|Budget Amount *help
¥89,700,000 (Direct Cost: ¥69,000,000、Indirect Cost: ¥20,700,000)
Fiscal Year 2015: ¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000)
Fiscal Year 2014: ¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2013: ¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2012: ¥25,090,000 (Direct Cost: ¥19,300,000、Indirect Cost: ¥5,790,000)
Fiscal Year 2011: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
|Outline of Final Research Achievements
We took in vivo and in vitro images of single molecules and particles in mice and culture cells to understand the function of molecules. In vivo imaging, we images neutrophil labeled with quantum dots and cancer cells expressing tubulin-GFP in mouse auricles by spinning confocal microscope. The individual cancer cells, neutrophils and vesicles transporting in the neutrophil in mice were clear observed even at high spatiotemporal resolution of ~10ms and ~10nm. The speed of vesicle transport was much higher than that in purified cells.
To understand the damage of cancer cells quantitatively for cancer therapy, we took the phase contrast images of cancer cells and analyzed the intensity fluctuation (standard deviation of intensity) of each pixels in images. The magnitude of the fluctuation decreased with progress of cell damages. Long term observation of the fluctuation is available to detect the cell damage.