| Budget Amount *help |
¥79,690,000 (Direct Cost: ¥61,300,000、Indirect Cost: ¥18,390,000)
Fiscal Year 2017: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2016: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2015: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2014: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2013: ¥25,090,000 (Direct Cost: ¥19,300,000、Indirect Cost: ¥5,790,000)
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| Outline of Final Research Achievements |
In order to elucidate the function of Piwi, we looked for Piwi-interacting proteins and discovered a factor named DmGTSF1. We discovered that DmGTSF1 is essential for retrotransposon silencing and importance of Zn-finger motif of DmGTSF1. In addition, we found that linker histone H1 also interacts with Piwi. Biochemical analyses revealed that Piwi positively regulates the interaction between target retrotransposons and H1. Furthermore, ATAC-seq method enabled us to conclude that Piwi promotes chromatin aggregation of target retrotransposons via H1. Based on the above results, a molecular mechanism of epigenome regulation by small RNA was addressed.
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