|Budget Amount *help
¥71,890,000 (Direct Cost: ¥55,300,000、Indirect Cost: ¥16,590,000)
Fiscal Year 2018: ¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2017: ¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2016: ¥12,220,000 (Direct Cost: ¥9,400,000、Indirect Cost: ¥2,820,000)
Fiscal Year 2015: ¥12,220,000 (Direct Cost: ¥9,400,000、Indirect Cost: ¥2,820,000)
Fiscal Year 2014: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000)
|Outline of Final Research Achievements
We generated transgenic songbirds to manipulate the transcription factor CREB , which controls gene expression in an activity dependent manner. We compared song similarity to tutor birds' songs between CREB mutants and wild type. We found that the dominant negative CREB mutant transgenic birds developed songs with reduced similarity in comparison with wild type birds. In contrast, no significant difference was observed in calls between wild types and CREB transgenics. These results indicate that CREB activation is essential for the vocal learning (Abe et al., PNAS 2015).
To study the relationship between neural activity and activity-dependent intracellular signaling, we devised a novel endomicroscopy. Using this endomicroscopy, we successfully measured the neural activity and ERK activity at a single-cell resolution in a freely behaving animal.