• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular basis of the intracellular life of bacteria within eukaryotic cells

Planned Research

Project Area"Matryoshka"-type evolution of eukaryotes
Project/Area Number 23117002
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionOsaka University

Principal Investigator

NAGAI Hiroki  大阪大学, 微生物病研究所, 准教授 (80222173)

Co-Investigator(Kenkyū-buntansha) YAMAGUCHI Hiroyuki  北海道大学, 保健科学研究院, 教授 (40221650)
MARUYAMA Fumito  京都大学, 医学系研究科, 准教授 (30423122)
Research Collaborator Xuan Thanh Bui  
Project Period (FY) 2011-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥97,760,000 (Direct Cost: ¥75,200,000、Indirect Cost: ¥22,560,000)
Fiscal Year 2015: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2014: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2013: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2012: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2011: ¥21,970,000 (Direct Cost: ¥16,900,000、Indirect Cost: ¥5,070,000)
Keywords細胞内共生 / 細胞内寄生 / 細菌 / アメーバ / レジオネラ / A群レンサ球菌
Outline of Final Research Achievements

Primary endosymbiosis, in which bacteria enter (pre-)eukaryotic cells and finally became mitochondria and chloroplasts, plays a critical role in eukaryogenesis. Even today, bacterial invasion into eukaryotic cells takes place every day at sites of infection. In this study, we focused on the relationship between various kinds of intracellular pathogenic/environmental bacteria and their eukaryotic host cells including free-living Acanthamoeba and human cells. We concluded several mechanistic principles which drive the first step of matryoshka-type evolution.

Report

(6 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (37 results)

All 2016 2015 2014 2013 2012 2011 Other

All Journal Article (12 results) (of which Peer Reviewed: 12 results,  Open Access: 4 results,  Acknowledgement Compliant: 4 results) Presentation (16 results) (of which Int'l Joint Research: 2 results,  Invited: 9 results) Remarks (9 results)

  • [Journal Article] The type IVB secretion system: an enigmatic chimera.2016

    • Author(s)
      Kubori T and *Nagai H.
    • Journal Title

      Curr Opin Microbiol.

      Volume: 29 Pages: 22-9

    • DOI

      10.1016/j.mib.2015.10.001

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Protozoal ciliate promotes bacterial autoinducer-2 accumulation in mixed culture with <i>Escherichia coli</i>2015

    • Author(s)
      Oguri S, Hanawa T, Matsuo J, Ishida K, Yamazaki T, Nakamura S, Okubo T, Fukumoto T, Akizawa K, Shimizu C, Kamiya S, Yamaguchi H
    • Journal Title

      The Journal of General and Applied Microbiology

      Volume: 61 Issue: 5 Pages: 203-210

    • DOI

      10.2323/jgam.61.203

    • NAID

      130005110102

    • ISSN
      0022-1260, 1349-8037
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Genetic profiles of Propionibacterium acnes and identification of a unique transposon with novel insertion sequences in sarcoid and non-sarcoid isolates.2015

    • Author(s)
      K. Minegishi, T. Watanabe, A. Furukawa, K. Uchida, Y. Suzuki, T. Akashi, F. Maruyama, I. Nakagawa, Y. Eishi
    • Journal Title

      Sci. Rep.

      Volume: 12 Issue: 1 Pages: 9832-9832

    • DOI

      10.1038/srep09832

    • NAID

      120005758210

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Draft Genome Sequence of High-Temperature-Adapted Protochlamydia sp. HS-T3, an Amoebal Endosymbiotic Bacterium Found in Acanthamoeba Isolated from a Hot Spring in Japan.2015

    • Author(s)
      Yamaguchi H, Matsuo J, Yamazaki T, Ishida K, Yagita K.
    • Journal Title

      Genome Announcements

      Volume: 5 Issue: 1

    • DOI

      10.1128/genomea.01507-14

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Amoebal endosymbiont Neochlamydia genome sequence illuminates the bacterial role in the defense of the host amoebae against Legionella pneumophila.2014

    • Author(s)
      Kasumi Ishida, Tsuyoshi Sekizuka, Kyoko Hayashida, Junji Matsuo, Fumihiko Takeuchi, Makoto Kuroda, Shinji Nakamura, Tomohiro Yamazaki, Mitsutaka Yoshida, Kaori Takahashi, HirokiNagai, Chihiro Sugimoto
    • Journal Title

      PLoS One

      Volume: 9 Issue: 4 Pages: e95166-e95166

    • DOI

      10.1371/journal.pone.0095166

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Native structure of a type IV secretion system core complex essential for Legionella pathogenesis2014

    • Author(s)
      Kubori T, Koike M, Bui XT, Higaki S, Aizawa S, *Nagai H
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 111 Issue: 32 Pages: 11804-11809

    • DOI

      10.1073/pnas.1404506111

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Modulation of the ubiquitination machinery by legionella2013

    • Author(s)
      Hubber A, Kubori T, *Nagai H
    • Journal Title

      Curr Top Microbiol Immunol

      Volume: 376 Pages: 227-247

    • DOI

      10.1007/82_2013_343

    • ISBN
      9783642405907, 9783642405914
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification and functional characterization of K(+) transporters encoded by Legionella pneumophila kup genes.2013

    • Author(s)
      Hori JI, Pereira MS, Roy CR, *Nagai H, Zamboni DS
    • Journal Title

      Cell Microbiol

      Volume: 15 Issue: 12 Pages: 2006-2019

    • DOI

      10.1111/cmi.12168

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Purification and characterization of legionella u-box-type e3 ubiquitin ligase2013

    • Author(s)
      Nagai H, Kubori T
    • Journal Title

      Methods Mol Biol

      Volume: 954 Pages: 347-354

    • DOI

      10.1007/978-1-62703-161-5_21

    • ISBN
      9781627031608, 9781627031615
    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Protochlamydia Induces Apoptosis of Human HEp-2 Cells through Mitochondrial Dysfunction Mediated by Chlamydial Protease-Like Activity Factor.2013

    • Author(s)
      Junji Matsuo, Shinji Nakamura, Atsushi Ito, Tomohiro Yamazaki, Kasumi Ishida. Yasuhiro Hayashi, Mitsutaka Yoshida, Kaori Takahashi, Tsuyoshi Sekizuka, Fumihiko Takeuchi, Makoto Kuroda, Hiroki Nagai, Kyoko Hayashida, Chihiro Sugimoto. Hiroyuki Yamaguchi
    • Journal Title

      PLoS One

      Volume: 8 Issue: 2 Pages: e56005-e56005

    • DOI

      10.1371/journal.pone.0056005

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dot/Icm type IVB secretion systems of Legionella and other gram-negative bacteria2011

    • Author(s)
      Hirok Nagai, Tomoko Kubori
    • Journal Title

      Front.Microbiol

      Volume: 2 Pages: 136-136

    • DOI

      10.3389/fmicb.2011.00136

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bacterial effector-involved temporal and spatial regulation by hijack of the host ubiquitin pathway2011

    • Author(s)
      Tomoko Kubori, Hiroki Nagai
    • Journal Title

      Front.Microbiol.

      Volume: 2

    • DOI

      10.3389/fmicb.2011.00145

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] 偶然による病原菌 レジオネラ2016

    • Author(s)
      永井 宏樹
    • Organizer
      共生・寄生生物学シンポジウム
    • Place of Presentation
      筑波大学
    • Year and Date
      2016-03-05
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Interdomain protein transfer as a critical mechanism for survival of intracellular bacteria.2015

    • Author(s)
      Hiroki Nagai
    • Organizer
      2015 International Meeting of "Matryoshka-type Evolution"
    • Place of Presentation
      筑波大学
    • Year and Date
      2015-09-30
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Type IV secretion system as a lethal weapon of bacterial pathogens2015

    • Author(s)
      Hiroki Nagai
    • Organizer
      The 10th International Symposium of the Institute Network "Towards the next generation research for cancer and Immunology"
    • Place of Presentation
      北海道大学
    • Year and Date
      2015-07-23
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] A 群レンサ球菌の宿主細胞内適応機構2015

    • Author(s)
      丸山史人
    • Organizer
      第88回 日本細菌学会総会
    • Place of Presentation
      長良川国際会議場(岐阜県岐阜市)
    • Year and Date
      2015-03-26 – 2015-03-28
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 原始的なクラミジアとアカントアメーバの共生様式から学ぶ2015

    • Author(s)
      山口博之
    • Organizer
      第88回 日本細菌学会総会
    • Place of Presentation
      長良川国際会議場(岐阜県岐阜市)
    • Year and Date
      2015-03-26 – 2015-03-28
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] レジオネラ病原性に必須なIV型分泌装置の構造生物学2015

    • Author(s)
      永井宏樹
    • Organizer
      第88回 日本細菌学会総会
    • Place of Presentation
      長良川国際会議場(岐阜県岐阜市)
    • Year and Date
      2015-03-26 – 2015-03-28
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Interdomain protein transfer as a critical mechanism for Matryoshka-type evolution2013

    • Author(s)
      永井宏樹
    • Organizer
      第2回マトリョーシカ型生物学研究会・公開国際シンポジウム
    • Place of Presentation
      ホテル京都ガーデンパレス
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Identification and characterization of host-specific effector proteins for the survival and growth of L. pneumophila within amoebae and mammalian cells2013

    • Author(s)
      Xuan Thanh Bui, Hiroki Nagai,
    • Organizer
      第2回マトリョーシカ型生物学研究会・公開国際シンポジウム
    • Place of Presentation
      ホテル京都ガーデンパレス
    • Related Report
      2013 Annual Research Report
  • [Presentation] Interdomain protein transport: a critical strategy employed by bacterial pathogens and symbionts2013

    • Author(s)
      Hiroki Nagai, Tomoko Kubori, Masafumi Koike, Saori Higaki and Shin-ichi Aizawa
    • Organizer
      第12回あわじしま感染症・免疫フォー ラム
    • Place of Presentation
      淡路夢舞台国際会議場
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Interdomain protein transport: a critical strategy employed by bacterial pathogens and symbionts、2012

    • Author(s)
      Nagai, H
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡(福岡県)
    • Year and Date
      2012-12-13
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 細菌の原虫・哺乳動物宿主に対する寄生・共生の分子基盤2012

    • Author(s)
      永井宏樹
    • Organizer
      第一回マトリョーシカ型生物学研究会
    • Place of Presentation
      国立感染症研究所、東京
    • Year and Date
      2012-07-22
    • Related Report
      2012 Annual Research Report
  • [Presentation] Structural insights highlight mosaic nature of the type IVB secretion system2012

    • Author(s)
      Hiroki Nagai, Tomoko Kubori, Noboru Nakano, Miki Kinoshita, Yumiko Uchida, Takuya Kuroda, Katsumi Imada
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎市
    • Year and Date
      2012-03-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] Bacterial effector-involved temporal and spatial regulation by hijack of the host ubiquitin pathway2012

    • Author(s)
      Tomoko Kubori, Hiroki Nagai
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎市(招待講演)
    • Year and Date
      2012-03-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] Plasma membrane phosphatidylinositol-4-phosphate (PI4P) regulates Legionella pneumophila effectors2012

    • Author(s)
      Andree Hubber, Kohei Arasaki, Fubito Nakatsu, Pietro De Camilli, Hiroki Nagai, Craig Roy
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎市
    • Year and Date
      2012-03-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] Structural insights highlight mosaic nature of the type IVB secretion system2011

    • Author(s)
      Hiroki Nagai
    • Organizer
      UNIA workshop "The Biology of Intracellular Bacterial Pathogens"
    • Place of Presentation
      Baeza, Spain
    • Year and Date
      2011-09-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] Type IVB secretion systems of Legionella and related bacteria. Deciphering core complex of the type IVB secretion system2011

    • Author(s)
      Hiroki Nagai
    • Organizer
      国際微生物学連盟2011会議(IUMS 2011)
    • Place of Presentation
      札幌市(招待講演)
    • Year and Date
      2011-09-07
    • Related Report
      2011 Annual Research Report
  • [Remarks] 領域webページ

    • URL

      http://www.matryoshka-evolution.jp/

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
  • [Remarks] 研究室webページ

    • URL

      http://nagailab.biken.osaka-u.ac.jp/

    • Related Report
      2015 Annual Research Report
  • [Remarks] 永井研究室webページ

    • URL

      http://nagailab.biken.osaka-u.ac.jp/

    • Related Report
      2014 Annual Research Report
  • [Remarks] 永井研究室Webページ

    • URL

      http://nagailab.biken.osaka-u.ac.jp/

    • Related Report
      2013 Annual Research Report
  • [Remarks] 領域Webページ

    • URL

      http://www.matryoshka-evolution.jp/

    • Related Report
      2013 Annual Research Report
  • [Remarks] 永井研究室Webページ

    • URL

      http://nagailab.biken.osaka-u.ac.jp/

    • Related Report
      2012 Annual Research Report
  • [Remarks] 領域Webページ

    • URL

      http://www.matryoshka-evolution.jp/

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://nagailab.biken.osaka-u.ac.jp

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.matryoshka-evolution.jp

    • Related Report
      2011 Annual Research Report

URL: 

Published: 2011-08-01   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi