Clarification of molecular mechanism of lymphatic metastasis and development of new therapy for cancer metastasis

• Project Area: Molecular Science for Nanomedicine
• Project/Area Number: 23107009
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Tohoku University
• Principal Investigator: Gonda Kohsuke 東北大学, 医学(系)研究科(研究院), 教授 (80375435)
• Project Period (FY): 2011-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥105,170,000 (Direct Cost: ¥80,900,000、Indirect Cost: ¥24,270,000); Fiscal Year 2015: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2014: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2013: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000); Fiscal Year 2012: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2011: ¥31,720,000 (Direct Cost: ¥24,400,000、Indirect Cost: ¥7,320,000)
• Keywords: ナノメディシン / がん / 転移 / ナノ粒子 / 蛍光 / Ｘ線 / イメージング / ナノバイオ / バイオテクノロジー / リンパ節 / 予後 / 診断 / ナノ材料 / 癌 / 生物物理

Outline of Final Research Achievements

Diagnosis of lymph node metastasis is very important for improving cancer prognosis because cancer metastasis tend to occur via lymph vessels rather than blood vessels. To clarify molecular mechanism of lymphatic metastasis and apply the mechanism to development of new diagnostic method for cancer metastasis, we mainly performed three research projects as follows: (1) elucidation of mechanism for lymph node metastasis using unique tumor-bearing mice, (2) development of detection method for lymphatic metastasis using various imaging modalities, and (3) development of pathological diagnosis of surgically-removed primary tumor and metastatic lymph nodes.

Research Products

• [Journal Article] Fabrication of gadolinium hydroxide nanoparticles usingion-exchange resin and their MRI property. (2016)
  • Author(s): Kobayashi Y, Morimoto H, Nakagawa T, Kubota Y, Gonda K, Ohuchi N.
  • Journal Title: Journal of Asian Ceramic Societies Volume: 4 Issue: 1 Pages: 138-142
  • DOI: 10.1016/j.jascer.2016.01.005
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Stabilization of Silica-Coated Silver Iodide Nanoparticles. (2016)
  • Author(s): Kobayashi Y, Ayame T, Shibuya K, Nakagawa T, Kubota Y, Gonda K, Ohuchi N.
  • Journal Title: Pigment & Resin Technology Volume: 45 Issue: 2 Pages: 99-105
  • DOI: 10.1108/prt-10-2014-0083
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Fabrication of quantum dot/silica core-shell particles immobilizing Au nanoparticles and their dual imaging functions. (2016)
  • Author(s): Kobayashi Y, Matsudo H, Li T, Shibuya K, Kubota Y, Oikawa T, Nakagawa T, Gonda K.
  • Journal Title: Applied Nanoscience Volume: 6 Issue: 3 Pages: 301-307
  • DOI: 10.1007/s13204-015-0440-8
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Preparation of high-concentration colloidal solution of silica-coated gold nanoparticles and their application to X-ray imaging. (2016)
  • Author(s): Kobayashi Y, Shibuya K, Tokunaga M, Kubota Y, Oikawa T, and Gonda K
  • Journal Title: Journal of Sol-Gel Science and Technology Volume: 78 Issue: 1 Pages: 82-90
  • DOI: 10.1007/s10971-015-3921-z
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Predictive diagnosis of the risk of breast cancer recurrence after surgery by single-particle quantum dot imaging. (2015)
  • Author(s): Gonda K, Miyashita M, Higuchi H, Tada H, Watanabe TM, Watanabe M, Ishida T, Ohuchi N.
  • Journal Title: Scientific Report. Volume: 22(5) Issue: 1 Pages: 14322-14322
  • DOI: 10.1038/srep14322
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Highly Sensitive Imaging of Cancer with Functional Nanoparticles (2015)
  • Author(s): Gonda K, Hamada Y, Kitamura N, Tada H, Miyashita M, Kamei T, Ishida T, Ohuchi N.
  • Journal Title: Journal of Photopolymer Science and Technology Volume: 28 Issue: 6 Pages: 731-736
  • DOI: 10.2494/photopolymer.28.731
  • NAID: 130005108630
  • ISSN: 0914-9244, 1349-6336
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Preparation of Au/silica/poly(ethylene glycol) nanoparticle colloid solution and its use in x-ray imaging process. (2015)
  • Author(s): Kobayashi Y, Nagasu R, Nakagawa T, Kubota Y, Gonda K, Ohuchi N.
  • Journal Title: Nanocomposites Volume: 2 Issue: 2 Pages: 83-88
  • DOI: 10.1179/2055033215y.0000000003
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Preparation of Silica-Coated Quantum Dot Nanoparticle Colloid Solutions and Their Application in <i>in-vivo</i> Fluorescence Imaging (2015)
  • Author(s): Kobayashi Y, Matsudo H, Kubota Y, Nakagawa T, Gonda K, Ohuchi N.
  • Journal Title: JOURNAL OF CHEMICAL ENGINEERING OF JAPAN Volume: 48 Issue: 2 Pages: 112-117
  • DOI: 10.1252/jcej.14we218
  • NAID: 130004862051
  • ISSN: 0021-9592, 1881-1299
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Preparation of Gd Complex-Immobilized Silica Particles and Their Application to MRI. (2013)
  • Author(s): Kobayashi Y, Morimoto H, Nakagawa T, Kubota Y, Gonda K, Ohuchi N.
  • Journal Title: ISRN Nanotechnology 2013 Volume: 1 Pages: 1-6
  • Peer Reviewed
• [Journal Article] In-vivo fluorescence imaging technique using colloid solution of multiple quantum dots/silica/poly(ethylene glycol) nanoparticle. (2013)
  • Author(s): Kobayashi Y, Matsudo H, Nakagawa T, Kubota Y, Gonda K, Ohuchi N.
  • Journal Title: Journal of Sol-Gel Science and Technology Volume: 66 Pages: 31-37
  • Peer Reviewed
• [Journal Article] Preparation of AgI/silica/poly(ethylene glycol) nanoparticle colloid solution and X-ray imaging using it. (2013)
  • Author(s): Kobayashi Y, Ayame T, Nakagawa T, Kubota Y, Gonda K and Ohuchi N.
  • Journal Title: ISRN Nanomaterials 2013 Volume: 1 Pages: 1-5
  • Peer Reviewed
• [Journal Article] Fabrication of hollow particles composed of silica containing gadolinium compound and magnetic resonance imaging using them. (2013)
  • Author(s): Kobayashi Y, Morimoto H, Nakagawa T, Kubota Y, Gonda K, Ohuchi N.
  • Journal Title: Journal of Nanostructure in Chemistry Volume: 3 Pages: 62-62
  • Peer Reviewed
• [Journal Article] Single quantum dot tracking reveals that an individual multivalent 2 HIV-1 Tat-protein transduction domain can activate machinery for 3 lateral transport and endocytosis (2013)
  • Author(s): 3.Yasuhiro Suzuki*, Chandra Nath Roy, Warunya Promjunyaku, Hiroyasu Hatakeyama, Kohsuke Gonda, Junji Imamura, Biju Vasudevan Pillai, Noriaki Ohuchi, Makoto Kanzaki , Hideo Higuchi, and Mitsuo Kaku
  • Journal Title: Mol. Cell Biol. Volume: 33 Issue: 15 Pages: 3039-3049
  • DOI: 10.1128/mcb.01717-12
  • Peer Reviewed / Open Access
• [Journal Article] Preparation of silica-c0ated gadolinium compound particle colloid solution and its application in imaging. (2013)
  • Author(s): Kobayashi Y, Morimoto H, Nakagawa T, Gonda K, Ohuchi N.
  • Journal Title: Advanced in Nano Research Volume: 1 Pages: 159-160
  • Peer Reviewed
• [Journal Article] X-ray imaging technique using colloid solution of Au/silica/poly(ethylene glycol) nanoparticles. (2013)
  • Author(s): Kobayashi Y, Inose H, Nagasu R, Nakagawa T, Kubota Y, Gonda K, Ohuchi N.
  • Journal Title: Material Research Innvations Volume: 17 Pages: 507-514
• [Journal Article] Development of a quantitative diagnostic method of estrogen receptor expression levels by immunohistochemistry using organic fluorescent material-assembled nanoparticles (2012)
  • Author(s): Gonda K, Miyashita M, Watanabe M, Takahashi Y, Goda H, Okada H, Nakano Y, Tada H, Amari M, Ohuchi N.
  • Journal Title: Biochem Biophys Res Commun Volume: 426(3) Issue: 3 Pages: 409-414
  • DOI: 10.1016/j.bbrc.2012.08.105
  • Peer Reviewed
• [Journal Article] Synthesis of Au-silica core-shell particles by sol-gel process (2012)
  • Author(s): Kobayashi Y, Inose H, Nakagawa T, Gonda K, Takeda M, Ohuhi N, Kasuya A.
  • Journal Title: Surface Engineering Volume: 28(2) Issue: 2 Pages: 129-133
  • DOI: 10.1179/1743294411y.0000000069
  • Peer Reviewed
• [Journal Article] Synthesis of high concentration colloid solution of silica-coated AgI nanoparticles (2012)
  • Author(s): Kobayashi Y, Minato M, Ihara K, Nakagawa T, Gonda K, Takeda M, Ohuchi N, Kasuya A.
  • Journal Title: J Nanosci Nanotechnol Volume: 12(8) Issue: 8 Pages: 6741-6745
  • DOI: 10.1166/jnn.2012.4531
  • Peer Reviewed
• [Journal Article] Multilayered, core/shell nanoprobes based on magnetic ferric oxide particles and quantum dots for multimodality imaging of breast cancer tumors. (2012)
  • Author(s): Qiang Ma, et al.
  • Journal Title: Biomaterials Volume: 33 Issue: 33 Pages: 8486-8494
  • DOI: 10.1016/j.biomaterials.2012.07.051
  • Peer Reviewed
• [Journal Article] Development of Silica-Coated Silver Iodide Nanoparticles and Their Biodistribution (2012)
  • Author(s): Sakurai Y, Tada H, Gonda K, Takeda M, Cong L, Amari M, Kobayashi Y, Watanabe M, Ishida T, Ohuchi N.
  • Journal Title: The Tohoku Journal of Experimental Medicine Volume: 228 Issue: 4 Pages: 317-323
  • DOI: 10.1620/tjem.228.317
  • NAID: 130004460030
  • ISSN: 0040-8727, 1349-3329
  • Peer Reviewed
• [Journal Article] X-ray imaging technique using colloid solution of AgI/silica/poly(ethylene glycol) nanoparticles. (2012)
  • Author(s): Yoshio Kobayashi
  • Journal Title: Materials Focus Volume: 1 Pages: 127-130
  • Peer Reviewed
• [Journal Article] Fabrication andfluorescence properties of multilayered core-shell particles composed of quantum dot, gadolinium compound, and silica (2012)
  • Author(s): Kobayashi Y, Nozawa T, Nakagawa T, GondaK, Takeda M, Ohuchi N.
  • Journal Title: J Mater Sci Volume: 47 Issue: 4 Pages: 1852-1859
  • DOI: 10.1007/s10853-011-5972-z
  • Peer Reviewed
• [Journal Article] In vivo imaging of the molecular distribution of the VEGF receptor during angiogenesis in a mouse model of ischemia (2011)
  • Author(s): Hamada Y, Gonda K, Takeda M, Sato A, Watanabe M, Yambe T, Satomi S, Ohuchi N
  • Journal Title: Blood Volume: 118 Issue: 13 Pages: e93-e100
  • DOI: 10.1182/blood-2010-12-322842
  • Peer Reviewed
• [Journal Article] ナノイメージングで探るがん転移の仕組み (2011)
  • Author(s): 権田幸祐、樋口秀男、渡邉朋信、武田元博、大内憲明
  • Journal Title: SURGERY FRONTIER Volume: 18 Pages: 50-57
• [Journal Article] 見つけることに意義がある-1分子計測の可能性- (2011)
  • Author(s): 上村想太郎、小澤岳昌、加地範匡、権田幸祐
  • Journal Title: 現代化学 Volume: 11月号 Pages: 26-30
• [Journal Article] 血管新生における血管内皮増殖因子受容体分布の生体分子イメージング (2011)
  • Author(s): 濱田庸、権田幸祐、佐藤成、山家智之、里見進、大内憲明
  • Journal Title: ナノ学会会報 Volume: 10 Pages: 35-39
  • NAID: 40019055637
• [Presentation] Prognostic Diagnosis of Breast Cancer with Immunohistochemistry by Single-Particle Quantum Dot Imaging. (2015)
  • Author(s): Gonda K Ohuchi N.
  • Organizer: 9th International Symposium on Nanomedicine
  • Place of Presentation: 三重大学(三重県津市)
  • Year and Date: 2015-12-11
  • Invited
• [Presentation] Highly sensitive imaging of cancer with functional nanoparticles. (2015)
  • Author(s): Gonda K
  • Organizer: 32nd International Conference of Photopolymer Science and Technology.
  • Place of Presentation: Makuhari Messe, Chiba
  • Year and Date: 2015-06-24
  • Int'l Joint Research / Invited
• [Presentation] 蛍光ナノ粒子の１粒子イメージングによる定量的免疫組織化学法の技術開発 (2015)
  • Author(s): 権田幸祐
  • Organizer: 第104回日本病理学会総会
  • Place of Presentation: 名古屋国際会議場(愛知県名古屋市)
  • Year and Date: 2015-05-01
  • Invited
• [Presentation] Development of cancer nano-imaging for nanomedicine. (2013)
  • Author(s): Kohsuke Gonda
  • Organizer: International Conference on Biomaterials Science 2013
  • Place of Presentation: Tsukuba
  • Year and Date: 2013-03-19
• [Presentation] 金ナノ粒子を用いた腫瘍のCTイメージング (2013)
  • Author(s): 中川智彦、権田幸祐、亀井尚、久保田洋介、叢莉蔓、中野寧、大内憲明
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
• [Presentation] High Accuracy Imaging of Cancer and Peripheral Artery Disease with X-ray CT and Fluorescence. (2013)
  • Author(s): Gonda K, Kobayashi Y, Ohuchi N.
  • Organizer: 7th International Symposium on Nanomedicine
  • Place of Presentation: 九州工業大学
  • Invited
• [Presentation] High Resolution Imaging of Angiogenesis in Hind Limb Ischemic Model Mouse with Nanoparticles. (2013)
  • Author(s): Kawamura K, Gonda K, Hamada Y, Kubota Y, Ohuchi N.
  • Organizer: 7th International Symposium on Nanomedicine
  • Place of Presentation: 九州工業大学
• [Presentation] In Vivo Dual Imaging of Lymph Node Using X-ray CT and Fluorescence. (2013)
  • Author(s): Kubota Y, Gonda K, Kobayashi Y, Kamei T, Nakagawa T, Matsudo, Shibuya K, Ohuchi N
  • Organizer: 7th International Symposium on Nanomedicine
  • Place of Presentation: 九州工業大学
• [Presentation] Development of imaging technology for advanced cancer diagnosis. (2012)
  • Author(s): Kohsuke Gonda
  • Organizer: 6th International Symposium on Nanomedicine
  • Place of Presentation: Matsue
  • Year and Date: 2012-11-29
  • Invited
• [Presentation] Nano-bio imaging of disease mechanisms with fluorescent nano-particles. (2012)
  • Author(s): Kohsuke Gonda
  • Organizer: 2012 Northeastern Asian Symposium
  • Place of Presentation: Sendai
  • Year and Date: 2012-09-19
  • Invited
• [Presentation] 高精度蛍光ナノイメージングで拓くがん医学・医療研究の新展開 (2012)
  • Author(s): 権田幸祐
  • Organizer: 日本バイオマテリアル学会シンポジウム2012
  • Place of Presentation: 仙台
  • Invited
• [Presentation] Development of imaging system for advanced nanomedicine (2012)
  • Author(s): Gonda K, Hikage M, Hamada Y, Nakagawa T, Ohuchi N
  • Organizer: 5th International Symposium on Nanomedicine
  • Place of Presentation: Nagoya
• [Presentation] In vivo molecular imaging of cancer metastasis and angiogenesis in mice using fluorescent nano-particle (2011)
  • Author(s): Gonda K
  • Organizer: 3^<rd> Global COE International Symposium "New Trends in Basic and Clinical Cancer Research for Innovative Therapy"
  • Place of Presentation: Nagoya
• [Book] 先端バイオマテリアルハンドブック (2012)
  • Author(s): 樋口秀男
  • Publisher: NTS出版
• [Book] ナノ学会会報 (2012)
  • Author(s): 中川智彦
  • Publisher: ナノ学会
• [Book] ここまで進んだバイオセンシング・イメージング Part II 研究最前線 (2012)
  • Author(s): 多田寛
  • Publisher: 化学同人
• [Remarks] 東北大学大学院医学系研究科 ナノ医科学講座
  • URL: http://www.med.tohoku.ac.jp/org/contribute/107/index.html