Structural analysis of HLA class I/II molecules
Project Area | HLA polymorphism, disease and evolution |
Project/Area Number |
22133004
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Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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Allocation Type | Single-year Grants |
Review Section |
Biological Sciences
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Research Institution | The Institute of Physical and Chemical Research |
Principal Investigator |
YOKOYAMA Shigeyuki 独立行政法人理化学研究所, 横山構造生物学研究室, 上席研究員 (00159229)
|
Co-Investigator(Kenkyū-buntansha) |
SHIROUZU Mikako 独立行政法人理化学研究所, ライフサイエンス技術基盤研究センター, 副センター長 (70280732)
HONMA Teruki 独立行政法人理化学研究所, ライフサイエンス技術基盤研究センター, チームリーダー (10466039)
|
Project Period (FY) |
2010-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥161,070,000 (Direct Cost: ¥123,900,000、Indirect Cost: ¥37,170,000)
Fiscal Year 2014: ¥39,780,000 (Direct Cost: ¥30,600,000、Indirect Cost: ¥9,180,000)
Fiscal Year 2013: ¥41,080,000 (Direct Cost: ¥31,600,000、Indirect Cost: ¥9,480,000)
Fiscal Year 2012: ¥26,650,000 (Direct Cost: ¥20,500,000、Indirect Cost: ¥6,150,000)
Fiscal Year 2011: ¥26,390,000 (Direct Cost: ¥20,300,000、Indirect Cost: ¥6,090,000)
Fiscal Year 2010: ¥27,170,000 (Direct Cost: ¥20,900,000、Indirect Cost: ¥6,270,000)
|
Keywords | HLA / ペプチド / 立体構造 / タンパク質 / 免疫学 / 医薬品設計 / X線結晶構造解析 / 構造生物学 / 蛋白質 / 分子認識 |
Outline of Final Research Achievements |
In this project, we performed the protein expression, purification, and crystallization of HLA molecules, HLA-DP5, HLA-DR53, and HLA-DR4, bound to their antigenic peptide. Thereby, we successfully determined the crystal structure of HLA-DP5 in complex with Cryptomeria japonica 1 peptide (pCry j 1), derived from Japanese cedar pollen. Based on this structural information, we designed ten compounds to inhibit pCry j 1 binding to HLA-DP5, as evaluation test candidates for the peptide-binding affinity. Furthermore, we determined the crystal structure of HLA-DP5 in complex with pCry j 1, including the flanking region.
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Report
(2 results)
Research Products
(3 results)
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[Journal Article] Structural basis for the specific recognition of the major antigenic peptide from the Japanese cedar pollen allergen Cry j 1 by HLA-DP52014
Author(s)
Kusano, S., Kukimoto-Niino, M., Satta, Y., Ohsawa, N., Uchikubo-Kamo, T., Wakiyama, M., Ikeda, M., Terada, T., Yamamoto, K., Nishimura, Y., Shirouzu, M., Sasazuki, T., Yokoyama, S.
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Journal Title
Journal of Molecular Biology
Volume: 426
Pages: 3016-3027
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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