|Budget Amount *help
¥130,130,000 (Direct Cost: ¥100,100,000、Indirect Cost: ¥30,030,000)
Fiscal Year 2017: ¥26,390,000 (Direct Cost: ¥20,300,000、Indirect Cost: ¥6,090,000)
Fiscal Year 2016: ¥26,520,000 (Direct Cost: ¥20,400,000、Indirect Cost: ¥6,120,000)
Fiscal Year 2015: ¥26,390,000 (Direct Cost: ¥20,300,000、Indirect Cost: ¥6,090,000)
Fiscal Year 2014: ¥22,880,000 (Direct Cost: ¥17,600,000、Indirect Cost: ¥5,280,000)
Fiscal Year 2013: ¥27,950,000 (Direct Cost: ¥21,500,000、Indirect Cost: ¥6,450,000)
|Outline of Final Research Achievements
In this study, we successfully established an in vitro system that produces mature oocytes from mouse primordial germ cells (PGC), for the first time.
The main challenge which we had to overcome was abnormal follicle formation in vitro. To understand the cause of abnormal follicle formation, RNA-seq analysis was conducted in the explants. We found that the estrogen pathway was excessively activated in the cultured ovaries. Therefore, an antagonist of estrogen receptor was added to the medium. This resulted in secondary follicle formation in the cultured ovary. The isolated secondary follicles were cultured in the medium supplemented with macromolecular compounds. Addition of macromolecular compounds to the medium increased the number of matured oocytes. These mature oocytes were able to develop to offspring after fertilization and embryo transfer. In the future, this in vitro system will be useful tools which assist in understanding oogenesis and producing a lot of mammalian oocytes.