs.osaka-u.ac.jp/labo/03a.html

    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2018-03-28  

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