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Evolution of GSC regulation in salmonids

Planned Research

Project AreaMechanisms regulating gamete formation in animals
Project/Area Number 25114005
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionTokyo University of Marine Science and Technology

Principal Investigator

Yoshizaki Goro  東京海洋大学, 学術研究院, 教授 (70281003)

Project Period (FY) 2013-06-28 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥103,220,000 (Direct Cost: ¥79,400,000、Indirect Cost: ¥23,820,000)
Fiscal Year 2017: ¥21,450,000 (Direct Cost: ¥16,500,000、Indirect Cost: ¥4,950,000)
Fiscal Year 2016: ¥21,450,000 (Direct Cost: ¥16,500,000、Indirect Cost: ¥4,950,000)
Fiscal Year 2015: ¥21,450,000 (Direct Cost: ¥16,500,000、Indirect Cost: ¥4,950,000)
Fiscal Year 2014: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2013: ¥21,320,000 (Direct Cost: ¥16,400,000、Indirect Cost: ¥4,920,000)
Keywordsヒメマス / ニジマス / マスノスケ / 生殖細胞 / 代理親魚 / 生殖幹細胞 / 配偶子幹細胞 / サケ科 / 多回繁殖 / 一回繁殖 / 精原細胞 / サケ科魚類 / vasa / 生殖細胞移植 / サケ・マス
Outline of Final Research Achievements

Pacific salmon has both semelparous and iteroparous species. Rainbow trout, which is a primitive salmon shows iteroparity and maintained their type-A spermatogonia in their post-spermiated testes. Germ cell transplantation assay revealed that the type-A spermatogonia possessed stem cell population. On the other hand, sockeye salmon, which is an evolved species shows semelparity and did not possess any type-A spermatogonia in their post-spermiated testes. This change was continuous phenomenon and masu salmon and Chinook salmon, which are evolutionally positioned between above-mentioned two species possessed reproductive characteristics somewhere between rainbow trout and sockeye salmon. Germ cell transplantation study also revealed that the iteroparous rainbow trout produced gametes of sockeye salmon and Chinook salmon multiple times in their gonads.

Report

(6 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (100 results)

All 2018 2017 2016 2015 2014 2013

All Journal Article (31 results) (of which Int'l Joint Research: 13 results,  Peer Reviewed: 28 results,  Open Access: 4 results,  Acknowledgement Compliant: 4 results) Presentation (66 results) (of which Int'l Joint Research: 30 results,  Invited: 27 results) Book (3 results)

  • [Journal Article] Production of functional sperm by subcutaneous auto-grafting of immature testes in rainbow trout2018

    • Author(s)
      Hayashi Makoto、Sakuma Daika、Yoshizaki Goro
    • Journal Title

      Molecular Reproduction and Development

      Volume: 85 Issue: 2 Pages: 155-162

    • DOI

      10.1002/mrd.22949

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Production of live fish derived from frozen germ cells via germ cell transplantation2018

    • Author(s)
      Yoshizaki Goro、Lee Seungki
    • Journal Title

      Stem Cell Research

      Volume: 29 Pages: 103-110

    • DOI

      10.1016/j.scr.2018.03.015

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 代理親魚技法を用いた新たな養殖技術2018

    • Author(s)
      川村 亘、矢澤 良輔、濱崎 正臣、竹内 裕、吉崎 悟朗
    • Journal Title

      アグリバイオ

      Volume: 2 Pages: 12-15

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Stem cell activity of type A spermatogonia is seasonally regulated in rainbow trout2017

    • Author(s)
      Sato Mana、Hayashi Makoto、Yoshizaki Goro
    • Journal Title

      Biology of Reproduction

      Volume: 96 Issue: 6 Pages: 1303-1316

    • DOI

      10.1093/biolre/iox049

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Production of the medaka derived from vitrified whole testes by germ cell transplantation.2017

    • Author(s)
      Seki, S., Kusano, K., Lee, S., Iwasaki, Y., Yagisawa, M., Ishida, M., Hiratsuka, T., Sasado, T., Naruse, K., Yoshizaki, G.
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 43185-43185

    • DOI

      10.1038/srep43185

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Production of Tiger Puffer Takifugu rubripes Offspring from Triploid Grass Puffer Takifugu niphobles Parents2017

    • Author(s)
      Hamasaki Masaomi、Takeuchi Yutaka、Yazawa Ryosuke、Yoshikawa Souta、Kadomura Kazushi、Yamada Toshiyuki、Miyaki Kadoo、Kikuchi Kiyoshi、Yoshizaki Goro
    • Journal Title

      Marine Biotechnology

      Volume: 19 Issue: 6 Pages: 579-591

    • DOI

      10.1007/s10126-017-9777-1

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Application of dead end-knockout zebrafish to recipients of germ cell transplantation.2017

    • Author(s)
      Li Q, Fujii W, Naito K, Yoshizaki G.
    • Journal Title

      Molecular Reproduction and Development

      Volume: 84 Issue: 10 Pages: 1100-1111

    • DOI

      10.1002/mrd.22870

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Flow-cytometric enrichment of Pacific bluefin tuna type A spermatogonia based on light-scattering properties2017

    • Author(s)
      Ichida Kensuke、Kise Kazuyoshi、Morita Tetsuro、Yazawa Ryosuke、Takeuchi Yutaka、Yoshizaki Goro
    • Journal Title

      Theriogenology

      Volume: 101 Pages: 91-98

    • DOI

      10.1016/j.theriogenology.2017.06.022

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hypothermic storage of isolated spermatogonia and oogonia from rainbow trout ( Oncorhynchus mykiss )2017

    • Author(s)
      Falahatkar Bahram、Poursaeid Samaneh、Kitada Ryota、Yoshizaki Goro
    • Journal Title

      Cryobiology

      Volume: 76 Pages: 125-128

    • DOI

      10.1016/j.cryobiol.2017.03.005

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Efficient production of donor-derived gametes from triploid recipients following intra-peritoneal germ cell transplantation into a marine teleost, Nibe croaker (Nibea mitsukurii).2017

    • Author(s)
      Hiroyuki Yoshikawa, Yutaka Takeuchi, Yasuko Ino, Junjie Wang, Gaku Iwata, Naoki Kabeya, Ryosuke Yazawa, Goro Yoshizaki
    • Journal Title

      Aquaculture

      Volume: 478 Pages: 35-47

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Production of functionally sterile triploid Nibe croaker Nibea mitsukurii induced by cold-shock treatment with special emphasis on triploid aptitude as surrogate broodstock.2017

    • Author(s)
      Yutaka Takeuchi, Takashi Yatabe, Hiroyuki Yoshikawa, Yasuko Ino, Naoki Kabeya, Ryosuke Yazawa, Goro Yoshizaki
    • Journal Title

      Aquaculture

      Volume: in press

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Efficient production of donor-derived gametes from triploid recipients following intra-peritoneal germ cell transplantation into a marine teleost, Nibe croaker (Nibea mitsukurii)2016

    • Author(s)
      H. Yoshikawa, Y. Takeuchi, Y. Ino, J. Wang, G. Iwata, N. Kabeya, R. Yazawa, G. Yoshizaki
    • Journal Title

      Aquaculture

      Volume: - Pages: 35-47

    • DOI

      10.1016/j.aquaculture.2016.05.011

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Long-term (5 years) cryopreserved spermatogonia have high capacity to generate functional gametes via interspecies transplantation in salmonids.2016

    • Author(s)
      Seungki Lee, Yoshiko Iwasaki, Goro Yoshizaki
    • Journal Title

      Cryobiology

      Volume: 73 Issue: 2 Pages: 286-290

    • DOI

      10.1016/j.cryobiol.2016.08.001

    • NAID

      120006547602

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Characterization of a vasa homolog in the brown-marbled grouper (Epinephelus fuscoguttatus) and its expression in gonad and germ cells during larval development.2016

    • Author(s)
      Surintorn Boonanuntanasarn, Paiboon Bunlipatanon, Kensuke Ichida, Kirana Yoohat, Ornkanya Mengyu, Samart Detsathit, Ryosuke Yazawa, Goro Yoshizaki
    • Journal Title

      Fish Physiology and Biochemistry

      Volume: 42 Issue: 6 Pages: 1621-1636

    • DOI

      10.1007/s10695-016-0245-z

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Generation of juvenile rainbow trout derived from cryopreserved whole ovaries by intraperitoneal transplantation of ovarian germ cells.2016

    • Author(s)
      Seungki Lee, Naoto Katayama, Goro Yoshizaki
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 478(3) Pages: 1478-1483

    • NAID

      120006547604

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] 養殖産業における代理親魚技術の利用~飼育スペースや労力の軽減、育種の加速、生殖細胞バンクの構築など~2016

    • Author(s)
      矢澤良輔、竹内裕、吉崎悟朗
    • Journal Title

      月刊アクアネット

      Volume: 9 Pages: 40-43

    • NAID

      40020966751

    • Related Report
      2016 Annual Research Report
  • [Journal Article] 生殖細胞の凍結保存による絶滅危惧魚種の遺伝子資源の保存2016

    • Author(s)
      吉崎悟朗
    • Journal Title

      冷凍

      Volume: 91(1069) Pages: 15-20

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Production of germ cell-deficient salmonids by the dead end gene knockdown and their use as recipients for germ cell transplantation.2016

    • Author(s)
      Goro Yoshizaki, Kuniko Takashiba, Shotaro Shimamori, Kiyoko Fujinuma, Shinya Shikina, Tomoyuki Okutsu, Sachi Kume, Makoto Hayashi
    • Journal Title

      Molecular Reproduction and Development

      Volume: 83 Pages: 298-311

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Successful cryopreservation of spermatogonia in critically endangered Manchurian trout (Brachymystax lenok).2016

    • Author(s)
      Seungki Lee, Goro Yoshizaki
    • Journal Title

      Cryobiology

      Volume: 72 Pages: 165-168

    • NAID

      120006547603

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Germ Cell-Specific Excision of loxP-Flanked Transgenes in Rainbow Trout Oncorhynchus mykiss2016

    • Author(s)
      Katayama, N., Kume, S., Hattori-Ihara, S., Sadaie, S., Hayashi, M., Yoshizaki, G.
    • Journal Title

      Biology of Reproduction

      Volume: 94 Issue: 4 Pages: 1-12

    • DOI

      10.1095/biolreprod.115.136929

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Assessment of yellowtail kingfish (Seriola lalandi) as a surrogate host for the production of Southern bluefin tuna (Thunnus maccoyii)seed via spermatogonial germ cell transplantation2015

    • Author(s)
      Tetsuro Morita, Kagayaki Morishima, Misako Miwa, Naoki Kumakura, Satomi Kudo, Kensuke Ichida, Toru Mitsuboshi, Yutaka Takeuchi, and Goro Yoshizaki
    • Journal Title

      Marine Biotechnology

      Volume: 17 Issue: 5 Pages: 644-655

    • DOI

      10.1007/s10126-015-9657-5

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Assessment of yellowtail kingfish (Seriola lalandi) as a surrogate host for the production of Southern bluefin tuna (Thunnus maccoyii)seed via spermatogonial germ cell transplantation2015

    • Author(s)
      Ido Bar, Andre Smith, Erin Bubner, Goro Yoshizaki, Yutaka Takeuchi, Ryosuke Yazawa, Ben Nan Chen, Scott Cummins, Abigail Elizur
    • Journal Title

      Reproduction, Fertility and Development

      Volume: 0 Issue: 12 Pages: 0-0

    • DOI

      10.1071/rd15136

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Production of viable trout offspring derived from frozen whole fish.2015

    • Author(s)
      Seungki Lee, Shinsuke Seki, Naoto Katayama & Goro Yoshizaki
    • Journal Title

      Scientific Reports

      Volume: 5 Pages: 16045-16045

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Production of YY super male using spermatogonial transplantation into female recipients.2015

    • Author(s)
      Tomoyuki Okutsu1, Shinya Shikina2, Takashi Sakamoto3, Mamiko Mochizuki, and Goro Yoshizaki.
    • Journal Title

      Aquaculture

      Volume: in press

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Primordial germ cell migration in the yellowtail kingfish (Seriola lalandi) and identification of stromal cell-derived factor 1.2015

    • Author(s)
      Fernandez JA, Bubner EJ, Takeuchi Y, Yoshizaki G, Wang T, Cummins SF, Elizur A.
    • Journal Title

      Gen Comp Endocrinol.

      Volume: 213 Pages: 16-23

    • DOI

      10.1016/j.ygcen.2015.02.007

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Enrichment of spermatogonial stem cells using side population in teleost.2014

    • Author(s)
      Hayashi M, Sato M, Nagasaka Y, Sadaie S, Kobayashi S, Yoshizaki G.
    • Journal Title

      Biol Reprod

      Volume: 91 Pages: 23-23

    • DOI

      10.1016/j.domaniend.2014.06.004

    • NAID

      120006547607

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Expression patterns of gdnf and gfra1 in rainbow trout testis2014

    • Author(s)
      S. Nakajima, M. Hayashi, T. Kouguchi, K. Yamaguchi, M. Miwa, G. Yoshizaki
    • Journal Title

      Gene Expression Patterns

      Volume: 14(2) Issue: 2 Pages: 111-120

    • DOI

      10.1016/j.gep.2014.01.006

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression profile of ribosomal protein L10a throughout gonadal development2014

    • Author(s)
      W. Makkapan, G. Yoshizaki, M. Tashiro, W. Chotigeat
    • Journal Title

      Fish Physiology and Biochemistry

      Volume: online 03 Pages: 1-13

    • DOI

      10.1007/s10695-013-9906-3

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Production of genetically diversified fish seeds using spermatogonial transplantation2014

    • Author(s)
      M. Sato, T. Morita, N. Katayama, G. Yoshizaki
    • Journal Title

      Aquaculture

      Volume: 422-423 Pages: 218-224

    • DOI

      10.1016/j.aquaculture.2013.12.016

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intraperitoneal germ cell transplantation in the Nile tilapia Oreochromis niloticus2014

    • Author(s)
      R. Farlora, S. Hattori-Ihara, Y. Takeuchi, M. Hayashi, A. Octavera, Alimuddin, G. Yoshizaki
    • Journal Title

      Marine Biotechnology

      Volume: 16(3) Issue: 3 Pages: 309-320

    • DOI

      10.1007/s10126-013-9551-y

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification and migration of primordial germ cells in Atlantic salmon, Salmo salar: Characterization of vasa, dead end, and lymphocyte antigen 75 genes2013

    • Author(s)
      K. Nagasawa, G. Yoshizaki(他3名)
    • Journal Title

      Mol. Reprod. Dev

      Volume: 80巻 Issue: 2 Pages: 118-131

    • DOI

      10.1002/mrd.22142

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] サバ科宿主内でドナー由来クロマグロ生殖細胞を特異的に検出する抗体の作製2018

    • Author(s)
      窪川 つばさ、市田 健介、川村 亘、矢澤 良輔、吉崎 悟朗
    • Organizer
      平成30年度日本水産学会春季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ニジマス代理親を用いた一回繁殖型ヒメマス配偶子の複数回生産2018

    • Author(s)
      名村 峻、勘坂 弘治、吉崎 悟朗
    • Organizer
      平成30年度日本水産学会春季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] in vitro 培養したニジマス精原細胞からの機能的な卵・精子の作出2018

    • Author(s)
      岩崎 佳子、DM バルデス、藤原 亮、岡部 貴文、吉崎 悟朗
    • Organizer
      平成30年度日本水産学会春季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ゲノム編集により作出したアルビノスマは仔魚期におけるワムシ摂餌能が低い2018

    • Author(s)
      長谷川 二己、川村 亘、山内 章弘、矢澤 良輔、吉崎 悟朗
    • Organizer
      平成30年度日本水産学会春季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] サバ雑種の生殖腺はクロマグロ生殖細胞の増殖を支持する2018

    • Author(s)
      川村 亘、窪川 つばさ、市田 健介、矢澤 良輔、吉崎 悟朗
    • Organizer
      平成30年度日本水産学会春季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 生殖細胞欠損ニジマスの作出とその代理親魚への応用2018

    • Author(s)
      藤原 亮、片山 直人、藤井 渉、内藤 邦彦、吉崎 悟朗
    • Organizer
      平成30年度日本水産学会春季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] タイセイヨウサケ属魚類の精原細胞の可視化2018

    • Author(s)
      市田 健介、林 誠、吉崎 悟朗
    • Organizer
      平成30年度日本水産学会春季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 片側卵巣を摘出したニジマス、ヤマメの各種生殖細胞の挙動2017

    • Author(s)
      岡部貴文、吉崎悟朗
    • Organizer
      平成29年度日本水産学会春季大会
    • Place of Presentation
      東京都, 港区(東京海洋大学品川キャンパス)
    • Year and Date
      2017-03-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] タイヘイヨウサケ属魚類における精原幹細胞の消長とその制御要因の解明2017

    • Author(s)
      北田凌太、寺澤まどか、勘坂弘治、佐藤茉菜、吉崎悟朗
    • Organizer
      平成29年度日本水産学会春季大会
    • Place of Presentation
      東京都, 港区(東京海洋大学品川キャンパス)
    • Year and Date
      2017-03-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] 生殖細胞欠損ヤマメの作出とその代理親魚への応用2017

    • Author(s)
      藤原亮、片山直人、藤井渉(東大院農)、内藤邦彦(東大院農)、吉崎悟朗
    • Organizer
      平成29年度日本水産学会春季大会
    • Place of Presentation
      東京都, 港区(東京海洋大学品川キャンパス)
    • Year and Date
      2017-03-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] Production of Viable Trout Offspring Derived from Frozen Whole Fish2017

    • Author(s)
      Goro Yoshizaki
    • Organizer
      50th Annual Meeting of the Society for the Study of Reproduction
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] TRANSPLANTATION OF SALMON GERM CELLS INTO RAINBOW TROUT RECIPIENTS:CAN ITEROPAROUS TROUT REPEATEDLY PRODUCE GAMETES DERIVED FROM SEMELPAROUS SALMON?2017

    • Author(s)
      Goro Yoshizaki
    • Organizer
      The International Research Symposium on Regulation of Germ Cell Development in vivo and in vitro
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Characterization of Germ-Line Stem Cells by Single-Cell Transcriptome Analysis in Rainbow Trout2017

    • Author(s)
      Goro Yoshizaki
    • Organizer
      The International Plant and Animal Genome Conference(PAG) ASIA 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Production of viable trout offspring derived from germ cells cultured in vitro2017

    • Author(s)
      Goro Yoshizaki
    • Organizer
      The 11th Asia Pacific Marine Biotechnology Conference (APMBC)2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Cryopreservation of rainbow trout whole gonads by vitrification to maintain reproductive stem cell potential2017

    • Author(s)
      Tawny Nicole Aiko Scanlan, Goro Yoshizaki, Stuart Meyers
    • Organizer
      Fourth World Congress of Reproductive Biology (WCRB2017) (国際生殖生物学会)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Cryopreservation of spermatogonial stem cells from cyprinid fish species2017

    • Author(s)
      Zoran Marinovic, Jelena Lujic, Eszter Kasa, Qian Li, Goro Yoshizaki, Roman Franek, Vojtech Kaspar, Bela Urbanyi, Akos Horvath
    • Organizer
      Fourth World Congress of Reproductive Biology (WCRB2017) (国際生殖生物学会)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Hybrid mackerel (Scomber australasicus × S. japonicus) possesses germ cell-deficient sterile gonads: Its suitability as a surrogate recipient for gamete production of bluefin tuna2017

    • Author(s)
      Wataru Kawamura, Ryosuke Yazawa, Reoto Tani, Yutaka Takeuchi, Goro Yoshizaki
    • Organizer
      Fourth World Congress of Reproductive Biology (WCRB2017) (国際生殖生物学会)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Production of viable trout offspring derived from germ cells cultured in vitro2017

    • Author(s)
      Yoshiko Iwasaki, Goro Yoshizaki
    • Organizer
      Fourth World Congress of Reproductive Biology (WCRB2017) (国際生殖生物学会)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Application of dead end-knockout zebrafish to recipients of germ cell transplantation2017

    • Author(s)
      Qian Li, Wataru Fujii, Kunihiko Naito, Goro Yoshizaki
    • Organizer
      Fourth World Congress of Reproductive Biology (WCRB2017) (国際生殖生物学会)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Identification of the molecular markers preferentially expressed in spermatogonial stem cells in fish2017

    • Author(s)
      Makoto Hayashi, Yoshiko Iwasaki, Tetsutaro Hayashi, Masashi Ebisawa, Yohei Sasagawa, Mika Yoshimura, Kensuke Ichida, Itoshi Nikaido, Goro Yoshizaki
    • Organizer
      Fourth World Congress of Reproductive Biology (WCRB2017) (国際生殖生物学会)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] FIRST EVIDENCE IN FISH OF ALTERNATIVE DEAD END GENE SPLICING2017

    • Author(s)
      Ana Carina Nogueira Vasconcelos, Anna Octavera, Danilo Pedro Streit Jr., Goro Yoshizaki
    • Organizer
      6th International Workshop on the Biology of Fish Gametes(第6回魚類配偶子国際会議)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] CRYOPRESERVATION OF ZEBRAFISH SPERMATOGONIA: SLOW-RATE FREEZING VS VITRIFICATION2017

    • Author(s)
      Zoran Marinovic, Qian Li, Jelena Lujic, Eszter Kasa, Yoshiko Iwasaki, Bela Urbanyi, Akos Horvath, Goro Yoshizaki
    • Organizer
      6th International Workshop on the Biology of Fish Gametes(第6回魚類配偶子国際会議)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Production of rainbow trout offspring derived from germ cells cultured in vitro2017

    • Author(s)
      Yoshiko Iwasaki, Goro Yoshizaki
    • Organizer
      日本水産学会創立85周年記念国際シンポジウム International Symposium“Fisheries Science for Future Generations”The Japanese Society of Fisheries Science
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Specific visualization of type A spermatogonia using fluorescence-conjugated antibody-recognizing cell surface antigen in Pacific bluefin tuna2017

    • Author(s)
      Kensuke Ichida, Wataru Kawamura, Kanta Mori, Makoto Hayashi, Ryosuke Yazawa, Goro Yoshizaki
    • Organizer
      日本水産学会創立85周年記念国際シンポジウム International Symposium“Fisheries Science for Future Generations”The Japanese Society of Fisheries Science
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Production of Kyushu bitterling offspring from Chinese rosy bitterling recipients using spermatogonial transplantation2017

    • Author(s)
      Anna Octavera, Goro Yoshizaki
    • Organizer
      日本水産学会創立85周年記念国際シンポジウム International Symposium“Fisheries Science for Future Generations”The Japanese Society of Fisheries Science
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of a novel immunosenser system for fish sex determination2017

    • Author(s)
      Haiyun Wu, Taro Sakurai, Goro Yoshizaki, Hitoshi Ohnuki, Hideaki Endo
    • Organizer
      日本水産学会創立85周年記念国際シンポジウム International Symposium“Fisheries Science for Future Generations”The Japanese Society of Fisheries Science
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Sex Determination and Downstream Events Associated with Gonadal Sex Differentiation in Sablefish2017

    • Author(s)
      J.Adam Luckenbach, Edward S.Hayman, Goro Yoshizaki, Anna Octavera, William T.Fairgrieve
    • Organizer
      50th Annual Meeting of the Society for the Study of Reproduction
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Production of Kyushu bitterling offspring from Chinese rosy bitterling recipients using spermatogonial transplantation2017

    • Author(s)
      Anna Octavera
    • Organizer
      The International Research Symposium on Regulation of Germ Cell Development in vivo and in vitro
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 代理親魚技法の構築とその応用に関する研究2017

    • Author(s)
      吉崎 悟朗
    • Organizer
      平成29年度(第88回)日本農学大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] クロマグロ精原細胞の可視化2017

    • Author(s)
      市田 健介、川村 亘、林 誠、矢澤 良輔、吉崎 悟朗
    • Organizer
      第19回 マリンバイオテクノロジー学会 仙台大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Production of Viable Trout Offspring Derived from Frozen Testis Via Germ Cell Transplantation.2017

    • Author(s)
      Goro Yoshizaki
    • Organizer
      Cryopreservation of Aquatic Biomedical Models Workshop
    • Place of Presentation
      U.S.A. Birmingham, Alabama(University of Alabama at Birmingham)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 生殖細胞を借り腹でつくる ~マグロを絶滅から救えるのか?~2017

    • Author(s)
      吉崎悟朗
    • Organizer
      日本動物学会第69回関東支部大会 公開シンポジウム『生殖細胞に秘められたパワーを解く』
    • Place of Presentation
      東京都, 文京区(筑波大学 東京キャンパス文京校舎)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Germ-cell transplantation research:collaboration between a university and a fishery company.2016

    • Author(s)
      Goro Yoshizaki
    • Organizer
      The 15th Asia Maritime & Fisheries Universities Forum
    • Place of Presentation
      台湾, 基隆市(National Taiwan Ocean University)
    • Year and Date
      2016-10-13
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Production of donor-derived offspring by allogeneic tranplantation of spermatogonia in Chinese rosy bitterling.2016

    • Author(s)
      Anna Octavera、吉崎悟朗
    • Organizer
      平成28年度日本水産学会秋季大会
    • Place of Presentation
      奈良県, 奈良市(近畿大学農学部奈良キャンパス)
    • Year and Date
      2016-09-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] Cloning of Dead end gene in Colossoma macropomum and its expression in the germ cell lineage.2016

    • Author(s)
      Ana Carina Nogueira Vasconcelos(UFRGS-Brazil,TUMAST)、Anna Octavera、Goro Yoshizaki
    • Organizer
      平成28年度日本水産学会秋季大会
    • Place of Presentation
      奈良県, 奈良市(近畿大学農学部奈良キャンパス)
    • Year and Date
      2016-09-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] TREATMENT OF DISSOCIATED GERM CELLS WITH RHO KINASE.2016

    • Author(s)
      Iwasaki Yoshiko, Goro Yoshizaki
    • Organizer
      IMBC2016(マリンバイオテクノロジー国際学会)
    • Place of Presentation
      Baltimore, Maryland, USA(Hyatt Regency Inner Harbor)
    • Year and Date
      2016-08-29
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] REPEATED PRODUCTION OF SEMELPAROUS CHINOOK SALOMON GAMETES USING SURROGATE RAINBOW TROUT.2016

    • Author(s)
      Goro Yoshizaki
    • Organizer
      IMBC2016(マリンバイオテクノロジー国際学会)
    • Place of Presentation
      Baltimore, Maryland, USA(Hyatt Regency Inner Harbor)
    • Year and Date
      2016-08-29
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Germ cell manipulation in fish.2016

    • Author(s)
      Goro Yoshizaki
    • Organizer
      The 22th Japanese medaka and zebrafish meeting
    • Place of Presentation
      Okazaki, Japan (National Institute for Basic Biology)
    • Year and Date
      2016-08-20
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Production of viable trout offspring derived from frozen whole fish.2016

    • Author(s)
      Goro Yoshizaki
    • Organizer
      The 9th NIBB International Practical Course
    • Place of Presentation
      Okazaki, Japan (National Institute for Basic Biology)
    • Year and Date
      2016-08-13
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Germ cell manipulation in fish.2016

    • Author(s)
      Goro Yoshizaki
    • Organizer
      19th European Testis Workshop
    • Place of Presentation
      France, Saint-Malo(Palais du Grand Large)
    • Year and Date
      2016-06-11
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Germ Cell Manipulation in Fish: Can Mackerel Produce Tuna Gametes?2016

    • Author(s)
      Goro Yoshizaki
    • Organizer
      The 7th World Fisheries Congress, Busan 2016 Secretariat
    • Place of Presentation
      韓国, 釜山(The Busan Exhibition & Convention Center (BEXCO))
    • Year and Date
      2016-05-23
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] サバからマグロを産ませる2016

    • Author(s)
      吉崎悟朗
    • Organizer
      生殖生物学シンポジウム『生殖戦略を支える細胞たち』
    • Place of Presentation
      京都
    • Year and Date
      2016-02-22
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Present status of germ cell transplantation for conservation in fish.2016

    • Author(s)
      Goro Yoshizaki
    • Organizer
      1st International Conference on Aquaculture Biotechnology 2016
    • Place of Presentation
      Bogor, Indonesia(IPB International Convention Center)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] GERM CELL TRANSPLANTATION IN FISH: CAN SURROGATE MACKEREL MAKE TUNA?2015

    • Author(s)
      G. Yoshizaki
    • Organizer
      AQUATIC PRODUCTS PROCESSING: Cleaner Production Chain for Healthier Food
    • Place of Presentation
      CanTho Vietnam
    • Year and Date
      2015-12-07
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] PRODUCTION OF VIABLE TROUT OFFSPRING DERIVED FROM FROZEN WHOLE FISH2015

    • Author(s)
      G. Yoshizaki
    • Organizer
      The 5th International Workshop on the Biology of Fish Gametes
    • Place of Presentation
      Ancona Italy
    • Year and Date
      2015-09-07
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Germ cell transplantation in fish: can mackerel make tuna?2015

    • Author(s)
      G. Yoshizaki
    • Organizer
      デンマーク養殖アカデミックワークショップ
    • Place of Presentation
      東京
    • Year and Date
      2015-09-03
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 一回繁殖型サケの配偶子を多回繁殖型サケに何度も作らせる2015

    • Author(s)
      勘坂弘治・島森翔大郎・寺澤まどか・神谷賢治・Delgad M. VALDEZ JR.・定家咲子・吉崎悟朗
    • Organizer
      平成27年度日本水産学会春季大会
    • Place of Presentation
      東京都、港区 (東京海洋大学品川キャンパス)
    • Year and Date
      2015-03-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] 雌メダカを氷冷すると放卵する2015

    • Author(s)
      草野和成・岩崎佳子・市田健介・吉崎悟朗
    • Organizer
      平成27年度日本水産学会春季大会
    • Place of Presentation
      東京都、港区 (東京海洋大学品川キャンパス)
    • Year and Date
      2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 分子レベルでのニジマスA型精原細胞の細分化;nanos2を指標として2015

    • Author(s)
      林 誠・小野澤健明・定家咲子・吉崎悟朗
    • Organizer
      平成27年度日本水産学会春季大会
    • Place of Presentation
      東京都、港区 (東京海洋大学品川キャンパス)
    • Year and Date
      2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] ゴマサバ♀・マサバ♂雑種は成長過程で生殖細胞が消失する2015

    • Author(s)
      川村 亘・矢澤良輔・雨澤孝太朗・谷 怜央人・竹内 裕・吉崎悟朗
    • Organizer
      平成27年度日本水産学会春季大会
    • Place of Presentation
      東京都、港区 (東京海洋大学品川キャンパス)
    • Year and Date
      2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] 「サバからマグロは産まれるか」2014

    • Author(s)
      吉崎 悟朗
    • Organizer
      読売テクノ・フォーラム 2014年度 秋のシンポジウム ゴールド・メダル賞創設20周年を記念する講演会 科学の力で、世界を元気に
    • Place of Presentation
      東京都千代田区(読売新聞東京本社 よみうり大手町ホール)
    • Year and Date
      2014-11-26
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 魚類の生殖細胞操作:サバからマグロは生まれるか?2014

    • Author(s)
      吉崎 悟朗
    • Organizer
      名古屋大学 グリーン自然科学国際教育研究プログラム 『第27回IGERグリーン自然科学レクチャー』
    • Place of Presentation
      愛知県名古屋市(名古屋大学 ES総合館ESホール)
    • Year and Date
      2014-07-25
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Transplantation of salmon germ cells intro rainbow trout recipients: can iteroparous trout repeatedly produce gametes derived from semelparous salmon?2014

    • Author(s)
      G. Yoshizaki
    • Organizer
      10th International Symposium on Reproductive Physiology of Fish
    • Place of Presentation
      Olhão,Faro,Portuguese Republic(Olhão Municipal Auditorium)
    • Year and Date
      2014-05-27
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Mass production of sterile fish: how can we produce gametes from sterile fish?2014

    • Author(s)
      G. Yoshizaki
    • Organizer
      The 10th Asia-Pacific Marine Biotechnology Conference
    • Place of Presentation
      Taipei, Taiwan(Academia Sinica)
    • Year and Date
      2014-05-05
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Development of surrogate broodstock technology.2014

    • Author(s)
      G. Yoshizaki
    • Organizer
      SATREPS Annual Meeting 2014
    • Place of Presentation
      Bangkok, Thailand (Centara Grand at Central Plaza Ladprao)
    • Year and Date
      2014-04-26
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Production of donor-derived eggs and sperm by allogenic transplantation of cryopreserved spermatogonia in medaka.2014

    • Author(s)
      G. Yoshizaki
    • Organizer
      2nd Strategical Meeting for Medaka Research
    • Place of Presentation
      Seville, Spain, (Spanish Research Council (CSIC) museum “Casa de la Ciencia”).
    • Year and Date
      2014-04-11
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 超雄由来精原細胞の移植によるY精子のみを生産する代理親魚の作出2014

    • Author(s)
      片山直人、佐藤茉菜、識名信也、定家咲子、吉崎悟朗
    • Organizer
      平成26年度公益社団法人日本水産学会春季大会
    • Place of Presentation
      北海道、函館市(北海道大学)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ニジマス精原細胞を濃縮するための細胞表面マーカーの単離2014

    • Author(s)
      林誠、定家咲子、長坂安彦、吉崎悟朗
    • Organizer
      平成26年度公益社団法人日本水産学会春季大会
    • Place of Presentation
      北海道、函館市(北海道大学)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 凍結イワナ精巣からの機能的な卵、精子の作出2014

    • Author(s)
      Lee Seungki, 吉崎悟朗
    • Organizer
      平成26年度公益社団法人日本水産学会春季大会
    • Place of Presentation
      北海道、函館市(北海道大学)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 解離精原細胞に対するRhoキナーゼ (ROCK)インヒビター処理は宿主への移植効率を改善する2014

    • Author(s)
      岩崎佳子、吉崎悟朗
    • Organizer
      平成26年度公益社団法人日本水産学会春季大会
    • Place of Presentation
      北海道、函館市(北海道大学)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 磁気細胞分離法によるニジマス生殖細胞の濃縮2014

    • Author(s)
      市田健介、林誠、吉崎悟朗
    • Organizer
      平成26年度公益社団法人日本水産学会春季大会
    • Place of Presentation
      北海道、函館市(北海道大学)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 魚類の生殖細胞操作:サバからマグロは生まれるか?2014

    • Author(s)
      吉崎 悟朗
    • Organizer
      広島大学 大学院生物圏科学研究科シンポジウム『生物学の基礎的研究から水・畜産業へのトランスレーション 』
    • Place of Presentation
      広島県 東広島市(広島大学大学院生物生産学部)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] ニジマス生殖細胞における高効率な遺伝子編集システムの開発2013

    • Author(s)
      片山直人、久米佐知、ハットリ-伊原祥子、定家咲子、吉崎悟朗
    • Organizer
      日本動物遺伝育種学会第14回大会
    • Place of Presentation
      東京都港区(東京海洋大学品川キャンパス)
    • Related Report
      2013 Annual Research Report
  • [Presentation] サバにマグロを生ませる2013

    • Author(s)
      吉崎悟朗
    • Organizer
      高知大学アカデミアセミナー 「海洋」その恵み・神秘・脅威
    • Place of Presentation
      高知県高知市(高新RKCホール)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 魚類の生殖細胞移植:現状とその可能性2013

    • Author(s)
      吉崎悟朗
    • Organizer
      マリンバイオテクノロジー学会懇談会
    • Place of Presentation
      東京都新宿区(早稲田大学先端生命医科学センター)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Germ cell-specific excision of the loxP-flanked transgene in rainbow trout2013

    • Author(s)
      N. Katayama, S. Kume, S. Sadaie, S. Ihara and G. Yoshizaki
    • Organizer
      The 10th International Marine Biotechnology Conference 2013
    • Place of Presentation
      Brisbane, Australia (Brisbane Convention and Exhibition Centre)
    • Related Report
      2013 Annual Research Report
  • [Book] 水産遺伝育種学 「第12章 遺伝子・細胞操作と水産育種における応用」2017

    • Author(s)
      片山 直人、壁谷 尚樹、竹内 裕、矢澤 良輔、吉崎 悟朗
    • Total Pages
      256
    • Publisher
      東北大学出版会
    • ISBN
      9784861632709
    • Related Report
      2017 Annual Research Report
  • [Book] ラボ必携 フローサイトメトリーQ&A 「Q95 魚類(サケ・マス類,マグロ類,ゼブラフィッシュやメダカなど)から調製した細胞のフローサイトメトリー解析やソーティングは可能でしょうか?」2017

    • Author(s)
      林 誠、市田 健介、吉崎 悟朗
    • Total Pages
      312
    • Publisher
      羊土社
    • ISBN
      9784758122351
    • Related Report
      2017 Annual Research Report
  • [Book] サバからマグロが産まれる!?2014

    • Author(s)
      吉崎 悟朗
    • Total Pages
      126
    • Publisher
      岩波書店
    • Related Report
      2014 Annual Research Report

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Published: 2013-07-10   Modified: 2019-07-29  

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