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酸素受容・活性化に伴うリガンドシグナルの生成と制御

Planned

Project AreaOxygen biology: a new criterion for integrated understanding of life
Project/Area Number 26111011
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionThe University of Tokyo (2015-2018)
Nagoya University (2014)

Principal Investigator

内田 浩二  東京大学, 大学院農学生命科学研究科(農学部), 教授 (40203533)

Co-Investigator(Kenkyū-buntansha) 西田 基宏  大学共同利用機関法人自然科学研究機構(新分野創成センター、アストロバイオロジーセンター、生命創成探究, 生命創成探究センター, 教授 (90342641)
居原 秀  大阪府立大学, 理学(系)研究科(研究院), 准教授 (60254447)
柴田 貴広  名古屋大学, 生命農学研究科, 准教授 (80447838)
Project Period (FY) 2014-07-10 – 2019-03-31
Project Status Granted (Fiscal Year 2018)
Budget Amount *help
¥113,750,000 (Direct Cost: ¥87,500,000、Indirect Cost: ¥26,250,000)
Fiscal Year 2018: ¥22,230,000 (Direct Cost: ¥17,100,000、Indirect Cost: ¥5,130,000)
Fiscal Year 2017: ¥22,230,000 (Direct Cost: ¥17,100,000、Indirect Cost: ¥5,130,000)
Fiscal Year 2016: ¥22,490,000 (Direct Cost: ¥17,300,000、Indirect Cost: ¥5,190,000)
Fiscal Year 2015: ¥22,360,000 (Direct Cost: ¥17,200,000、Indirect Cost: ¥5,160,000)
Fiscal Year 2014: ¥24,440,000 (Direct Cost: ¥18,800,000、Indirect Cost: ¥5,640,000)
Keywords活性酸素種 / ケミカルバイオロジー / タンパク質修飾 / リモデリング / レドックス
Outline of Annual Research Achievements

1.生体内酸化修飾分子の網羅的解析:LC-ESI/MS/MSを用いて、マウス個体、培養細胞における、酸化修飾イミダゾールジペプチドの産生と酸化ストレスとの関連性を明らかにした。また、安定同位体酸素、ラジカル補足剤を用いて酸化修飾の分子機構を明らかにした。
2.酸素活性化を起源とするタンパク質酸化修飾による生体防御応答機構の解析:タンパク質をピロール化する内因性因子の同定を目標に、糖質および脂質などについてスクリーニングを行なった結果、脂質過酸化生成物であり、解糖系分子でもあるグリコールアルデヒドを同定した。一方、植物性小分子酸素センサーであるポリフェノール類に関して、抗酸化剤の作用により生成するタンパク質カルボニルが自然抗体のエピトープとして認識される作用機構について詳細に解析した結果、タンパク質表面の陰性荷電がその認識に関わっていることが推測された。
3.タンパク質酸化修飾を基軸にした修復・再生応答機構の解明:ホモシステイン化アルブミンの研究では、ホモシステイン化が遊離SH基だけでなく、ジスルフィド結合にも生じるという画期的発見に至った。心筋が低酸素環境下で活性酸素を生成する機構の解析の結果、アントラサイクリン系抗がん剤は心筋細胞に多く含まれる酸素貯蔵タンパク質ミオグロビンから鉄を引き抜き、貯蔵酸素を解離させることで低酸素ストレスを誘発することを見出した。また、低酸素誘導性のTRPC3チャネルタンパク質がNADPH酸化酵素(NOX2)を複合体形成依存的に安定化し、増加したNOX2が活性酸素を生成することで、心筋萎縮を誘発することをマウスで明らかにした。

Current Status of Research Progress
Current Status of Research Progress

1: Research has progressed more than it was originally planned.

Reason

生体内酸化修飾分子の網羅的解析では、イミダゾールジペプチドの2-oxo化の分子機構の解明に到達している。オリジナリティの高いタンパク質のピロール化では、内因性のピロール化因子を同定しており、酸素に起因する修飾タンパク質の生物学的意義の解明につながる可能性を示唆する。また、血清アルブミンのジスルフィド結合に対するホモシステイン化は画期的発見であり、SH化合物のリザーバーとしての血清アルブミンの新たな機能性の解明につながるものと期待される。

Strategy for Future Research Activity

最終年度にあたり、成果発表(論文)を念頭に以下の項目について重点的に進める。
1.酸化修飾イミダゾールジペプチドが、シグナル分子として機能しているかどうかを様々なモデル実験系で検証する。また、酸化修飾に関連するニトロ化、メルカプト化など他の修飾分子の探索、他の化合物(タンパク質など)中での修飾イミダゾール基の探索を行う。
2.グリコールアルデヒドによるピロール化機構について詳細に解析するとともに、脾細胞などを用い、ピロール化タンパク質による自然免疫応答の分子機構を解析する。
3.活性酸素による心筋の可塑性にはミトコンドリア分裂が関与しており、そのメカニズムとしてdynamin-related protein 1 (Drp1)タンパク質C末端にあるシステイン修飾が関与することも見出している。今後は、Drp1のシステイン修飾様式とDrp1活性との関連を明らかにし、そこを特異的に阻害することで心疾患リスクが軽減されることを検証する。

Report

(4 results)
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report

Research Products

(69 results)

All 2018 2017 2016 2015 2014

All Journal Article Presentation Book

  • [Journal Article] Identification of Polyphenol-Specific Innate Epitopes That Originated from a Resveratrol Analogue2018

    • Author(s)
      Furuhashi, M., Hatasa, Y., Kawamura, S., Shibata, T., Akagawa, M., Uchida, K.
    • Journal Title

      Sci. Rep.

      Volume: 56 Pages: 470-4712

    • DOI

      10.1021/acs.biochem.7b00409

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 8-Nitro-cGMP Attenuates the Interaction between SNARE Complex and Complexin through S-Guanylation of SNAP-252018

    • Author(s)
      Kishimoto Y, Kunieda K, Kitamura A, Kakihana Y, Akaike T, Ihara H.
    • Journal Title

      ACS chemical neuroscience

      Volume: 9 Pages: 217-223

    • DOI

      10.1021/acschemneuro.7b00363

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Involvement of nitric oxide/reactive oxygen species signaling via 8-nitro-cGMP formation in 1-methyl-4-phenylpyridinium ion-induced neurotoxicity in PC12 cells and rat cerebellar granule neurons.2018

    • Author(s)
      Masuda Kumiko、Tsutsuki Hiroyasu、Kasamatsu Shingo、Ida Tomoaki、Takata Tsuyoshi、Sugiura Kikuya、Nishida Motohiro、Watanabe Yasuo、Sawa Tomohiro、Akaike Takaaki、Ihara Hideshi
    • Journal Title

      Biochemical and biophysical research communications

      Volume: 495 Pages: 2165-2170

    • DOI

      10.1016/j.bbrc.2017.12.088

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] TRPC3 participates in angiotensin II type 1 receptor-dependent stress-induced slow increase in intracellular Ca2+ concentration in mouse cardiomyocytes.2018

    • Author(s)
      Yamaguchi Y, Iribe G, Kaneko T, Takahashi K, Numaga-Tomita T, Nishida M, Birnbaumer L, Naruse K.
    • Journal Title

      J Physiol Sci

      Volume: 印刷中 Pages: 1-12

    • DOI

      10.1007/s12576-016-0519-3

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Adductome-based identification of biomarkers for lipid peroxidation.2017

    • Author(s)
      Shibata T, Shimizu K, Hirano K, Nakashima F, Kikuchi R, Matsushita T, Uchida K.
    • Journal Title

      J. Biol. Chem.

      Volume: 292(20) Pages: 8223-8235

    • DOI

      10.1074/jbc.m116.762609

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Roles of 5-lipoxygenase and cyclooxygenase-2 in the biosynthesis of hemiketals E2 and D2 by activated human leukocytes2017

    • Author(s)
      Gimenez-Bastida JA, Shibata T, Uchida K, Schneider C.
    • Journal Title

      FASEB J.

      Volume: 31 Pages: 1867-1878

    • DOI

      10.1096/fj.201601136r

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] TRPC3-Nox2 complex mediates doxorubicin-induced myocardial atrophy.2017

    • Author(s)
      Shimauchi Tsukasa、Numaga-Tomita Takuro、Ito Tomoya、Nishimura Akiyuki、Matsukane Ryosuke、Oda Sayaka、Hoka Sumio、Ide Tomomi、Koitabashi Norimichi、Uchida Koji、Sumimoto Hideki、Mori Yasuo、Nishida Motohiro
    • Journal Title

      JCI Insight.

      Volume: 2 Pages: 1-10

    • DOI

      10.1172/jci.insight.93358

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] TRPC6 counteracts TRPC3-Nox2 protein complex leading to attenuation of hyperglycemia-induced heart failure in mice.2017

    • Author(s)
      Oda Sayaka、Numaga-Tomita Takuro、Kitajima Naoyuki、Toyama Takashi、Harada Eri、Shimauchi Tsukasa、Nishimura Akiyuki、Ishikawa Tatsuya、Kumagai Yoshito、Birnbaumer Lutz、Nishida Motohiro
    • Journal Title

      Sci Rep

      Volume: 7 Pages: 1-10

    • DOI

      10.1038/s41598-017-07903-4

    • NAID

      120006343876

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Cysteinyl-tRNA synthetase governs cysteine polysulfidation and mitochondrial bioenergetics.2017

    • Author(s)
      Akaike Takaaki、、、Nishida Motohiro(4)、、、、、、Fujii Shigemoto(20)、、、Motohashi Hozumi(26)
    • Journal Title

      Nature Commun

      Volume: 8 Pages: 1-10

    • DOI

      10.1038/s41467-017-01311-y

    • NAID

      120006365562

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Exposure to Electrophiles Impairs Reactive Persulfide-dependent Redox Signaling in Neuronal Cells2017

    • Author(s)
      Ihara Hideshi、Kasamatsu Shingo、Kitamura Atsushi、Nishimura Akira、Tsutsuki Hiroyasu、Ida Tomoaki、Ishizaki Kento、Toyama Takashi、Yoshida Eiko、Abdul Hamid Hisyam、Jung Minkyung、Matsunaga Tetsuro、Fujii Shigemoto、Sawa Tomohiro、Nishida Motohiro、Kumagai Yoshito、Akaike Takaaki
    • Journal Title

      Chemical Research Toxicology

      Volume: 30 Pages: 1673-1684

    • DOI

      10.1021/acs.chemrestox.7b00120

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The purinergic P2Y6 receptor heterodimerizes with the angiotensin AT1 receptor to promote angiotensin II-induced hypertension.2017

    • Author(s)
      A. Nishimura, C. Sunggip, H. Tozaki-Saitoh, T. Shimauchi, T. Numaga-Tomita, K. Hirano, T. Ide, J-M. Boeynaems, H. Kurose, M. Tsuda, B. Robaye, K. Inoue and M. Nishida
    • Journal Title

      Sci Signal.

      Volume: Jan 19;9(411)

    • DOI

      10.1126/scisignal.aac9187

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] MiR30‐GALNT1/2 Axis‐Mediated Glycosylation Contributes to the Increased Secretion of Inactive Human Prohormone for Brain Natriuretic Peptide (proBNP) From Failing Hearts2017

    • Author(s)
      Nakagawa Y, Nishikimi T, Kuwahara K, Fujishima A, Oka S, Tsutamoto T, Kinoshita H, Nakao K, Cho K, Inazumi H, Okamoto H, Nishida M, Kato T, Fukushima H, Yamashita JK, Wijnen WJ, Creemers EE, Kangawa K, Minamino N, Nakao K, Kimura T.
    • Journal Title

      J. Am. Heart Assoc.

      Volume: 6

    • DOI

      10.1161/jaha.116.003601

    • NAID

      120006360780

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Redox regulation of electrophilic signaling by reactive persulfides in cardiac cells.2017

    • Author(s)
      Nishida M, Nishimura A, Matsunaga T, Motohashi H, Kasamatsu S, Akaike T.
    • Journal Title

      Free radical biology & medicine

      Volume: 印刷中 Pages: 132-140

    • DOI

      10.1016/j.freeradbiomed.2017.01.024

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] プリン作動性シグナルの心血管系における役割2017

    • Author(s)
      西村明幸、西田基宏
    • Journal Title

      日本薬理学雑誌

      Volume: 149 Pages: 84-90

    • NAID

      130005306336

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Identification of C1q as a binding protein for advanced glycation end products2016

    • Author(s)
      Chikazawa, M., Shibata, T., Hatasa, Y., Hirose, S., Otaki, N., Nakashima, F., Ito, M., Machida, S., Maruyama, S., Uchida, K.
    • Journal Title

      Biochemistry

      Volume: 55 Pages: 435-446

    • DOI

      10.1021/acs.biochem.5b00777

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Oxidative deamination of serum albumins by (-)-epigallocatechin-3-O-gallate: A potential mechanism for the formation of innate antigens by antioxidants.2016

    • Author(s)
      Hatasa, Y., Chikazawa, M., Furuhashi, M., Nakashima, F., Shibata, T., Kondo, T., Akagawa, M., Hamagami, H., Tanaka, H., Tachibana, H., Uchida, K.
    • Journal Title

      PLoS ONE

      Volume: 11

    • DOI

      10.1371/journal.pone.0153002

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Identification of lactate dehydrogenase as a mammalian pyrroloquinoline quinone (PQQ)-binding protein.2016

    • Author(s)
      Akagawa, M., Minematsu, K., Shibata, T., Kondo, T., Ishii, T., and Uchida, K.
    • Journal Title

      Sci. Rep.

      Volume: 6 Pages: 26723-26723

    • DOI

      10.1038/srep26723

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Membrane lipid peroxidation in neurodegeneration: Role of thrombin and proteinase-activated receptor-12016

    • Author(s)
      Citron, B. A., Ameenuddin, S., Uchida, K., Suo, W. Z., SantaCruz, K., and Festoff, B. W.
    • Journal Title

      Brain Res.

      Volume: 1643 Pages: 10-17

    • DOI

      10.1016/j.brainres.2016.04.071

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Methylglyoxal, a glycolysis side-product, induces Hsp90 glycation and YAP-mediated tumor growth and metastasis2016

    • Author(s)
      Nokin, M. J., Durieux, F., Peixoto, P., Chiavarina, B., Peulen, O., Blomme, A., Turtoi, A., Costanza, B., Smargiasso, N., Baiwir, D., De Tullio, P., Bianchi, E., Thiry, M., Uchida, K., Spiegel, D. A., Hutton, C. A., De Pauw, E., Delvenne, P., Belpomme, D., Castronovo, D., and Bellahcène, A.
    • Journal Title

      eLife

      Volume: 5

    • DOI

      10.7554/elife.19375

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Screening of Transient Receptor Potential Canonical Channel Activators Identifies Novel Neurotrophic Piperazine Compounds.2016

    • Author(s)
      S. Sawamura, M. Hatano, Y. Takada, K. Hino, T. Kawamura, J. Tanikawa, H. Nakagawa, H. Hase, A. Nakao, M. Hirano, R. Rotrattanadumrong, S. Kiyonaka, M.X. Mori, M. Nishida, Y. Hu, R. Inoue, R. Nagata and Y. Mori
    • Journal Title

      Mol Pharmacol.

      Volume: 89(3) Pages: 348-363

    • DOI

      10.1124/mol.115.102863

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Sustained bAR stimulation mediates cardiac insulin resistance in a PKA-dependent manner2016

    • Author(s)
      S. Mangmool, T. Denkaew, S. Phosri, D. Pinthong, W. Parichatikanond, T. Shimauchi and M. Nishida
    • Journal Title

      Molecular Endocrinology

      Volume: 30(1) Pages: 118-132

    • DOI

      10.1210/me.2015-1201

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Recent progress in studies on the health benefits of pyrroloquinoline quinone2016

    • Author(s)
      Akagawa, M., Nakano, M., Ikemoto, K.
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 80 Pages: 13-22

    • DOI

      10.1080/09168451.2015.1062715

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis of radioiodinated probes to evaluate the biodistribution of a potent TRPC3 inhibitor.2016

    • Author(s)
      M. Hagimori, T. Murakami, K. Shimizu, M. Nishida, T. Ohshima, T. Mukai
    • Journal Title

      Medicinal Chemical Communications

      Volume: 7 Pages: 1003-1006

    • DOI

      10.1039/c6md00023a

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Methylmercury, an environmental electrophile capable of activation and disruption of the Akt/CREB/Bcl-2 signal transduction pathway in SH-SY5Y cells.2016

    • Author(s)
      Unoki T, Abiko Y, Toyama T, Uehara T, Tsuboi K, Nishida M, Kaji T, Kumagai Y.
    • Journal Title

      Scientific Reports

      Volume: 6 Pages: 1-10

    • DOI

      10.1038/srep28944

    • NAID

      120005822867

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] TRPC3 positively regulates reactive oxygen species driving maladaptive cardiac remodeling2016

    • Author(s)
      Kitajima N., Numaga-Tomita T., Watanabe M., Kuroda T., Nishimura A., Miyano K., Yasuda S., Kuwahara K., Sato Y., Ide T., Birnbaumer L., Sumimoto H., Mori Y. and Nishida M
    • Journal Title

      Sci Rep

      Volume: 6 Pages: 37001-37001

    • DOI

      10.1038/srep37001

    • NAID

      120006323744

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] TRPC3-GEF-H1 axis mediates pressure overload-induced cardiac fibrosis2016

    • Author(s)
      Numaga-Tomita T., Kitajima N., Kuroda T., Nishimura A., Miyano K., Yasuda S., Kuwahara K., Sato Y., Ide T., Birnbaumer L., Sumimoto H., Mori Y. and Nishida M.
    • Journal Title

      Sci Rep

      Volume: 6 Pages: 39383-39383

    • DOI

      10.1038/srep39383

    • NAID

      120005997825

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Redox signaling regulated by electrophiles and reactive sulfur species2016

    • Author(s)
      Nishida M, Kumagai Y, Ihara H, Fujii S, Motohashi H, Akaike T.
    • Journal Title

      J. Clin. Biochem. Nutr.

      Volume: 58 Issue: 2 Pages: 1-8

    • DOI

      10.3164/jcbn.15-111

    • NAID

      130005132519

    • ISSN
      0912-0009, 1880-5086
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] アダクトーム解析法を用いた疾患バイオマーカーの探索2016

    • Author(s)
      柴田貴広、内田浩二
    • Journal Title

      バイオサイエンスとインダストリー

      Volume: 74 Pages: 26-29

    • NAID

      40020705853

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Redox signaling regulated by an electrophilic cyclic nucleotide and reactive cysteine persulfides.2016

    • Author(s)
      Fujii S, Sawa T, Nishida M, Ihara H, Ida T, Motohashi H, Akaike T.
    • Journal Title

      Arch Biochem Biophys

      Volume: 595 Pages: 140-146

    • DOI

      10.1016/j.abb.2015.11.008

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] TRPC3 amplifies B-cell receptor-induced ERK signalling via protein kinase D-dependent Rap1 activation.2016

    • Author(s)
      T. Numaga-Tomita, M. Nishida, J.W. Jr. Putney and Y. Mori
    • Journal Title

      Biochem J.

      Volume: 473(2) Pages: 201-210

    • DOI

      10.1042/bj20150596

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Elastin modification by 4-hydroxynonenal in hairless mice exposed to UV-A. Role in photoaging and actinic elastosis2015

    • Author(s)
      Larroque-Cardoso, P., Camaré, C., Nadal-WollboldF., Grazide, M. H., Pucelle, M., Garoby-Salom, S., Bogdanowicz, P., Josse, G., Schmitt, A. M., Uchida, K., Zarkovic, K., Salvayre, R., and Nègre-Salvayre, A.
    • Journal Title

      J. Invest. Dermatol.

      Volume: 135 Pages: 1873-1881

    • DOI

      10.1038/jid.2015.84

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Abnormal myofiber morphology and limb dysfunction in claudication2015

    • Author(s)
      Koutakis, P., Myers, S. A., Cluff, K., Ha, D. M., Haynatzki, G., McComb, R. D., Uchida, K., Miserlis, D., Papoutsi, E., Johanning, J. M., Casale, G. P., and Pipinos, I. I.
    • Journal Title

      J. Surg. Res.

      Volume: J. Surg. Res. Pages: 172-179

    • DOI

      10.1016/j.jss.2015.02.011

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Generation of adducts of 4-hydroxy-2-nonenal with heat shock 60kDa protein 1 in human promyelocytic HL-60 and monocytic THP-1 cell lines2015

    • Author(s)
      Arcaro, A., Daga, M., Paolo Cetrangolo, G., Ciamporcero, E., Lepore, A., Pizzimenti, A., Petrella, C., Graf, M., Uchida, U., Mamone, G., Ferranti, P., Julian Ames, R., Palumbo, G., BarreraG., and Gentile, F.
    • Journal Title

      Oxid Med. Cell. Longev.

      Volume: 2015 Pages: 296146-296146

    • DOI

      10.1155/2015/296146

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Divergent Roles of CAAX Motif-signaled Posttranslational Modifications in the Regulation and Subcellular Localization of Ral GTPases.2015

    • Author(s)
      LR Gentry, A. Nishimura, A.D. Cox, T.D. Martin, D. Tsygankov, M. Nishida, T.C. Elston and C.J. Der
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 290(37) Pages: 22851-22861

    • DOI

      10.1074/jbc.m115.656710

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Reactive Sulfur Species-Mediated Activation of the Keap1-Nrf2 Pathway by 1,2-Naphthoquinone through Sulfenic Acids Formation under Oxidative Stress.2015

    • Author(s)
      Shinkai Y, Abiko Y, Ida T, Miura T, Kakehashi H, Ishii I, Nishida M, Sawa T, Akaike T, Kumagai Y.
    • Journal Title

      Chemical Research in Toxicology

      Volume: 印刷中 Pages: 838-847

    • DOI

      10.1021/tx500416y

    • NAID

      130005483607

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Measurement of glucose uptake in cultured cells2015

    • Author(s)
      Yamamoto, N., Ueda-Wakagi, M., Sato, T., Kawasaki, K., Sawada, K., Kawabata, K., Akagawa, M., Ashida, H.
    • Journal Title

      Curr. Protoc. Pharmacol.

      Volume: 71 Pages: 1-12

    • DOI

      10.1002/0471141755

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Production of hypoallergenic cocoa beans by a pregermination treatment method2015

    • Author(s)
      Handoyo, T., Akagawa, M., Kim, K.
    • Journal Title

      J. Plant Biotechnol.

      Volume: 42 Pages: 123-128

    • DOI

      10.5010/jpb.2015.42.2.123

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Aldehyde adducts generated during lipid peroxidation modification of proteins2015

    • Author(s)
      Uchida K
    • Journal Title

      Free Radic Res.

      Volume: 49 Pages: 896-904

    • DOI

      10.3109/10715762.2015.1036052

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Special issue on "Recent progress in lipid peroxidation based on novel approaches"2015

    • Author(s)
      Yin H, Niki E, Uchida K.
    • Journal Title

      Free Radic. Res.

      Volume: 49 Pages: 813-815

    • DOI

      10.3109/10715762.2015.1021697

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] 15-Deoxy-Δ12, 14-prostaglandin J2 as an electrophilic mediator2015

    • Author(s)
      Shibata T.
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 79 Pages: 1044-1049

    • DOI

      10.1080/09168451.2015.1012149

    • Related Report
      2015 Annual Research Report
    • Open Access
  • [Journal Article] 内因性抗原としての陰性荷電分子の生成と制御2015

    • Author(s)
      近澤未歩、内田浩二
    • Journal Title

      生化学

      Volume: 87 Pages: 776-780

    • NAID

      40020687514

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Palmitate induces insulin resistance in human HepG2 hepatocytes by enhancing ubiquitination and proteasomal degradation of key insulin signaling molecules2015

    • Author(s)
      Ishii M, Maeda A, Tani S, Akagawa M.
    • Journal Title

      Arch. Biochem. Biophys.

      Volume: 566 Pages: 26-35

    • DOI

      10.1016/j.abb.2014.12.009

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Toll-like receptors as a target of food-derived anti-Inflammatory compounds.2014

    • Author(s)
      Shibata, T., Nakashima, F., Honda, K., Lu, Y. J., Kondo, T., Ushida, Y., Aizawa, K., Suganuma, H., Oe, S., Tanaka, H., Takahashi, T. and Uchida, K.
    • Journal Title

      J. Biol. Chem.

      Volume: 289 Pages: 32757-32772

    • DOI

      10.1074/jbc.m114.585901

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Functional interaction between cyclooxygenase-2 and p53 in response to an endogenous electrophile2014

    • Author(s)
      Kumagai, T., Usami, H., Matsukawa, N., Nakashima, F., Chikazawa, M., Shibata, T., Noguchi, N., and Uchida, K.
    • Journal Title

      Redox Biol.

      Volume: 4 Pages: 74-86

    • DOI

      10.1016/j.redox.2014.11.011

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Inhibition of N-type Ca2+ channels ameliorates an imbalance in cardiac autonomic nerve activity and prevents lethal arrhythmias in mice with heart failure2014

    • Author(s)
      Yamada Y, Kinoshita H, Kuwahara K, Nakagawa Y, Kuwabara Y, Minami T, Yamada C, Shibata J, Nakao K, Cho K, Arai Y, Yasuno S, Nishikimi T, Ueshima K, Kamakura S, Nishida M, Kiyonaka S, Mori Y, Kimura T, Kangawa K, Nakao K.
    • Journal Title

      Cardiovascular Research

      Volume: 104 Pages: 183-193

    • DOI

      10.1093/cvr/cvu185

    • NAID

      120005619790

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Cardiovascular Aging Regulated by Heterodimerization of Angiotensin AT1 Receptor with Purinergic P2Y6 Receptor2018

    • Author(s)
      Nishida M.
    • Organizer
      Angiotensin Gordon Research Conference 2018
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] S-チオール化血清アルブミンによる炎症誘導機構の解明2018

    • Author(s)
      中島史恵、柴田貴広、菊地良介、松下正、内田浩二
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 自然抗体エピトープを有する食品成分の探索2018

    • Author(s)
      三輪恭行、今堀夏奈美、古橋麻衣、柴田貴広、内田浩二
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ポリフェノールオキシダーゼによる茶カテキンの自然抗体リガンド生成能の増強2018

    • Author(s)
      今堀夏奈美、三輪恭行、李相美、柴田貴広、内田浩二
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 酸化ストレスに対する細胞内カルノシンの神経保護効果2017

    • Author(s)
      山陰茜、垣花優希、柴田貴広、内田浩二、居原秀
    • Organizer
      第17回日本NO学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 酸化ストレスに依存した酸化イミダゾールジペプチドの産生2017

    • Author(s)
      垣花優希、柴田貴広、内田浩二、居原秀
    • Organizer
      第70回日本酸化ストレス学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Detection and quantification of 2-oxo-histidine-containing dipeptides2017

    • Author(s)
      Ihara H, Kakihana Y, Shibata T, Uchida K
    • Organizer
      The Society for Redox Biology and Medicine's (SfRBM) 24th Annual Meeting
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 脂質異常症に関連した修飾血清アルブミン構造の解析2017

    • Author(s)
      中島史恵、柴田貴広、菊地良介、松下正、内田浩二
    • Organizer
      2017年度生命科学系学会合同年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Protein carbonyls as a marker of exposome2017

    • Author(s)
      Koji Uchida
    • Organizer
      第 90 回日本細菌学会総会. レドックス・環境応答-国際シンポジウム(新学術領域「酸素生物学」共催) “Stress responses in environmental and exposure biology”
    • Place of Presentation
      仙台国際センター(宮城県仙台市)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Natural antibodies as a sensor of oxidation-specific epitopes2016

    • Author(s)
      Koji Uchida
    • Organizer
      Shanghai Symposium on Lipid Metabolism and Related Diseases
    • Place of Presentation
      Hope Hotel(Shanghai, China)
    • Year and Date
      2016-09-16
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Electrophiles as a multifunctional molecule2016

    • Author(s)
      Koji Uchida
    • Organizer
      第9回国際NO学会
    • Place of Presentation
      仙台国際センター(宮城県仙台市)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 親電子修飾によるタンパク質機能活性化2016

    • Author(s)
      内田浩二
    • Organizer
      第89回日本生化学会大会シンポジウム“オルガネラ環境を制御するレドックスシグナル”
    • Place of Presentation
      仙台国際センター(宮城県仙台市)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] リジンピロール化によるDNA様蛋白質の生成2015

    • Author(s)
      内田浩二
    • Organizer
      第15回日本蛋白質科学会年会ワークショップ「化学修飾の蛋白質科学と医療への展開」
    • Place of Presentation
      あわぎんホール(徳島県徳島市)
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 小分子酸素センサーによるタンパク質カルボニル化2015

    • Author(s)
      内田浩二
    • Organizer
      第15回日本蛋白質科学会年会ワークショップ「酸素リモデリング・レドックスシグナルとタンパク質修飾の新たな潮流」
    • Place of Presentation
      あわぎんホール(徳島県徳島市)
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] ポリフェノールによる自然免疫抗原の生成2015

    • Author(s)
      内田浩二
    • Organizer
      第9回日本ポリフェノール学会年次大会シンポジウム「ポリフェノール研究の将来」
    • Place of Presentation
      奈良女子大学(奈良県奈良市)
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 化学的反応性からみる機能性食品成分の潜在能力2015

    • Author(s)
      内田浩二
    • Organizer
      第38回日本分子生物学会・第88回日本生学会合同大会ワークショップ「食品科学:食品の潜在能力を科学し、活用する」
    • Place of Presentation
      神戸ポートピアホテル(兵庫県神戸市)
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Pyrrolation Is a Naturally-occurring Covalent Protein Modification Involved in the Production of DNA Mimic Proteins2014

    • Author(s)
      Koji Uchida
    • Organizer
      Asian Biological Medicine Summit Forum in Hangzhou
    • Place of Presentation
      Hangzhou, China
    • Year and Date
      2014-11-22 – 2014-11-25
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Food-derived natural inhibitors of TLR signaling2014

    • Author(s)
      Koji Uchida
    • Organizer
      HNE club/SAS meeting 2014 Lipids, Oxidation, Antioxidants and・・・・・
    • Place of Presentation
      Toulouse, France
    • Year and Date
      2014-09-08 – 2014-09-10
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 生体防御反応に関わる機能性食品成分2014

    • Author(s)
      内田浩二
    • Organizer
      2014年度 日本生物工学会中部支部例会
    • Place of Presentation
      名古屋大学工学研究科ES総合館(愛知県名古屋市)
    • Year and Date
      2014-08-01
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Book] Japanese Scientists in Science Signaling 2016「シグナリングに載った日本人研究者」 2017 Issue2017

    • Author(s)
      西村明幸、西田基宏
    • Total Pages
      80
    • Publisher
      Science Signaling AAAS, Cosmo Bio Co. Inc.
    • Related Report
      2016 Annual Research Report
  • [Book] Innovative Medicine2015

    • Author(s)
      Nishida M, Kuwahara K, Kozai D, Sakaguchi R, Mori Y.
    • Total Pages
      339
    • Publisher
      Springer Japan
    • Related Report
      2015 Annual Research Report
  • [Book] 別冊医学のあゆみ「レドックスUPDATE:ストレス制御の臨床医学・健康科学」2015

    • Author(s)
      内田浩二
    • Total Pages
      360
    • Publisher
      医歯薬出版
    • Related Report
      2015 Annual Research Report
  • [Book] 細胞工学 Vol.34 No.42015

    • Author(s)
      居原秀、大内雄也
    • Total Pages
      440
    • Publisher
      学研メディカル秀潤社
    • Related Report
      2014 Annual Research Report
  • [Book] 細胞工学 Vol.34 No.42014

    • Author(s)
      熊谷嘉人、内田浩二
    • Total Pages
      440
    • Publisher
      学研メディカル秀潤社
    • Related Report
      2014 Annual Research Report

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Published: 2014-11-20   Modified: 2018-12-17  

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