Chemical taxonomy of ncRNA

• Project Area: Neo-taxonomy of noncoding RNAs
• Project/Area Number: 26113003
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo
• Principal Investigator: Suzuki Tsutomu 東京大学, 大学院工学系研究科(工学部), 教授 (20292782)
• Project Period (FY): 2014-07-10 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥133,900,000 (Direct Cost: ¥103,000,000、Indirect Cost: ¥30,900,000); Fiscal Year 2018: ¥26,520,000 (Direct Cost: ¥20,400,000、Indirect Cost: ¥6,120,000); Fiscal Year 2017: ¥26,520,000 (Direct Cost: ¥20,400,000、Indirect Cost: ¥6,120,000); Fiscal Year 2016: ¥26,520,000 (Direct Cost: ¥20,400,000、Indirect Cost: ¥6,120,000); Fiscal Year 2015: ¥26,520,000 (Direct Cost: ¥20,400,000、Indirect Cost: ¥6,120,000); Fiscal Year 2014: ¥27,820,000 (Direct Cost: ¥21,400,000、Indirect Cost: ¥6,420,000)
• Keywords: RNA修飾 / マススペクトロメトリー / mRNA / ｔRNA / rRNA / リボソーム / エピトランスクリプトミクス / タンパク質合成 / tRNA修飾 / pre-tRNA capping / f5C修飾

Outline of Final Research Achievements

This project aimed to identify novel RNA modifications as functional elements of RNA molecules. We identified a cap-specific novel m6A writer for mRNA, and an RNA acetyltransferase using an acetate ion as a substrate. We studied CO2-sensitive tRNA modification and its association with Warburg effect, and found dynamic regulation of tRNA modification by sensing cellular metabolic status. In addition, we reported pre-tRNA capping, several novel RNA modifications and growth phase-dependent alteration of tRNA modification. We elucidated biogenesis of 5-formylcytidine in human mitochondrial tRNA.

Academic Significance and Societal Importance of the Research Achievements

本研究において、私たちは新規RNA修飾や修飾酵素を多数発見した。これらの成果は、RNA修飾が関与する生命現象の理解に貢献するだけでなく、RNA修飾が欠損することで生じるヒトの疾患（RNA修飾病）の発症原因の解明および具体的な治療法や創薬のための基盤的知見を提供する。

Research Products

• [Int'l Joint Research] IMB Mainz/Friedrich-Wilhelms-Universität Bonn(ドイツ)
• [Int'l Joint Research] 浙江大学(中国)
• [Int'l Joint Research] Thomas Jefferson Univ(米国)
• [Int'l Joint Research] Lomonosov Moscow State University(ロシア連邦)
• [Int'l Joint Research] Australian National University(オーストラリア)
• [Int'l Joint Research]
• [Int'l Joint Research] IMB Mainz/Friedrich-Wilhelms-Universität Bonn(ドイツ)
• [Int'l Joint Research] 浙江大学(中国)
• [Int'l Joint Research] Thomas Jefferson Univ(米国)
• [Int'l Joint Research] Lomonosov Moscow State University(ロシア連邦)
• [Int'l Joint Research] Australian National University(オーストラリア)
• [Int'l Joint Research]
• [Int'l Joint Research] Lodz University of Technology(ポーランド)
• [Int'l Joint Research] Ohio State University(米国)
• [Int'l Joint Research] 浙江大学(中国)
• [Int'l Joint Research] Queen's University(カナダ)
• [Int'l Joint Research] Ben-Gurion University of the Negev(イスラエル)
• [Int'l Joint Research]
• [Journal Article] Impact of intron removal from tRNA genes on Saccharomyces cerevisiae (2019)
  • Author(s): Hayashi Sachiko、Mori Shunsuke、Suzuki Takeo、Suzuki Tsutomu、Yoshihisa Tohru
  • Journal Title: Nucleic Acids Research Volume: - Issue: 11 Pages: 5936-5949
  • DOI: 10.1093/nar/gkz270
  • Peer Reviewed / Open Access
• [Journal Article] Depletion of S-adenosylmethionine impacts on ribosome biogenesis through hypomodification of a single rRNA methylation (2019)
  • Author(s): Ishiguro Kensuke、Arai Taiga、Suzuki Tsutomu
  • Journal Title: Nucleic Acids Research Volume: 47 Issue: 8 Pages: 4226-4239
  • DOI: 10.1093/nar/gkz111
  • Peer Reviewed / Open Access
• [Journal Article] Random mutagenesis of a hyperthermophilic archaeon identified tRNA modifications associated with cellular hyperthermotolerance (2019)
  • Author(s): Orita Izumi、Futatsuishi Ryohei、Adachi Kyoko、Ohira Takayuki、Kaneko Akira、Minowa Keiichi、Suzuki Miho、Tamura Takeshi、Nakamura Satoshi、Imanaka Tadayuki、Suzuki Tsutomu、Fukui Toshiaki
  • Journal Title: Nucleic Acids Research Volume: 47 Issue: 4 Pages: 1964-1976
  • DOI: 10.1093/nar/gky1313
  • Peer Reviewed / Open Access
• [Journal Article] Transcriptome-wide identification of A-to-I RNA editing sites using ICE-seq (2019)
  • Author(s): Okada Shunpei、Ueda Hiroki、Noda Yuta、Suzuki Tsutomu
  • Journal Title: Methods Volume: 156 Pages: 66-78
  • DOI: 10.1016/j.ymeth.2018.12.007
  • Peer Reviewed / Open Access
• [Journal Article] Cap-specific terminal N6-methylation of RNA by an RNA polymerase II?associated methyltransferase (2018)
  • Author(s): Akichika Shinichiro、Hirano Seiichi、Shichino Yuichi、Suzuki Takeo、Nishimasu Hiroshi、Ishitani Ryuichiro、Sugita Ai、Hirose Yutaka、Iwasaki Shintaro、Nureki Osamu、Suzuki Tsutomu
  • Journal Title: Science Volume: 363 Issue: 363 Pages: 1-7
  • DOI: 10.1126/science.aav0080
  • Peer Reviewed / Open Access
• [Journal Article] Quantification of methylation efficiency at a specific N6-methyladenosine position in rRNA by using BNA probes (2018)
  • Author(s): Oshima Takuya、Ishiguro Kensuke、Suzuki Tsutomu、Kawahara Yukio
  • Journal Title: Chemical Communications Volume: 54 Issue: 69 Pages: 9627-9630
  • DOI: 10.1039/c8cc03713b
  • Peer Reviewed
• [Journal Article] Acetate-dependent tRNA acetylation required for decoding fidelity in protein synthesis (2018)
  • Author(s): Taniguchi T, Miyauchi K, Sakaguchi Y, Yamashita S, Soma A, Tomita K, Suzuki T
  • Journal Title: Nature Chemical Biology Volume: 14 Issue: 11 Pages: 1010-1020
  • DOI: 10.1038/s41589-018-0119-z
  • Peer Reviewed
• [Journal Article] CO2-sensitive tRNA modification associated with human mitochondrial disease (2018)
  • Author(s): Lin, H., Kenjyo, M., Harada, T., Okita, R., Takeshita, E., Komaki, H., Yagasaki, H., Fujioka, K., Goto, Y., Yanaka, K., Nakagawa, S., Sakaguchi, Y. and *Suzuki, T.
  • Journal Title: Nature Commun. Volume: in press Issue: 1 Pages: 1875-1875
  • DOI: 10.1038/s41467-018-04250-4
  • Peer Reviewed / Open Access
• [Journal Article] Accurate estimation of 5-methylcytosine in mammalian mitochondrial DNA (2018)
  • Author(s): Matsuda, S., *Yasukawa, T., Sakaguchi, Y., Ichiyanagi, K., Unoki, M., Gotoh, K., Fukuda, K., Sasaki, H., Suzuki, T. and Kang, D.
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 5801-5801
  • DOI: 10.1038/s41598-018-24251-z
  • Peer Reviewed / Open Access
• [Journal Article] The ribosomal A-site finger is crucial for binding and activation of the stringent factor RelA (2018)
  • Author(s): Kudrin, P., Dzhygyr, I., Ishiguro, K., Beljantseva, J., Maksimova, E., Sofia Raquel Alves Oliveira, Varik, V., Payoe, R., Konevega, A., Tenson, T., Suzuki, T. and Hauryliuk, V.
  • Journal Title: Nucleic Acids Res. Volume: 46 Issue: 4 Pages: 1973-1983
  • DOI: 10.1093/nar/gky023
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Metabolic and chemical regulation of tRNA modification associated with taurine deficiency and human disease (2018)
  • Author(s): Asano Kana、Suzuki Takeo、Saito Ayaka、Wei Fan-Yan、Ikeuchi Yoshiho、Numata Tomoyuki、Tanaka Ryou、Yamane Yoshihisa、Yamamoto Takeshi、Goto Takanobu、Kishita Yoshihito、Murayama Kei、Ohtake Akira、Okazaki Yasushi、Tomizawa Kazuhito、Sakaguchi Yuriko、Suzuki Tsutomu
  • Journal Title: Nucleic Acids Res. Volume: 46 Issue: 4 Pages: 1565-1583
  • DOI: 10.1093/nar/gky068
  • Peer Reviewed / Open Access
• [Journal Article] Defective Mitochondrial tRNA Taurine Modification Activates Global Proteostress and Leads to Mitochondrial Disease. (2018)
  • Author(s): Fakruddin M, Wei FY, Suzuki T, Asano K, Kaieda T, Omori A, Izumi R, Fujimura A, Kaitsuka T, Miyata K, Araki K, Oike Y, Scorrano L, Suzuki T, Tomizawa K.
  • Journal Title: Cell Rep. Volume: 22 Issue: 2 Pages: 482-496
  • DOI: 10.1016/j.celrep.2017.12.051
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] ALKBH1 is an RNA dioxygenase responsible for cytoplasmic and mitochondrial tRNA modifications (2017)
  • Author(s): Kawarada Layla、Suzuki Takeo、Ohira Takayuki、Hirata Shoji、Miyauchi Kenjyo、Suzuki Tsutomu
  • Journal Title: Nucleic Acids Res. Volume: 45 Issue: 12 Pages: 7401-7415
  • DOI: 10.1093/nar/gkx354
  • Peer Reviewed / Open Access
• [Journal Article] Biochemical and structural characterization of oxygen-sensitive 2-thiouridine synthesis catalyzed by an iron-sulfur protein TtuA (2017)
  • Author(s): Chen Minghao、Asai Shin-ichi、Narai Shun、Nambu Shusuke、Omura Naoki、Sakaguchi Yuriko、Suzuki Tsutomu、Ikeda-Saito Masao、Watanabe Kimitsuna、Yao Min、Shigi Naoki、Tanaka Yoshikazu
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 114 Issue: 19 Pages: 4954-4959
  • DOI: 10.1073/pnas.1615585114
  • Peer Reviewed / Open Access
• [Journal Article] Human BCDIN3D Monomethylates Cytoplasmic Histidine Transfer RNA. (2017)
  • Author(s): Martinez A, Yamashita S, Nagaike T, Sakaguchi Y, Suzuki T, Tomita K
  • Journal Title: Nucleic Acids Res Volume: - Pages: 5423-5436
  • DOI: 10.1093/nar/gkx051
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] RNA editing enzyme ADAR2 is a mediator of neuropathic pain after peripheral nerve injury. (2017)
  • Author(s): Uchida H, Matsumura S, Okada S, Suzuki T, Minami T and Ito S
  • Journal Title: FASEB Journal Volume: 印刷中 Issue: 5 Pages: 1847-1855
  • DOI: 10.1096/fj.201600950r
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] S -Adenosylmethionine Synthesis Is Regulated by Selective N 6 -Adenosine Methylation and mRNA Degradation Involving METTL16 and YTHDC1 (2017)
  • Author(s): Shima Hiroki、Matsumoto Mitsuyo、Ishigami Yuma、Ebina Masayuki、Muto Akihiko、Sato Yuho、Kumagai Sayaka、Ochiai Kyoko、Suzuki Tsutomu、Igarashi Kazuhiko
  • Journal Title: Cell Rep. Volume: 21 Issue: 12 Pages: 3354-3363
  • DOI: 10.1016/j.celrep.2017.11.092
  • Peer Reviewed / Open Access
• [Journal Article] Biogenesis and iron-dependency of ribosomal RNA hydroxylation (2017)
  • Author(s): Kimura, S., Sakai, Y., Ishiguro, K. and Suzuki, T.
  • Journal Title: Nucleic Acids Res. Volume: 45 Issue: 22 Pages: 12974-12986
  • DOI: 10.1093/nar/gkx969
  • Peer Reviewed / Open Access
• [Journal Article] Intrinsic Ribosome Destabilization Underlies Translation and Provides an Organism with a Strategy of Environmental Sensing (2017)
  • Author(s): Chadani Yuhei、Niwa Tatsuya、Izumi Takashi、Sugata Nobuyuki、Nagao Asuteka、Suzuki Tsutomu、Chiba Shinobu、Ito Koreaki、Taguchi Hideki
  • Journal Title: Molecular Cell Volume: 68 Issue: 3 Pages: 528-539
  • DOI: 10.1016/j.molcel.2017.10.020
  • Peer Reviewed
• [Journal Article] Hydroxylation of a conserved tRNA modification establishes non-universal genetic code in echinoderm mitochondria (2017)
  • Author(s): Nagao, A., Ohara, M., Miyauchi, K., Yokobori, S., Yamagishi, A., Watanabe, K. and *Suzuki, T.
  • Journal Title: Nature Structural & Molecular Biology Volume: 24 Issue: 9 Pages: 778-782
  • DOI: 10.1038/nsmb.3449
  • Peer Reviewed
• [Journal Article] A hydantoin isoform of cyclic N6-threonylcarbamoyladenosine (ct6A) is present in tRNAs (2017)
  • Author(s): Matuszewski, M., Wojciechowski, J., Miyauchi, K., Gdaniec, Z., Wolf, W. M., Suzuki, T. and Sochacka, E
  • Journal Title: Nucleic Acids Res Volume: 45 Issue: 4 Pages: 2137-2149
  • DOI: 10.1093/nar/gkw1189
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification of 2-methylthio cyclic N6-threonylcarbamoyladenosine (ms2ct6A) as a novel RNA modification at position 37 of tRNAs (2017)
  • Author(s): Kang, B. I., Miyauchi, K., Matuszewski, M., D'Almeida, G. S., Rubio, M. A., Alfonzo, J. D., Inoue, K., Sakaguchi, Y., Suzuki, T., Sochacka, E., Suzuki, T.
  • Journal Title: Nucleic Acids Res Volume: 45 Issue: 4 Pages: 2124-2136
  • DOI: 10.1093/nar/gkw1120
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structural and functional characterization of the TYW3/Taw3 class of SAM-dependent methyltransferases (2017)
  • Author(s): Currie, M. A., Brown, G., Wong, A., Ohira, T., Sugiyama, K., Suzuki, T.,Yakunin, A. F., and Jia, Z
  • Journal Title: RNA Volume: 23 Issue: 3 Pages: 346-354
  • DOI: 10.1261/rna.057943.116
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Duplication of Drosophila melanogaster mitochondrial EF-Tu: pre-adaptation to T-arm truncation and exclusion of bulky aminoacyl residues (2017)
  • Author(s): Sato, A., Suematsu, T., Aihara, K., Kita, K., Suzuki, T., Watanabe, K., Ohtsuki, T. and Watanabe, Y.
  • Journal Title: Biochem. J. Volume: 474 Issue: 6 Pages: 957-969
  • DOI: 10.1042/bcj20160929
  • Peer Reviewed
• [Journal Article] RNA modifications: what have we learned and where are we headed? (2016)
  • Author(s): Frye, M., Jaffrey, S., Pan, T., Rechavi, G. and Suzuki, T.
  • Journal Title: Nature Rev Genet Volume: 17 Issue: 6 Pages: 365-372
  • DOI: 10.1038/nrg.2016.47
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] NSUN3 methylase initiates 5-formylcytidine biogenesis in human mitochondrial tRNA(Met) (2016)
  • Author(s): Nakano, S., Suzuki, T., Kawarada, L., Iwata, H., Asano, K., Suzuki, T.
  • Journal Title: Nat Chem Biol Volume: 12 Issue: 7 Pages: 546-51
  • DOI: 10.1038/nchembio.2099
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Precursors of tRNAs are stabilized by methylguanosine cap structures. (2016)
  • Author(s): Ohira T, Suzuki T
  • Journal Title: Nat Chem Biol. Volume: 12 Issue: 8 Pages: 648-55
  • DOI: 10.1038/nchembio.2117
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Mitochondrial 16S rRNA Is Methylated by tRNA Methyltransferase TRMT61B in All Vertebrates (2016)
  • Author(s): Bar-Yaacov D, Frumkin I, Yashiro Y, Chujo T, Ishigami Y, Chemla Y, Blumberg A, Schlesinger O, Bieri P, Greber B, Ban N, Zarivach R, Alfonta L, Pilpel Y, Suzuki T, Mishmar D.
  • Journal Title: PLoS Biology Volume: - Issue: 9 Pages: e1002557-e1002557
  • DOI: 10.1371/journal.pbio.1002557
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Mtu1-mediated thiouridine formation of mitochondrial tRNAs is required for mitochondrial translation and is involved in reversible infantile liver injury (2016)
  • Author(s): Wu, Y., Wei, F. Y., Kawarada, L., Suzuki, T., Araki, K., Komohara, Y., Fujimura, A., Kaitsuka, T., Takeya, M., Oike, Y., Suzuki, T., Tomizawa, K.
  • Journal Title: PLoS genetics Volume: 12 Issue: 9 Pages: e1006355-e1006355
  • DOI: 10.1371/journal.pgen.1006355
  • Peer Reviewed / Open Access
• [Journal Article] A comprehensive genomic analysis reveals the genetic landscape of mitochondrial respiratory chain deficiency. (2016)
  • Author(s): Kohda M, Mizuno Y, Hirata T, Yatsuka Y, Yamashita, Okuda A, Borna NN, Banshoya K, Ohnuma K, Suzuki T, Nagao A, Maehata H, Matsuda F, Higasa K, Nagasaki M, Yasuda J, Yamamoto M, Fushimi T, Shimura M, Kaiho-Ichimoto K, Harashima H, Yamazaki T, Mori M, Murayama K, Ohtake A, Okazaki Y.
  • Journal Title: PLoS Genetics: Volume: 12(1) Issue: 1 Pages: e1005679-e1005679
  • DOI: 10.1371/journal.pgen.1005679
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Biogenesis and growth phase-dependent alteration of 5-methoxycarbonylmethoxyuridine in tRNA anticodons (2016)
  • Author(s): Yusuke Sakai, Kenjyo Miyauchi, Satoshi Kimura, Tsutomu Suzuki
  • Journal Title: Nucleic Acids Research Volume: 44 Issue: 2 Pages: 509-523
  • DOI: 10.1093/nar/gkv1470
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Identification and Functional Analysis of the Pre-piRNA 3' Trimmer in Silkworms (2016)
  • Author(s): Izumi, N., Shoji, K., Sakaguchi, Y., Honda, S., Kirino, Y., Suzuki, T., Katsuma, S. and Tomari, Y.
  • Journal Title: Cell Volume: 164 Pages: 962-973
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Iron-sulfur proteins responsible for RNA modifications (2015)
  • Author(s): Kimura S., Suzuki T.
  • Journal Title: Biochimica et Biophysica Acta Volume: 1853 Issue: 6 Pages: 1272-1283
  • DOI: 10.1016/j.bbamcr.2014.12.010
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Defining fundamental steps in the assembly of Drosophila RNAi enzyme complex (2015)
  • Author(s): Shintaro Iwasaki, Hiroshi M. Sasaki, Yuriko Sakaguchi, Tsutomu Suzuki, Hisashi Tadakuma, & Yukihide Tomari
  • Journal Title: Nature Volume: NA Issue: 7553 Pages: 0-0
  • DOI: 10.1038/nature14254
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Transcriptome-wide identification of adenosine-to-inosine editing using the ICE-seq method (2015)
  • Author(s): Suzuki, T., Ueda, H., Okada, S. and Sakurai, M.
  • Journal Title: Nature Protocol Volume: 10 Issue: 5 Pages: 715-732
  • DOI: 10.1038/nprot.2015.037
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Biochemical and transcriptome-wide identification of A-to-I RNA editing sites by ICE-seq (2015)
  • Author(s): Okada, S., Sakurai, M., Ueda, H. and Suzuki, T.
  • Journal Title: Methods in Enzymol Volume: 560 Pages: 331-353
  • DOI: 10.1016/bs.mie.2015.03.014
  • ISBN: 9780128021927
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Nucleoside analysis by hydrophilic interaction liquid chromatography coupled with mass spectrometry (2015)
  • Author(s): Sakaguchi, Y., Miyauchi, K., Kang, B. and Suzuki, T.
  • Journal Title: Methods in Enzymol Volume: 560 Pages: 19-28
  • DOI: 10.1016/bs.mie.2015.03.015
  • ISBN: 9780128021927
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Ribosomal RNA methyltransferases contribute to Staphylococcus aureus virulence (2015)
  • Author(s): Kyuma T, Kimura S, Hanada Y, Suzuki T, Sekimizu K, Kaito C
  • Journal Title: FEBS J. Volume: 282 Issue: 13 Pages: 2570
  • DOI: 10.1111/febs.13302
  • URL: https://pure.teikyo.jp/en/publications/fc32efa5-06e5-4013-80a5-f23105024de8
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] RlmCD-mediated U747 methylation promotes efficient G748 methylation by methyltransferase RlmAⅡ in 23S rRNA in Streptococcus pneumoniae; interplay between two rRNA methylations responsible for telithromycin susceptibility. (2015)
  • Author(s): Shoji T, Takaya A, Sato Y, Kimura S, Suzuki T, Yamamoto T
  • Journal Title: Nucleic Acids Research Volume: 43 Issue: 18 Pages: 8964-72
  • DOI: 10.1093/nar/gkv609
  • Peer Reviewed / Open Access
• [Journal Article] Destabilization of microRNA-122 in human cells by 3’ deadenylation mediated by PARN and CUGBP1 (2015)
  • Author(s): Katoh, T., Hojo, H. and Suzuki, T.
  • Journal Title: Nucleic Acids Research Volume: 43 Issue: 15 Pages: 7521-7534
  • DOI: 10.1093/nar/gkv669
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A single methylation of 23S rRNA triggers late steps of 50S ribosomal subunit assembly (2015)
  • Author(s): Arai, T., Ishiguro, K., Kimura, S., Sakaguchi, Y., Suzuki, T. and Suzuki, T.
  • Journal Title: Proc. Natl. Acad. Sci. USA. Volume: 112 Issue: 34
  • DOI: 10.1073/pnas.1506749112
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Regulation of gene expression via retrotransposon insertions and the noncoding RNA 4.5S RNAH. (2015)
  • Author(s): Ishida K, Miyauchi K, Kimura Y, Mito M, Okada S, Suzuki T, Nakagawa S.
  • Journal Title: Genes Cells Volume: 20 Issue: 11 Pages: 887-901
  • DOI: 10.1111/gtc.12280
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Rectifier of aberrant mRNA splicing recovers tRNA modification in familial dysautonomia. (2015)
  • Author(s): Yoshida M, Kataoka N, Miyauchi K, Ohe K, Iida K, Yoshida S, Nojima T, Okuno Y, Onogi H, Usui T, Takeuchi A, Hosoya T, Suzuki T, Hagiwara M.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 112（9） Issue: 9 Pages: 2764-2769
  • DOI: 10.1073/pnas.1415525112
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Cdk5rap1-mediated 2-methylthio modification of mitochondrial tRNAs governs protein translation and contributes to myopathy in mice and humans. (2015)
  • Author(s): Wei FY, Zhou B, Suzuki T, Miyata K, Ujihara Y, Horiguchi H, Takahashi N, Xie P, Michiue H, Fujimura A, Kaitsuka T, Matsui H, Koga Y, Mohri S, Suzuki T, Oike Y, Tomizawa K.
  • Journal Title: Cell Metab Volume: 21 Issue: 3 Pages: 413-6
  • DOI: 10.1016/j.cmet.2015.01.019
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A complete landscape of post-transcriptional modifications in mammalian mitochondrial tRNAs (2014)
  • Author(s): Suzuki T. and Suzuki T.
  • Journal Title: Nucleic Acids Research Volume: (in press) Issue: 11 Pages: 7346-7357
  • DOI: 10.1093/nar/gku390
  • Peer Reviewed / Open Access
• [Journal Article] Discovery of the b-barrel-type RNA methyltransferase responsible for N6-methylation of N6-threonylcarbamoyladenosine in tRNAs (2014)
  • Author(s): Kimura S., Miyauchi K., Ikeuchi Y., Thiaville PC., Crecy-Lagard Vd., Suzuki T.
  • Journal Title: Nucleic Acids Research Volume: 42 Issue: 14 Pages: 9350-9365
  • DOI: 10.1093/nar/gku618
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A single acetylation of 18S rRNA is essential for biogenesis of the small ribosomal subunit in Saccharomyces cerevisiae (2014)
  • Author(s): Ito, S., Akamatsu, Y., Noma, A., Kimura, S., Miyauchi, K., Ikeuchi, Y., Suzuki, T. and Suzuki, T
  • Journal Title: J. Biol. Chem. Volume: 289 Issue: 38 Pages: 26201-26212
  • DOI: 10.1074/jbc.m114.593996
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Human NAT10 is an ATP-dependent RNA acetyltransferase responsible for N4-acetylcytidine formation in 18S rRNA (2014)
  • Author(s): Satoshi Ito, Sayuri Horikawa, Tateki Suzuki, Hiroki Kawauchi, Yoshikazu Tanaka, Takeo Suzuki, and Tsutomu Suzuki
  • Journal Title: The Journal of Biological Chemistry Volume: 289 Issue: 52 Pages: 35724-35730
  • DOI: 10.1074/jbc.c114.602698
  • Peer Reviewed / Open Access
• [Journal Article] 5-Hydroxymethylcytosine plays a critical role in glioblastomagenesis by recruiting the CHTOP-Methylosome complex (2014)
  • Author(s): Hiroki Takai, Tetsu Akiyama et al.
  • Journal Title: Cell Reports Volume: 9 Issue: 1 Pages: 48
  • DOI: 10.1016/j.celrep.2014.08.071
  • URL: https://localhost/en/publications/faa4b7c1-5309-40ea-a2ad-a9e0a109b0d2
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Convergent evolution of AUA decoding in bacteria and archaea (2014)
  • Author(s): Tsutomu Suzuki and Tomoyuki Numata
  • Journal Title: RNA Biology Volume: 11 Issue: 12 Pages: 1586-1596
  • DOI: 10.4161/15476286.2014.992281
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Quality control of protein synthesis by peptidyl-tRNA drop off in the early elongation stage (2019)
  • Author(s): Tsutomu Suzuki
  • Organizer: Ribosome Structure and Function 2019
  • Int'l Joint Research / Invited
• [Presentation] RNA modification, a chemical diversity for biological function (2019)
  • Author(s): Tsutomu Suzuki
  • Organizer: The 20th Takeda Science Foundation Symposium on Bioscience“RNA Neobiology”
  • Int'l Joint Research / Invited
• [Presentation] Dynamic regulation of RNA modification in health and disease (2019)
  • Author(s): Tsutomu Suzuki
  • Organizer: Translation Machinery in Health and Disease (Gordon Research Conference)
  • Int'l Joint Research / Invited
• [Presentation] Metabolic and Chemical Regulation of RNA Modifications Associated with Various Biological Processes (2019)
  • Author(s): Tsutomu Suzuki
  • Organizer: RNA Editing, Next-Generation Epitranscriptomics in Health and Disease (Gordon Research Conference)
  • Int'l Joint Research / Invited
• [Presentation] Metabolic regulation of tRNA modifications associated with mitochondrial diseases (2018)
  • Author(s): Tsutomu Suzuki
  • Organizer: EMBO workshop on "Molecular Biology of Mitochondrial Gene Expression" in 2018
  • Int'l Joint Research / Invited
• [Presentation] RNA修飾によるエピトランスクリプトーム制御と疾患 (2018)
  • Author(s): 鈴木 勉
  • Organizer: 日本筋学会第4回学術集会
  • Invited
• [Presentation] Dynamic regulation of tRNA modification under physiological and pathological conditions (2018)
  • Author(s): Tsutomu Suzuki
  • Organizer: 27th tRNA conference
  • Int'l Joint Research / Invited
• [Presentation] Metabolic regulation of RNA modification associated with human disease (2018)
  • Author(s): 鈴木 勉
  • Organizer: 熊大発生医学研HIGOセミナー
  • Invited
• [Presentation] RNA modification, a chemical diversity for biological function (2018)
  • Author(s): Tsutomu Suzuki
  • Organizer: The 2nd Joint Australia-Japan joint RNA meeting 2018
  • Int'l Joint Research / Invited
• [Presentation] Molecular pathogenesis of mitochondrial disease caused by tRNA modification deficiency (2017)
  • Author(s): Tsutomu Suzuki
  • Organizer: FASEB science research conferences-Mitochondrial Biogenesis and Dynamics in Health, Disease and Aging
  • Int'l Joint Research / Invited
• [Presentation] Molecular pathogenesis of human disease caused by tRNA modification deficiency (2017)
  • Author(s): Tsutomu Suzuki
  • Organizer: The 43rd Naito Conference- Noncoding RNA：Biology, Chemistry, & Diseases
  • Int'l Joint Research / Invited
• [Presentation] RNA修飾によるエピトランスクリプトーム制御と疾患 (2017)
  • Author(s): 鈴木 勉
  • Organizer: 千里ライフサイエンスセミナー: 生命を司り、操る～ノンコーディングRNAの底知れぬ力
  • Invited
• [Presentation] Metabolic regulation of tRNA modification associated with human disease (2017)
  • Author(s): Tsutomu Suzuki
  • Organizer: IMB symposium on Nucleic Acid Modification
  • Int'l Joint Research / Invited
• [Presentation] 細胞内メタボライトを感知するRNA修飾と疾患 (2017)
  • Author(s): 鈴木 勉
  • Organizer: IIBMP2017シンポジウム「ポストゲノム時代のRNAバイオロジー」
  • Invited
• [Presentation] "Metabolic regulation of RNA modification associated with human disease " (2017)
  • Author(s): Tsutomu Suzuki
  • Organizer: Cold Spring Harbor Asia "RNA modification and epitranscriptome"
  • Int'l Joint Research / Invited
• [Presentation] Metabolic regulation of RNA modification associated with human disease (2017)
  • Author(s): Tsutomu Suzuki
  • Organizer: ConBio2017 "Emerging world of RNA modifications and epitranscriptome"
  • Int'l Joint Research / Invited
• [Presentation] RNA mass spectrometry: a platform technology for emerging world of RNA modifications and epitranscriptome (2017)
  • Author(s): Tsutomu Suzuki
  • Organizer: 第1回京都生体質量分析研究会シンポジウム
  • Int'l Joint Research / Invited
• [Presentation] RNA修飾によるエピトランスクリプトーム制御と疾患 (2017)
  • Author(s): 鈴木 勉
  • Organizer: 富山RNAワークショップ（富山大生命融合科学教育部セミナー）
  • Invited
• [Presentation] Pre-tRNA capping in yeast and mammals (2016)
  • Author(s): Tsutomu Suzuki
  • Organizer: IBS-CNRS Joint RNA Symposium
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2016-03-21
  • Invited
• [Presentation] RNA修飾の多彩な機能と疾患 (2016)
  • Author(s): 鈴木 勉
  • Organizer: 国際高等研究所プロジェクト「生命活動を生体高分子への修飾から俯瞰する」平成27年度研究会
  • Place of Presentation: 国際高等研究所、京都府木津川市
  • Year and Date: 2016-02-09
  • Invited
• [Presentation] RNAから病気の原因を探る (2016)
  • Author(s): 鈴木 勉
  • Organizer: 東京大学化学生命工学専攻公開講座「化学生命工学―未来への挑戦－」
  • Place of Presentation: 東京大学本郷キャンパス、東京都文京区
  • Year and Date: 2016-01-23
  • Invited
• [Presentation] Molecular pathogenesis of human mitochondrial diseases associated with decoding disorder (2016)
  • Author(s): Tsutomu Suzuki
  • Organizer: RNA2016 satellite-symposium 'Nascent biology and Ribosome functions'
  • Int'l Joint Research / Invited
• [Presentation] Insights into molecular pathogenesis of mitochondrial diseases caused by tRNA modification deficiency (2016)
  • Author(s): Tsutomu Suzuki
  • Organizer: 26th tRNA conference
  • Int'l Joint Research / Invited
• [Presentation] Biogenesis and physiological role of 5-formylcytidine in human mitochondrial tRNA (2016)
  • Author(s): Tsutomu Suzuki
  • Organizer: RNA modifications and epitranscriptomics conference
  • Int'l Joint Research / Invited
• [Presentation] 翻訳初期段階におけるペプチジルtRNAの脱落と校正機構 (2016)
  • Author(s): 鈴木 勉
  • Organizer: 第89回日本生化学会大会
  • Invited
• [Presentation] Molecular pathogenesis of mitochondrial diseases caused by tRNA modification deficiency (2016)
  • Author(s): Tsutomu Suzuki
  • Organizer: The 13th Conference of Asian Society for Mitochondrial Research and Medicine
  • Int'l Joint Research / Invited
• [Presentation] Molecular pathogenesis of human diseases caused by RNA modification deficiency (2016)
  • Author(s): Tsutomu Suzuki
  • Organizer: 第39回日本分子生物学会年会
  • Invited
• [Presentation] Post-transcriptional modifications in human mitochondrial tRNAs associated with pathological consequences (2015)
  • Author(s): Tsutomu Suzuki
  • Organizer: MitoClub
  • Place of Presentation: Cologne, Germany
  • Year and Date: 2015-11-18
  • Invited
• [Presentation] Molecular pathogenesis of mitochondrial disease caused by tRNA modification deficiency (2015)
  • Author(s): Tsutomu Suzuki
  • Organizer: 12th Conference of the Asian Society of Mitochondrial Research and Medicine (ASMRM)
  • Place of Presentation: Zhejiang, China
  • Year and Date: 2015-11-14
  • Invited
• [Presentation] Molecular pathogenesis of mitochondrial disease caused by tRNA modification deficiency (2015)
  • Author(s): Tsutomu Suzuki
  • Organizer: MitoCross Symposium 2015: mitochondria at the crossroad
  • Place of Presentation: Strasbourg, France
  • Year and Date: 2015-09-21
  • Invited
• [Presentation] RlmE-mediated methylation of 23S rRNA triggers late steps of 50S ribosomal subunit assembly (2015)
  • Author(s): Tsutomu Suzuki
  • Organizer: EMBO Conference on Ribosome Synthesis
  • Place of Presentation: Brussels, Belgium
  • Year and Date: 2015-08-19
• [Presentation] RNA修飾の多彩な機能と疾患 (2015)
  • Author(s): 鈴木 勉
  • Organizer: 名古屋市立大学薬学会特別講演会
  • Place of Presentation: 名古屋市立大、愛知県名古屋市
  • Year and Date: 2015-06-04
  • Invited
• [Presentation] 質量分析装置(MS)を使いこなす－核酸の修飾を見る－ (2015)
  • Author(s): 鈴木 勉
  • Organizer: 高知大学ESPO 教育プログラム
  • Place of Presentation: 高知大学、高知県高知市
  • Year and Date: 2015-06-03
  • Invited
• [Presentation] RNA修飾の生合成と機能―硫黄代謝との関連を中心に― (2015)
  • Author(s): 鈴木 勉
  • Organizer: 文部科学省 新学術領域研究（研究領域提案型）「酸素生物学」「第２回新学術領域技術支援セミナー」
  • Place of Presentation: 東北大学医学部、宮城県仙台市
  • Year and Date: 2015-04-30
  • Invited
• [Presentation] Essential RNA acetyltransferase required for ribosome biogenesis and tRNA modification (2015)
  • Author(s): Tsutomu Suzuki
  • Organizer: GRC on RNA editing and modification（招待講演）
  • Place of Presentation: Lucca, Italy
  • Year and Date: 2015-03-09
• [Presentation] RNA修飾の多彩な機能と疾患 (2015)
  • Author(s): 鈴木 勉
  • Organizer: 埼玉医科大学ゲノム医学研究センター学術集会（招待講演）
  • Place of Presentation: 埼玉医科大学ゲノム医学研究センター、埼玉県日高市
  • Year and Date: 2015-01-27
  • Invited
• [Presentation] Mitochondrial diseases caused by deficient tRNA modifications (2014)
  • Author(s): Tsutomu Suzuki
  • Organizer: AussieMit2014（招待講演）
  • Place of Presentation: Perth, Australia
  • Year and Date: 2014-12-01
  • Int'l Joint Research / Invited
• [Presentation] A single acetylation of 18S rRNA regulates ribosome biogenesis by sensing cellular energy status (2014)
  • Author(s): Tsutomu Suzuki
  • Organizer: Joint Australia and Japan RNA meeting (JAJRNA2014)（招待講演）
  • Place of Presentation: Sydney, Australia
  • Year and Date: 2014-11-05
  • Int'l Joint Research / Invited
• [Presentation] Biogenesis and function of cyclic t6A and derivatives (2014)
  • Author(s): Tsutomu Suzuki
  • Organizer: 25th tRNA conference（招待講演）
  • Place of Presentation: Kyllini, Greece
  • Year and Date: 2014-09-22
  • Int'l Joint Research / Invited
• [Presentation] ヒトはなぜ病気になるのでしょう？～病気の原因を分子レベルで探る～ (2014)
  • Author(s): 鈴木 勉
  • Organizer: 高校生のためのオープンキャンパス2014（招待講演）
  • Place of Presentation: 東京大学本郷キャンパス、東京都文京区
  • Year and Date: 2014-08-06
  • Invited
• [Book] RNA修飾の変動と生命現象／実験医学 2018年12月号 (2018)
  • Author(s): 鈴木 勉
  • Total Pages: 7
  • Publisher: 羊土社
  • ISBN: 9784758125147
• [Book] シュードウリジン化／生体の科学2018年10月号 (増大号) タンパク質・核酸の分子修飾 (2018)
  • Author(s): 北川 翔・鈴木 勉
  • Total Pages: 1
  • Publisher: 医学書院
• [Book] ヒドロキシル化／生体の科学2018年10月号 (増大号) タンパク質・核酸の分子修飾 (2018)
  • Author(s): 鈴木 健夫・鈴木 勉
  • Total Pages: 1
  • Publisher: 医学書院
• [Book] ミトコンドリアtRNA修飾の機能と疾患 (医学のあゆみ) (2017)
  • Author(s): 鈴木 勉
  • Total Pages: 8
  • Publisher: 医歯薬出版株式会社
• [Book] RNA修飾のケミカルバイオロジー (現代化学4月号) (2016)
  • Author(s): 鈴木 勉
  • Total Pages: 4
  • Publisher: 東京化学同人
• [Book] RNA修飾の多彩な機能と疾患 (ノンコーディングRNA-RNA分子の全体像を俯瞰する) (2016)
  • Author(s): 鈴木 勉, 大平 高之
  • Total Pages: 39
  • Publisher: 化学同人
• [Remarks] 意外な小分子がRNAの化学修飾に関与していた－酢酸イオンを基質とするtRNAアセチル化酵素の発見
  • URL: https://www.u-tokyo.ac.jp/focus/ja/articles/z0508_00075.html
• [Remarks] 炭酸ガスに敏感なRNAの化学修飾―tRNA修飾とワールブルク効果の関係性
  • URL: https://www.u-tokyo.ac.jp/focus/ja/articles/z0508_00026.html
• [Remarks] メチルは端だが役に立つ？ ―mRNA のキャップ構造における m6A 修飾酵素の同定
  • URL: https://www.t.u-tokyo.ac.jp/shared/press/data/setnws_201811270918295542170205_190437.pdf#search=%27%E3%83%A1%E3%83%81%E3%83%AB%E3%81%AF%E7%AB%AF%27
• [Remarks] RNAの化学修飾が遺伝暗号を変える 変則遺伝暗号の成立に関わる新規tRNA修飾塩基の発見
  • URL: https://www.u-tokyo.ac.jp/focus/ja/articles/a_00613.html
• [Remarks] 変則暗号の成立に寄与する新規のtRNA修飾の発見
  • URL: https://first.lifesciencedb.jp/16978
• [Remarks] 転移RNAの成熟を助ける意外な方法 出芽酵母のtRNA前駆体はキャップ修飾により安定化されている
  • URL: https://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/unexpected-modification-to-support-maturation-of-transfer-RNA.html
• [Remarks] tRNA前駆体は5’キャップ構造により安定化されている
  • URL: http://first.lifesciencedb.jp/archives/12799
• [Remarks] 変則的な暗号を解読するためのはじめの一歩 ミトコンドリアゲノムの解読に必須なメチル化酵素を同定
  • URL: https://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/first-step-to-deciphering-a-non-universal-genetic-code.html
• [Remarks] 変則的な遺伝暗号の解読をつかさどるf5C修飾はNSUN3によるメチル化からはじまる
  • URL: http://first.lifesciencedb.jp/archives/12537
• [Remarks] http://rna.chem.t.u-tokyo.ac.jp/
• [Remarks] http://rna.chem.t.u-tokyo.ac.jp/
• [Funded Workshop] Cold Spring Harbor Asia "RNA modification and epitranscriptome" (2017)
• [Funded Workshop] ConBio2017 "Emerging world of RNA modifications and epitranscriptome" (2017)