|Budget Amount *help
¥91,260,000 (Direct Cost : ¥70,200,000、Indirect Cost : ¥21,060,000)
Fiscal Year 2015 : ¥17,160,000 (Direct Cost : ¥13,200,000、Indirect Cost : ¥3,960,000)
Fiscal Year 2014 : ¥17,160,000 (Direct Cost : ¥13,200,000、Indirect Cost : ¥3,960,000)
Fiscal Year 2013 : ¥18,200,000 (Direct Cost : ¥14,000,000、Indirect Cost : ¥4,200,000)
Fiscal Year 2012 : ¥18,070,000 (Direct Cost : ¥13,900,000、Indirect Cost : ¥4,170,000)
Fiscal Year 2011 : ¥20,670,000 (Direct Cost : ¥15,900,000、Indirect Cost : ¥4,770,000)
|Outline of Final Research Achievements
A green ciliate Paramecium bursaria harbors several hundred Chlorella cells in its cytoplasm. An electron microscopic approach with 3-D reconstruction showed that mitochondria in the host's cytoplasm provide a structural scaffold for symbiotic Chlorella to be anchored in the host's cytoplasm. Proteome and de novo transcriptome sequencing analyses were carried out to identify proteins in the peri-algal membrane fraction. As a distinctive aspect of the unicellular Matryoshka-type protists, P. bursaria was also found to accumulate soil-bound radioactive cesium.
Anaerobic protozoan Entamoeba species have mitosomes that are mitochondrion-derived organelles lacking DNA. Mitosomes isolated from E. histolytica were transplanted successfully into E. histolytica, E. nuttalli and E. invadens. Co-localization of mitosomal proteins from donor and recipient was observed in single mitosomes of some transplanted cells.