| Budget Amount *help |
¥79,690,000 (Direct Cost: ¥61,300,000、Indirect Cost: ¥18,390,000)
Fiscal Year 2017: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2016: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2015: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2014: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2013: ¥25,090,000 (Direct Cost: ¥19,300,000、Indirect Cost: ¥5,790,000)
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| Outline of Final Research Achievements |
The faithful transmission of genetic information is fundamental to germline stem cell cycles. One exception to this is meiosis when active recombination takes place. How such opposing control of genome stability is coordinated with developmental programs is not well understood. Previous studies have shown that retinoic acid plays a key role in meiosis initiation in mammals. However, retinoic acid is a pleiotropic factor, which for example promotes neurogenesis and not meiosis in pluripotent embryonic stem cells. By developing a culture condition to induce mitosis-to-meiosis transition from germline stem cells and by carrying out multiomics analyses, we found that germline stem cells are primed to enter into meiosis at transcriptional and epigenetic levels independently of retinoic acid. This “meiosis priming” program exhibits characteristic cross-talk regulation with genome stability genes during the developmental cycle of germline cells.
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