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Evaluation of biological activitis of natural ligands by the use of omics technology

Planned Research

Project AreaChemical Biology using bioactive natural products as specific ligands: identification of molecular targets and regulation of bioactivity
Project/Area Number 23102004
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Science and Engineering
Research InstitutionOsaka University

Principal Investigator

Watanabe Hajime  大阪大学, 工学(系)研究科(研究院), 教授 (80212322)

Project Period (FY) 2011-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥43,290,000 (Direct Cost: ¥33,300,000、Indirect Cost: ¥9,990,000)
Fiscal Year 2015: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2014: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2013: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2012: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Keywordsオミクス / 天然物リガンド / トランスジェニック動物 / 生理活性物質 / マウス / マイクロアレイ / エストロゲン / 遺伝子発現 / 生理活性 / DNAマイクロアレイ / ゲノミクス / 生理活性化合物 / 甲殻類
Outline of Final Research Achievements

Cultured cells, mice and daphnia were exposed to natural ligands and expressed RNAs were analyzed. Based on the transcriptome profiles, biological activities of the natural ligands were evaluated. To facilitate the evaluation, transgenic animals that can visualize these ligand activities were also established.

Report

(6 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (58 results)

All 2016 2015 2014 2013 2012 2011 Other

All Int'l Joint Research (3 results) Journal Article (18 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 18 results,  Acknowledgement Compliant: 4 results,  Open Access: 6 results) Presentation (35 results) (of which Int'l Joint Research: 5 results,  Invited: 2 results) Remarks (2 results)

  • [Int'l Joint Research] Institute of Hydrobiology(チェコ)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] Birmingham University(United Kingdom)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] KU Leuven - University of Leuven(Belgium)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Betaproteobacteria Limnohabitans strains increase fecundity in the crustacean Daphnia magna: symbiotic relationship between major bacterioplankton and zooplankton in freshwater ecosystem.2016

    • Author(s)
      Peerakietkhajorn S, Kato Y, Kasalicky V, Matsuura T, Watanabe H
    • Journal Title

      Environ Microbiol.

      Volume: in press Issue: 8 Pages: 2366-2374

    • DOI

      10.1111/1462-2920.12919

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Growth evaluation method by live imaging of Daphnia magna and its application to the estimation of an insect growth regulator2015

    • Author(s)
      Suzuki A, Kato Y, Matsuura T, Watanabe H
    • Journal Title

      J Appl Toxicol

      Volume: 35 Pages: 68-74

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Symbiotic bacteria contribute to increasing the population size of a freshwater crustacean, Daphnia magna.2015

    • Author(s)
      Peerakietkhajorn S, Tsukada K, Kato Y, Matsuura T, Watanabe H
    • Journal Title

      Environ Microbiol Rep

      Volume: 7 Issue: 2 Pages: 364-372

    • DOI

      10.1111/1758-2229.12260

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] TALEN-mediated homologous recombination in Daphnia magna.2015

    • Author(s)
      Nakanishi T, Kato Y, Matsuura T, Watanabe H
    • Journal Title

      Sci Rep

      Volume: 5 Issue: 1 Pages: 18312-18312

    • DOI

      10.1038/srep18312

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Heterodimeric TALENs induce targeted heritable mutations in the crustacean Daphnia magna2015

    • Author(s)
      内藤彰子、加藤泰彦、中西貴士、松浦友亮、渡邉肇
    • Journal Title

      Biology OPEN

      Volume: 13 Issue: 3 Pages: 364-369

    • DOI

      10.1242/bio.20149738

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Visualization of ecdysteroid activity using a reporter gene in the crustacean, Daphnia.2014

    • Author(s)
      Asada, M., Kato, Y., Matsuura, T. and Watanabe, H.
    • Journal Title

      Marine Environmental Research

      Volume: 93 Pages: 118-122

    • DOI

      10.1016/j.marenvres.2013.11.005

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Early Embryonic Expression of a Putative Ecdysteroid-Phosphate Phosphatase in the Water Flea, Daphnia magna (Cladocera: Daphniidae).2014

    • Author(s)
      Asada, M., Kato, Y., Matsuura, T. and Watanabe, H.
    • Journal Title

      Journal of Insect Science

      Volume: 14

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CRISPR/Cas-Mediated Targeted Mutagenesis in Daphnia magna.2014

    • Author(s)
      Nakanishi, T., Kato, Y., Matsuura, T. and Watanabe, H.
    • Journal Title

      Plos One

      Volume: 9

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Optimization of mRNA design for protein expression in the crustacean Daphnia magna.2014

    • Author(s)
      Torner, K., Nakanishi, T., Matsuura, T., Kato, Y. and Watanabe, H.
    • Journal Title

      Molecular Genetics and Genomics

      Volume: 289 Issue: 4 Pages: 707-715

    • DOI

      10.1007/s00438-014-0830-8

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular impact of juvenile hormone agonists on neonatal Daphnia magna.2014

    • Author(s)
      Toyota, K., Kato, Y., Miyakawa, H., Yatsu, R., Mizutani, T., Ogino, Y., Miyagawa, S., Watanabe, H., Nishide, H., Uchiyama, I., Tatarazako, N. and Iguchi, T.
    • Journal Title

      Journal of Applied Toxicology

      Volume: 34 Issue: 5 Pages: 537-544

    • DOI

      10.1002/jat.2922

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In Vitro Membrane Protein Synthesis Inside Cell-Sized Vesicles Reveals the Dependence of Membrane Protein Integration on Vesicle Volume.2013

    • Author(s)
      Soga, H., Fujii, S., Yomo, T., Kato, Y., Watanabe, H. and Matsuura, T.
    • Journal Title

      ACS synthetic biology

      Volume: 0 Issue: 6 Pages: 372-379

    • DOI

      10.1021/sb400094c

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Molecular cloning of doublesex genes of four cladocera (water flea) species2013

    • Author(s)
      Kenji Toyota, Yasuhiko Kato, Masaru Sato, Naomi Sugiura, Shinichi Miyagawa, Hitoshi Miyakawa, Hajime Watanabe, Shigeto Oda, Yukiko Ogino, Chizue Hiruta, Takeshi Mizutani, Norihisa Tatarazako, Susanne Paland, Craig Jackson, John K. Colbourne, Taisen Iguchi
    • Journal Title

      BMC Genomics

      Volume: 14:239 Issue: 1 Pages: 239-239

    • DOI

      10.1186/1471-2164-14-239

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Vesnarinone Suppresses TNF α mRNA Expression by Inhibiting Valosin-containing Protein2013

    • Author(s)
      Hotta K, Nashimoto A, Yasumura E, Suzuki M, Azuma M, Iizumi Y, Shima D, Nabeshima R, Hiramoto M, Okada A, Sakata-Sogawa K, Tokunaga M, Ito T, Ando H, Sakamoto S, Kabe Y, Aizawa S, Imai T, Yamaguchi Y, Watanabe H, Handa H
    • Journal Title

      Mol Pharmacol

      Volume: Vol.83 Issue: 5 Pages: 930-938

    • DOI

      10.1124/mol.112.081935

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Vesnarinone Suppresses TNFα mRNA Expression by Inhibiting Valosin-containing Protein2013

    • Author(s)
      Hotta, K
    • Journal Title

      Molecular Pharmacology

      Volume: 83 Pages: 930-930

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular cloning of doublesex genes of four cladocera (water flea) species2013

    • Author(s)
      Toyota, K
    • Journal Title

      BMC Genomics

      Volume: 14 Pages: 239-239

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genomic integration and germline transmission of plasmid injected into crustacean Daphnia magna eggs.2012

    • Author(s)
      Kato, Y
    • Journal Title

      Plos Genet

      Volume: 7 Pages: 1001345-1001345

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of an RNA interference method in the cladoceran crustacean Daphnia magna2011

    • Author(s)
      Kato, Y.
    • Journal Title

      Dev Genes Evol

      Volume: 220 Pages: 337-345

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Branchiopod Crustacean Daphinia magna : Deep Conservation of a Doublesex Gene in the Sex-Determining Pathway2011

    • Author(s)
      Kato, Y.
    • Journal Title

      Plos Genet

      Volume: 7

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] Development of transgenic Daphnia2015

    • Author(s)
      Yasuhiko KATO, Takashi NAKANISHI, Tomoaki MATSUURA, Hajime WATANABE
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      Hawaii, USA
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] An lncRNA regulates an environmental sex determination in Daphnia magna2015

    • Author(s)
      Yasuhiko Kato, Hajime Watanabe
    • Organizer
      第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
    • Place of Presentation
      神戸国際会議場(兵庫県・神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Efficient DNA integration into germ line genome via TALEN in Daphnia magna2015

    • Author(s)
      Takashi Nakanishi, Yasuhiko Kato, Tomoaki Matsuura, Hajime Watanabe
    • Organizer
      Conference on Transposition and Genome Engineering 2015
    • Place of Presentation
      奈良春日野国際フォーラム(奈良県・奈良市)Nara, Japan
    • Year and Date
      2015-11-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A long noncoding RNA regulates a sex-determining gene doublesex1 in the crustacean Daphnia magna2015

    • Author(s)
      Yasuhiko Kato, Hajime Watanabe
    • Organizer
      EMBL Symposia "Noncoding Genome"
    • Place of Presentation
      Heidelberg, Germany
    • Year and Date
      2015-10-18
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Efficient DNA integration into germ line genome via programmable nucleases in Daphnia magna2015

    • Author(s)
      Takashi Nakanishi, Yasuhiko Kato, Tomoaki Matsuura, Hajime Watanabe
    • Organizer
      Cold Spring Harbor Laboratory Meetings & Courses Program "GENOME ENGINEERING: THE CRISPR/CAS REVOLUTION"
    • Place of Presentation
      Cold Spring Harbor, USA
    • Year and Date
      2015-09-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of transgenic Daphnia for monitoring hormonal activities of chemicals2015

    • Author(s)
      Takashi NAKANISHI, Miki ASADA, Yasuhiko KATO, Hajime WATANABE
    • Organizer
      SETAC Europe 25th Annual Meeting
    • Place of Presentation
      Barcelona, Spain
    • Year and Date
      2015-05-03
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 細胞記憶のメカニズムを理解するためのモデルシステムとしての新生仔期DES処理マウスの膣上皮細胞2014

    • Author(s)
      和田忠士、.北村彩佳、.石川達也、山上修平、.真門剛毅、渡邉肇
    • Organizer
      第37回日本分子生物学会
    • Place of Presentation
      神奈川県横浜市
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] CRISPRノCas9,TALENを利用したオオミジンコのゲノム編集技術の開発2014

    • Author(s)
      中西 貴士・内藤 彰子・加藤 泰彦・松浦 友亮・渡邉 肇
    • Organizer
      第37回日本分子生物学会
    • Place of Presentation
      神奈川県横浜市
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヘテロニ量体型TALENを用いたノックアウトミジンコの作出2014

    • Author(s)
      内藤 彰子・中西 貴士・加藤 泰彦・松浦 友亮・渡邉 肇
    • Organizer
      第37回日本分子生物学会
    • Place of Presentation
      神奈川県横浜市
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 酸化ストレス可視化型Daphniaの創出2014

    • Author(s)
      渡邉 肇
    • Organizer
      環境科学会2014
    • Place of Presentation
      茨城県つくば市
    • Year and Date
      2014-09-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] CRISPR/CasおよびTALENによるDaphnia magnaのゲノム編集技術の開発2014

    • Author(s)
      中西 貴士・内藤 彰子・加藤 泰彦・松浦 友亮・渡邉 肇
    • Organizer
      日本生物工学会
    • Place of Presentation
      北海道札幌市
    • Year and Date
      2014-09-09 – 2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] Evaluation of biological activities of chemical compounds using small organisms2014

    • Author(s)
      渡邉肇
    • Organizer
      新学術班会議
    • Place of Presentation
      愛知県名古屋市
    • Year and Date
      2014-05-28 – 2014-05-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] Establishment of gene knock out technology using TALEN in D.magna.2014

    • Author(s)
      内藤彰子、中西貴士、加藤泰彦、松浦友亮、渡邉肇
    • Organizer
      Daphnia Genomics Consortium MEETING 2014
    • Place of Presentation
      University of Birmingham(イギリス)
    • Related Report
      2013 Annual Research Report
  • [Presentation] The long noncoding RNA DAPALR regulates expression of the sex determining gene doublesex1 in Daphnia magna2014

    • Author(s)
      加藤泰彦
    • Organizer
      Daphnia Genomics Consortium MEETING 2014
    • Place of Presentation
      University of Birmingham(イギリス)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Development of CRISPR/Cas9-mediated genome editing techinique in Daphnia magna2014

    • Author(s)
      中西貴士
    • Organizer
      Daphnia Genomics Consortium MEETING 2014
    • Place of Presentation
      University of Birmingham(イギリス)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ライブイメージングによるミジンコ成長速度定量化方法の確立2013

    • Author(s)
      鈴木安貴子, 加藤泰彦, 松浦友亮, 渡邉肇
    • Organizer
      第40回日本毒性学会学術年会
    • Place of Presentation
      幕張メッセ国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] Comparative Ecotoxicogenomics Using Medaka, Daphnia and Algae2013

    • Author(s)
      渡邉肇
    • Organizer
      ICT Seoul 2013
    • Place of Presentation
      The art Coex conference center
    • Related Report
      2013 Annual Research Report
  • [Presentation] 環境によるオオミジンコの性決定機構2013

    • Author(s)
      加藤泰彦, 渡邉肇
    • Organizer
      生態適応グローバルCOE 生態適応セミナー
    • Place of Presentation
      東北大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 枝角目甲殻類ミジンコの環境性決定を司る幼若ホルモンの作用機構の解析2013

    • Author(s)
      加藤泰彦, 渡邉肇
    • Organizer
      日本進化学会第15回筑波大会
    • Place of Presentation
      筑波大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] Live Imaging of Daphnia magna for Growth Evaluation.2013

    • Author(s)
      鈴木安貴子,渡邉肇
    • Organizer
      第六回日中韓大学院生フォーラム
    • Place of Presentation
      忠南大学(韓国)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Comparative Ecotoxicogenomics Using Medaka, Daphnia and Algae2013

    • Author(s)
      渡邉肇
    • Organizer
      International Environmental Omics Synthesis Conference
    • Place of Presentation
      カーディフ(イギリス)
    • Related Report
      2013 Annual Research Report
  • [Presentation] オオミジンコにおけるエクジステロイト脱リン酸化酵素の機能解析2013

    • Author(s)
      浅田実希, 渡邉肇,加藤泰彦
    • Organizer
      日本動物学会第84回岡山大会
    • Place of Presentation
      岡山大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] Long noncoding RNA transcribed from sex determining gene doulesex1 promoter in the crustacean Daphnia magna.2013

    • Author(s)
      加藤泰彦, 須藤優海,渡邉肇
    • Organizer
      EMBL Symposia "Noncoding genome"
    • Place of Presentation
      European Molecular Biology Laboratory(ドイツ)
    • Related Report
      2013 Annual Research Report
  • [Presentation] リポソーム内部合成膜タンパク質はベシクルサイズ依存的に膜挿入される2013

    • Author(s)
      曽我遥, 加藤泰彦, 渡邉肇,松浦友亮
    • Organizer
      第8回無細胞生命科学研究会
    • Place of Presentation
      新潟大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] リポソーム内無細胞膜タンパク質合成系ヘのトランスロコンの導入2013

    • Author(s)
      太田直樹, 渡邉肇,松浦友亮
    • Organizer
      第8回無細胞生命科学研究会
    • Place of Presentation
      新潟大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] CRISPR/Cas9システムを用いたDaphnia magnaのゲノム編集技術の開発2013

    • Author(s)
      中西貴士, 渡邉肇
    • Organizer
      第3回ゲノム編集研究会
    • Place of Presentation
      広島大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] オオミジンコの性決定遺伝子 doublesex1 のプロモーター領域で発現する長鎖ノンコーディング RNAの機能解析2013

    • Author(s)
      加藤泰彦, 須藤優海,渡邉肇
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸国際会議場他
    • Related Report
      2013 Annual Research Report
  • [Presentation] 環境指標生物Daphnia magnaでのCRISPR/Cas9システムを用いたゲノム編集技術の開発2013

    • Author(s)
      中西貴士, 加藤泰彦, 渡邉肇
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸国際会議場他
    • Related Report
      2013 Annual Research Report
  • [Presentation] 環境指標生物Daphnia magnaにおけるMinosトランスポゾンを用いたゲノム改変技術の開発2012

    • Author(s)
      渡邉 肇
    • Organizer
      第35回日本分子生物学会
    • Place of Presentation
      福岡国際会議場・マリンメッセ
    • Year and Date
      2012-12-11
    • Related Report
      2012 Annual Research Report
  • [Presentation] オオミジンコにおける胚発生初期エクジステロイドの機能解析2012

    • Author(s)
      渡邉 肇
    • Organizer
      第37回日本比較内分泌学会
    • Place of Presentation
      福井大学
    • Year and Date
      2012-11-29
    • Related Report
      2012 Annual Research Report
  • [Presentation] オオミジンコ Daphnia magna における新規レポーターアッセイ系の確立2012

    • Author(s)
      渡邉 肇
    • Organizer
      日本動物学会第83回大会
    • Place of Presentation
      大阪大学
    • Year and Date
      2012-09-14
    • Related Report
      2012 Annual Research Report
  • [Presentation] ミジンコにおける環境依存的な性決定機構2012

    • Author(s)
      渡邉 肇
    • Organizer
      日本進化学会第14回東京大会
    • Place of Presentation
      首都大学
    • Year and Date
      2012-08-21
    • Related Report
      2012 Annual Research Report
  • [Presentation] 酸化ストレス可視化型 Daphnia magnaの創出2012

    • Author(s)
      渡邉 肇
    • Organizer
      環境科学会
    • Place of Presentation
      横浜国立大学
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 環境によるミジンコの性決定機構2011

    • Author(s)
      加藤泰彦, 渡邉肇
    • Organizer
      第13回日本進化学会大会
    • Place of Presentation
      京都(招待講演)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Development of an RNA interference method in the cladoceran crustacean Daphnia magna2011

    • Author(s)
      Kato, Y., Shiga, Y., Tokishita, S-I., Watanabe, H.
    • Organizer
      8th International Congress of Comparative Physiology and Biochemistry
    • Place of Presentation
      Nagoya(招待講演)
    • Related Report
      2011 Annual Research Report
  • [Remarks] 大阪大学大学院工学研究科生命先端工学専攻渡邉研究室

    • URL

      http://www.bio.eng.osaka-u.ac.jp/ez/project/index.html

    • Related Report
      2015 Annual Research Report
  • [Remarks]

    • Related Report
      2013 Annual Research Report

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Published: 2011-08-01   Modified: 2022-02-21  

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