Time-resolved Observation and Elucidation of Functionally-important Molecular Flexibility

• Project Area: Science on Function of Soft Molecular Systems by Cooperation of Theory and Experiment
• Project/Area Number: 25104006
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Osaka University
• Principal Investigator: Mizutani Yasuhisa 大阪大学, 理学研究科, 教授 (60270469)
• Co-Investigator(Kenkyū-buntansha): 水野 操 大阪大学, 理学研究科, 助教 (10464257) ; 石川 春人 大阪大学, 理学研究科, 講師 (40551338)
• Project Period (FY): 2013-06-28 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥111,280,000 (Direct Cost: ¥85,600,000、Indirect Cost: ¥25,680,000); Fiscal Year 2017: ¥21,190,000 (Direct Cost: ¥16,300,000、Indirect Cost: ¥4,890,000); Fiscal Year 2016: ¥21,190,000 (Direct Cost: ¥16,300,000、Indirect Cost: ¥4,890,000); Fiscal Year 2015: ¥20,280,000 (Direct Cost: ¥15,600,000、Indirect Cost: ¥4,680,000); Fiscal Year 2014: ¥21,190,000 (Direct Cost: ¥16,300,000、Indirect Cost: ¥4,890,000); Fiscal Year 2013: ¥27,430,000 (Direct Cost: ¥21,100,000、Indirect Cost: ¥6,330,000)
• Keywords: ラマン分光学 / アロステリー / 時間分解分光学 / 時間分解分光法

Outline of Final Research Achievements

Proteins can exhibit high functionality which artificial molecules cannot do. Thus, understanding of protein helps us not only to understand life phenomena but also to create highly functional molecules. Because coupling of functional units in structural changes is indispensable for proteins to function, dynamic structure as well as static one of proteins is needed to be elucidated. We revealed structural changes important for allostery and energy flow driving functionally-important motions mainly by using time-resolved resonance Raman spectroscopy.

Research Products

• [Journal Article] Tertiary Dynamics of Human Adult Hemoglobin Fixed in R and T Quaternary Structures (2018)
  • Author(s): Shanyan Chang, Misao Mizuno, Haruto Ishikawa and Yasuhisa Mizutani
  • Journal Title: Phys. Chem. Chem. Phys. Volume: 20 Issue: 5 Pages: 3363-3372
  • DOI: 10.1039/c7cp06287g
  • Peer Reviewed
• [Journal Article] Structural Evolution of a Retinal Chromophore in the Photocycle of Halorhodopsin from Natronobacterium pharaonis (2018)
  • Author(s): Misao Mizuno, Ayumi Nakajima, Hideki Kandori, and Yasuhisa Mizutani
  • Journal Title: J. Phys. Chem. A Volume: 122 Issue: 9 Pages: 2411-2423
  • DOI: 10.1021/acs.jpca.7b12332
  • Peer Reviewed
• [Journal Article] Opn5L1 is a retinal receptor that behaves as a reverse and self-regenerating photoreceptor (2018)
  • Author(s): Sato Keita、Yamashita Takahiro、Ohuchi Hideyo、Takeuchi Atsuko、Gotoh Hitoshi、Ono Katsuhiko、Mizuno Misao、Mizutani Yasuhisa、Tomonari Sayuri、Sakai Kazumi、Imamoto Yasushi、Wada Akimori、Shichida Yoshinori
  • Journal Title: Nature Communications Volume: 9 Issue: 1 Pages: 1255-1255
  • DOI: 10.1038/s41467-018-03603-3
  • NAID: 120006535079
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Time-Resolved Resonance Raman Spectroscopy and Application to Studies on Ultrafast Protein Dynamics (2017)
  • Author(s): Mizutani Yasuhisa
  • Journal Title: Bulletin of the Chemical Society of Japan Volume: 90 Issue: 12 Pages: 1344-1371
  • DOI: 10.1246/bcsj.20170218
  • NAID: 130006250360
  • ISSN: 0009-2673, 1348-0634
  • Peer Reviewed
• [Journal Article] Chromophore Structure of Photochromic Fluorescent Protein Dronpa: Acid-base Equilibrium of Two cis Configurations (2016)
  • Author(s): Asuka Higashino, Misao Mizuno, and Yasuhisa Mizutani
  • Journal Title: The Journal of Physical Chemistry B Volume: 120 Issue: 13 Pages: 3353-3353
  • DOI: 10.1021/acs.jpcb.6b01752
  • Peer Reviewed
• [Journal Article] Importance of Atomic Contacts in Vibrational Energy Flow in Proteins (2016)
  • Author(s): Masato Kondoh, Misao Mizuno, and Yasuhisa Mizutani
  • Journal Title: J. Phys. Chem. Lett. Volume: 7 Issue: 11 Pages: 1950-1954
  • DOI: 10.1021/acs.jpclett.6b00785
  • Peer Reviewed
• [Journal Article] Regulatory Implications of Structural Changes in Tyr201 of the Oxygen Sensor Protein FixL (2016)
  • Author(s): Takeo Yamawaki, Haruto Ishikawa, Misao Mizuno, Hiro Nakamura, Yoshitsugu Shiro, and Yasuhisa Mizutani
  • Journal Title: Biochemistry Volume: 55 Issue: 29 Pages: 4027-4035
  • DOI: 10.1021/acs.biochem.6b00405
  • Peer Reviewed
• [Journal Article] A Study of the Dynamics of the Heme Pocket and C-helix in CooA Upon CO Dissociation Using Time-resolved Visible and UV Resonance Raman Spectroscopy (2016)
  • Author(s): Akihiro Otomo, Haruto Ishikawa, Misao Mizuno, Tetsunari Kimura, Minoru Kubo, Yoshitsugu Shiro, Shigetoshi Aono, and Yasuhisa Mizutani
  • Journal Title: J. Phys. Chem. B Volume: 120 Issue: 32 Pages: 7836-7843
  • DOI: 10.1021/acs.jpcb.6b05634
  • Peer Reviewed
• [Journal Article] Effect of the N-terminal residues on the quaternary dynamics of human adult hemoglobin (2016)
  • Author(s): Shanyan Chang, Misao Mizuno, Haruto Ishikawa, and Yasuhisa Mizutani
  • Journal Title: Chemical Physics Volume: 469-470 Pages: 31-37
  • DOI: 10.1016/j.chemphys.2016.02.009
  • Peer Reviewed
• [Journal Article] Carbon monoxide binding properties of domain-swapped dimeric myoglobin (2015)
  • Author(s): Satoshi Nagao, Haruto Ishikawa, Takuya Yamada, Yasuhisa Mizutani, Shun Hirota
  • Journal Title: Journal of biological inorganic chemistry Volume: 20 Issue: 3 Pages: 523-530
  • DOI: 10.1007/s00775-014-1236-0
  • NAID: 120005716870
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Protein response to chromophore isomerization in microbial rhodopsins revealed by picosecond time-resolved ultraviolet resonance Raman spectroscopy: a review (2015)
  • Author(s): Misao Mizuno, and Yasuhisa Mizutani
  • Journal Title: ACS Symposium Series eBooks, Recent Progress in Surface and Colloids Chemistry with Biological Applications Volume: 1215 Pages: 329-353
  • DOI: 10.1021/bk-2015-1215.ch016
  • ISBN: 9780841231207, 9780841231191
  • Peer Reviewed
• [Journal Article] Real-time Observation of the Perutz Mechanism in Hemoglobin Quaternary Revealed by Time-resolved Resonance Raman Spectroscopy (2015)
  • Author(s): 水谷泰久
  • Journal Title: Seibutsu Butsuri Volume: 55 Issue: 2 Pages: 095-097
  • DOI: 10.2142/biophys.55.095
  • NAID: 130005061234
  • ISSN: 0582-4052, 1347-4219
  • Peer Reviewed / Open Access
• [Journal Article] Progress of Laser Technology and Observation of Ultrafast Protein Dynamics (2015)
  • Author(s): 水野 操，水谷 泰久
  • Journal Title: Seibutsu Butsuri Volume: 55 Issue: 6 Pages: 322-328
  • DOI: 10.2142/biophys.55.322
  • NAID: 130005110034
  • ISSN: 0582-4052, 1347-4219
  • Peer Reviewed / Open Access
• [Journal Article] Critical Histidine Residues Regulate Hemozoin Formation Activity of the Heme Detoxification Protein in Plasmodium falciparum (2014)
  • Author(s): Keisuke Nakatani, Haruto Ishikawa, Shigetoshi Aono, Yasuhisa Mizutani
  • Journal Title: Sci. Rep. Volume: 4 Issue: 1 Pages: 6137-6137
  • DOI: 10.1038/srep06137
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Observing Vibrational Energy Flow in a Protein with the Spatial Resolution of a Single Amino Acid Residue (2014)
  • Author(s): Naoki Fujii, Misao Mizuno, Haruto Ishikawa, and Yasuhisa Mizutani
  • Journal Title: J. Phys. Chem. Lett. Volume: 5 Issue: 18 Pages: 3269-3273
  • DOI: 10.1021/jz501882h
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Intersubunit communication via changes in hemoglobin quaternary structures revealed by time-resolved resonance Raman spectroscopy: Direct observation of the Perutz mechanism (2013)
  • Author(s): Kenta Yamada, Haruto Ishikawa, Misao Mizuno, Naoya Shibayama, and Yasuhisa Mizutani
  • Journal Title: J. Phys. Chem. B Volume: 117 Issue: 41 Pages: 12461-12468
  • DOI: 10.1021/jp407735t
  • Peer Reviewed
• [Journal Article] Ultraviolet resonance Raman observations of the structural dynamics of rhizobial oxygen sensor FixL on ligand recognition (2013)
  • Author(s): Shinji Yano, Haruto Ishikawa, Misao Mizuno, Hiro Nakamura, Yoshitsugu Shiro, Yasuhisa Mizutani
  • Journal Title: Journal of Physical Chemistry B Volume: 117 Issue: 49 Pages: 15786-15791
  • DOI: 10.1021/jp406709e
  • Peer Reviewed
• [Journal Article] タンパク質分子における“熱”エネルギーの散逸過程 (2013)
  • Author(s): 水野 操, 水谷 泰久
  • Journal Title: 熱測定 Volume: 40 Pages: 139-145
  • NAID: 40019842757
  • Peer Reviewed
• [Presentation] ナトリウムイオン結合に伴う光駆動型ナトリウムイオンポンプKR2の構造変化観測 (2018)
  • Author(s): 大友 章裕、水野 操、井上 圭一、神取 秀樹、水谷 泰久
  • Organizer: 日本化学会第97春季年会
• [Presentation] 好熱菌由来光駆動プロトンポンプの発色団構造および多量体安定性に膜環境が与える効果 (2018)
  • Author(s): 塩谷智巳、水野操、塚本卓、須藤雄気、水谷泰久
  • Organizer: 日本化学会第97春季年会
• [Presentation] 原子間接触を介したタンパク質内振動エネルギー移動経路 (2018)
  • Author(s): 山下聡、水野操、水谷泰久
  • Organizer: 日本化学会第97春季年会
• [Presentation] 時間分解共鳴ラマン分光法を研ぐ (2018)
  • Author(s): 水谷泰久
  • Organizer: レーザー学会学術講演会第38回年次大会
  • Invited
• [Presentation] Importance of Atomic Contacts in Vibrational Energy Flow in Protein (2017)
  • Author(s): Masato Kondoh, Satoshi Yamashita, Misao Mizuno, and Yasuhisa Mizutani
  • Organizer: Eighteenth International Conference on Time-Resolved Vibrational Spectroscopy
  • Int'l Joint Research
• [Presentation] Measurements of Temperature Dependence on Anti-Stokes Raman Spectra for Quantitative Analysis of Energy Transfer in Heme Proteins (2017)
  • Author(s): Satoshi Yamashita, Misao Mizuno, and Yasuhisa
  • Organizer: The 6th Asian Spectroscopy Conference
  • Int'l Joint Research
• [Presentation] Subunit-subunit interaction in a light-driven proton pump from a thermophilic bacterium: Effects on hydrogen bond of the chromophore (2017)
  • Author(s): Tomomi Shionoya, Misao Mizuno, Takashi Tsukamoto, Yuki Sudo, and Yasuhisa Mizutani
  • Organizer: The 6th Asian Spectroscopy Conference
  • Int'l Joint Research
• [Presentation] ナトリウムイオンポンプの発色団構造と光駆動カチオン輸送機構 (2017)
  • Author(s): 水野 操、神取 秀樹、水谷 泰久
  • Organizer: 第11回分子科学討論会
• [Presentation] 光駆動型ナトリウムイオン輸送タンパク質KR2のイオンに依存した立体構造の違い (2017)
  • Author(s): 大友 章裕、水野 操、井上 圭一、神取 秀樹、水谷 泰久
  • Organizer: 第11回分子科学討論会
• [Presentation] 好熱菌由来光駆動プロトンポンプTRのタンパク質環境が発色団構造に及ぼす影響 (2017)
  • Author(s): 塩谷 智巳、水野 操、塚本 卓、須藤 雄気、水谷 泰久
  • Organizer: 第11回分子科学討論会
• [Presentation] タンパク質における振動エネルギーの移動経路 (2017)
  • Author(s): 山下 聡、水野 操、水谷 泰久
  • Organizer: 第11回分子科学討論会
• [Presentation] Determining factors for ion pumping mechanism in microbial rhodopsins (2017)
  • Author(s): Misao Mizuno
  • Organizer: Eighteenth International Conference on Time-Resolved Vibrational Spectroscopy
  • Int'l Joint Research / Invited
• [Presentation] Importance of atomic contacts to propagations in protein: functional compactness (2017)
  • Author(s): Yasuhisa Mizutani
  • Organizer: Telluride Science Research Center Workshop on Protein Dynamics
  • Int'l Joint Research / Invited
• [Presentation] Time-resolved resonance Raman study on roles of hydrogen bonds in light-driven ion-pumping proteins (2017)
  • Author(s): Yasuhisa Mizutani
  • Organizer: The 6th Asian Spectroscopy Conference
  • Int'l Joint Research / Invited
• [Presentation] 好熱菌由来光駆動プロトンポンプのサブユニット間相互作用：発色団水素結合に及ぼす影響 (2017)
  • Author(s): 塩谷智巳、水野操、塚本卓、須藤雄気、水谷泰久
  • Organizer: 日本化学会第96春季年会
• [Presentation] Spectral features of heme with higher symmetry in myoglobin (2017)
  • Author(s): KAWARABATA, Momoko; MIZUNO, Misao; MIZUTANI, Yasuhisa
  • Organizer: 日本化学会第96春季年会
• [Presentation] タンパク質内エネルギー移動の定量化に向けたアンチストークスラマンスペクトルの温度依存性の観測 (2017)
  • Author(s): 山下聡、水野操、水谷泰久
  • Organizer: 日本化学会第96春季年会
• [Presentation] Direct Observation of Vibrational Energy Flow in Proteins by Time-resolved Resonance Raman Spectroscopy (2017)
  • Author(s): Yasuhisa Mizutani
  • Organizer: Present and Future of Ultrafast Spectroscopy
  • Int'l Joint Research / Invited
• [Presentation] グロイオバクターロドプシンのシッフ塩基とプロトンドナー間にみられる長距離相互作用 (2016)
  • Author(s): 及川 健太郎、水野 操、神取秀樹、水谷 泰久
  • Organizer: 第43回生体分子科学討論会
• [Presentation] ハロロドプシン光反応中間体のレチナール発色団に対する水分子の役割 (2016)
  • Author(s): 水野 操、神取 秀樹、水谷 泰久
  • Organizer: 第10回分子科学討論会
• [Presentation] 酸素センサータンパク質FixLのアロステリックダイナミクス (2016)
  • Author(s): 山脇 竹生、水野 操、石川 春人、中村 寛夫、城 宜嗣、水谷 泰久
  • Organizer: 第10回分子科学討論会
• [Presentation] 光駆動塩化物イオンポンプとそのプロトンポンプへ機能転換した変異体の光反応における発色団構造の違い (2016)
  • Author(s): 久保田 真司、水野 操、神取 秀樹、水谷 泰久
  • Organizer: 第10回分子科学討論会
• [Presentation] Structure of retinal chromophore of the photointermediates in sodium ion pump rhodopsin (2016)
  • Author(s): 西村 尚、水野 操、神取 秀樹、水谷 泰久
  • Organizer: 日本生物物理学会第54回年会
• [Presentation] Structural evolution of the retinal chromophore in the photocycle of microbial rhodopsins (2016)
  • Author(s): Misao Mizuno
  • Organizer: 4th Awaji International Workshop on “Electron Spin Science & Technology: Biological and Materials Science Oriented Applications”
  • Int'l Joint Research / Invited
• [Presentation] Watching vibrational energy flow in proteins (2016)
  • Author(s): Yasuhisa Mizutani
  • Organizer: International Symposium: "Recent Progress in Molecular Spectroscopy and Dynamics"
  • Int'l Joint Research / Invited
• [Presentation] 時間分解共鳴ラマン分光法の開拓とタンパク質ダイナミクス研究 (2016)
  • Author(s): 水谷泰久
  • Organizer: 九重分子科学セミナー2016
  • Invited
• [Presentation] 紫外共鳴ラマン分光法とPhos-tag電気泳動法により明らかになった酸素ガスセンサーFixLの作動機構 (2016)
  • Author(s): 水谷泰久
  • Organizer: 第89回日本生化学会大会
  • Invited
• [Presentation] Importance of atomic contacts in vibrational energy flow in proteins (2016)
  • Author(s): Yasuhisa Mizutani
  • Organizer: 6th International Conference on Perspectives in Vibrational Spectroscopy
  • Int'l Joint Research / Invited
• [Presentation] タンパク質を基盤とした酸素及びヘム濃度プローブ分子の開発 (2016)
  • Author(s): 石川春人
  • Organizer: 第54回日本生物物理学会年会
  • Invited
• [Presentation] Long-range interaction between the chromophore and the proton donor of Gloeobacter rhodopsin (2016)
  • Author(s): Kentaro Oikawa, Misao Mizuno, Hideki Kandori, and Yasuhisa Mizutani
  • Organizer: 17th International Conference on Retinal Proteins
  • Int'l Joint Research
• [Presentation] Ion pumping and hydrogen bond of the protonated Schiff base in microbial rhodopsins (2016)
  • Author(s): Yasuhisa Mizutani, Misao Mizuno, Shinji Kubota, Kentaro Oikawa, Nao Nishimura, Ayumi Nakajima, and Hideki Kandori
  • Organizer: 17th International Conference on Retinal Proteins
  • Int'l Joint Research
• [Presentation] emporal Raman mapping of vibrational energy flow in hemeprotein (2015)
  • Author(s): Yasuhisa Mizutani
  • Organizer: Pacifichem 2015
  • Place of Presentation: Honolulu, Hawaii, USA
  • Year and Date: 2015-12-15
  • Int'l Joint Research / Invited
• [Presentation] Roles of Structural Changes around Tyr201 in Kinase Regulation of Oxygen Sensor Protein FixL (2015)
  • Author(s): Takeo Yamawaki, Haruto Ishikawa, Misao Mizuno, Hiro Nakamura, Yoshitsugu Shiro, Yasuhisa Mizutani
  • Organizer: Pacifichem 2015
  • Place of Presentation: Honolulu, Hawaii, USA
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] ナトリウムイオンポンプ型ロドプシンの発色団中間体構造：共鳴ラマン分光法による観測 (2015)
  • Author(s): 西村尚、水野操
  • Organizer: 新学術領域「柔らかな分子系」第 13 回 ワークショップ 「光駆動ナトリウムポンプからタンパク質の柔らかさと機能のつながりを考える」
  • Place of Presentation: 犬山
  • Year and Date: 2015-10-28
  • Invited
• [Presentation] Watching energy flow in proteins (2015)
  • Author(s): Yasuhisa Mizutani
  • Organizer: The Fifth Asian Spectroscopy Conference
  • Place of Presentation: Sydney, Australia
  • Year and Date: 2015-09-29
  • Int'l Joint Research / Invited
• [Presentation] Structural evolution of the retinal chromophore in the photocycle of microbial ion pumps (2015)
  • Author(s): Misao Mizuno
  • Organizer: The Fifth Asian Spectroscopy Conference
  • Place of Presentation: Sydney, Australia
  • Year and Date: 2015-09-29
  • Int'l Joint Research / Invited
• [Presentation] Long-range coupling between the chromophore and the proton donor in the K intermediate of Gloeobacter rhodopsin (2015)
  • Author(s): Kentaro Oikawa, Misao Mizuno, Hideki Kandori, Yasuhisa Mizutani
  • Organizer: 11th Australasian Conference on Vibrational Spectroscopy and the 5th Asian Spectroscopy Conference
  • Place of Presentation: Sydney, Australia
  • Year and Date: 2015-09-29
  • Int'l Joint Research
• [Presentation] Chromophore structures in the early intermediates of light-driven chloride ion pumps (2015)
  • Author(s): Shinji Kubota, Misao Mizuno, Hideki Kandori, Yasuhisa Mizutani
  • Organizer: 11th Australasian Conference on Vibrational Spectroscopy and the 5th Asian Spectroscopy Conference
  • Place of Presentation: Sydney, Australia
  • Year and Date: 2015-09-29
  • Int'l Joint Research
• [Presentation] グロイオバクターロドプシンのプロトンポンプ機能を促進する発色団構造変化 (2015)
  • Author(s): 中嶋 亜侑美、水野 操、神取 秀樹、水谷 泰久
  • Organizer: 第9回分子科学討論会
  • Place of Presentation: 東京
  • Year and Date: 2015-09-16
• [Presentation] 水素結合強度変化によるFixLのリン酸化活性制御機構 (2015)
  • Author(s): 山脇 竹生、石川 春人、水野 操、中村 寛夫、城 宜嗣、水谷 泰久
  • Organizer: 第9回分子科学討論会
  • Place of Presentation: 東京
  • Year and Date: 2015-09-16
• [Presentation] グロイオバクターロドプシンK中間体における発色団とプロトンドナーとの長距離カップリング (2015)
  • Author(s): 及川 健太郎、水野 操、神取 秀樹、水谷 泰久
  • Organizer: 第9回分子科学討論会
  • Place of Presentation: 東京
  • Year and Date: 2015-09-16
• [Presentation] 光駆動塩化物イオンポンプの反応初期中間体の発色団構造 (2015)
  • Author(s): 久保田 真司、水野 操、神取 秀樹、水谷 泰久
  • Organizer: 第9回分子科学討論会
  • Place of Presentation: 東京
  • Year and Date: 2015-09-16
• [Presentation] ナトリウムポンプ型ロドプシンの光反応中間体構造のカチオン依存性 (2015)
  • Author(s): 西村 尚、水野 操、神取 秀樹、水谷 泰久
  • Organizer: 第9回分子科学討論会
  • Place of Presentation: 東京
  • Year and Date: 2015-09-16
• [Presentation] Time-resolved resonance Raman observation of proteins in action (2015)
  • Author(s): 水谷泰久
  • Organizer: 日本生物物理学会第53回年会
  • Place of Presentation: 金沢
  • Year and Date: 2015-09-13
  • Invited
• [Presentation] Observation of rare events in retinal proteins revealed by time-resolved resonance Raman spectroscopy (2015)
  • Author(s): 水野操
  • Organizer: 日本生物物理学会第53回年会
  • Place of Presentation: 金沢
  • Year and Date: 2015-09-13
  • Invited
• [Presentation] Structural Changes of Microbial Rhodopsins in Their Photocycles as Revealed by Time-resolved Visible and Ultraviolet Resonance Raman Spectroscopy (2015)
  • Author(s): Yasuhisa Mizutani
  • Organizer: Telluride Science Research Center Workshop on Protein Dynamics
  • Place of Presentation: elluride, Colorado, USA
  • Year and Date: 2015-08-03
  • Int'l Joint Research / Invited
• [Presentation] Time-resolved Raman Mapping of Vibrational Energy Flow in Hemeproteins (2015)
  • Author(s): Yasuhisa Mizutani, Naoki Fujii, Masato Kondoh, Mitsuhiro Miyamoto, Misao Mizuno, and Haruto Ishikawa
  • Organizer: Seventeenth International Conference on Time-resolved Vibrational Spectroscopy
  • Place of Presentation: Madison, Wisconsin, USA
  • Year and Date: 2015-06-21
  • Int'l Joint Research
• [Presentation] Anisotropy of intraprotein vibrational energy transfer monitored by time-resolved anti-Stokes ultraviolet resonance Raman spectroscopy (2015)
  • Author(s): Masato Kondoh, Misao Mizuno and Yasuhisa Mizutani
  • Organizer: Seventeenth International Conference on Time-resolved Vibrational Spectroscopy
  • Place of Presentation: Madison, Wisconsin, USA
  • Year and Date: 2015-06-21
  • Int'l Joint Research
• [Presentation] Structural evolution of the retinal chromophore in the photocycle of microbial ion pumps (2015)
  • Author(s): Misao Mizuno, Ayumi Nakajima, Hideki Kandori, and Yasuhisa Mizutani
  • Organizer: Seventeenth International Conference on Time-resolved Vibrational Spectroscopy
  • Place of Presentation: Madison, Wisconsin, USA
  • Year and Date: 2015-06-21
  • Int'l Joint Research
• [Presentation] Effect of Intersubunit Interaction with an N-terminus Residue of the α Subunit on Dynamics of Human Hemoglobin (2015)
  • Author(s): Shanyan Chang, Misao Mizuno, Haruto Ishikawa and Yasuhisa Mizutani
  • Organizer: Seventeenth International Conference on Time-resolved Vibrational Spectroscopy
  • Place of Presentation: Madison, Wisconsin, USA
  • Year and Date: 2015-06-21
  • Int'l Joint Research
• [Presentation] Allosteric dynamics of hemoglobin revealed by time-resolved resonance Raman spectroscopy (2015)
  • Author(s): Yasuhisa Mizutani, Kenta Yamada, Haruto Ishikawa, Misao Mizuno
  • Organizer: 3rd Awaji International Workshop on “Electron Spin Science & Technology: Biological and Materials Science Oriented Applications
  • Place of Presentation: Awaji, Japan
  • Year and Date: 2015-06-14
  • Int'l Joint Research / Invited
• [Presentation] 時間分解紫外共鳴アンチストークスラマン分光法で観る蛋白質内エネルギー伝達における異方性 (2015)
  • Author(s): 近藤 正人、水野 操、水谷 泰久
  • Organizer: 日本化学会第94春季年会 平成27年3月27日～30日
  • Place of Presentation: 船橋
  • Year and Date: 2015-03-27
• [Presentation] Effect of intersubunit interaction with an N-terminus residue of the α subunit on dynamics of human hemoglobin (2015)
  • Author(s): Shanyan Chang, Misao Mizuno, Haruto Ishikawa, Yasuhisa Mizutani
  • Organizer: 日本化学会第94春季年会
  • Place of Presentation: 船橋
  • Year and Date: 2015-03-27
• [Presentation] 新規塩化物イオンポンプの発色団水素結合 (2015)
  • Author(s): 久保田 真司、水野 操、神取 秀樹、水谷 泰久
  • Organizer: 日本化学会第94春季年会
  • Place of Presentation: 船橋
  • Year and Date: 2015-03-27
• [Presentation] Structure changes of microbial rhodopsins in their photocycles as revealed by time-resolved visible and ultraviolet resonance Raman spectroscopy (2014)
  • Author(s): Yasuhisa Mizutani
  • Organizer: 16th International Conference on Retinal Proteins
  • Place of Presentation: Nagahama, Japan
  • Year and Date: 2014-10-06
  • Int'l Joint Research / Invited
• [Presentation] Time-resolved resonance Raman study on structural evolution of chromophore in halorhodopsin from Natoronobacterium pharaonis (2014)
  • Author(s): Misao Mizuno, Ayumi Nakajima, Hideki Kandori, Yasuhisa Mizutani
  • Organizer: 16th International Conference on Retinal Proteins
  • Place of Presentation: Nagahama, Japan
  • Year and Date: 2014-10-06
  • Int'l Joint Research
• [Presentation] タンパク質の機能を生み出す柔らかさの時間分解観測 (2014)
  • Author(s): 水谷泰久
  • Organizer: 日本生物物理学会第52回年会
  • Place of Presentation: 札幌
  • Year and Date: 2014-09-25
  • Invited
• [Presentation] タンパク質を基盤とした酸素濃度FRET センサー (2014)
  • Author(s): 石川 春人、青野重利、水谷 泰久
  • Organizer: 日本生物物理学会第52回年会
  • Place of Presentation: 札幌
  • Year and Date: 2014-09-25
• [Presentation] 細胞内の遊離ヘムの可視化に向けた融合タンパク質の開発 (2014)
  • Author(s): 松本和之、石川春人、青野重利、水谷泰久
  • Organizer: 日本生物物理学会第52回年会
  • Place of Presentation: 札幌
  • Year and Date: 2014-09-25
• [Presentation] タンパク質内エネルギー散逸のラマン時空間マッピング (2014)
  • Author(s): 水谷泰久
  • Organizer: 第8回分子科学討論会
  • Place of Presentation: 東広島
  • Year and Date: 2014-09-21
  • Invited
• [Presentation] 共鳴ラマン分光法による蛍光タンパク質Dronpaの発色団構造決定 (2014)
  • Author(s): 東野飛鳥、水野操、水谷泰久
  • Organizer: 第8回分子科学討論会
  • Place of Presentation: 東広島
  • Year and Date: 2014-09-21
• [Presentation] Time-resolved resonance Raman spectroscopy on retinal proteins (2014)
  • Author(s): Yasuhisa Mizutani
  • Organizer: 16th International Congress on Photobiology
  • Place of Presentation: Cordoba, Argentina
  • Year and Date: 2014-09-07
  • Int'l Joint Research / Invited
• [Presentation] Temporal Raman Mapping of Vibrational Energy Flow in Hemeproteins (2014)
  • Author(s): Yasuhisa Mizutani, Naoki Fujii, Mitsuhiro Miyamoto, Misao Mizuno, and Haruto Ishikawa
  • Organizer: 24th International Conference on Raman Spectroscopy
  • Place of Presentation: Jena, Germany
  • Year and Date: 2014-08-10
  • Int'l Joint Research
• [Presentation] Evolution of Chromophore Structure in Halorhodopsin Photointermediates (2014)
  • Author(s): Misao Mizuno, Ayumi Nakajima, Yumi Shimoo, Hideki Kandori, Yasuhisa Mizutani
  • Organizer: 24th International Conference on Raman Spectroscopy
  • Place of Presentation: Jena, Germany
  • Year and Date: 2014-08-10
  • Int'l Joint Research
• [Presentation] Proton Release and Uptake in Retinal Chromophore of Cyanobacterial Ion-pumping Rhodopsin Revealed by Time-resolved Resonance Raman Spectroscopy (2014)
  • Author(s): Ayumi Nakajima, Misao Mizuno, Hideki Kandori, Yasuhisa Mizutani
  • Organizer: 24th International Conference on Raman Spectroscopy
  • Place of Presentation: Jena, Germany
  • Year and Date: 2014-08-10
  • Int'l Joint Research
• [Presentation] Vibrational Energy Flow in Hemeproteins (2014)
  • Author(s): Yasuhisa Mizutani, Naoki Fujii, Mitsuhiro Miyamoto, Misao Mizuno, and Haruto Ishikawa
  • Organizer: 19th International Conference on Ultrafest Phenomena
  • Place of Presentation: Okinawa, Japan
  • Year and Date: 2014-07-07
  • Int'l Joint Research
• [Presentation] Spectroscopic Characterization of the Heme Detoxification Protein fromPlasmodium falciparum (2014)
  • Author(s): Haruto Ishikawa, Keisuke Nakatani, Shigetoshi Aono, Yasuhisa Mizutani
  • Organizer: 2nd Awaji International Workshop on “Electron Spin Science & Technology: Biological and Materials Science Oriented Applications
  • Place of Presentation: Awaji, Japan
  • Year and Date: 2014-06-15
  • Int'l Joint Research / Invited
• [Presentation] Investigation of the Heme Binding Site in α-glucosidase from Rhodnius prolixus (2014)
  • Author(s): Shotaro Kaku, Keisuke Nakatani, Haruto Ishikawa, Yasuhisa Mizutani
  • Organizer: 2nd Awaji International Workshop on “Electron Spin Science & Technology: Biological and Materials Science Oriented Applications
  • Place of Presentation: Awaji, Japan
  • Year and Date: 2014-06-15
  • Int'l Joint Research
• [Presentation] Protein dynamics of CooA upon CO dissociation studied by time-resolved resonance Raman spectroscopy (2013)
  • Author(s): 大友章裕、石川春人、水野操、青野重利、水谷泰久
  • Organizer: 日本生物物理学会第51回年会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2013-10-28 – 2013-10-30
• [Presentation] ハロロドプシン光反応中間体のタンパク質構造ダイナミクス観測 (2013)
  • Author(s): 水野操、下尾祐未、神取秀樹、水谷泰久
  • Organizer: 第7回分子科学討論会
  • Place of Presentation: 京都テルサ（京都府民総合交流プラザ）
  • Year and Date: 2013-09-24 – 2013-09-27
• [Presentation] 時間分解アンチストークス共鳴ラマン分光法によるタンパク質内振動エネルギー伝達の距離依存性の観測 (2013)
  • Author(s): 宮本光紘、水野操、石川春人、水谷泰久
  • Organizer: 第7回分子科学討論会
  • Place of Presentation: 京都テルサ（京都府民総合交流プラザ）
  • Year and Date: 2013-09-24 – 2013-09-27
• [Presentation] Observation of primary protein dynamics by ultraviolet resonance Raman spectroscopy (2013)
  • Author(s): Misao Mizuno
  • Organizer: 7th International Conference on Advanced Vibrational Spectroscopy
  • Place of Presentation: Kobe
  • Year and Date: 2013-08-25 – 2013-08-30
  • Invited
• [Presentation] "Propagations of displacement and energy in proteins (2013)
  • Author(s): Yasuhisa Mizutani
  • Organizer: Telluride Science Research Center Workshop on Protein Dynamics
  • Place of Presentation: Telluride, Colorado, USA
  • Year and Date: 2013-08-05 – 2013-08-09
  • Invited
• [Remarks] 大阪大学 大学院理学研究科 化学専攻 生物物理化学研究室
  • URL: http://www.chem.sci.osaka-u.ac.jp/lab/mizutani/index-jp.html
• [Remarks] 大阪大学大学院理学研究科 水谷研究室
  • URL: http://www.chem.sci.osaka-u.ac.jp/lab/mizutani/index-jp.html