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Structure and Function of magnetotactic motility machinery

Planned Research

Project AreaHarmonized supramolecular machinery for motility and its diversity
Project/Area Number 24117007
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionKanazawa University

Principal Investigator

Fukumori Yoshihiro  金沢大学, その他部局等, 理事 (60135655)

Co-Investigator(Renkei-kenkyūsha) TAOKA Azuma  金沢大学, 理工研究域自然システム学系, 准教授 (20401888)
Project Period (FY) 2012-06-28 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥97,240,000 (Direct Cost: ¥74,800,000、Indirect Cost: ¥22,440,000)
Fiscal Year 2016: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2015: ¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2014: ¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2013: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2012: ¥32,630,000 (Direct Cost: ¥25,100,000、Indirect Cost: ¥7,530,000)
Keywords磁性細菌 / 細胞骨格 / オルガネラ / アクチン / べん毛 / マグネトソーム / 地磁気 / 磁気微粒子 / 走磁性 / ベン毛 / 原核細胞 / ヘム蛋白質 / イメージング / 走査プローブ顕微鏡 / 細菌 / 分子モーター
Outline of Final Research Achievements

Magnetotactic bacteria synthesize a nano-sized magnetic organelle termed the magnetosome, which they use to assist with their magnetic navigation in a specific bacterial motility called magnetotaxis. Magnetotactic bacteria are ubiquitous in aquatic environments in the world. However, the detailed molecular mechanisms of magneto-reception and magnetotaxis are still an enigma. Here, we focused on the function of MamK cytoskeleton, and analyzed molecular mechanisms of magnetotactic motility through the newly developed method for magnetotaxis quantification and the cutting-edge microscopic techniques, TIRF and high-speed AFM, for imaging dynamics of magnetosome and flagellum in living cells.

Report

(6 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (44 results)

All 2017 2016 2015 2014 2013 2012 Other

All Journal Article (13 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 12 results,  Open Access: 6 results,  Acknowledgement Compliant: 6 results) Presentation (29 results) (of which Int'l Joint Research: 3 results,  Invited: 7 results) Remarks (2 results)

  • [Journal Article] A protein-protein interaction in magnetosomes: TPR protein MamA interacts with an Mms6 protein2016

    • Author(s)
      H.V. Nguyen, E. Suzuki, Z. Oestreicher, H. Minamide, H. Endoh, Y. Fukumori, A. Taoka
    • Journal Title

      Biochemistry and Biophysics Reports

      Volume: 7 Pages: 39-44

    • DOI

      10.1016/j.bbrep.2016.05.010

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] A comparison of the surface nanostructure from two different types of gram-negative cells: Escherichia coli and Rhodobacter sphaeroides2015

    • Author(s)
      Oestreicher Z., Taoka A., Fukumori Y.
    • Journal Title

      Micron

      Volume: 72 Pages: 8-14

    • DOI

      10.1016/j.micron.2015.02.001

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Live-Cell Imaging of Bacterial Cells Using High-Speed Atomic Force Microscopy2015

    • Author(s)
      田岡 東、福森 義宏
    • Journal Title

      KAGAKU TO SEIBUTSU

      Volume: 53 Issue: 5 Pages: 293-298

    • DOI

      10.1271/kagakutoseibutsu.53.293

    • NAID

      130005146577

    • ISSN
      0453-073X, 1883-6852
    • Related Report
      2015 Annual Research Report
    • Acknowledgement Compliant
  • [Journal Article] The structure of a deoxygenated 400 kDa haemoglobin reveals ternary- and quaternary-structural changes of giant haemoglobins2014

    • Author(s)
      Numoto N., Nakagawa T., Ohara R., Hasegawa T., Kita A., Yoshida T., Maruyama T., Imai K., Fukumori Y., Miki K.
    • Journal Title

      Acta Cryst.

      Volume: D70 Pages: 1823-1831

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A magnetosome-associated cytochrome MamP is critical for magnetite crystal growth during the exponential growth phase2014

    • Author(s)
      Taoka A., Eguchi Y., Mise S., Oestreicher Z., Uno, F., Fukumori Y.
    • Journal Title

      FEMS Microbiol. Lett.

      Volume: 358 Pages: 21-29

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Characterization of uncultured giant rod-shaped magnetotactic Gammaproteobacteria from a fresh water pond in Kanazawa, Japan2014

    • Author(s)
      Taoka A., Kondo J., Oestreicher Z., Fukumori Y.
    • Journal Title

      Microbiology-SGM.

      Volume: 160 Pages: 2226-2234

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 磁性細菌オルガネラ「マグネトソーム」の構造機能相関の解2014

    • Author(s)
      福森 義宏, 田岡 東
    • Journal Title

      生物物理

      Volume: 54 Pages: 11-14

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] DnaJ-promoted binding of DnaK to multiple sites on σ32 in the presence of ATP2014

    • Author(s)
      3. A.Noguchi, A.Ikeda, M.Mezaki, Y.Fukumori, and M.Kanemori
    • Journal Title

      J.Bact.

      Volume: 196 Pages: 1694-1703

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of magnetotactic behavior by swimming assay2013

    • Author(s)
      1. S. Sakaguchi, A. Taoka, and Y. Fukumori
    • Journal Title

      Biosci. Biotech. and Biochem.

      Volume: 77 Pages: 940-947

    • NAID

      10031177578

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Purification and characterization of coacervate-forming cuticular proteins from Papilio xuthus Pupae2013

    • Author(s)
      2. M. Yamanaka, Y. Ishizaki, T. Nakagawa, A. Taoka, and Y. Fukumori
    • Journal Title

      Zoological Sci.

      Volume: 30 Pages: 534-542

    • NAID

      40019687033

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of Magnetotactic Behavior By Swimming Assay.2013

    • Author(s)
      Shoutaro Sakaguchi
    • Journal Title

      Bioscience,Biotechnology,and Biochemistry

      Volume: in press

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Single molecule imaging on living bacterial cell surface by high-speed AFM2012

    • Author(s)
      Hayato Yamashita
    • Journal Title

      Journal of Molecular Biology

      Volume: 422 Issue: 2 Pages: 300-309

    • DOI

      10.1016/j.jmb.2012.05.018

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synergistic binding of DnaJ and DnaK chaperone to the heat shock transcription factor σ32 assures its characteristic high metabolic instability: Implications for2012

    • Author(s)
      Hirotaka Suzuki
    • Journal Title

      The Journal of biological Chemistry

      Volume: 287 Issue: 23 Pages: 19275-19283

    • DOI

      10.1074/jbc.m111.331470

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] 磁性細菌Magnetospirillum magneticum AMB-1のべん毛回転運動の可視化2017

    • Author(s)
      川村 想、高岡 裕太、田岡 東、中根 大介、西坂 崇之、福森 義宏
    • Organizer
      2017年 生体運動合同班会議
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2017-01-07
    • Related Report
      2016 Annual Research Report
  • [Presentation] The actin-like cytoskeletal protein MamK plays a role in positioning of magnetic organelles for bacterial magnetotactic motility2016

    • Author(s)
      田岡 東、福森 義宏
    • Organizer
      第54回日本生物物理学会年会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2016-11-27
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] A new look at the nanostructure of the outer surface of living Proteobacteria using the high-speed atomic force microscope2016

    • Author(s)
      Taoka Azuma, Zachery Oestreicher, Hayato Yamashita, Takayuki Uchihashi, Toshio Ando, Yoshihiro Fukumori
    • Organizer
      4th Kanazawa Bio-AFM Workshop
    • Place of Presentation
      KKRホテル金沢
    • Year and Date
      2016-10-04
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] アクチン様細胞骨格蛋白質MamKとそのATPase活性のマグネトソーム細胞内配置における役割2016

    • Author(s)
      清河文子, 田岡東, 福森義宏
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2016-09-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] The actin-like cytoskeletal protein MamK and its ATPase activity play a role in the positioning of magnetosomes2016

    • Author(s)
      A. Taoka, A. Kiyokawa, C. Uesugi, Z. Oestreicher, and Y. Fukumori
    • Organizer
      Magnetotactic Bacteria 5th International Meeting
    • Place of Presentation
      Marseille (France)
    • Year and Date
      2016-09-12
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 磁性細菌の遊泳運動—アクチン様細胞骨格の役割2016

    • Author(s)
      田岡 東、福森 義宏
    • Organizer
      第89回日本細菌学会総会シンポジウム「細菌の運動」
    • Place of Presentation
      大阪国際交流センター
    • Year and Date
      2016-03-23
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 磁性細菌ゲノムに保存されたアクチン様細胞骨格蛋白質MamKによるマグネトソーム配置調節2016

    • Author(s)
      田岡 東、清河文子、上杉知佳、福森義宏
    • Organizer
      第10回日本ゲノム微生物学会年会
    • Place of Presentation
      東京工業大学大岡山キャンパス
    • Year and Date
      2016-03-04
    • Related Report
      2015 Annual Research Report
  • [Presentation] 細菌のオルガネラ「マグネトソーム」の細胞内配置調節に関わるアクチン様細胞骨格蛋白質2016

    • Author(s)
      田岡 東、清河文子、福森義宏
    • Organizer
      生体運動合同班会議
    • Place of Presentation
      キャンパスプラザ京都
    • Year and Date
      2016-01-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] 磁性細菌のマグネタイトの生合成と細胞質膜局在c 型シトクロムMamP の関連性の研究2015

    • Author(s)
      江口友佳子、田岡 東、福森義宏
    • Organizer
      第38回日本分子生物学会、第88回日本生化学会合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-12-02
    • Related Report
      2015 Annual Research Report
  • [Presentation] 生細胞蛍光イメージングを用いた磁性細菌のアクチン様細胞骨格MamKの機能解析2015

    • Author(s)
      清河 文子、田岡 東、福森 義宏
    • Organizer
      第38回日本分子生物学会、第88回日本生化学会合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] Protein interactions in bacterial organelle magnetosomes: TPR protein MamA interacting with N-terminal region of Mms62015

    • Author(s)
      Nguyen Viet Hoang, Azuma Taoka, Emi Suzuki, Yoshihiro Fukumori
    • Organizer
      第38回日本分子生物学会、第88回日本生化学会合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] 磁性細菌に特異的な細胞骨格蛋白質MamKによるマグネトソーム細胞内配置調節2015

    • Author(s)
      田岡 東、清河 文子、上杉 知佳、福森 義宏
    • Organizer
      2015 年度日本農芸化学会中部・関西支部合同大会
    • Place of Presentation
      富山県立大学
    • Year and Date
      2015-09-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] The high-speed atomic force microscope as a tool for examining living Escherichia coli cells2015

    • Author(s)
      1.Zachery Oestreicher, Azuma Taoka, and Yoshihiro Fukumori
    • Organizer
      第12 回21 世紀大腸菌研究会
    • Place of Presentation
      琵琶湖グランドホテル・京近江
    • Year and Date
      2015-06-05
    • Related Report
      2015 Annual Research Report
  • [Presentation] 生細胞蛍光イメージングを用いた磁性細菌のアクチン様細胞骨格MamKの機能解析2015

    • Author(s)
      田岡 東、清河 文子、上杉 知佳、福森 義宏
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      岡山大学津島キャンパス
    • Year and Date
      2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] マグネタイトの生合成における磁性細菌特有のc型シトクロムMamPの役割2015

    • Author(s)
      江口 友佳子、見世 慎吾、田岡 東、福森 義宏
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      岡山大学津島キャンパス
    • Year and Date
      2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] 脱窒と酸素呼吸に関する進化的考察2014

    • Author(s)
      福森義宏
    • Organizer
      第37 回分子生物学会年会シンポジウム「生命の起源・進化・本質」
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-25
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 高速原子間力顕微鏡(高速AFM)で「みる」バクテリアの細胞表層2014

    • Author(s)
      田岡 東、山下隼人、Zachery Oestreicher、福森義宏
    • Organizer
      環境微生物系学会合同大会2014 シンポジウム「微生物を見る」
    • Place of Presentation
      浜松アクトシティコングレスセンター,静岡
    • Year and Date
      2014-10-22
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Fluorescence live-cell imaging for visualizing the subcellular dynamics of magnetosomes2014

    • Author(s)
      A. Taoka, C. Uesugi, Z. Oestreicher, K. Morii, A. Kiyokawa, Y. Fukumori,
    • Organizer
      4th International Meeting on Magnetotactic Bacteria
    • Place of Presentation
      Rio de Janeiro, Brazil,
    • Year and Date
      2014-09-15
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 磁性細菌の磁気オルガネラに関与するアクチン様細胞骨格の機能解析2013

    • Author(s)
      坂口祥太朗 他
    • Organizer
      2013年 生体運動研究合同班会議
    • Place of Presentation
      広島大学東広島キャンパス(広島県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Functional Analysis of Actin-Like Cytoskeletal Protein MamK Associated with Prokaryotic Organelle Magnetosomes2013

    • Author(s)
      Azuma Taoka 他
    • Organizer
      The 23rd CDB Meeting : Building multicellular systems form cellular cross-talk
    • Place of Presentation
      RIKEN center for developmental biology (兵庫県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 生きた細菌細胞表層のナノオーダー構造解析2013

    • Author(s)
      田岡 東 他
    • Organizer
      第86回日本細菌学会総会
    • Place of Presentation
      幕張メッセ(千葉県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 生物磁気微粒子の生合成に関わるヘム蛋白質MamPの機能解析2012

    • Author(s)
      江口 友佳子 他
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡(福岡県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 高速AFMによる生きた原核細胞表面における分子動態イメージング2012

    • Author(s)
      田岡 東 他
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡(福岡県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 磁性細菌の磁気オルガネラ形成に関わる細胞骨格MamKの重合特性2012

    • Author(s)
      島尻翔 他
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡(福岡県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] マグネトソーム局在タンパク質の機能解析のための走磁性測定方法の開発2012

    • Author(s)
      坂口 祥太朗 他
    • Organizer
      第49回日本細菌学会中部支部総会
    • Place of Presentation
      金沢大学医学部十全講堂(石川県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 高速原子間力顕微鏡による細菌外膜構造の動態イメージング2012

    • Author(s)
      福森 義宏
    • Organizer
      第49回日本細菌学会中部支部総会
    • Place of Presentation
      金沢大学医学部十全講堂(石川県)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 原核細胞オルガネラ[マグネトソーム」に局在する新奇ヘム蛋白質MamPの役割

    • Author(s)
      江口友佳子ほか3名
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      横浜
    • Related Report
      2013 Annual Research Report
  • [Presentation] マグネトソームアイランドにコードされるFysZ様蛋白質のキャラクタリゼーション

    • Author(s)
      森健太ほか3名
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      横浜
    • Related Report
      2013 Annual Research Report
  • [Presentation] 脱窒細菌Paracoccus denitrificansの一酸化窒素還元酵素の精製と阻害因子の探索

    • Author(s)
      鶴ヶ崎慎太郎ほか2名
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      横浜
    • Related Report
      2013 Annual Research Report
  • [Remarks] 動物・微生物生理学研究室研究業績

    • URL

      http://pronet.s.kanazawa-u.ac.jp/j/research.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 研究業績

    • URL

      http://pronet.s.kanazawa-u.ac.jp/j/research.html

    • Related Report
      2014 Annual Research Report

URL: 

Published: 2012-11-27   Modified: 2019-07-29  

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