|Budget Amount *help
¥88,140,000 (Direct Cost: ¥67,800,000、Indirect Cost: ¥20,340,000)
Fiscal Year 2015: ¥12,090,000 (Direct Cost: ¥9,300,000、Indirect Cost: ¥2,790,000)
Fiscal Year 2014: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2013: ¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2012: ¥36,660,000 (Direct Cost: ¥28,200,000、Indirect Cost: ¥8,460,000)
Fiscal Year 2011: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
|Outline of Final Research Achievements
In living cells, there are many huge filamentous structures, organelles, protein complexes, and nucleic acids. The complex intracellular environment rises from awful molecular crowding conditions in the cytosol. To learn the dynamic molecular reactions in living cells, it is essential to clarify their physicochemical structure and features like disproportional macromolecular crowding structures, molecular diffusion, and excluded volume effect. In this project, we have aimed to analyze the macromolecular dynamics, simulate physical structures.
To analyze the dynamics of mRNA in living cells, we have to develop an mRNA detection system with nM sensitivity in molecular crowding conditions. We modified the nanoneedle with molecular beacon, which is a highly sensitive nuclear acid probe. The developed nanoprobe could detect as small as 1 nM RNA in solution. By using the nanoprobe for GAPDH or beta-actin, we succeeded in selective detection of the mRNA inside the cells.