|Budget Amount *help
¥97,890,000 (Direct Cost: ¥75,300,000、Indirect Cost: ¥22,590,000)
Fiscal Year 2016: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2015: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2014: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2013: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2012: ¥22,880,000 (Direct Cost: ¥17,600,000、Indirect Cost: ¥5,280,000)
|Outline of Final Research Achievements
Transcription cycle consists of various protein factors, which interact in different ways depending on their stage in the cycle. Single-molecule approaches provide quantitative and high-resolution analysis, through capturing heterogeneous behavior of the protein factors.
By installing a single-molecule fluorescence microscope based on our original HILO illumination method, we analyzed the interaction of transcription factors and molecular dynamics of chromatin remodeling complex. Super-resolution imaging with HILO microscopy realized high-resolution analysis in the spatial distribution of transcription-related proteins. We further developed an image-analyzing method using single-molecule tracking approach. Combining this technique with three-color simultaneous single-molecule imaging, we quantified the dynamics and kinetics of molecules. This approach facilitates the spatiotemporal quantification of the dynamics and kinetics of interactions of protein factors in transcription cycle.