| Budget Amount *help |
¥43,290,000 (Direct Cost: ¥33,300,000、Indirect Cost: ¥9,990,000)
Fiscal Year 2015: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2014: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2013: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2012: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
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| Outline of Final Research Achievements |
Screening programs targeting biological pathways such as TRAIL, Wnt, and Hedgehog signaling and bHLH transcriptional factors resulted in isolation of a variety of bioactive compounds from our natural products collection and natural products based synthetic compounds such as calotropin, a caldenolide with Wnt signal inhibitory activity through enhancement of mRNA expression of casein kinase 1α, artonin E, a prenylated flavone with TRAIL resistance overcoming activity, and vitetrifolin D, a diterpene with hedgehog signal inhibitory activity. Search for Hes1 dimer inhibitors by target protein oriented natural products isolation (TPO-NAPI) using Hes1-beads HPLC system was examined for rapid discovery of natural products with differentiation activating effects of neural stem cells to yield agalloside, a flavonoid glycoside from extract of Aquilaria agallocha (Thymelaeaceae), and inohanamine, a new piperidine alkaloid with a triene side chain, isolated from a Streptomyces strain IFM11584.
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