|Budget Amount *help
¥120,640,000 (Direct Cost: ¥92,800,000、Indirect Cost: ¥27,840,000)
Fiscal Year 2015: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
Fiscal Year 2014: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2013: ¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2012: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2011: ¥50,700,000 (Direct Cost: ¥39,000,000、Indirect Cost: ¥11,700,000)
|Outline of Final Research Achievements
In order to extract the pure deformability characteristics of cells, it is necessary to improve the resolution of cell actuation in the sensing system. This work discussed how to achieve a high speed cell manipulation with high resolution under a micro fluidic chip. A novel concept in this work is the flow reduction mechanism, where a flow is generated by a macro actuator placed outside of a micro fluidic chip. The flow can be drastically reduced at the cell manipulation point in a micro channel due to the elasticity embedded into the micro fluidic chip. By using this advantage, we could construct a high resolution cell manipulation system and successfully achieved the cell positioning based on a visual feedback control with a resolution of 240nm, together with the maximum frequency of 130Hz.
As an application example, we showed a new concept of cell fatigue evaluation where we continuously impart mechanical stress to the cell until it eventually get a damage.