Population genomics of plant host-pathogen interactions

• Project Area: Correlative gene system: establishing next-generation genetics
• Project/Area Number: 23113009
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Iwate Biotechnology Research Center
• Principal Investigator: Terauchi Ryohei 公益財団法人岩手生物工学研究センター, その他部局等, その他 (50236981)
• Co-Investigator(Kenkyū-buntansha): SAITOH Hiromasa (公財)岩手生物工学研究センター, ゲノム育種研究部, 主任研究員 (20414336) ; 吉田 健太郎 (財)岩手生物工学研究センター, 生命科学研究部, 研究員 (40570750)
• Project Period (FY): 2011-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥108,810,000 (Direct Cost: ¥83,700,000、Indirect Cost: ¥25,110,000); Fiscal Year 2015: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2014: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2013: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000); Fiscal Year 2012: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000); Fiscal Year 2011: ¥33,020,000 (Direct Cost: ¥25,400,000、Indirect Cost: ¥7,620,000)
• Keywords: 共進化 / ゲノム解析 / イネ / いもち病菌 / 植物 / 病原菌 / 自然選択 / 集団遺伝学 / 全ゲノム解析 / シーケンス / 耐病性

Outline of Final Research Achievements

Strong reciprocal natural selection between a host plant and a pathogen leaves DNA signatures on the genomes of respective organisms. By employing whole genome sequencing and detecting DNA signatures of selection, we succeeded in isolating two rice resistance (R-) genes (Pia and Pii) and three Magnaporthe oryzae avirulence effector (AVR) genes (AVR-Pia, AVR-Pii, AVR-Pik). Molecular interactions between rice R-protein Pik and Magnaporthe AVR-Pik revealed that their genes are coevolving. We also have developed a series of methods including MutMap and QTL-seq to quickly detect selection signature by whole genome resequencing.

Research Products

• [Int'l Joint Research] The Sainsbury Laboratory/John Innes Centre(英国)
• [Int'l Joint Research] Frankfurt University(Germany)
• [Int'l Joint Research] INRA(France)
• [Journal Article] A chloroplast-localized protein LESION AND LAMINA BENDING affects defense and growth responses in rice. (2016)
  • Author(s): Tamiru, M., Takagi, H., Abe, A., Yokota, T., Kanzaki, H., Okamoto, H., Saitoh, H., Takahashi, H., Fujisaki, K., Oikawa, K., Uemura, A., Natsume, S., Jikumaru, Y., Matsuura, H., Umemura, K., Terry, M. J., Terauchi, R.
  • Journal Title: New Phytologist Volume: 210 Issue: 4 Pages: 1282-1297
  • DOI: 10.1111/nph.13864
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Whole genome sequencing approaches to understand Magnaporthe-rice interactions. (2016)
  • Author(s): Terauchi, R., Kanzaki, H., Fuijsaki, K., Takagi, H., Abe, A., Yoshida, K., Okuyama, Y., Tamiru, M., Saitoh, H.
  • Journal Title: Physiological and Molecular Plant Pathology Volume: 94 Pages: 1-5
  • DOI: 10.1016/j.pmpp.2016.03.007
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] WIPPER: an accurate and efficient field phenotyping platform for large-scale applications (2015)
  • Author(s): Utsushi, H., Abe, A., Tamiru, M., Ogasawara, Y., Obara, T., Sato, E., Ochiai, Y., Terauchi, R., Takagi, H.
  • Journal Title: Breeding Science Volume: 65 Issue: 3 Pages: 285-289
  • DOI: 10.1270/jsbbs.65.285
  • NAID: 130005088162
  • ISSN: 1344-7610, 1347-3735
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Rice Exo70 interacts with a fungal effector, AVR-Pii and is required for AVR-Pii-triggered immunity (2015)
  • Author(s): Fujisaki, K., Abe, Y., Ito, A., Saitoh, H., Yoshida, K., Kanzaki H., Kanzaki, E., Utsushi, H., Yamashita, T., Kamoun, S. and Terauchi, R.
  • Journal Title: The Plant Journal Volume: 83 Issue: 5 Pages: 875-887
  • DOI: 10.1111/tpj.12934
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Structural basis of pathogen recognition by an integrated HMA domain in a plant NLR immune receptor. (2015)
  • Author(s): Maqbool, A., Saitoh, H., Franceschetti, M., Stevenson, C.E.M., Uemura, A., Kanzakii, H., Kamoun, S., Terauchi, R., Banfield, M. J.
  • Journal Title: eLife Volume: 4 Pages: 1-24
  • DOI: 10.7554/elife.08709
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] A cytochromoe P450, OsDSS1, is involved in growth and drought stress responses in rice (Oryza saliva L.) (2015)
  • Author(s): Tamiru, M., Undan, J. R., Takagi, H., Abe, A., Yoshida, K., Undan, J. Q., Natsume, S., Uemura, A., Saitoh, H., Matsumura, H., Urasaki, N., Yokota, T., Terauchi, R.
  • Journal Title: Plant Molecular Biology Volume: 88 Issue: 1-2 Pages: 85-99
  • DOI: 10.1007/s11103-015-0310-5
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] MutMap accelerates breeding of a salt-tolerant rice cultivar (2015)
  • Author(s): Takagi, H., Tamiru, M., Abe, A., Yoshida, K., Uemura, A., Yaegashi, H., Obara, T., Oikawa, K., Utsushi, H., Kanzaki, E., Mitsuoka, C., Natsume, S., Kosugi, S., Kanzaki, H., Matsumura, H., Urasaki, N., Kamoun, S., Terauchi, R.
  • Journal Title: Nature Biotechnology Volume: 33 Issue: 5 Pages: 445-449
  • DOI: 10.1038/nbt.3188
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Harvesting the promising fruits of genomics: applying genome sequencing technologies to crop breeding (2014)
  • Author(s): Varshney, R. K., Terauchi, R., McCouch, S. R.
  • Journal Title: PLoS Biology Volume: 12 Issue: 6 Pages: 1-7
  • DOI: 10.1371/journal.pbio.1001883
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The NB-LRR proteins RGA4 and RGA5 interact functionally and physically to confer disease resistance (2014)
  • Author(s): Cesari, S., Kanzaki, H., Fujiwara, T., Bernoux, M., Chalvon, V., Kawano, Y., Shimamoto, K., Dodds, P., Terauchi, R., Kroj, T.
  • Journal Title: EMBO Journal Volume: 33 Issue: 17 Pages: 1941-1959
  • DOI: 10.15252/embj.201487923
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The rice (Oryza saliva L.) LESION MIMIC RESEMBLING, which encodes an AAA-type ATPase, is implicated in defense response (2014)
  • Author(s): Fekih, R., Tamiru, M., Kanzaki, H., Abe, A., Yoshida, K., Kanzaki, E., Saitoh, H., Takagi, H., Natsume, S., Undan, J. R., Undan, J, Terauchi, R.
  • Journal Title: Molecular Genetics and Genomics Volume: 290 Issue: 2 Pages: 611-622
  • DOI: 10.1007/s00438-014-0944-z
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The tillering phenotype of the rice plastid terminal oxidase (PTOX) loss-of-function mutant is associated with strigolactone deficiency (2014)
  • Author(s): Tamiru,M., Abe,A., Utsushi,H., Yoshida,K., Takagi,H., Fujisaki,K., Undan,J.R., Rakshit,S., Takaichi,S., Jikumaru,Y., Yokota,T., Terry,M.J., Terauchi,R.
  • Journal Title: New Phytologist Volume: 202 Issue: 1 Pages: 116-131
  • DOI: 10.1111/nph.12630
  • Peer Reviewed
• [Journal Article] MoST1 encoding a hexose transporter-like protein is involved in both conidiation and mycelial melanization of Magnaporthe oryzae. (2014)
  • Author(s): Saitoh, H., HIrabuchi, A., Fujisawa, S., Mitsuoka, C., Terauchi, R. Takano, Y.
  • Journal Title: FEMS Microbiology Letters Volume: 352 Issue: 1 Pages: 104-113
  • DOI: 10.1111/1574-6968.12369
  • Peer Reviewed
• [Journal Article] Two distinct secretion systems facilitate tissue invasion by the rice blast fungus Magnaporthe oryzae. (2013)
  • Author(s): Giraldo, M.C., Dagdas, Y.F., Gupta, Y.K., Mentlak, T.A., Yi, M., Martinez-Rocha, A.L., Saitoh, H., Terauchi, R., Talbot, N.J., Valent, B.
  • Journal Title: Nature Communications Volume: 4 Issue: 1 Pages: 1996-1996
  • DOI: 10.1038/ncomms2996
  • Peer Reviewed
• [Journal Article] MutMap-Gap:whole-genome resequencing of mutant F2 progeny bulk combined with de novo assembly of gap regions identifies the rice blast resistance gne Pii (2013)
  • Author(s): Takagi,H., Uemura,A., Yaegashi,H., Tamiru, M., Abe, A., Mitsuoka,C., Utsushi,H., Natsume,S., Kanzaki,H., Matsumura,H., Saitoh,H., Yoshida,K., Cano,L.M., Kamoun,S., Terauchi,R.
  • Journal Title: New Phytologist Volume: 200 Issue: 1 Pages: 276-283
  • DOI: 10.1111/nph.12369
  • Peer Reviewed
• [Journal Article] MutMap+ : Genetic mapping and mutant identification without crossing in rice (2013)
  • Author(s): Fekih, R., Takagi, H., Tamiru, M., Abe, A., Natsume, S., et al.
  • Journal Title: PLoS One Volume: 8 Issue: 7 Pages: e68529-e68529
  • DOI: 10.1371/journal.pone.0068529
  • Peer Reviewed
• [Journal Article] (2013)
  • Author(s): Shunichi Kosugi, Satoshi Natsume, Kentaro Yoshida, Daniel MacLean, Liliana Cano, Sophien Kamoun, and Ryohei Terauchi
  • Journal Title: PLoS One Volume: 8 Issue: 10 Pages: e75402-e75402
  • DOI: 10.1371/journal.pone.0075402
  • Peer Reviewed
• [Journal Article] QTL-seq: rapid mapping of quantitative trait loci in rice by whole genome resequencing of DNA from two bulked populations (2013)
  • Author(s): TAKAGI, H.
  • Journal Title: The Plant Journal Volume: 74 Issue: 1 Pages: 174-183
  • DOI: 10.1111/tpj.12105
  • Peer Reviewed
• [Journal Article] Deployment of the Burkholderia glumae type III secretion system as an efficient tool for translocating pathogen effectors to monocot cells. (2013)
  • Author(s): Sharma S, Sharma S, Hirabuchi A, Yoshida K, Fujisaki K, Ito A, Uemura A, Terauchi R, Kamoun S, Sohn KH, Jones JDG, Saitoh H.
  • Journal Title: The Plant Journal Volume: 74 Issue: 4 Pages: 701-712
  • DOI: 10.1111/tpj.12148
  • Peer Reviewed
• [Journal Article] Genome sequencing reveals agronomically important loci in rice using MutMap (2013)
  • Author(s): Abe Akira, Kosugi Shunichi, Yoshida Kentaro, Natsume Satoshi, Takagi Hiroki, Kanzaki Hiroyuki, Matsumura Hideo, (他 7 名 , 7 番 目)
  • Journal Title: Nature Biotechnology Volume: 30 Issue: 2 Pages: 174-178
  • DOI: 10.1038/nbt.2095
  • Peer Reviewed
• [Journal Article] Mutation in <i>OsLMS</i>, a gene encoding a protein with two double-stranded RNA binding motifs, causes lesion mimic phenotype and early senescence in rice (<i>Oryza sativa</i> L.) (2012)
  • Author(s): Undan JR, Tamiru M, Abe A, Yoshida K, Kosugi S, Takagi H, Yoshida K, Kanzaki H, Saitoh H, Fekih R, Sharma S, Undan J, Yano M, Terauchi R.
  • Journal Title: Genes & Genetic Systems Volume: 87 Issue: 3 Pages: 169-179
  • DOI: 10.1266/ggs.87.169
  • NAID: 10030945208
  • ISSN: 1341-7568, 1880-5779
  • Peer Reviewed
• [Journal Article] Whole genome sequencing and future breeding of rice. (2012)
  • Author(s): Terauchi, R., Abe, A., Takagi, H., Yoshida, K., Kosugi, S., Natsume, S., Yaegashi, H, Kanzaki, H., Matsumura, H., Mitsuoka, C., Utsushi, H., Tamiru, M.
  • Journal Title: J. Plant Biochem. Biotechnol. Volume: 21 Issue: S1 Pages: 10-14
  • DOI: 10.1007/s13562-012-0133-2
  • Peer Reviewed
• [Journal Article] Arms race co-evolution of Magnaporthe oryzae AVR-Pik and rice Pik genes driven by their physical interactions. (2012)
  • Author(s): Kanzaki H, Yoshida K, Saitoh H, Fujisaki K, Hirabuchi A, Alaux L, Fournier E, Tharreau D, Terauchi R.
  • Journal Title: The Plant Journal Volume: 72 Issue: 6 Pages: 894-907
  • DOI: 10.1111/j.1365-313x.2012.05110.x
  • Peer Reviewed
• [Presentation] Towards understanding molecular co-evolution of Magnaporthe and rice (2015)
  • Author(s): Terauchi R.
  • Organizer: The 11th US-Japan Scientific Seminar
  • Place of Presentation: Kagawa International Hall, Takamatsu, Japan
  • Year and Date: 2015-10-25
  • Int'l Joint Research / Invited
• [Presentation] Genome sequencing and modern breeding (2015)
  • Author(s): Terauchi R.
  • Organizer: 20th Japanese-German Symposium Agriculture and Food Supply Challenges and Perspectives
  • Place of Presentation: Potsdam University, Germany
  • Year and Date: 2015-05-08
  • Int'l Joint Research / Invited
• [Presentation] Interaction between rice NLR Pik and Magnaporthe oryzae effector AVR-Pik (2015)
  • Author(s): Terauchi, R.
  • Organizer: NLR workshop at Schloss Ringberg
  • Place of Presentation: Tegern see, Germany
  • Year and Date: 2015-05-03
  • Int'l Joint Research / Invited
• [Presentation] Magnaporthe-rice interactions as revealed by whole genome analysis (2014)
  • Author(s): Terauchi R.
  • Organizer: IS-MPMI XIV International Congress
  • Place of Presentation: Rhodos, Greece
  • Year and Date: 2014-07-07 – 2014-07-10
  • Invited
• [Presentation] いもち病菌とイネ相互作用の解析 (2013)
  • Author(s): 寺内良平
  • Organizer: 第５４回日本植物生理学会年会
  • Place of Presentation: 岡山大学
  • Invited
• [Presentation] イネもみ枯細菌病III型分泌機構を利用したイネいもち病菌エフェクターの単子葉植物細胞内への移行 (2013)
  • Author(s): 齋藤宏昌
  • Organizer: 平成２５年度日本植物病理学会大会
  • Place of Presentation: 岐阜大学
• [Presentation] Toward understanding Magnaporthe-rice interactions (2012)
  • Author(s): TERAUCHI, R.
  • Organizer: 30th New Phytologist Symposium: Immunomodulation by plant-associated organisms
  • Place of Presentation: Fallen Leaf Lake, California, USA
  • Year and Date: 2012-09-17
  • Invited
• [Presentation] Toward understanding Magnaporthe-rice interactions (2012)
  • Author(s): TERAUCHI, R.
  • Organizer: 15th International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2012-07-30
  • Invited
• [Presentation] Genomics reveals Magnaporthe-rice interactions (2012)
  • Author(s): 寺内良平
  • Organizer: 9emes Rencontres de Phytopathologie-Mycologie de la Societe Francaise de Phytopathologie : Journees Jean Chevaugeon 2012
  • Place of Presentation: Aussois, France
  • Year and Date: 2012-01-17
• [Presentation] 全ゲノム情報を活用したイネ－いもち病菌相互作用の解析 (2012)
  • Author(s): 寺内良平
  • Organizer: 平成２４年度植物感染生理談話会
  • Place of Presentation: 近江八幡国民休暇村
  • Invited
• [Presentation] MutMap, MutMap+ & MutMap-Gap:次世代シーケンサーを用いた遺伝子単離技術
  • Author(s): 高木宏樹, 八重樫弘樹, 植村亜衣子, 宇津志博恵, 夏目俊, 阿部陽, 寺内良平
  • Organizer: 第１２４回日本育種学会講演会
  • Place of Presentation: 鹿児島大学
  • Invited
• [Presentation] 分子間相互作用によるイネ－いもち病菌の共進化
  • Author(s): 寺内良平, 神崎洋之, 齋藤宏昌, 藤崎恒喜, 高木宏樹
  • Organizer: 第１２４回日本育種学会講演会
  • Place of Presentation: 鹿児島大学
  • Invited
• [Presentation] 植物－病原菌相互作用の集団ゲノム解析
  • Author(s): 寺内良平
  • Organizer: 日本遺伝学会第８５回大会
  • Place of Presentation: 慶応大学
  • Invited
• [Presentation] Toward understanding evolution and function of Magnaporthe oryzae effectors AVR-Pia, AVR-Pii and AVR-Pik
  • Author(s): Terauchi,R., Saitoh,H., Kanzaki,H., Fujisaki,K., Takagi,H., Yoshida,K., Kamoun,S.
  • Organizer: 6th International Rice Blast Conference
  • Place of Presentation: Jeju, Korea
• [Presentation] Whole genome analysis of rice-Magnaporthe interactions
  • Author(s): Terauchi, R.
  • Organizer: 10th International Congress of Plant Pathology
  • Place of Presentation: Beijing, China
  • Invited
• [Remarks]
  • URL: http://www.ige.tohoku.ac.jp/prg/genetics/study_report/terauchi/