| Budget Amount *help |
¥99,710,000 (Direct Cost: ¥76,700,000、Indirect Cost: ¥23,010,000)
Fiscal Year 2015: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
Fiscal Year 2014: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2013: ¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000)
Fiscal Year 2012: ¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2011: ¥41,600,000 (Direct Cost: ¥32,000,000、Indirect Cost: ¥9,600,000)
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| Outline of Final Research Achievements |
Various serine/threonine kinases are activated in cells upon numerous extracellular stresses, such as heat, hypoxia and oxidation. These stresses activate four different types of kinases, EIF2AK1, EIF2AK2, EIF2AK3, EIF2AK4, which consequently promote phosphorylation of eIF2α at Ser51. Our proteomics analysis of SG components revealed UBAP2L is a critical component of SG. UBAP2L contains ubiquitin-associated domain and RGG motif. UBAP2L is localized to SGs when cells are treated with heat, arsenite, hydrogen peroxide, and sorbitol. Fluorescence recovery after photobleaching (FRAP) analysis revealed that UBAP2L localization to the SG is dynamic. UBAP2L depletion by siRNA transfection showed that UBAP2L was critical for the organization of SG. Formation of SG is associated with cell survival in the presence of extracellular stresses. Further analysis of UBAP2L and UBAP2 functions in RNA processing and SG formation may give insight for the pathogenesis of neurodegenerative diseases.
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