Evaluation of biological activitis of natural ligands by the use of omics technology

• Project Area: Chemical Biology using bioactive natural products as specific ligands: identification of molecular targets and regulation of bioactivity
• Project/Area Number: 23102004
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Osaka University
• Principal Investigator: Watanabe Hajime 大阪大学, 工学(系)研究科(研究院), 教授 (80212322)
• Project Period (FY): 2011-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥43,290,000 (Direct Cost: ¥33,300,000、Indirect Cost: ¥9,990,000); Fiscal Year 2015: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000); Fiscal Year 2014: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000); Fiscal Year 2013: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000); Fiscal Year 2012: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000); Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
• Keywords: オミクス / 天然物リガンド / トランスジェニック動物 / 生理活性物質 / マウス / マイクロアレイ / エストロゲン / 遺伝子発現 / 生理活性 / DNAマイクロアレイ / ゲノミクス / 生理活性化合物 / 甲殻類

Outline of Final Research Achievements

Cultured cells, mice and daphnia were exposed to natural ligands and expressed RNAs were analyzed. Based on the transcriptome profiles, biological activities of the natural ligands were evaluated. To facilitate the evaluation, transgenic animals that can visualize these ligand activities were also established.

Research Products

• [Int'l Joint Research] Institute of Hydrobiology(チェコ)
• [Int'l Joint Research] Birmingham University(United Kingdom)
• [Int'l Joint Research] KU Leuven - University of Leuven(Belgium)
• [Journal Article] Betaproteobacteria Limnohabitans strains increase fecundity in the crustacean Daphnia magna: symbiotic relationship between major bacterioplankton and zooplankton in freshwater ecosystem. (2016)
  • Author(s): Peerakietkhajorn S, Kato Y, Kasalicky V, Matsuura T, Watanabe H
  • Journal Title: Environ Microbiol Volume: in press
  • DOI: 10.1111/1462-2920.12919
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Growth evaluation method by live imaging of Daphnia magna and its application to the estimation of an insect growth regulator (2015)
  • Author(s): Suzuki A, Kato Y, Matsuura T, Watanabe H
  • Journal Title: J Appl Toxicol Volume: 35 Pages: 68-74
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Symbiotic bacteria contribute to increasing the population size of a freshwater crustacean, Daphnia magna. (2015)
  • Author(s): Peerakietkhajorn S, Tsukada K, Kato Y, Matsuura T, Watanabe H
  • Journal Title: Environ Microbiol Rep Volume: 7 Pages: 364-372
  • DOI: 10.1111/1758-2229.12260
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] TALEN-mediated homologous recombination in Daphnia magna. (2015)
  • Author(s): Nakanishi T, Kato Y, Matsuura T, Watanabe H
  • Journal Title: Scientific Reports Volume: 5 Pages: 18312-18312
  • DOI: 10.1038/srep18312
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Heterodimeric TALENs induce targeted heritable mutations in the crustacean Daphnia magna (2015)
  • Author(s): 内藤彰子、加藤泰彦、中西貴士、松浦友亮、渡邉肇
  • Journal Title: Biol Open Volume: 13 Pages: 364-369
  • DOI: 10.1242/bio.20149738
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Visualization of ecdysteroid activity using a reporter gene in the crustacean, Daphnia. (2014)
  • Author(s): Asada, M., Kato, Y., Matsuura, T. and Watanabe, H.
  • Journal Title: Marine Environmental Research Volume: 93 Pages: 118-122
  • DOI: 10.1016/j.marenvres.2013.11.005
  • Peer Reviewed
• [Journal Article] Early Embryonic Expression of a Putative Ecdysteroid-Phosphate Phosphatase in the Water Flea, Daphnia magna (Cladocera: Daphniidae). (2014)
  • Author(s): Asada, M., Kato, Y., Matsuura, T. and Watanabe, H.
  • Journal Title: Journal of Insect Science Volume: 14
  • Peer Reviewed
• [Journal Article] CRISPR/Cas-Mediated Targeted Mutagenesis in Daphnia magna. (2014)
  • Author(s): Nakanishi, T., Kato, Y., Matsuura, T. and Watanabe, H.
  • Journal Title: Plos One Volume: 9
  • Peer Reviewed / Open Access
• [Journal Article] In Vitro Membrane Protein Synthesis Inside Cell-Sized Vesicles Reveals the Dependence of Membrane Protein Integration on Vesicle Volume. (2014)
  • Author(s): Soga, H., Fujii, S., Yomo, T., Kato, Y., Watanabe, H. and Matsuura, T.
  • Journal Title: ACS Synth Biol Volume: 0 Pages: 16796-16801
  • DOI: 10.1021/sb400094c
  • Peer Reviewed / Open Access
• [Journal Article] Optimization of mRNA design for protein expression in the crustacean Daphnia magna. (2014)
  • Author(s): Torner, K., Nakanishi, T., Matsuura, T., Kato, Y. and Watanabe, H.
  • Journal Title: Molecular Genetics and Genomics Volume: 289 Pages: 707-715
  • DOI: 10.1007/s00438-014-0830-8
  • Peer Reviewed
• [Journal Article] Molecular impact of juvenile hormone agonists on neonatal Daphnia magna. (2014)
  • Author(s): Toyota, K., Kato, Y., Miyakawa, H., Yatsu, R., Mizutani, T., Ogino, Y., Miyagawa, S., Watanabe, H., Nishide, H., Uchiyama, I., Tatarazako, N. and Iguchi, T.
  • Journal Title: Jounal of Applied Toxicology Volume: 34 Pages: 537-544
  • DOI: 10.1002/jat.2922
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Molecular cloning of doublesex genes of four cladocera (water flea) species (2013)
  • Author(s): Kenji Toyota, Yasuhiko Kato, Masaru Sato, Naomi Sugiura, Shinichi Miyagawa, Hitoshi Miyakawa, Hajime Watanabe, Shigeto Oda, Yukiko Ogino, Chizue Hiruta, Takeshi Mizutani, Norihisa Tatarazako, Susanne Paland, Craig Jackson, John K. Colbourne, Taisen Iguchi
  • Journal Title: BMC genomics Volume: 14:239 Pages: 239-239
  • DOI: 10.1186/1471-2164-14-239
  • Peer Reviewed
• [Journal Article] Vesnarinone Suppresses TNF α mRNA Expression by Inhibiting Valosin-containing Protein (2013)
  • Author(s): Hotta K, Nashimoto A, Yasumura E, Suzuki M, Azuma M, Iizumi Y, Shima D, Nabeshima R, Hiramoto M, Okada A, Sakata-Sogawa K, Tokunaga M, Ito T, Ando H, Sakamoto S, Kabe Y, Aizawa S, Imai T, Yamaguchi Y, Watanabe H, Handa H
  • Journal Title: Mol. Pharmacol. Volume: Vol.83 Pages: 11-15
  • DOI: 10.1124/mol.112.081935
  • Peer Reviewed
• [Journal Article] Vesnarinone Suppresses TNFα mRNA Expression by Inhibiting Valosin-containing Protein (2013)
  • Author(s): Hotta, K
  • Journal Title: Molecuｌar Pharmacology Volume: 83 Pages: 930-930
  • Peer Reviewed
• [Journal Article] Molecular cloning of doublesex genes of four cladocera (water flea) species (2013)
  • Author(s): Toyota, K
  • Journal Title: BMC Genomics Volume: 14 Pages: 239-239
  • Peer Reviewed
• [Journal Article] Genomic integration and germline transmission of plasmid injected into crustacean Daphnia magna eggs. (2012)
  • Author(s): Kato, Y
  • Journal Title: Plos Genet Volume: 7 Pages: 1001345-1001345
  • Peer Reviewed
• [Journal Article] Development of an RNA interference method in the cladoceran crustacean Daphnia magna (2011)
  • Author(s): Kato, Y.
  • Journal Title: Dev Genes Evol Volume: 220 Pages: 337-345
  • Peer Reviewed
• [Journal Article] Branchiopod Crustacean Daphinia magna : Deep Conservation of a Doublesex Gene in the Sex-Determining Pathway (2011)
  • Author(s): Kato, Y.
  • Journal Title: Plos Genet Volume: 7
  • Peer Reviewed
• [Presentation] Development of transgenic Daphnia (2015)
  • Author(s): Yasuhiko KATO, Takashi NAKANISHI, Tomoaki MATSUURA, Hajime WATANABE
  • Organizer: Pacifichem 2015
  • Place of Presentation: Hawaii, USA
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] An lncRNA regulates an environmental sex determination in Daphnia magna (2015)
  • Author(s): Yasuhiko Kato, Hajime Watanabe
  • Organizer: 第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
  • Place of Presentation: 神戸国際会議場（兵庫県･神戸市）
  • Year and Date: 2015-12-01
  • Invited
• [Presentation] Efficient DNA integration into germ line genome via TALEN in Daphnia magna (2015)
  • Author(s): Takashi Nakanishi, Yasuhiko Kato, Tomoaki Matsuura, Hajime Watanabe
  • Organizer: Conference on Transposition and Genome Engineering 2015
  • Place of Presentation: 奈良春日野国際フォーラム(奈良県･奈良市）Nara, Japan
  • Year and Date: 2015-11-17
  • Int'l Joint Research
• [Presentation] A long noncoding RNA regulates a sex-determining gene doublesex1 in the crustacean Daphnia magna (2015)
  • Author(s): Yasuhiko Kato, Hajime Watanabe
  • Organizer: EMBL Symposia "Noncoding Genome"
  • Place of Presentation: Heidelberg, Germany
  • Year and Date: 2015-10-18
  • Int'l Joint Research
• [Presentation] Efficient DNA integration into germ line genome via programmable nucleases in Daphnia magna (2015)
  • Author(s): Takashi Nakanishi, Yasuhiko Kato, Tomoaki Matsuura, Hajime Watanabe
  • Organizer: Cold Spring Harbor Laboratory Meetings & Courses Program "GENOME ENGINEERING: THE CRISPR/CAS REVOLUTION"
  • Place of Presentation: Cold Spring Harbor, USA
  • Year and Date: 2015-09-24
  • Int'l Joint Research
• [Presentation] Development of transgenic Daphnia for monitoring hormonal activities of chemicals (2015)
  • Author(s): Takashi NAKANISHI, Miki ASADA, Yasuhiko KATO, Hajime WATANABE
  • Organizer: SETAC Europe 25th Annual Meeting
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2015-05-03
  • Int'l Joint Research
• [Presentation] 細胞記憶のメカニズムを理解するためのモデルシステムとしての新生仔期DES処理マウスの膣上皮細胞 (2014)
  • Author(s): 和田忠士、.北村彩佳、.石川達也、山上修平、.真門剛毅、渡邉肇
  • Organizer: 第37回日本分子生物学会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] CRISPRﾉCaｓ9,TALENを利用したオオミジンコのゲノム編集技術の開発 (2014)
  • Author(s): 中西 貴士・内藤 彰子・加藤 泰彦・松浦 友亮・渡邉 肇
  • Organizer: 第37回日本分子生物学会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] ヘテロニ量体型TALENを用いたノックアウトミジンコの作出 (2014)
  • Author(s): 内藤 彰子・中西 貴士・加藤 泰彦・松浦 友亮・渡邉 肇
  • Organizer: 第37回日本分子生物学会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] 酸化ストレス可視化型Daphniaの創出 (2014)
  • Author(s): 渡邉 肇
  • Organizer: 環境科学会2014
  • Place of Presentation: 茨城県つくば市
  • Year and Date: 2014-09-18
• [Presentation] CRISPR/CasおよびTALENによるDaphnia magnaのゲノム編集技術の開発 (2014)
  • Author(s): 中西 貴士・内藤 彰子・加藤 泰彦・松浦 友亮・渡邉 肇
  • Organizer: 日本生物工学会
  • Place of Presentation: 北海道札幌市
  • Year and Date: 2014-09-09 – 2014-09-11
• [Presentation] Evaluation of biological activities of chemical compounds using small organisms (2014)
  • Author(s): 渡邉肇
  • Organizer: 新学術班会議
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2014-05-28 – 2014-05-29
• [Presentation] Establishment of gene knock out technology using TALEN in D.magna. (2014)
  • Author(s): 内藤彰子、中西貴士、加藤泰彦、松浦友亮、渡邉肇
  • Organizer: Daphnia Genomics Consortium MEETING 2014
  • Place of Presentation: University of Birmingham(イギリス）
• [Presentation] The long noncoding RNA DAPALR regulates expression of the sex determining gene doublesex1 in Daphnia magna (2014)
  • Author(s): 加藤泰彦
  • Organizer: Daphnia Genomics Consortium MEETING 2014
  • Place of Presentation: University of Birmingham(イギリス）
• [Presentation] Development of CRISPR/Cas9-mediated genome editing techinique in Daphnia magna (2014)
  • Author(s): 中西貴士
  • Organizer: Daphnia Genomics Consortium MEETING 2014
  • Place of Presentation: University of Birmingham(イギリス）
• [Presentation] ライブイメージングによるミジンコ成長速度定量化方法の確立 (2013)
  • Author(s): 鈴木安貴子, 加藤泰彦, 松浦友亮, 渡邉肇
  • Organizer: 第40回日本毒性学会学術年会
  • Place of Presentation: 幕張メッセ国際会議場
• [Presentation] Comparative Ecotoxicogenomics Using Medaka, Daphnia and Algae (2013)
  • Author(s): 渡邉肇
  • Organizer: ICT Seoul 2013
  • Place of Presentation: The art Coex conference center
• [Presentation] 環境によるオオミジンコの性決定機構 (2013)
  • Author(s): 加藤泰彦, 渡邉肇
  • Organizer: 生態適応グローバルCOE 生態適応セミナー
  • Place of Presentation: 東北大学
• [Presentation] 枝角目甲殻類ミジンコの環境性決定を司る幼若ホルモンの作用機構の解析 (2013)
  • Author(s): 加藤泰彦, 渡邉肇
  • Organizer: 日本進化学会第15回筑波大会
  • Place of Presentation: 筑波大学
• [Presentation] Live Imaging of Daphnia magna for Growth Evaluation. (2013)
  • Author(s): 鈴木安貴子,渡邉肇
  • Organizer: 第六回日中韓大学院生フォーラム
  • Place of Presentation: 忠南大学(韓国）
• [Presentation] Comparative Ecotoxicogenomics Using Medaka, Daphnia and Algae (2013)
  • Author(s): 渡邉肇
  • Organizer: International Environmental Omics Synthesis Conference
  • Place of Presentation: カーディフ（イギリス）
• [Presentation] オオミジンコにおけるエクジステロイト脱リン酸化酵素の機能解析 (2013)
  • Author(s): 浅田実希, 渡邉肇,加藤泰彦
  • Organizer: 日本動物学会第84回岡山大会
  • Place of Presentation: 岡山大学
• [Presentation] Long noncoding RNA transcribed from sex determining gene doulesex1 promoter in the crustacean Daphnia magna. (2013)
  • Author(s): 加藤泰彦, 須藤優海,渡邉肇
  • Organizer: EMBL Symposia "Noncoding genome"
  • Place of Presentation: European Molecular Biology Laboratory(ドイツ）
• [Presentation] リポソーム内部合成膜タンパク質はベシクルサイズ依存的に膜挿入される (2013)
  • Author(s): 曽我遥, 加藤泰彦, 渡邉肇,松浦友亮
  • Organizer: 第8回無細胞生命科学研究会
  • Place of Presentation: 新潟大学
• [Presentation] リポソーム内無細胞膜タンパク質合成系ヘのトランスロコンの導入 (2013)
  • Author(s): 太田直樹, 渡邉肇,松浦友亮
  • Organizer: 第8回無細胞生命科学研究会
  • Place of Presentation: 新潟大学
• [Presentation] CRISPR/Cas9システムを用いたDaphnia magnaのゲノム編集技術の開発 (2013)
  • Author(s): 中西貴士, 渡邉肇
  • Organizer: 第3回ゲノム編集研究会
  • Place of Presentation: 広島大学
• [Presentation] オオミジンコの性決定遺伝子 doublesex1 のプロモーター領域で発現する長鎖ノンコーディング RNAの機能解析 (2013)
  • Author(s): 加藤泰彦, 須藤優海,渡邉肇
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場他
• [Presentation] 環境指標生物Daphnia magnaでのCRISPR/Cas9システムを用いたゲノム編集技術の開発 (2013)
  • Author(s): 中西貴士, 加藤泰彦, 渡邉肇
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場他
• [Presentation] 環境指標生物Daphnia magnaにおけるMinosトランスポゾンを用いたゲノム改変技術の開発 (2012)
  • Author(s): 渡邉 肇
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡国際会議場・マリンメッセ
  • Year and Date: 2012-12-11
• [Presentation] オオミジンコにおける胚発生初期エクジステロイドの機能解析 (2012)
  • Author(s): 渡邉 肇
  • Organizer: 第37回日本比較内分泌学会
  • Place of Presentation: 福井大学
  • Year and Date: 2012-11-29
• [Presentation] オオミジンコ Daphnia magna における新規レポーターアッセイ系の確立 (2012)
  • Author(s): 渡邉 肇
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪大学
  • Year and Date: 2012-09-14
• [Presentation] ミジンコにおける環境依存的な性決定機構 (2012)
  • Author(s): 渡邉 肇
  • Organizer: 日本進化学会第14回東京大会
  • Place of Presentation: 首都大学
  • Year and Date: 2012-08-21
• [Presentation] 酸化ストレス可視化型 Daphnia magnaの創出 (2012)
  • Author(s): 渡邉 肇
  • Organizer: 環境科学会
  • Place of Presentation: 横浜国立大学
  • Invited
• [Presentation] 環境によるミジンコの性決定機構 (2011)
  • Author(s): 加藤泰彦, 渡邉肇
  • Organizer: 第13回日本進化学会大会
  • Place of Presentation: 京都(招待講演)
• [Presentation] Development of an RNA interference method in the cladoceran crustacean Daphnia magna (2011)
  • Author(s): Kato, Y., Shiga, Y., Tokishita, S-I., Watanabe, H.
  • Organizer: 8th International Congress of Comparative Physiology and Biochemistry
  • Place of Presentation: Nagoya(招待講演)
• [Remarks] 大阪大学大学院工学研究科生命先端工学専攻渡邉研究室
  • URL: http://www.bio.eng.osaka-u.ac.jp/ez/project/index.html
• [Remarks]